Enterobacteria phage WA13

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Bullavirinae; Alphatrevirus; unclassified Alphatrevirus; Enterobacteria phage WA13 sensu lato

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2LM85|Q2LM85_9VIRU Capsid protein F OS=Enterobacteria phage WA13 OX=384643 GN=F PE=3 SV=1
MM1 pKa = 6.59FQVYY5 pKa = 8.35VSKK8 pKa = 10.75HH9 pKa = 4.12VAPFTSVNRR18 pKa = 11.84AFSIAPAANPEE29 pKa = 4.26VVNSGEE35 pKa = 4.02IVAGRR40 pKa = 11.84STFRR44 pKa = 11.84IASSIASASNGSGFAFVQMMDD65 pKa = 3.86PNSPDD70 pKa = 3.28SQQVFSVAASLSFSVDD86 pKa = 2.94SNYY89 pKa = 9.87IPCVVRR95 pKa = 11.84FEE97 pKa = 4.53STVAQPTSFDD107 pKa = 2.91IATADD112 pKa = 4.75FIPVEE117 pKa = 4.29ASALSPRR124 pKa = 11.84ALSVRR129 pKa = 11.84DD130 pKa = 3.79CVTVDD135 pKa = 2.83VKK137 pKa = 11.05PRR139 pKa = 11.84TEE141 pKa = 4.03GNNVFVGVVFFSAAANAGNVTGVVSMAQVDD171 pKa = 4.17HH172 pKa = 6.84EE173 pKa = 4.72VTALQPLKK181 pKa = 10.96

Molecular weight: 18.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2LM87|Q2LM87_9VIRU GPE OS=Enterobacteria phage WA13 OX=384643 GN=E PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.16KK3 pKa = 10.29ARR5 pKa = 11.84RR6 pKa = 11.84SPSRR10 pKa = 11.84KK11 pKa = 8.81KK12 pKa = 10.43GARR15 pKa = 11.84LWYY18 pKa = 10.44VGGSQFF24 pKa = 4.73

Molecular weight: 2.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

1917

24

494

191.7

21.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.233 ± 1.34

1.043 ± 0.237

5.738 ± 0.436

4.903 ± 0.544

4.591 ± 0.692

5.529 ± 0.913

2.191 ± 0.367

3.965 ± 0.313

5.895 ± 0.95

8.451 ± 1.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.547 ± 0.378

4.434 ± 0.38

4.173 ± 0.692

5.373 ± 0.851

5.999 ± 0.684

7.981 ± 0.946

5.425 ± 0.359

6.729 ± 1.109

1.565 ± 0.284

3.234 ± 0.599
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski