Helicobacter pylori bacteriophage KHP30

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; Helicobacter virus KHP30

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7H894|I7H894_BPKHP Putative terminase OS=Helicobacter pylori bacteriophage KHP30 OX=1208236 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.82EE5 pKa = 4.23KK6 pKa = 10.55EE7 pKa = 3.99IEE9 pKa = 4.18LEE11 pKa = 3.99TLKK14 pKa = 11.01RR15 pKa = 11.84EE16 pKa = 3.99IAQAEE21 pKa = 4.06ASLEE25 pKa = 3.89QDD27 pKa = 4.59FIKK30 pKa = 10.97HH31 pKa = 5.29MVDD34 pKa = 2.81KK35 pKa = 10.6TNEE38 pKa = 3.95KK39 pKa = 10.6VEE41 pKa = 4.57DD42 pKa = 4.1LFFSDD47 pKa = 4.65KK48 pKa = 10.89PEE50 pKa = 4.12FYY52 pKa = 10.68KK53 pKa = 10.92FVFTEE58 pKa = 3.7QNNYY62 pKa = 9.57LRR64 pKa = 11.84EE65 pKa = 4.0KK66 pKa = 10.26LTDD69 pKa = 3.66KK70 pKa = 10.75VSKK73 pKa = 11.02AMDD76 pKa = 3.95LSDD79 pKa = 4.78EE80 pKa = 4.11IQRR83 pKa = 11.84DD84 pKa = 3.19KK85 pKa = 11.29DD86 pKa = 3.41AEE88 pKa = 4.22EE89 pKa = 4.12IEE91 pKa = 4.25KK92 pKa = 10.88DD93 pKa = 3.22KK94 pKa = 11.39QAFLNKK100 pKa = 9.88HH101 pKa = 6.01PEE103 pKa = 3.66VDD105 pKa = 4.17FNEE108 pKa = 4.04LLEE111 pKa = 4.69FYY113 pKa = 10.7EE114 pKa = 4.23EE115 pKa = 4.16EE116 pKa = 3.89LPKK119 pKa = 10.38RR120 pKa = 11.84IKK122 pKa = 9.34TQIDD126 pKa = 3.75KK127 pKa = 11.53LEE129 pKa = 4.17GAAFFEE135 pKa = 5.83AILDD139 pKa = 3.72YY140 pKa = 11.16FNAINARR147 pKa = 11.84EE148 pKa = 3.97EE149 pKa = 4.21EE150 pKa = 4.18PKK152 pKa = 10.44KK153 pKa = 10.35EE154 pKa = 4.23SKK156 pKa = 10.6EE157 pKa = 4.24EE158 pKa = 3.95YY159 pKa = 10.21SSLPKK164 pKa = 10.09EE165 pKa = 3.88ALGNGVSGVGYY176 pKa = 10.77ANNEE180 pKa = 4.31NIMTRR185 pKa = 11.84YY186 pKa = 9.53

Molecular weight: 21.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7GUS8|I7GUS8_BPKHP ddrB-ParB domain-containing protein OS=Helicobacter pylori bacteriophage KHP30 OX=1208236 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84SLIYY6 pKa = 10.37KK7 pKa = 8.88HH8 pKa = 6.48ANRR11 pKa = 11.84NFKK14 pKa = 10.27PKK16 pKa = 9.52VWTNSVLLEE25 pKa = 4.06GGILEE30 pKa = 4.2ILEE33 pKa = 4.03ALKK36 pKa = 10.82AIEE39 pKa = 4.09NRR41 pKa = 11.84SRR43 pKa = 11.84SQVLEE48 pKa = 3.58RR49 pKa = 11.84LIIFFIEE56 pKa = 4.26TQKK59 pKa = 11.35GQTNEE64 pKa = 4.25KK65 pKa = 9.24AWEE68 pKa = 3.99RR69 pKa = 11.84SKK71 pKa = 10.84RR72 pKa = 11.84AYY74 pKa = 9.73KK75 pKa = 9.43RR76 pKa = 11.84TLTNQTKK83 pKa = 10.39KK84 pKa = 10.48NQLKK88 pKa = 10.04RR89 pKa = 11.84KK90 pKa = 7.27QLEE93 pKa = 4.19RR94 pKa = 11.84VAKK97 pKa = 9.59AQKK100 pKa = 10.17KK101 pKa = 9.5KK102 pKa = 10.47QLQANANHH110 pKa = 6.18SFSFFKK116 pKa = 10.74RR117 pKa = 11.84SS118 pKa = 2.94

Molecular weight: 14.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

8183

43

1633

272.8

31.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.55 ± 0.481

0.77 ± 0.258

5.059 ± 0.346

8.188 ± 0.335

4.778 ± 0.323

3.959 ± 0.277

1.283 ± 0.145

7.063 ± 0.315

10.852 ± 0.445

10.4 ± 0.362

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.165

8.371 ± 0.489

2.334 ± 0.168

4.803 ± 0.493

4.069 ± 0.265

6.672 ± 0.273

4.803 ± 0.247

4.069 ± 0.352

0.501 ± 0.108

3.678 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski