Hubei tombus-like virus 28

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGB3|A0A1L3KGB3_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 28 OX=1923275 PE=4 SV=1
MM1 pKa = 7.26SVYY4 pKa = 9.84TGYY7 pKa = 8.59YY8 pKa = 8.57TPNNTTWYY16 pKa = 9.68QRR18 pKa = 11.84IGEE21 pKa = 4.31WVRR24 pKa = 11.84DD25 pKa = 3.46AMNYY29 pKa = 9.44NPEE32 pKa = 4.43LKK34 pKa = 10.15RR35 pKa = 11.84HH36 pKa = 5.09EE37 pKa = 4.84DD38 pKa = 3.19KK39 pKa = 11.59VEE41 pKa = 3.8EE42 pKa = 4.29LKK44 pKa = 11.31LKK46 pKa = 9.0ITEE49 pKa = 4.1NQNRR53 pKa = 11.84LLEE56 pKa = 4.65AEE58 pKa = 4.65LDD60 pKa = 3.6QHH62 pKa = 7.19AKK64 pKa = 10.55ALEE67 pKa = 4.17LDD69 pKa = 3.69AMKK72 pKa = 10.49VDD74 pKa = 3.73HH75 pKa = 7.29KK76 pKa = 11.07EE77 pKa = 3.64GLLAHH82 pKa = 6.75LKK84 pKa = 10.55FYY86 pKa = 10.28TLNKK90 pKa = 10.24KK91 pKa = 9.66IDD93 pKa = 3.76TKK95 pKa = 11.24YY96 pKa = 9.76IDD98 pKa = 3.64YY99 pKa = 8.62VTRR102 pKa = 11.84LAEE105 pKa = 4.45AYY107 pKa = 9.93FEE109 pKa = 4.32SMKK112 pKa = 10.48IKK114 pKa = 10.52NPRR117 pKa = 11.84LKK119 pKa = 10.62CEE121 pKa = 4.27LMDD124 pKa = 3.86EE125 pKa = 4.31VLPIHH130 pKa = 6.74IKK132 pKa = 10.17QRR134 pKa = 11.84LSTMPLLMKK143 pKa = 10.3DD144 pKa = 3.57DD145 pKa = 3.86RR146 pKa = 11.84TIASANYY153 pKa = 8.9ISEE156 pKa = 5.13LIDD159 pKa = 3.47GRR161 pKa = 11.84KK162 pKa = 9.34VSYY165 pKa = 9.34PWWNLWQPTYY175 pKa = 11.36ARR177 pKa = 11.84LGGSGNEE184 pKa = 3.98NADD187 pKa = 3.62YY188 pKa = 10.84LVPTQPNFWKK198 pKa = 10.64YY199 pKa = 11.0GMIVTGSVAIALGGKK214 pKa = 8.8WIISRR219 pKa = 11.84ASQNLTDD226 pKa = 6.04GITNLLAQKK235 pKa = 10.01AQQLPSTTVITQSLSKK251 pKa = 11.11DD252 pKa = 3.46MIEE255 pKa = 4.33IASKK259 pKa = 10.48GCTNIYY265 pKa = 10.86EE266 pKa = 4.28GMLWIWNSGHH276 pKa = 6.19VSNILPAFGQQASDD290 pKa = 3.18EE291 pKa = 4.63SIRR294 pKa = 11.84MIMTSFF300 pKa = 3.32

Molecular weight: 34.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGB3|A0A1L3KGB3_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 28 OX=1923275 PE=4 SV=1
MM1 pKa = 7.2VEE3 pKa = 5.24LMATDD8 pKa = 4.4LCQTGWIGKK17 pKa = 8.31RR18 pKa = 11.84EE19 pKa = 4.07CGLPRR24 pKa = 11.84THH26 pKa = 6.02TTQFLEE32 pKa = 3.97IRR34 pKa = 11.84NDD36 pKa = 3.07SYY38 pKa = 11.66RR39 pKa = 11.84IGSHH43 pKa = 5.94RR44 pKa = 11.84FGRR47 pKa = 11.84QMDD50 pKa = 4.22YY51 pKa = 10.59FPSIAKK57 pKa = 9.6PDD59 pKa = 2.95GWYY62 pKa = 10.26YY63 pKa = 10.94EE64 pKa = 4.56PIGSEE69 pKa = 3.81SAAAAFYY76 pKa = 10.66NRR78 pKa = 11.84HH79 pKa = 5.32NPEE82 pKa = 4.76LIEE85 pKa = 5.05GYY87 pKa = 10.2DD88 pKa = 3.56RR89 pKa = 11.84NCIKK93 pKa = 10.7GLYY96 pKa = 8.33KK97 pKa = 10.15HH98 pKa = 6.11LRR100 pKa = 11.84RR101 pKa = 11.84YY102 pKa = 8.69VVDD105 pKa = 3.66MEE107 pKa = 4.1QWTCEE112 pKa = 3.85QYY114 pKa = 10.54IASIWPASKK123 pKa = 10.39RR124 pKa = 11.84RR125 pKa = 11.84IYY127 pKa = 10.49QDD129 pKa = 5.34DD130 pKa = 3.83YY131 pKa = 11.95DD132 pKa = 4.12QFLSDD137 pKa = 4.25GKK139 pKa = 10.45ISSLVQPFTKK149 pKa = 10.03PEE151 pKa = 3.76KK152 pKa = 10.67KK153 pKa = 10.38NFSKK157 pKa = 11.09YY158 pKa = 9.4KK159 pKa = 8.81APRR162 pKa = 11.84MIQARR167 pKa = 11.84KK168 pKa = 8.99PIFNIHH174 pKa = 5.52YY175 pKa = 9.87GKK177 pKa = 10.4FIKK180 pKa = 10.23PLEE183 pKa = 4.08QKK185 pKa = 8.31ITKK188 pKa = 9.48HH189 pKa = 5.22GRR191 pKa = 11.84FRR193 pKa = 11.84DD194 pKa = 3.78HH195 pKa = 6.97FGKK198 pKa = 10.84GSPDD202 pKa = 3.18EE203 pKa = 3.79IARR206 pKa = 11.84RR207 pKa = 11.84ITKK210 pKa = 10.58LSLKK214 pKa = 8.63WQYY217 pKa = 9.02KK218 pKa = 8.29TEE220 pKa = 4.31GDD222 pKa = 3.49HH223 pKa = 5.52TTFDD227 pKa = 3.16AHH229 pKa = 5.58ITKK232 pKa = 10.22EE233 pKa = 4.08LLEE236 pKa = 4.43LTHH239 pKa = 6.8KK240 pKa = 10.68FYY242 pKa = 10.96AACYY246 pKa = 9.29NNNPEE251 pKa = 4.78LKK253 pKa = 9.46RR254 pKa = 11.84LSKK257 pKa = 9.82RR258 pKa = 11.84TIINRR263 pKa = 11.84CRR265 pKa = 11.84SRR267 pKa = 11.84HH268 pKa = 4.48GDD270 pKa = 2.85KK271 pKa = 10.19YY272 pKa = 8.56TVVGSRR278 pKa = 11.84MSGDD282 pKa = 3.12VDD284 pKa = 3.53TSFGNSIINIAILSEE299 pKa = 4.11LLYY302 pKa = 11.07KK303 pKa = 10.82LGINGEE309 pKa = 4.41FIVNGDD315 pKa = 3.78DD316 pKa = 6.19FILFTNKK323 pKa = 9.36PVNIKK328 pKa = 8.92MAEE331 pKa = 4.26AILRR335 pKa = 11.84TMNMEE340 pKa = 4.32TKK342 pKa = 9.61MKK344 pKa = 10.27EE345 pKa = 3.81STEE348 pKa = 4.22NIHH351 pKa = 6.2TVEE354 pKa = 4.64FCQTKK359 pKa = 10.36LVLTAEE365 pKa = 4.3GRR367 pKa = 11.84YY368 pKa = 6.09TTMKK372 pKa = 10.19HH373 pKa = 4.88IRR375 pKa = 11.84KK376 pKa = 7.08TVEE379 pKa = 3.63KK380 pKa = 10.42FGMTFVTTRR389 pKa = 11.84NYY391 pKa = 9.53WIYY394 pKa = 11.0VLEE397 pKa = 4.53NAHH400 pKa = 6.1GMWMMNKK407 pKa = 7.28TTPLGLLFKK416 pKa = 10.5HH417 pKa = 6.79IYY419 pKa = 10.26FKK421 pKa = 10.51MIEE424 pKa = 4.36LDD426 pKa = 3.37MVPNAKK432 pKa = 9.94EE433 pKa = 4.41LINKK437 pKa = 7.54HH438 pKa = 5.7HH439 pKa = 6.56RR440 pKa = 11.84LEE442 pKa = 4.09YY443 pKa = 10.39EE444 pKa = 3.83YY445 pKa = 11.25LEE447 pKa = 4.04RR448 pKa = 11.84AFIYY452 pKa = 9.5FIKK455 pKa = 10.54LARR458 pKa = 11.84KK459 pKa = 7.76DD460 pKa = 3.34TAMSDD465 pKa = 3.4DD466 pKa = 3.81HH467 pKa = 9.02SEE469 pKa = 3.84ITISMIAADD478 pKa = 4.76EE479 pKa = 4.33DD480 pKa = 4.47VMSLDD485 pKa = 4.66EE486 pKa = 5.51IEE488 pKa = 4.18QQLMQRR494 pKa = 11.84IIKK497 pKa = 10.03LYY499 pKa = 10.17NRR501 pKa = 11.84YY502 pKa = 7.19PTLSTKK508 pKa = 10.19QLLSMPYY515 pKa = 10.17NRR517 pKa = 11.84NLYY520 pKa = 9.43IDD522 pKa = 5.13HH523 pKa = 6.25NTKK526 pKa = 6.38TTPYY530 pKa = 9.48FVNHH534 pKa = 6.67

Molecular weight: 62.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

834

300

534

417.0

48.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.396 ± 0.739

1.079 ± 0.24

5.036 ± 0.021

6.475 ± 0.082

3.597 ± 1.122

5.036 ± 0.173

3.118 ± 0.65

8.153 ± 0.283

7.914 ± 0.337

8.633 ± 0.988

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.077 ± 0.045

5.516 ± 0.282

3.597 ± 0.04

3.477 ± 0.498

5.516 ± 0.881

5.516 ± 0.475

7.074 ± 0.237

3.477 ± 0.304

1.918 ± 0.629

5.396 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski