Amino acid dipepetide frequency for Actinomyces slackii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.121AlaAla: 20.121 ± 0.252	1.229AlaCys: 1.229 ± 0.044	7.552AlaAsp: 7.552 ± 0.099	8.362AlaGlu: 8.362 ± 0.118	3.007AlaPhe: 3.007 ± 0.07	13.299AlaGly: 13.299 ± 0.168	2.756AlaHis: 2.756 ± 0.063	4.96AlaIle: 4.96 ± 0.085	2.319AlaLys: 2.319 ± 0.064	15.158AlaLeu: 15.158 ± 0.187	3.11AlaMet: 3.11 ± 0.055	1.976AlaAsn: 1.976 ± 0.055	7.89AlaPro: 7.89 ± 0.149	5.555AlaGln: 5.555 ± 0.106	10.22AlaArg: 10.22 ± 0.143	8.451AlaSer: 8.451 ± 0.114	7.216AlaThr: 7.216 ± 0.093	11.346AlaVal: 11.346 ± 0.134	2.135AlaTrp: 2.135 ± 0.052	2.311AlaTyr: 2.311 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	1.078CysAla: 1.078 ± 0.036	0.084CysCys: 0.084 ± 0.01	0.384CysAsp: 0.384 ± 0.025	0.424CysGlu: 0.424 ± 0.022	0.213CysPhe: 0.213 ± 0.015	0.809CysGly: 0.809 ± 0.035	0.179CysHis: 0.179 ± 0.016	0.228CysIle: 0.228 ± 0.015	0.079CysLys: 0.079 ± 0.009	0.914CysLeu: 0.914 ± 0.035	0.146CysMet: 0.146 ± 0.014	0.114CysAsn: 0.114 ± 0.011	0.45CysPro: 0.45 ± 0.027	0.299CysGln: 0.299 ± 0.018	0.507CysArg: 0.507 ± 0.024	0.494CysSer: 0.494 ± 0.026	0.459CysThr: 0.459 ± 0.029	0.542CysVal: 0.542 ± 0.028	0.119CysTrp: 0.119 ± 0.012	0.151CysTyr: 0.151 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.417AspAla: 7.417 ± 0.096	0.41AspCys: 0.41 ± 0.021	3.404AspAsp: 3.404 ± 0.074	3.821AspGlu: 3.821 ± 0.077	1.493AspPhe: 1.493 ± 0.041	5.274AspGly: 5.274 ± 0.104	1.334AspHis: 1.334 ± 0.042	2.042AspIle: 2.042 ± 0.051	1.059AspLys: 1.059 ± 0.04	6.308AspLeu: 6.308 ± 0.1	0.96AspMet: 0.96 ± 0.031	0.966AspAsn: 0.966 ± 0.037	4.443AspPro: 4.443 ± 0.074	1.781AspGln: 1.781 ± 0.046	3.733AspArg: 3.733 ± 0.072	3.337AspSer: 3.337 ± 0.071	2.581AspThr: 2.581 ± 0.06	4.241AspVal: 4.241 ± 0.074	0.894AspTrp: 0.894 ± 0.033	1.383AspTyr: 1.383 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	8.818GluAla: 8.818 ± 0.1	0.351GluCys: 0.351 ± 0.021	3.416GluAsp: 3.416 ± 0.067	4.002GluGlu: 4.002 ± 0.075	1.153GluPhe: 1.153 ± 0.04	4.717GluGly: 4.717 ± 0.079	1.511GluHis: 1.511 ± 0.045	2.6GluIle: 2.6 ± 0.054	0.976GluLys: 0.976 ± 0.043	6.236GluLeu: 6.236 ± 0.091	1.086GluMet: 1.086 ± 0.038	0.889GluAsn: 0.889 ± 0.033	3.649GluPro: 3.649 ± 0.077	2.334GluGln: 2.334 ± 0.057	5.184GluArg: 5.184 ± 0.09	3.02GluSer: 3.02 ± 0.061	2.722GluThr: 2.722 ± 0.056	4.957GluVal: 4.957 ± 0.078	0.615GluTrp: 0.615 ± 0.026	0.96GluTyr: 0.96 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	2.869PheAla: 2.869 ± 0.062	0.187PheCys: 0.187 ± 0.015	1.585PheAsp: 1.585 ± 0.049	1.318PheGlu: 1.318 ± 0.04	0.824PhePhe: 0.824 ± 0.036	2.211PheGly: 2.211 ± 0.055	0.55PheHis: 0.55 ± 0.023	1.183PheIle: 1.183 ± 0.04	0.459PheLys: 0.459 ± 0.021	2.356PheLeu: 2.356 ± 0.061	0.48PheMet: 0.48 ± 0.024	0.583PheAsn: 0.583 ± 0.024	1.21PhePro: 1.21 ± 0.038	0.618PheGln: 0.618 ± 0.025	1.351PheArg: 1.351 ± 0.045	1.672PheSer: 1.672 ± 0.041	1.805PheThr: 1.805 ± 0.045	1.808PheVal: 1.808 ± 0.05	0.332PheTrp: 0.332 ± 0.017	0.631PheTyr: 0.631 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	12.26GlyAla: 12.26 ± 0.16	0.695GlyCys: 0.695 ± 0.029	4.353GlyAsp: 4.353 ± 0.073	4.897GlyGlu: 4.897 ± 0.082	2.493GlyPhe: 2.493 ± 0.06	7.738GlyGly: 7.738 ± 0.112	1.981GlyHis: 1.981 ± 0.049	3.924GlyIle: 3.924 ± 0.074	1.905GlyLys: 1.905 ± 0.049	9.568GlyLeu: 9.568 ± 0.116	2.22GlyMet: 2.22 ± 0.05	1.503GlyAsn: 1.503 ± 0.054	4.893GlyPro: 4.893 ± 0.089	3.703GlyGln: 3.703 ± 0.081	7.02GlyArg: 7.02 ± 0.101	5.83GlySer: 5.83 ± 0.086	5.546GlyThr: 5.546 ± 0.101	7.06GlyVal: 7.06 ± 0.103	1.511GlyTrp: 1.511 ± 0.045	2.08GlyTyr: 2.08 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.53HisAla: 2.53 ± 0.063	0.21HisCys: 0.21 ± 0.013	1.324HisAsp: 1.324 ± 0.041	1.25HisGlu: 1.25 ± 0.038	0.422HisPhe: 0.422 ± 0.021	2.024HisGly: 2.024 ± 0.05	0.6HisHis: 0.6 ± 0.025	0.674HisIle: 0.674 ± 0.029	0.267HisLys: 0.267 ± 0.015	2.277HisLeu: 2.277 ± 0.058	0.379HisMet: 0.379 ± 0.02	0.407HisAsn: 0.407 ± 0.024	1.46HisPro: 1.46 ± 0.036	0.604HisGln: 0.604 ± 0.027	1.82HisArg: 1.82 ± 0.043	1.163HisSer: 1.163 ± 0.039	1.001HisThr: 1.001 ± 0.036	1.571HisVal: 1.571 ± 0.041	0.325HisTrp: 0.325 ± 0.018	0.496HisTyr: 0.496 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.62IleAla: 5.62 ± 0.086	0.347IleCys: 0.347 ± 0.022	2.934IleAsp: 2.934 ± 0.054	2.778IleGlu: 2.778 ± 0.055	0.949IlePhe: 0.949 ± 0.038	3.788IleGly: 3.788 ± 0.067	0.816IleHis: 0.816 ± 0.026	2.048IleIle: 2.048 ± 0.063	0.824IleLys: 0.824 ± 0.033	3.929IleLeu: 3.929 ± 0.086	0.817IleMet: 0.817 ± 0.027	0.979IleAsn: 0.979 ± 0.033	2.222IlePro: 2.222 ± 0.044	0.981IleGln: 0.981 ± 0.035	2.273IleArg: 2.273 ± 0.047	2.38IleSer: 2.38 ± 0.062	2.925IleThr: 2.925 ± 0.07	3.547IleVal: 3.547 ± 0.062	0.451IleTrp: 0.451 ± 0.02	0.73IleTyr: 0.73 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	2.523LysAla: 2.523 ± 0.065	0.084LysCys: 0.084 ± 0.009	1.25LysAsp: 1.25 ± 0.043	1.202LysGlu: 1.202 ± 0.049	0.305LysPhe: 0.305 ± 0.019	1.585LysGly: 1.585 ± 0.047	0.375LysHis: 0.375 ± 0.021	0.715LysIle: 0.715 ± 0.031	0.621LysLys: 0.621 ± 0.032	1.106LysLeu: 1.106 ± 0.042	0.301LysMet: 0.301 ± 0.017	0.439LysAsn: 0.439 ± 0.026	0.979LysPro: 0.979 ± 0.034	0.557LysGln: 0.557 ± 0.025	1.356LysArg: 1.356 ± 0.052	0.904LysSer: 0.904 ± 0.036	1.085LysThr: 1.085 ± 0.039	1.35LysVal: 1.35 ± 0.049	0.172LysTrp: 0.172 ± 0.013	0.372LysTyr: 0.372 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	15.399LeuAla: 15.399 ± 0.17	0.768LeuCys: 0.768 ± 0.029	6.128LeuAsp: 6.128 ± 0.102	5.762LeuGlu: 5.762 ± 0.087	2.039LeuPhe: 2.039 ± 0.048	9.451LeuGly: 9.451 ± 0.12	1.883LeuHis: 1.883 ± 0.05	4.334LeuIle: 4.334 ± 0.089	1.593LeuLys: 1.593 ± 0.047	10.25LeuLeu: 10.25 ± 0.139	2.037LeuMet: 2.037 ± 0.051	1.597LeuAsn: 1.597 ± 0.043	5.806LeuPro: 5.806 ± 0.089	2.022LeuGln: 2.022 ± 0.045	7.784LeuArg: 7.784 ± 0.117	6.384LeuSer: 6.384 ± 0.089	6.544LeuThr: 6.544 ± 0.089	8.729LeuVal: 8.729 ± 0.123	1.254LeuTrp: 1.254 ± 0.045	1.525LeuTyr: 1.525 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	2.771MetAla: 2.771 ± 0.067	0.116MetCys: 0.116 ± 0.012	1.056MetAsp: 1.056 ± 0.037	1.049MetGlu: 1.049 ± 0.031	0.422MetPhe: 0.422 ± 0.023	1.73MetGly: 1.73 ± 0.048	0.366MetHis: 0.366 ± 0.019	0.902MetIle: 0.902 ± 0.031	0.359MetLys: 0.359 ± 0.019	2.04MetLeu: 2.04 ± 0.05	0.412MetMet: 0.412 ± 0.023	0.446MetAsn: 0.446 ± 0.025	1.341MetPro: 1.341 ± 0.04	0.433MetGln: 0.433 ± 0.02	1.738MetArg: 1.738 ± 0.039	1.849MetSer: 1.849 ± 0.045	1.758MetThr: 1.758 ± 0.043	1.671MetVal: 1.671 ± 0.041	0.245MetTrp: 0.245 ± 0.017	0.256MetTyr: 0.256 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.085AsnAla: 2.085 ± 0.052	0.137AsnCys: 0.137 ± 0.011	0.939AsnAsp: 0.939 ± 0.035	0.928AsnGlu: 0.928 ± 0.033	0.399AsnPhe: 0.399 ± 0.024	1.573AsnGly: 1.573 ± 0.05	0.403AsnHis: 0.403 ± 0.021	0.742AsnIle: 0.742 ± 0.034	0.381AsnLys: 0.381 ± 0.026	1.728AsnLeu: 1.728 ± 0.048	0.329AsnMet: 0.329 ± 0.018	0.443AsnAsn: 0.443 ± 0.029	1.349AsnPro: 1.349 ± 0.043	0.591AsnGln: 0.591 ± 0.03	1.184AsnArg: 1.184 ± 0.031	0.958AsnSer: 0.958 ± 0.034	1.033AsnThr: 1.033 ± 0.038	1.24AsnVal: 1.24 ± 0.037	0.282AsnTrp: 0.282 ± 0.023	0.441AsnTyr: 0.441 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	8.765ProAla: 8.765 ± 0.157	0.31ProCys: 0.31 ± 0.021	3.821ProAsp: 3.821 ± 0.073	4.167ProGlu: 4.167 ± 0.069	1.315ProPhe: 1.315 ± 0.041	6.341ProGly: 6.341 ± 0.117	1.157ProHis: 1.157 ± 0.032	1.91ProIle: 1.91 ± 0.047	0.841ProLys: 0.841 ± 0.035	4.783ProLeu: 4.783 ± 0.078	1.139ProMet: 1.139 ± 0.031	0.896ProAsn: 0.896 ± 0.032	2.704ProPro: 2.704 ± 0.081	2.348ProGln: 2.348 ± 0.056	3.722ProArg: 3.722 ± 0.078	3.964ProSer: 3.964 ± 0.091	3.639ProThr: 3.639 ± 0.071	4.854ProVal: 4.854 ± 0.084	0.952ProTrp: 0.952 ± 0.03	1.06ProTyr: 1.06 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	5.67GlnAla: 5.67 ± 0.095	0.224GlnCys: 0.224 ± 0.018	2.068GlnAsp: 2.068 ± 0.058	2.2GlnGlu: 2.2 ± 0.047	0.597GlnPhe: 0.597 ± 0.023	3.216GlnGly: 3.216 ± 0.071	0.579GlnHis: 0.579 ± 0.027	1.386GlnIle: 1.386 ± 0.038	0.39GlnLys: 0.39 ± 0.023	2.396GlnLeu: 2.396 ± 0.052	0.673GlnMet: 0.673 ± 0.028	0.453GlnAsn: 0.453 ± 0.028	1.828GlnPro: 1.828 ± 0.062	1.015GlnGln: 1.015 ± 0.038	2.641GlnArg: 2.641 ± 0.057	1.616GlnSer: 1.616 ± 0.047	1.542GlnThr: 1.542 ± 0.045	2.928GlnVal: 2.928 ± 0.064	0.562GlnTrp: 0.562 ± 0.025	0.519GlnTyr: 0.519 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	10.08ArgAla: 10.08 ± 0.143	0.48ArgCys: 0.48 ± 0.023	3.766ArgAsp: 3.766 ± 0.077	4.217ArgGlu: 4.217 ± 0.078	1.955ArgPhe: 1.955 ± 0.051	5.39ArgGly: 5.39 ± 0.085	1.74ArgHis: 1.74 ± 0.048	3.419ArgIle: 3.419 ± 0.06	1.203ArgLys: 1.203 ± 0.041	8.244ArgLeu: 8.244 ± 0.123	1.805ArgMet: 1.805 ± 0.044	1.168ArgAsn: 1.168 ± 0.036	4.406ArgPro: 4.406 ± 0.088	2.58ArgGln: 2.58 ± 0.047	7.386ArgArg: 7.386 ± 0.125	4.701ArgSer: 4.701 ± 0.076	4.21ArgThr: 4.21 ± 0.065	5.352ArgVal: 5.352 ± 0.082	1.183ArgTrp: 1.183 ± 0.038	1.451ArgTyr: 1.451 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	8.503SerAla: 8.503 ± 0.113	0.465SerCys: 0.465 ± 0.028	2.805SerAsp: 2.805 ± 0.067	2.758SerGlu: 2.758 ± 0.068	1.808SerPhe: 1.808 ± 0.048	6.29SerGly: 6.29 ± 0.086	1.178SerHis: 1.178 ± 0.041	2.387SerIle: 2.387 ± 0.051	0.997SerLys: 0.997 ± 0.032	6.184SerLeu: 6.184 ± 0.086	1.412SerMet: 1.412 ± 0.041	0.989SerAsn: 0.989 ± 0.034	4.051SerPro: 4.051 ± 0.081	2.385SerGln: 2.385 ± 0.057	4.476SerArg: 4.476 ± 0.074	4.325SerSer: 4.325 ± 0.09	4.178SerThr: 4.178 ± 0.076	4.408SerVal: 4.408 ± 0.074	1.057SerTrp: 1.057 ± 0.035	1.253SerTyr: 1.253 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	8.11ThrAla: 8.11 ± 0.113	0.565ThrCys: 0.565 ± 0.031	3.159ThrAsp: 3.159 ± 0.069	2.871ThrGlu: 2.871 ± 0.058	1.51ThrPhe: 1.51 ± 0.047	6.118ThrGly: 6.118 ± 0.087	1.159ThrHis: 1.159 ± 0.035	2.776ThrIle: 2.776 ± 0.06	1.017ThrLys: 1.017 ± 0.036	5.39ThrLeu: 5.39 ± 0.084	1.229ThrMet: 1.229 ± 0.041	1.075ThrAsn: 1.075 ± 0.041	3.704ThrPro: 3.704 ± 0.072	1.694ThrGln: 1.694 ± 0.044	3.799ThrArg: 3.799 ± 0.073	3.63ThrSer: 3.63 ± 0.08	4.211ThrThr: 4.211 ± 0.085	5.38ThrVal: 5.38 ± 0.095	0.999ThrTrp: 0.999 ± 0.038	1.261ThrTyr: 1.261 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	10.318ValAla: 10.318 ± 0.12	0.675ValCys: 0.675 ± 0.03	4.874ValAsp: 4.874 ± 0.082	5.293ValGlu: 5.293 ± 0.086	2.142ValPhe: 2.142 ± 0.052	6.812ValGly: 6.812 ± 0.099	1.555ValHis: 1.555 ± 0.043	3.824ValIle: 3.824 ± 0.079	1.384ValLys: 1.384 ± 0.04	8.735ValLeu: 8.735 ± 0.133	1.709ValMet: 1.709 ± 0.048	1.458ValAsn: 1.458 ± 0.043	4.531ValPro: 4.531 ± 0.067	1.793ValGln: 1.793 ± 0.041	5.733ValArg: 5.733 ± 0.095	5.077ValSer: 5.077 ± 0.082	5.161ValThr: 5.161 ± 0.09	8.229ValVal: 8.229 ± 0.126	1.047ValTrp: 1.047 ± 0.039	1.448ValTyr: 1.448 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.713TrpAla: 1.713 ± 0.05	0.155TrpCys: 0.155 ± 0.013	0.885TrpAsp: 0.885 ± 0.035	0.759TrpGlu: 0.759 ± 0.03	0.427TrpPhe: 0.427 ± 0.022	1.064TrpGly: 1.064 ± 0.034	0.293TrpHis: 0.293 ± 0.019	0.598TrpIle: 0.598 ± 0.029	0.279TrpLys: 0.279 ± 0.017	1.638TrpLeu: 1.638 ± 0.043	0.414TrpMet: 0.414 ± 0.022	0.352TrpAsn: 0.352 ± 0.018	0.725TrpPro: 0.725 ± 0.03	0.59TrpGln: 0.59 ± 0.026	1.273TrpArg: 1.273 ± 0.039	0.995TrpSer: 0.995 ± 0.039	0.861TrpThr: 0.861 ± 0.029	1.104TrpVal: 1.104 ± 0.039	0.353TrpTrp: 0.353 ± 0.023	0.317TrpTyr: 0.317 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.321TyrAla: 2.321 ± 0.055	0.181TyrCys: 0.181 ± 0.014	1.18TyrAsp: 1.18 ± 0.035	1.136TyrGlu: 1.136 ± 0.034	0.561TyrPhe: 0.561 ± 0.025	1.752TyrGly: 1.752 ± 0.043	0.388TyrHis: 0.388 ± 0.02	0.683TyrIle: 0.683 ± 0.027	0.33TyrLys: 0.33 ± 0.022	2.048TyrLeu: 2.048 ± 0.053	0.302TyrMet: 0.302 ± 0.017	0.433TyrAsn: 0.433 ± 0.023	1.049TyrPro: 1.049 ± 0.038	0.622TyrGln: 0.622 ± 0.025	1.536TyrArg: 1.536 ± 0.045	1.161TyrSer: 1.161 ± 0.037	1.179TyrThr: 1.179 ± 0.05	1.479TyrVal: 1.479 ± 0.046	0.306TyrTrp: 0.306 ± 0.018	0.505TyrTyr: 0.505 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2571 proteins (929870 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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