Propionibacterium phage phiPA50S

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pahexavirus; unclassified Pahexavirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5FSY0|A0A7D5FSY0_9CAUD Putative excinuclease OS=Propionibacterium phage phiPA50S OX=2749186 PE=4 SV=1
MM1 pKa = 8.13DD2 pKa = 3.9NTHH5 pKa = 7.08NIPYY9 pKa = 8.11TALKK13 pKa = 9.07TVVHH17 pKa = 7.13RR18 pKa = 11.84IIQQQPTNMQQLEE31 pKa = 4.4NIVDD35 pKa = 4.0GVEE38 pKa = 3.67NQYY41 pKa = 10.54RR42 pKa = 11.84VPISLDD48 pKa = 3.34NVNLTVKK55 pKa = 10.14EE56 pKa = 4.09VSLDD60 pKa = 3.69DD61 pKa = 4.6LAIDD65 pKa = 4.03QDD67 pKa = 4.19TLDD70 pKa = 4.0EE71 pKa = 4.51CSEE74 pKa = 4.21ILWDD78 pKa = 4.54CDD80 pKa = 3.54SAGYY84 pKa = 9.25PNNSNTRR91 pKa = 11.84GIPDD95 pKa = 3.63DD96 pKa = 3.59TRR98 pKa = 11.84ASQEE102 pKa = 4.19AIDD105 pKa = 4.0WLAGIAYY112 pKa = 8.03QAKK115 pKa = 9.97LLQAEE120 pKa = 5.02ADD122 pKa = 4.71DD123 pKa = 3.99IMQSIICHH131 pKa = 6.21RR132 pKa = 11.84DD133 pKa = 2.68NYY135 pKa = 10.92KK136 pKa = 10.74NVIGQNVLHH145 pKa = 6.05QANDD149 pKa = 4.04TISACIHH156 pKa = 6.61LDD158 pKa = 3.35QLIEE162 pKa = 3.97EE163 pKa = 4.95TIDD166 pKa = 3.59SNEE169 pKa = 3.89SS170 pKa = 2.99

Molecular weight: 19.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5JHJ2|A0A7D5JHJ2_9CAUD Uncharacterized protein OS=Propionibacterium phage phiPA50S OX=2749186 PE=4 SV=1
MM1 pKa = 7.25HH2 pKa = 7.63RR3 pKa = 11.84VGDD6 pKa = 4.04HH7 pKa = 5.85GVKK10 pKa = 9.68TGPNRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84CLTVDD22 pKa = 3.02EE23 pKa = 4.91MEE25 pKa = 4.64AKK27 pKa = 10.38GMQRR31 pKa = 11.84NKK33 pKa = 10.34LGYY36 pKa = 5.86WTSGGTSYY44 pKa = 9.67WAKK47 pKa = 10.26EE48 pKa = 4.33AITPSHH54 pKa = 5.76TRR56 pKa = 11.84IAHH59 pKa = 4.91NRR61 pKa = 3.38

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

8275

61

921

201.8

22.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.248 ± 0.711

1.136 ± 0.178

6.683 ± 0.547

4.894 ± 0.424

2.767 ± 0.255

8.858 ± 0.59

2.477 ± 0.381

4.749 ± 0.405

4.085 ± 0.384

7.42 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.332

3.468 ± 0.311

4.955 ± 0.376

4.133 ± 0.321

6.139 ± 0.413

6.502 ± 0.389

5.958 ± 0.585

8.218 ± 0.866

1.897 ± 0.24

2.707 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski