Amino acid dipepetide frequency for Exiguobacterium phage vB_EauS-123

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.882AlaAla: 5.882 ± 1.07
0.105AlaCys: 0.105 ± 0.107
4.831AlaAsp: 4.831 ± 0.662
6.302AlaGlu: 6.302 ± 0.917
3.361AlaPhe: 3.361 ± 0.562
4.936AlaGly: 4.936 ± 1.294
0.42AlaHis: 0.42 ± 0.185
5.567AlaIle: 5.567 ± 0.772
5.672AlaLys: 5.672 ± 0.853
6.617AlaLeu: 6.617 ± 0.772
1.68AlaMet: 1.68 ± 0.422
3.361AlaAsn: 3.361 ± 0.552
2.311AlaPro: 2.311 ± 0.449
3.571AlaGln: 3.571 ± 0.567
3.781AlaArg: 3.781 ± 0.832
4.096AlaSer: 4.096 ± 0.785
4.096AlaThr: 4.096 ± 0.755
4.411AlaVal: 4.411 ± 0.813
0.945AlaTrp: 0.945 ± 0.343
2.521AlaTyr: 2.521 ± 0.599
0.0AlaXaa: 0.0 ± 0.0
Cys
0.105CysAla: 0.105 ± 0.095
0.0CysCys: 0.0 ± 0.0
0.525CysAsp: 0.525 ± 0.246
0.735CysGlu: 0.735 ± 0.328
0.105CysPhe: 0.105 ± 0.126
0.525CysGly: 0.525 ± 0.285
0.105CysHis: 0.105 ± 0.102
0.21CysIle: 0.21 ± 0.139
0.21CysLys: 0.21 ± 0.121
0.315CysLeu: 0.315 ± 0.219
0.21CysMet: 0.21 ± 0.156
0.63CysAsn: 0.63 ± 0.249
0.0CysPro: 0.0 ± 0.0
0.42CysGln: 0.42 ± 0.199
0.525CysArg: 0.525 ± 0.29
0.315CysSer: 0.315 ± 0.189
0.0CysThr: 0.0 ± 0.0
0.21CysVal: 0.21 ± 0.137
0.0CysTrp: 0.0 ± 0.0
0.105CysTyr: 0.105 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
4.831AspAla: 4.831 ± 0.599
0.42AspCys: 0.42 ± 0.235
2.626AspAsp: 2.626 ± 0.521
5.777AspGlu: 5.777 ± 0.985
3.361AspPhe: 3.361 ± 0.682
5.252AspGly: 5.252 ± 0.585
1.155AspHis: 1.155 ± 0.395
4.096AspIle: 4.096 ± 0.612
3.991AspLys: 3.991 ± 0.476
4.831AspLeu: 4.831 ± 0.862
2.206AspMet: 2.206 ± 0.447
3.046AspAsn: 3.046 ± 0.731
1.891AspPro: 1.891 ± 0.352
2.521AspGln: 2.521 ± 0.418
3.256AspArg: 3.256 ± 0.612
3.466AspSer: 3.466 ± 0.399
2.521AspThr: 2.521 ± 0.472
4.306AspVal: 4.306 ± 0.597
1.05AspTrp: 1.05 ± 0.289
2.626AspTyr: 2.626 ± 0.483
0.0AspXaa: 0.0 ± 0.0
Glu
5.567GluAla: 5.567 ± 0.922
0.63GluCys: 0.63 ± 0.344
4.306GluAsp: 4.306 ± 0.945
6.302GluGlu: 6.302 ± 1.228
3.676GluPhe: 3.676 ± 0.704
3.571GluGly: 3.571 ± 0.588
0.945GluHis: 0.945 ± 0.305
4.201GluIle: 4.201 ± 0.585
6.512GluLys: 6.512 ± 0.976
6.932GluLeu: 6.932 ± 0.957
2.311GluMet: 2.311 ± 0.58
3.466GluAsn: 3.466 ± 0.569
1.575GluPro: 1.575 ± 0.407
3.886GluGln: 3.886 ± 0.682
5.882GluArg: 5.882 ± 0.994
3.046GluSer: 3.046 ± 0.572
5.147GluThr: 5.147 ± 0.827
5.357GluVal: 5.357 ± 1.137
0.945GluTrp: 0.945 ± 0.344
2.521GluTyr: 2.521 ± 0.473
0.0GluXaa: 0.0 ± 0.0
Phe
2.836PheAla: 2.836 ± 0.588
0.315PheCys: 0.315 ± 0.215
3.676PheAsp: 3.676 ± 0.659
4.096PheGlu: 4.096 ± 0.591
1.05PhePhe: 1.05 ± 0.421
2.731PheGly: 2.731 ± 0.481
0.525PheHis: 0.525 ± 0.223
2.521PheIle: 2.521 ± 0.515
3.256PheLys: 3.256 ± 0.598
2.206PheLeu: 2.206 ± 0.616
0.945PheMet: 0.945 ± 0.371
2.101PheAsn: 2.101 ± 0.488
1.26PhePro: 1.26 ± 0.347
1.996PheGln: 1.996 ± 0.473
1.68PheArg: 1.68 ± 0.412
1.891PheSer: 1.891 ± 0.451
2.101PheThr: 2.101 ± 0.477
2.731PheVal: 2.731 ± 0.689
0.21PheTrp: 0.21 ± 0.13
1.996PheTyr: 1.996 ± 0.467
0.0PheXaa: 0.0 ± 0.0
Gly
3.991GlyAla: 3.991 ± 0.735
0.105GlyCys: 0.105 ± 0.095
3.886GlyAsp: 3.886 ± 0.716
4.516GlyGlu: 4.516 ± 0.741
3.046GlyPhe: 3.046 ± 0.553
4.201GlyGly: 4.201 ± 1.051
0.735GlyHis: 0.735 ± 0.322
4.936GlyIle: 4.936 ± 0.7
4.516GlyLys: 4.516 ± 0.91
5.041GlyLeu: 5.041 ± 0.577
1.575GlyMet: 1.575 ± 0.37
3.466GlyAsn: 3.466 ± 0.576
1.05GlyPro: 1.05 ± 0.431
1.891GlyGln: 1.891 ± 0.546
3.361GlyArg: 3.361 ± 0.618
3.466GlySer: 3.466 ± 0.857
5.462GlyThr: 5.462 ± 1.001
4.306GlyVal: 4.306 ± 1.082
0.945GlyTrp: 0.945 ± 0.289
3.256GlyTyr: 3.256 ± 0.587
0.0GlyXaa: 0.0 ± 0.0
His
1.365HisAla: 1.365 ± 0.404
0.0HisCys: 0.0 ± 0.0
1.26HisAsp: 1.26 ± 0.448
1.786HisGlu: 1.786 ± 0.554
0.735HisPhe: 0.735 ± 0.251
1.155HisGly: 1.155 ± 0.332
0.315HisHis: 0.315 ± 0.196
1.365HisIle: 1.365 ± 0.385
1.05HisLys: 1.05 ± 0.415
1.575HisLeu: 1.575 ± 0.455
0.21HisMet: 0.21 ± 0.124
0.735HisAsn: 0.735 ± 0.281
0.21HisPro: 0.21 ± 0.143
0.315HisGln: 0.315 ± 0.186
1.05HisArg: 1.05 ± 0.357
0.735HisSer: 0.735 ± 0.307
0.525HisThr: 0.525 ± 0.231
1.26HisVal: 1.26 ± 0.401
0.105HisTrp: 0.105 ± 0.119
0.42HisTyr: 0.42 ± 0.231
0.0HisXaa: 0.0 ± 0.0
Ile
5.041IleAla: 5.041 ± 0.749
0.21IleCys: 0.21 ± 0.153
5.567IleAsp: 5.567 ± 0.774
6.827IleGlu: 6.827 ± 0.773
1.365IlePhe: 1.365 ± 0.401
4.201IleGly: 4.201 ± 0.678
1.155IleHis: 1.155 ± 0.289
4.411IleIle: 4.411 ± 0.754
4.621IleLys: 4.621 ± 0.698
3.886IleLeu: 3.886 ± 0.63
1.365IleMet: 1.365 ± 0.365
2.416IleAsn: 2.416 ± 0.509
2.626IlePro: 2.626 ± 0.659
1.891IleGln: 1.891 ± 0.443
3.466IleArg: 3.466 ± 0.539
2.941IleSer: 2.941 ± 0.556
4.936IleThr: 4.936 ± 0.888
4.621IleVal: 4.621 ± 0.713
0.315IleTrp: 0.315 ± 0.155
2.416IleTyr: 2.416 ± 0.366
0.0IleXaa: 0.0 ± 0.0
Lys
6.722LysAla: 6.722 ± 0.899
0.315LysCys: 0.315 ± 0.187
4.411LysAsp: 4.411 ± 0.674
4.096LysGlu: 4.096 ± 0.712
1.575LysPhe: 1.575 ± 0.657
4.726LysGly: 4.726 ± 0.644
1.365LysHis: 1.365 ± 0.463
3.571LysIle: 3.571 ± 0.601
5.777LysLys: 5.777 ± 0.719
6.722LysLeu: 6.722 ± 1.102
2.731LysMet: 2.731 ± 0.576
4.306LysAsn: 4.306 ± 0.536
1.891LysPro: 1.891 ± 0.391
4.411LysGln: 4.411 ± 0.863
4.831LysArg: 4.831 ± 0.886
3.781LysSer: 3.781 ± 0.656
4.936LysThr: 4.936 ± 0.824
5.147LysVal: 5.147 ± 0.812
0.84LysTrp: 0.84 ± 0.305
1.786LysTyr: 1.786 ± 0.477
0.0LysXaa: 0.0 ± 0.0
Leu
5.987LeuAla: 5.987 ± 0.631
0.84LeuCys: 0.84 ± 0.318
5.987LeuAsp: 5.987 ± 0.79
6.302LeuGlu: 6.302 ± 0.891
3.676LeuPhe: 3.676 ± 0.587
4.516LeuGly: 4.516 ± 0.733
2.101LeuHis: 2.101 ± 0.559
5.357LeuIle: 5.357 ± 0.723
5.252LeuLys: 5.252 ± 0.854
6.617LeuLeu: 6.617 ± 0.696
2.416LeuMet: 2.416 ± 0.569
4.096LeuAsn: 4.096 ± 0.617
2.206LeuPro: 2.206 ± 0.5
2.731LeuGln: 2.731 ± 0.478
4.201LeuArg: 4.201 ± 0.734
6.092LeuSer: 6.092 ± 0.759
5.777LeuThr: 5.777 ± 0.698
4.306LeuVal: 4.306 ± 0.574
0.525LeuTrp: 0.525 ± 0.229
2.206LeuTyr: 2.206 ± 0.489
0.0LeuXaa: 0.0 ± 0.0
Met
1.155MetAla: 1.155 ± 0.343
0.315MetCys: 0.315 ± 0.21
1.68MetAsp: 1.68 ± 0.486
1.365MetGlu: 1.365 ± 0.447
0.735MetPhe: 0.735 ± 0.277
1.155MetGly: 1.155 ± 0.41
0.21MetHis: 0.21 ± 0.118
1.786MetIle: 1.786 ± 0.381
2.941MetLys: 2.941 ± 0.55
1.786MetLeu: 1.786 ± 0.334
0.84MetMet: 0.84 ± 0.259
3.466MetAsn: 3.466 ± 0.709
1.26MetPro: 1.26 ± 0.321
0.945MetGln: 0.945 ± 0.331
1.155MetArg: 1.155 ± 0.434
1.26MetSer: 1.26 ± 0.341
2.731MetThr: 2.731 ± 0.562
1.05MetVal: 1.05 ± 0.256
0.21MetTrp: 0.21 ± 0.18
0.84MetTyr: 0.84 ± 0.268
0.0MetXaa: 0.0 ± 0.0
Asn
4.516AsnAla: 4.516 ± 0.584
0.21AsnCys: 0.21 ± 0.134
2.521AsnAsp: 2.521 ± 0.519
5.041AsnGlu: 5.041 ± 1.058
2.416AsnPhe: 2.416 ± 0.51
3.886AsnGly: 3.886 ± 0.642
1.155AsnHis: 1.155 ± 0.352
2.731AsnIle: 2.731 ± 0.594
3.151AsnLys: 3.151 ± 0.529
3.361AsnLeu: 3.361 ± 0.464
1.26AsnMet: 1.26 ± 0.411
2.836AsnAsn: 2.836 ± 0.611
2.941AsnPro: 2.941 ± 0.583
1.891AsnGln: 1.891 ± 0.384
2.416AsnArg: 2.416 ± 0.578
1.786AsnSer: 1.786 ± 0.5
3.151AsnThr: 3.151 ± 0.555
3.781AsnVal: 3.781 ± 0.564
1.05AsnTrp: 1.05 ± 0.39
1.05AsnTyr: 1.05 ± 0.409
0.0AsnXaa: 0.0 ± 0.0
Pro
1.891ProAla: 1.891 ± 0.405
0.105ProCys: 0.105 ± 0.095
1.05ProAsp: 1.05 ± 0.306
2.101ProGlu: 2.101 ± 0.577
1.26ProPhe: 1.26 ± 0.335
1.47ProGly: 1.47 ± 0.499
0.42ProHis: 0.42 ± 0.201
1.786ProIle: 1.786 ± 0.438
2.206ProLys: 2.206 ± 0.539
2.416ProLeu: 2.416 ± 0.447
0.63ProMet: 0.63 ± 0.258
1.891ProAsn: 1.891 ± 0.512
0.21ProPro: 0.21 ± 0.153
0.84ProGln: 0.84 ± 0.29
1.575ProArg: 1.575 ± 0.434
1.575ProSer: 1.575 ± 0.593
2.836ProThr: 2.836 ± 0.715
2.626ProVal: 2.626 ± 0.51
0.315ProTrp: 0.315 ± 0.218
1.155ProTyr: 1.155 ± 0.386
0.0ProXaa: 0.0 ± 0.0
Gln
3.466GlnAla: 3.466 ± 0.625
0.105GlnCys: 0.105 ± 0.087
2.836GlnAsp: 2.836 ± 0.553
2.836GlnGlu: 2.836 ± 0.542
1.47GlnPhe: 1.47 ± 0.374
1.996GlnGly: 1.996 ± 0.415
0.735GlnHis: 0.735 ± 0.29
2.206GlnIle: 2.206 ± 0.493
2.836GlnLys: 2.836 ± 0.587
2.941GlnLeu: 2.941 ± 0.537
1.47GlnMet: 1.47 ± 0.467
2.206GlnAsn: 2.206 ± 0.464
1.26GlnPro: 1.26 ± 0.384
1.26GlnGln: 1.26 ± 0.524
2.101GlnArg: 2.101 ± 0.492
1.786GlnSer: 1.786 ± 0.336
3.046GlnThr: 3.046 ± 0.659
2.941GlnVal: 2.941 ± 0.561
0.21GlnTrp: 0.21 ± 0.122
2.311GlnTyr: 2.311 ± 0.533
0.0GlnXaa: 0.0 ± 0.0
Arg
3.676ArgAla: 3.676 ± 0.793
0.525ArgCys: 0.525 ± 0.334
2.941ArgAsp: 2.941 ± 0.564
2.836ArgGlu: 2.836 ± 0.65
2.101ArgPhe: 2.101 ± 0.491
2.731ArgGly: 2.731 ± 0.574
1.26ArgHis: 1.26 ± 0.378
4.201ArgIle: 4.201 ± 0.518
5.252ArgLys: 5.252 ± 1.003
6.722ArgLeu: 6.722 ± 1.103
1.68ArgMet: 1.68 ± 0.459
1.786ArgAsn: 1.786 ± 0.593
1.47ArgPro: 1.47 ± 0.411
2.626ArgGln: 2.626 ± 0.557
2.836ArgArg: 2.836 ± 0.722
3.991ArgSer: 3.991 ± 0.518
2.731ArgThr: 2.731 ± 0.644
3.571ArgVal: 3.571 ± 0.798
0.63ArgTrp: 0.63 ± 0.266
2.101ArgTyr: 2.101 ± 0.509
0.0ArgXaa: 0.0 ± 0.0
Ser
3.991SerAla: 3.991 ± 0.576
0.105SerCys: 0.105 ± 0.102
2.941SerAsp: 2.941 ± 0.564
3.886SerGlu: 3.886 ± 0.761
3.466SerPhe: 3.466 ± 0.618
4.201SerGly: 4.201 ± 0.67
0.945SerHis: 0.945 ± 0.293
4.096SerIle: 4.096 ± 0.583
3.676SerLys: 3.676 ± 0.665
5.147SerLeu: 5.147 ± 0.717
1.365SerMet: 1.365 ± 0.41
2.626SerAsn: 2.626 ± 0.578
1.575SerPro: 1.575 ± 0.412
2.101SerGln: 2.101 ± 0.436
3.046SerArg: 3.046 ± 0.58
3.466SerSer: 3.466 ± 0.682
3.046SerThr: 3.046 ± 0.557
4.516SerVal: 4.516 ± 0.69
0.525SerTrp: 0.525 ± 0.234
2.206SerTyr: 2.206 ± 0.456
0.0SerXaa: 0.0 ± 0.0
Thr
6.302ThrAla: 6.302 ± 1.022
0.21ThrCys: 0.21 ± 0.148
3.886ThrAsp: 3.886 ± 0.666
4.306ThrGlu: 4.306 ± 0.663
2.731ThrPhe: 2.731 ± 0.51
4.726ThrGly: 4.726 ± 0.913
0.42ThrHis: 0.42 ± 0.219
4.726ThrIle: 4.726 ± 0.632
4.411ThrLys: 4.411 ± 0.666
5.462ThrLeu: 5.462 ± 0.866
1.05ThrMet: 1.05 ± 0.299
3.046ThrAsn: 3.046 ± 0.724
2.311ThrPro: 2.311 ± 0.616
1.786ThrGln: 1.786 ± 0.407
3.151ThrArg: 3.151 ± 0.512
4.411ThrSer: 4.411 ± 0.943
3.886ThrThr: 3.886 ± 0.93
4.726ThrVal: 4.726 ± 0.591
0.525ThrTrp: 0.525 ± 0.21
3.151ThrTyr: 3.151 ± 0.539
0.0ThrXaa: 0.0 ± 0.0
Val
4.726ValAla: 4.726 ± 0.8
0.105ValCys: 0.105 ± 0.114
4.411ValAsp: 4.411 ± 0.602
4.096ValGlu: 4.096 ± 0.732
2.731ValPhe: 2.731 ± 0.491
4.306ValGly: 4.306 ± 0.661
1.05ValHis: 1.05 ± 0.316
4.726ValIle: 4.726 ± 0.722
4.621ValLys: 4.621 ± 0.596
5.567ValLeu: 5.567 ± 0.832
1.26ValMet: 1.26 ± 0.408
3.046ValAsn: 3.046 ± 0.501
1.155ValPro: 1.155 ± 0.332
2.941ValGln: 2.941 ± 0.634
3.781ValArg: 3.781 ± 0.6
5.462ValSer: 5.462 ± 1.076
4.621ValThr: 4.621 ± 0.68
4.726ValVal: 4.726 ± 0.831
0.42ValTrp: 0.42 ± 0.207
3.781ValTyr: 3.781 ± 0.557
0.0ValXaa: 0.0 ± 0.0
Trp
0.315TrpAla: 0.315 ± 0.176
0.0TrpCys: 0.0 ± 0.0
0.525TrpAsp: 0.525 ± 0.213
0.63TrpGlu: 0.63 ± 0.246
0.315TrpPhe: 0.315 ± 0.17
0.63TrpGly: 0.63 ± 0.238
0.21TrpHis: 0.21 ± 0.128
0.525TrpIle: 0.525 ± 0.266
0.84TrpLys: 0.84 ± 0.288
0.525TrpLeu: 0.525 ± 0.244
0.21TrpMet: 0.21 ± 0.135
0.63TrpAsn: 0.63 ± 0.219
0.0TrpPro: 0.0 ± 0.0
0.63TrpGln: 0.63 ± 0.248
1.155TrpArg: 1.155 ± 0.427
0.945TrpSer: 0.945 ± 0.297
0.84TrpThr: 0.84 ± 0.238
0.525TrpVal: 0.525 ± 0.222
0.42TrpTrp: 0.42 ± 0.231
0.735TrpTyr: 0.735 ± 0.222
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.101TyrAla: 2.101 ± 0.406
0.63TyrCys: 0.63 ± 0.278
3.466TyrAsp: 3.466 ± 0.702
2.941TyrGlu: 2.941 ± 0.503
0.945TyrPhe: 0.945 ± 0.337
2.836TyrGly: 2.836 ± 0.44
0.945TyrHis: 0.945 ± 0.332
1.26TyrIle: 1.26 ± 0.42
3.151TyrLys: 3.151 ± 0.449
2.521TyrLeu: 2.521 ± 0.435
1.365TyrMet: 1.365 ± 0.371
1.996TyrAsn: 1.996 ± 0.557
1.155TyrPro: 1.155 ± 0.34
1.365TyrGln: 1.365 ± 0.398
2.416TyrArg: 2.416 ± 0.454
2.416TyrSer: 2.416 ± 0.513
2.836TyrThr: 2.836 ± 0.533
2.416TyrVal: 2.416 ± 0.484
0.42TyrTrp: 0.42 ± 0.209
1.26TyrTyr: 1.26 ± 0.425
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (9522 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski