Barnesiella viscericola DSM 18177

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Barnesiellaceae; Barnesiella; Barnesiella viscericola

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2487 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0EQY6|W0EQY6_9BACT 50S ribosomal protein L10 OS=Barnesiella viscericola DSM 18177 OX=880074 GN=BARVI_11085 PE=3 SV=1
MM1 pKa = 7.5ARR3 pKa = 11.84NIIDD7 pKa = 5.41LICNSCSCGKK17 pKa = 10.43EE18 pKa = 3.76EE19 pKa = 4.17AQEE22 pKa = 4.0YY23 pKa = 10.71LDD25 pKa = 5.32DD26 pKa = 5.17EE27 pKa = 4.38IRR29 pKa = 11.84NLQEE33 pKa = 3.85LQEE36 pKa = 5.0DD37 pKa = 3.63NDD39 pKa = 3.71LRR41 pKa = 11.84SEE43 pKa = 4.16DD44 pKa = 4.68FEE46 pKa = 4.52IACSNLGLDD55 pKa = 4.42QDD57 pKa = 3.69WQIYY61 pKa = 8.32FINRR65 pKa = 11.84LAGLL69 pKa = 3.89

Molecular weight: 8.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0EWG0|W0EWG0_9BACT OMP_b-brl domain-containing protein OS=Barnesiella viscericola DSM 18177 OX=880074 GN=BARVI_00970 PE=4 SV=1
MM1 pKa = 7.75AEE3 pKa = 3.87YY4 pKa = 9.34PINKK8 pKa = 9.85GIGRR12 pKa = 11.84PVEE15 pKa = 4.26FKK17 pKa = 10.88GLKK20 pKa = 9.19AQYY23 pKa = 11.01LFIFCGGLLALFVLFVILYY42 pKa = 7.89MVGIDD47 pKa = 3.15QWICIGFGAASSSLLVWQTFALNARR72 pKa = 11.84YY73 pKa = 9.81GEE75 pKa = 4.33HH76 pKa = 7.16GLMKK80 pKa = 10.56LGAARR85 pKa = 11.84SHH87 pKa = 6.18PRR89 pKa = 11.84YY90 pKa = 9.86LINRR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84ITRR99 pKa = 11.84LFKK102 pKa = 10.36RR103 pKa = 11.84QRR105 pKa = 11.84KK106 pKa = 7.46EE107 pKa = 3.53EE108 pKa = 4.07RR109 pKa = 11.84QQ110 pKa = 3.35

Molecular weight: 12.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2487

0

2487

885770

30

2480

356.2

40.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.599 ± 0.05

1.229 ± 0.018

5.706 ± 0.038

6.53 ± 0.045

4.421 ± 0.031

6.856 ± 0.047

1.972 ± 0.022

6.663 ± 0.045

5.607 ± 0.055

9.31 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.022

4.576 ± 0.034

4.067 ± 0.024

3.494 ± 0.029

5.339 ± 0.044

6.123 ± 0.035

5.658 ± 0.04

6.735 ± 0.046

1.224 ± 0.019

4.363 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski