Streptococcus phage Javan255

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A5B9|A0A4D6A5B9_9CAUD VRR-NUC domain-containing protein OS=Streptococcus phage Javan255 OX=2548075 GN=Javan255_0038 PE=4 SV=1
MM1 pKa = 7.08TRR3 pKa = 11.84AEE5 pKa = 4.27LNEE8 pKa = 4.52IIDD11 pKa = 3.65TCFIHH16 pKa = 7.44LMVMKK21 pKa = 9.86QHH23 pKa = 5.73YY24 pKa = 9.23SKK26 pKa = 10.96SRR28 pKa = 11.84EE29 pKa = 3.68FALDD33 pKa = 3.57VIEE36 pKa = 5.37QEE38 pKa = 4.66NLNQINDD45 pKa = 4.15LLDD48 pKa = 5.57DD49 pKa = 3.59ITSGIEE55 pKa = 3.49RR56 pKa = 11.84GGFTEE61 pKa = 6.27LEE63 pKa = 4.08ACCIYY68 pKa = 11.11DD69 pKa = 3.35DD70 pKa = 5.48TEE72 pKa = 4.35FLWSEE77 pKa = 3.88VSKK80 pKa = 10.8EE81 pKa = 3.98FEE83 pKa = 4.12KK84 pKa = 11.1VGYY87 pKa = 9.87

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A6T3|A0A4D6A6T3_9CAUD Site-specific recombinase OS=Streptococcus phage Javan255 OX=2548075 GN=Javan255_0001 PE=4 SV=1
MM1 pKa = 7.57FDD3 pKa = 4.44IILSYY8 pKa = 10.88ILIGVYY14 pKa = 9.92IFLGLYY20 pKa = 9.24FNYY23 pKa = 9.91MLIRR27 pKa = 11.84EE28 pKa = 5.29DD29 pKa = 2.95IRR31 pKa = 11.84QEE33 pKa = 3.98KK34 pKa = 9.14KK35 pKa = 10.38RR36 pKa = 11.84KK37 pKa = 9.47AEE39 pKa = 4.08KK40 pKa = 10.33KK41 pKa = 9.12LQEE44 pKa = 4.47SNSTPLRR51 pKa = 11.84RR52 pKa = 11.84SRR54 pKa = 3.74

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12261

41

1472

272.5

30.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.957 ± 0.434

0.979 ± 0.184

5.595 ± 0.282

7.455 ± 0.301

3.85 ± 0.193

6.476 ± 0.273

1.957 ± 0.159

5.995 ± 0.244

7.438 ± 0.38

9.314 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.259 ± 0.179

4.323 ± 0.19

3.026 ± 0.197

4.69 ± 0.204

4.763 ± 0.281

6.867 ± 0.339

6.892 ± 0.606

6.419 ± 0.191

1.37 ± 0.107

3.377 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski