Streptomyces phage BRock

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Borockvirus; Streptomyces virus BRock

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0GVU1|A0A1J0GVU1_9CAUD Glycoside hydrolase OS=Streptomyces phage BRock OX=1913591 PE=3 SV=1
MM1 pKa = 7.25QLTITLDD8 pKa = 3.05TGLTYY13 pKa = 10.61SEE15 pKa = 4.32LVEE18 pKa = 4.21EE19 pKa = 4.33EE20 pKa = 4.51RR21 pKa = 11.84EE22 pKa = 3.86ISVPVNWIKK31 pKa = 10.19RR32 pKa = 11.84TWLNMRR38 pKa = 11.84GQTLPTVTVPTLVSVEE54 pKa = 4.22MPSLYY59 pKa = 9.93TIHH62 pKa = 7.79VDD64 pKa = 3.15EE65 pKa = 5.38DD66 pKa = 3.51TDD68 pKa = 3.79ADD70 pKa = 3.83EE71 pKa = 4.67FEE73 pKa = 4.22EE74 pKa = 5.63RR75 pKa = 11.84IISSLNPKK83 pKa = 9.38GNNHH87 pKa = 6.79SIVSLLWEE95 pKa = 4.27TDD97 pKa = 3.79FGPDD101 pKa = 3.95AEE103 pKa = 5.56LYY105 pKa = 9.72TPNQCPTGEE114 pKa = 3.99EE115 pKa = 3.75LANFVRR121 pKa = 11.84LMVDD125 pKa = 3.45GEE127 pKa = 4.66YY128 pKa = 10.88ASHH131 pKa = 6.68TPDD134 pKa = 3.03AYY136 pKa = 10.4AAAVSEE142 pKa = 4.46YY143 pKa = 10.99GWDD146 pKa = 3.45TGHH149 pKa = 7.82LSDD152 pKa = 5.88EE153 pKa = 4.38WFQDD157 pKa = 3.08NYY159 pKa = 10.25RR160 pKa = 11.84GQWEE164 pKa = 4.37SPEE167 pKa = 4.34EE168 pKa = 4.05YY169 pKa = 10.48VKK171 pKa = 11.12NLAEE175 pKa = 4.49DD176 pKa = 3.46MGAISPDD183 pKa = 3.39FPDD186 pKa = 4.69WIDD189 pKa = 3.53IDD191 pKa = 3.64WSGTADD197 pKa = 4.5NMSTDD202 pKa = 3.47DD203 pKa = 4.77DD204 pKa = 4.41YY205 pKa = 12.21VLFGLDD211 pKa = 2.92YY212 pKa = 10.99HH213 pKa = 6.57VFYY216 pKa = 11.08RR217 pKa = 11.84HH218 pKa = 5.49GG219 pKa = 3.49

Molecular weight: 25.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0GVR7|A0A1J0GVR7_9CAUD Uncharacterized protein OS=Streptomyces phage BRock OX=1913591 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84NYY4 pKa = 10.93SAMWDD9 pKa = 3.86DD10 pKa = 4.58FFGAALWVGFLFLLALILPVPGWPTLWWLTYY41 pKa = 10.17PFGAVLVGIVCRR53 pKa = 11.84KK54 pKa = 9.67LGWYY58 pKa = 9.94RR59 pKa = 11.84NLTIKK64 pKa = 9.94GWRR67 pKa = 11.84IQRR70 pKa = 11.84VNKK73 pKa = 10.28

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

188

0

188

32502

26

1025

172.9

19.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.381 ± 0.292

0.972 ± 0.102

6.237 ± 0.205

6.452 ± 0.298

3.483 ± 0.11

8.055 ± 0.3

1.88 ± 0.12

4.707 ± 0.15

4.707 ± 0.253

7.366 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.775 ± 0.111

4.581 ± 0.161

4.68 ± 0.149

3.686 ± 0.147

5.612 ± 0.173

6.117 ± 0.183

7.089 ± 0.267

7.39 ± 0.231

1.889 ± 0.108

3.941 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski