Siphonobacter aquaeclarae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Siphonobacter

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9SIY5|A0A1G9SIY5_9BACT Type III pantothenate kinase OS=Siphonobacter aquaeclarae OX=563176 GN=coaX PE=3 SV=1
MM1 pKa = 7.58KK2 pKa = 10.35LFRR5 pKa = 11.84YY6 pKa = 7.84VTEE9 pKa = 4.05EE10 pKa = 3.85DD11 pKa = 4.26VIPGILIDD19 pKa = 4.17GKK21 pKa = 9.77PYY23 pKa = 9.84SLEE26 pKa = 3.95GFFEE30 pKa = 4.6AFGEE34 pKa = 4.27EE35 pKa = 3.81TGAYY39 pKa = 10.23DD40 pKa = 3.52LDD42 pKa = 3.77EE43 pKa = 4.5YY44 pKa = 11.18FFEE47 pKa = 4.63EE48 pKa = 3.95EE49 pKa = 4.52GIAVLEE55 pKa = 5.17DD56 pKa = 3.46YY57 pKa = 11.06LEE59 pKa = 4.24QYY61 pKa = 7.54GTSEE65 pKa = 5.14LEE67 pKa = 4.19PLDD70 pKa = 5.2EE71 pKa = 5.62DD72 pKa = 3.41IQYY75 pKa = 10.3ASCVARR81 pKa = 11.84PSKK84 pKa = 10.33IICIGLNYY92 pKa = 9.74ADD94 pKa = 4.19HH95 pKa = 6.94AKK97 pKa = 8.59EE98 pKa = 3.88TGAPIPQEE106 pKa = 3.73PIIFFKK112 pKa = 10.58ATTALCGPNDD122 pKa = 3.79DD123 pKa = 4.28VVIPRR128 pKa = 11.84NSVKK132 pKa = 10.08TDD134 pKa = 3.12WEE136 pKa = 4.2VEE138 pKa = 4.01LAVVIGAEE146 pKa = 3.99CSYY149 pKa = 9.7VTEE152 pKa = 4.29EE153 pKa = 4.26QAVDD157 pKa = 3.93YY158 pKa = 10.44IAGYY162 pKa = 11.1AVMNDD167 pKa = 3.12YY168 pKa = 11.04SEE170 pKa = 6.23RR171 pKa = 11.84EE172 pKa = 3.94FQLEE176 pKa = 5.49RR177 pKa = 11.84GGQWDD182 pKa = 3.74KK183 pKa = 11.9GKK185 pKa = 11.04GCDD188 pKa = 3.22TFGPLGPYY196 pKa = 10.06LVTPDD201 pKa = 4.01EE202 pKa = 4.43VDD204 pKa = 4.57DD205 pKa = 4.31VDD207 pKa = 5.95SLPMWLKK214 pKa = 10.91VNGKK218 pKa = 6.67TFQNSTTAQMIFKK231 pKa = 9.13VPQVISYY238 pKa = 9.64LSQFMTLLPGDD249 pKa = 4.39VISTGTPPGVGLGFNPPIYY268 pKa = 10.07LKK270 pKa = 10.69PGDD273 pKa = 3.93VVEE276 pKa = 5.18LGIEE280 pKa = 4.12GLGEE284 pKa = 3.81QRR286 pKa = 11.84QVAVSWEE293 pKa = 4.03DD294 pKa = 3.34ANN296 pKa = 4.42

Molecular weight: 32.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9RIP6|A0A1G9RIP6_9BACT Uncharacterized protein OS=Siphonobacter aquaeclarae OX=563176 GN=SAMN04488090_2901 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.23RR3 pKa = 11.84LSAFFLFLTLSASAQTPEE21 pKa = 3.08QWYY24 pKa = 9.89RR25 pKa = 11.84RR26 pKa = 11.84EE27 pKa = 3.97TGSRR31 pKa = 11.84LSSPNRR37 pKa = 11.84HH38 pKa = 5.9MLPDD42 pKa = 3.69SLFSGAFFRR51 pKa = 11.84CRR53 pKa = 11.84PLPAAHH59 pKa = 7.26PSLMAGASRR68 pKa = 11.84LACLSDD74 pKa = 3.85EE75 pKa = 4.57LSAIHH80 pKa = 5.83TRR82 pKa = 11.84IVQAQGEE89 pKa = 4.55TGNQIRR95 pKa = 11.84LAKK98 pKa = 10.45LLLDD102 pKa = 3.72VRR104 pKa = 11.84RR105 pKa = 11.84QAVEE109 pKa = 3.68QYY111 pKa = 11.56ALLRR115 pKa = 11.84NAAKK119 pKa = 10.46RR120 pKa = 11.84LPLVRR125 pKa = 11.84KK126 pKa = 9.67RR127 pKa = 11.84LNTYY131 pKa = 10.08VFDD134 pKa = 4.62GSCALTRR141 pKa = 11.84YY142 pKa = 10.09DD143 pKa = 4.46GLITEE148 pKa = 5.37LLGTAPPPIVPP159 pKa = 4.23

Molecular weight: 17.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4964

0

4964

1846786

38

2407

372.0

41.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.462 ± 0.033

0.724 ± 0.009

5.218 ± 0.024

5.704 ± 0.037

4.776 ± 0.024

7.671 ± 0.033

1.852 ± 0.017

5.464 ± 0.027

5.136 ± 0.03

10.095 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.976 ± 0.013

4.238 ± 0.033

4.621 ± 0.021

3.791 ± 0.018

5.758 ± 0.029

6.223 ± 0.03

6.004 ± 0.031

6.979 ± 0.027

1.45 ± 0.013

3.857 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski