Proteome-pI 2.0 - Proteome Isoelectric Point Database

Archaeoglobus profundus (strain DSM 5631 / JCM 9629 / NBRC 100127 / Av18)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae; Archaeoglobus; Archaeoglobus profundus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1822 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|D2RHT1|D2RHT1_ARCPA Uncharacterized protein OS=Archaeoglobus profundus (strain DSM 5631 / JCM 9629 / NBRC 100127 / Av18) OX=572546 GN=Arcpr_0793 PE=4 SV=1

MM1 pKa = 7.36EE2 pKa = 5.3RR3 pKa = 11.84ATTLVLVVLSIFAIGFNAPAFSTLVEE29 pKa = 4.96DD30 pKa = 3.95IVNGVTLTSDD40 pKa = 3.13SVYY43 pKa = 10.5ATTSNGNLTIDD54 pKa = 3.46ISVNNPNYY62 pKa = 9.8PGYY65 pKa = 11.13GDD67 pKa = 4.13GLSSGSVYY75 pKa = 10.63RR76 pKa = 11.84FDD78 pKa = 5.08DD79 pKa = 4.72VIVLEE84 pKa = 4.48NNVSRR89 pKa = 11.84TGEE92 pKa = 4.07SVVCVNIQSNDD103 pKa = 3.55DD104 pKa = 3.59SLKK107 pKa = 10.73FYY109 pKa = 9.85TDD111 pKa = 4.0SIAPSEE117 pKa = 4.6SINLTLGEE125 pKa = 4.43NEE127 pKa = 4.35SKK129 pKa = 10.91SVGIEE134 pKa = 3.99INSSTSGLGLVKK146 pKa = 10.4GSYY149 pKa = 9.24TIYY152 pKa = 10.41VYY154 pKa = 10.78SGEE157 pKa = 4.33CQQ159 pKa = 2.94

Molecular weight: 16.85 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|D2RG83|D2RG83_ARCPA DNA-binding protein Arcpr_0238 OS=Archaeoglobus profundus (strain DSM 5631 / JCM 9629 / NBRC 100127 / Av18) OX=572546 GN=Arcpr_0238 PE=3 SV=1

MM1 pKa = 7.34GKK3 pKa = 8.35KK4 pKa = 8.21TVGVKK9 pKa = 10.01RR10 pKa = 11.84RR11 pKa = 11.84LAKK14 pKa = 9.96FLKK17 pKa = 10.23ANRR20 pKa = 11.84RR21 pKa = 11.84PPLWVIIKK29 pKa = 7.02TNRR32 pKa = 11.84RR33 pKa = 11.84FVRR36 pKa = 11.84SPKK39 pKa = 8.51QRR41 pKa = 11.84HH42 pKa = 4.22WRR44 pKa = 11.84HH45 pKa = 5.66KK46 pKa = 9.44KK47 pKa = 10.54LKK49 pKa = 9.4VV50 pKa = 3.09

Molecular weight: 6.15 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1822	0	1822	478743	30	1707	262.8	29.8

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.466 ± 0.059	1.304 ± 0.027	5.322 ± 0.041	8.57 ± 0.069	4.488 ± 0.047	6.633 ± 0.053	1.513 ± 0.023	8.29 ± 0.042	8.232 ± 0.065	9.747 ± 0.063

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.269 ± 0.029	3.521 ± 0.034	3.716 ± 0.042	1.575 ± 0.023	5.71 ± 0.052	5.021 ± 0.045	4.143 ± 0.043	8.672 ± 0.056	0.993 ± 0.024	3.816 ± 0.042

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski