Clostridium phage CPD7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Guelinviridae; Susfortunavirus; unclassified Susfortunavirus

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5H2TTB9|A0A5H2TTB9_9CAUD Major capsid protein OS=Clostridium phage CPD7 OX=2483608 GN=CPD7_06 PE=4 SV=1
MM1 pKa = 7.28LTYY4 pKa = 10.59KK5 pKa = 10.89GGDD8 pKa = 3.19KK9 pKa = 10.54LLKK12 pKa = 10.0VDD14 pKa = 3.31YY15 pKa = 9.94HH16 pKa = 7.69LNIEE20 pKa = 4.36VTQGDD25 pKa = 4.05SGCLFFGSNPYY36 pKa = 10.99SFMGSEE42 pKa = 4.28EE43 pKa = 4.02LRR45 pKa = 11.84LEE47 pKa = 4.32LFLGDD52 pKa = 4.61EE53 pKa = 4.88IIFSKK58 pKa = 10.53KK59 pKa = 10.39SNIVDD64 pKa = 3.45RR65 pKa = 11.84GLLFLEE71 pKa = 4.77LTEE74 pKa = 4.15QQ75 pKa = 3.62

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5H2TJX5|A0A5H2TJX5_9CAUD N-acetylmuramoyl-L-alanine amidase OS=Clostridium phage CPD7 OX=2483608 GN=CPD7_12 PE=4 SV=1
MM1 pKa = 7.54FKK3 pKa = 10.31ILFCNHH9 pKa = 4.89EE10 pKa = 4.68LIRR13 pKa = 11.84FAFVIRR19 pKa = 11.84DD20 pKa = 3.15GKK22 pKa = 9.64MYY24 pKa = 10.73KK25 pKa = 10.3LSEE28 pKa = 4.23CVHH31 pKa = 5.92CGKK34 pKa = 10.18RR35 pKa = 11.84RR36 pKa = 11.84IEE38 pKa = 4.42VIVKK42 pKa = 9.87

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

5736

42

799

229.4

26.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.801 ± 0.45

1.377 ± 0.214

6.241 ± 0.289

8.525 ± 0.534

4.603 ± 0.557

6.869 ± 0.815

1.464 ± 0.112

7.636 ± 0.282

9.222 ± 0.661

8.211 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.145

7.81 ± 0.465

2.999 ± 0.459

3.208 ± 0.303

3.905 ± 0.303

5.178 ± 0.553

4.637 ± 0.333

5.544 ± 0.294

1.046 ± 0.192

5.526 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski