Amino acid dipepetide frequency for Actinoplanes brasiliensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.524AlaAla: 22.524 ± 0.168	0.924AlaCys: 0.924 ± 0.018	8.347AlaAsp: 8.347 ± 0.072	8.394AlaGlu: 8.394 ± 0.067	3.453AlaPhe: 3.453 ± 0.038	14.582AlaGly: 14.582 ± 0.124	2.469AlaHis: 2.469 ± 0.031	4.226AlaIle: 4.226 ± 0.047	2.52AlaLys: 2.52 ± 0.04	14.182AlaLeu: 14.182 ± 0.097	2.625AlaMet: 2.625 ± 0.035	2.305AlaAsn: 2.305 ± 0.036	6.614AlaPro: 6.614 ± 0.067	3.771AlaGln: 3.771 ± 0.041	9.842AlaArg: 9.842 ± 0.073	5.962AlaSer: 5.962 ± 0.053	7.798AlaThr: 7.798 ± 0.066	12.491AlaVal: 12.491 ± 0.093	1.892AlaTrp: 1.892 ± 0.029	2.805AlaTyr: 2.805 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	0.863CysAla: 0.863 ± 0.021	0.089CysCys: 0.089 ± 0.006	0.436CysAsp: 0.436 ± 0.014	0.31CysGlu: 0.31 ± 0.012	0.193CysPhe: 0.193 ± 0.008	0.79CysGly: 0.79 ± 0.018	0.165CysHis: 0.165 ± 0.008	0.149CysIle: 0.149 ± 0.008	0.1CysLys: 0.1 ± 0.007	0.672CysLeu: 0.672 ± 0.018	0.091CysMet: 0.091 ± 0.005	0.138CysAsn: 0.138 ± 0.008	0.453CysPro: 0.453 ± 0.016	0.153CysGln: 0.153 ± 0.008	0.575CysArg: 0.575 ± 0.016	0.401CysSer: 0.401 ± 0.012	0.41CysThr: 0.41 ± 0.014	0.535CysVal: 0.535 ± 0.015	0.107CysTrp: 0.107 ± 0.006	0.134CysTyr: 0.134 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.211AspAla: 7.211 ± 0.08	0.349AspCys: 0.349 ± 0.011	4.001AspAsp: 4.001 ± 0.046	3.992AspGlu: 3.992 ± 0.039	1.593AspPhe: 1.593 ± 0.023	6.111AspGly: 6.111 ± 0.062	1.258AspHis: 1.258 ± 0.022	1.811AspIle: 1.811 ± 0.03	1.16AspLys: 1.16 ± 0.026	6.495AspLeu: 6.495 ± 0.056	0.795AspMet: 0.795 ± 0.019	1.181AspAsn: 1.181 ± 0.023	4.566AspPro: 4.566 ± 0.039	1.722AspGln: 1.722 ± 0.025	4.995AspArg: 4.995 ± 0.051	2.441AspSer: 2.441 ± 0.032	2.842AspThr: 2.842 ± 0.031	5.021AspVal: 5.021 ± 0.046	0.94AspTrp: 0.94 ± 0.023	1.135AspTyr: 1.135 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	6.819GluAla: 6.819 ± 0.065	0.304GluCys: 0.304 ± 0.011	2.249GluAsp: 2.249 ± 0.032	2.495GluGlu: 2.495 ± 0.034	1.556GluPhe: 1.556 ± 0.027	3.453GluGly: 3.453 ± 0.041	1.371GluHis: 1.371 ± 0.023	2.333GluIle: 2.333 ± 0.034	1.218GluLys: 1.218 ± 0.028	6.395GluLeu: 6.395 ± 0.053	0.957GluMet: 0.957 ± 0.022	0.998GluAsn: 0.998 ± 0.021	3.597GluPro: 3.597 ± 0.052	2.305GluGln: 2.305 ± 0.03	4.907GluArg: 4.907 ± 0.053	2.496GluSer: 2.496 ± 0.032	2.783GluThr: 2.783 ± 0.035	4.437GluVal: 4.437 ± 0.04	0.737GluTrp: 0.737 ± 0.016	0.943GluTyr: 0.943 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.855PheAla: 3.855 ± 0.041	0.238PheCys: 0.238 ± 0.009	2.124PheAsp: 2.124 ± 0.03	1.359PheGlu: 1.359 ± 0.023	0.865PhePhe: 0.865 ± 0.019	3.064PheGly: 3.064 ± 0.033	0.591PheHis: 0.591 ± 0.015	0.712PheIle: 0.712 ± 0.017	0.483PheLys: 0.483 ± 0.013	2.648PheLeu: 2.648 ± 0.034	0.358PheMet: 0.358 ± 0.012	0.605PheAsn: 0.605 ± 0.016	1.444PhePro: 1.444 ± 0.023	0.698PheGln: 0.698 ± 0.015	1.923PheArg: 1.923 ± 0.028	1.436PheSer: 1.436 ± 0.023	2.013PheThr: 2.013 ± 0.031	2.441PheVal: 2.441 ± 0.028	0.488PheTrp: 0.488 ± 0.013	0.631PheTyr: 0.631 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	10.567GlyAla: 10.567 ± 0.073	0.733GlyCys: 0.733 ± 0.019	5.451GlyAsp: 5.451 ± 0.051	4.852GlyGlu: 4.852 ± 0.042	2.972GlyPhe: 2.972 ± 0.035	8.543GlyGly: 8.543 ± 0.081	2.174GlyHis: 2.174 ± 0.039	3.385GlyIle: 3.385 ± 0.04	2.261GlyLys: 2.261 ± 0.035	9.563GlyLeu: 9.563 ± 0.071	1.877GlyMet: 1.877 ± 0.031	2.012GlyAsn: 2.012 ± 0.03	5.115GlyPro: 5.115 ± 0.06	2.695GlyGln: 2.695 ± 0.035	7.676GlyArg: 7.676 ± 0.07	5.343GlySer: 5.343 ± 0.049	5.446GlyThr: 5.446 ± 0.062	7.957GlyVal: 7.957 ± 0.08	1.797GlyTrp: 1.797 ± 0.028	2.254GlyTyr: 2.254 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.469HisAla: 2.469 ± 0.036	0.159HisCys: 0.159 ± 0.008	1.384HisAsp: 1.384 ± 0.024	1.141HisGlu: 1.141 ± 0.021	0.572HisPhe: 0.572 ± 0.016	2.059HisGly: 2.059 ± 0.027	0.62HisHis: 0.62 ± 0.018	0.629HisIle: 0.629 ± 0.016	0.304HisLys: 0.304 ± 0.01	2.318HisLeu: 2.318 ± 0.03	0.302HisMet: 0.302 ± 0.011	0.411HisAsn: 0.411 ± 0.013	1.655HisPro: 1.655 ± 0.025	0.602HisGln: 0.602 ± 0.015	1.889HisArg: 1.889 ± 0.03	0.918HisSer: 0.918 ± 0.017	1.149HisThr: 1.149 ± 0.022	1.667HisVal: 1.667 ± 0.028	0.322HisTrp: 0.322 ± 0.01	0.433HisTyr: 0.433 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	5.39IleAla: 5.39 ± 0.053	0.287IleCys: 0.287 ± 0.01	2.541IleAsp: 2.541 ± 0.031	2.175IleGlu: 2.175 ± 0.029	0.895IlePhe: 0.895 ± 0.018	3.743IleGly: 3.743 ± 0.047	0.582IleHis: 0.582 ± 0.013	1.075IleIle: 1.075 ± 0.025	0.774IleLys: 0.774 ± 0.019	2.726IleLeu: 2.726 ± 0.036	0.512IleMet: 0.512 ± 0.015	0.869IleAsn: 0.869 ± 0.018	1.812IlePro: 1.812 ± 0.029	0.75IleGln: 0.75 ± 0.017	2.449IleArg: 2.449 ± 0.033	1.944IleSer: 1.944 ± 0.028	2.468IleThr: 2.468 ± 0.034	3.249IleVal: 3.249 ± 0.039	0.452IleTrp: 0.452 ± 0.014	0.625IleTyr: 0.625 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.528LysAla: 2.528 ± 0.039	0.111LysCys: 0.111 ± 0.007	0.947LysAsp: 0.947 ± 0.023	0.813LysGlu: 0.813 ± 0.022	0.484LysPhe: 0.484 ± 0.014	1.373LysGly: 1.373 ± 0.027	0.408LysHis: 0.408 ± 0.013	0.856LysIle: 0.856 ± 0.021	0.613LysLys: 0.613 ± 0.022	2.024LysLeu: 2.024 ± 0.035	0.369LysMet: 0.369 ± 0.013	0.481LysAsn: 0.481 ± 0.016	1.397LysPro: 1.397 ± 0.029	0.71LysGln: 0.71 ± 0.018	1.443LysArg: 1.443 ± 0.028	1.061LysSer: 1.061 ± 0.021	1.174LysThr: 1.174 ± 0.025	1.745LysVal: 1.745 ± 0.03	0.286LysTrp: 0.286 ± 0.009	0.427LysTyr: 0.427 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.529LeuAla: 15.529 ± 0.108	0.688LeuCys: 0.688 ± 0.019	6.714LeuAsp: 6.714 ± 0.063	4.356LeuGlu: 4.356 ± 0.044	2.733LeuPhe: 2.733 ± 0.034	9.164LeuGly: 9.164 ± 0.073	2.195LeuHis: 2.195 ± 0.03	4.085LeuIle: 4.085 ± 0.044	1.689LeuLys: 1.689 ± 0.032	11.291LeuLeu: 11.291 ± 0.09	1.575LeuMet: 1.575 ± 0.022	1.92LeuAsn: 1.92 ± 0.026	6.443LeuPro: 6.443 ± 0.054	2.235LeuGln: 2.235 ± 0.028	8.825LeuArg: 8.825 ± 0.06	5.542LeuSer: 5.542 ± 0.046	7.216LeuThr: 7.216 ± 0.056	8.849LeuVal: 8.849 ± 0.069	1.311LeuTrp: 1.311 ± 0.023	1.708LeuTyr: 1.708 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	2.265MetAla: 2.265 ± 0.033	0.096MetCys: 0.096 ± 0.005	0.764MetAsp: 0.764 ± 0.016	0.599MetGlu: 0.599 ± 0.015	0.496MetPhe: 0.496 ± 0.014	1.133MetGly: 1.133 ± 0.022	0.317MetHis: 0.317 ± 0.011	0.782MetIle: 0.782 ± 0.018	0.366MetLys: 0.366 ± 0.011	1.842MetLeu: 1.842 ± 0.03	0.309MetMet: 0.309 ± 0.012	0.402MetAsn: 0.402 ± 0.012	1.19MetPro: 1.19 ± 0.022	0.468MetGln: 0.468 ± 0.013	1.554MetArg: 1.554 ± 0.025	1.296MetSer: 1.296 ± 0.024	1.632MetThr: 1.632 ± 0.024	1.333MetVal: 1.333 ± 0.022	0.213MetTrp: 0.213 ± 0.008	0.287MetTyr: 0.287 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.435AsnAla: 2.435 ± 0.04	0.167AsnCys: 0.167 ± 0.009	1.125AsnAsp: 1.125 ± 0.024	0.918AsnGlu: 0.918 ± 0.021	0.562AsnPhe: 0.562 ± 0.015	2.172AsnGly: 2.172 ± 0.042	0.384AsnHis: 0.384 ± 0.014	0.666AsnIle: 0.666 ± 0.016	0.405AsnLys: 0.405 ± 0.013	1.924AsnLeu: 1.924 ± 0.025	0.318AsnMet: 0.318 ± 0.012	0.557AsnAsn: 0.557 ± 0.018	1.571AsnPro: 1.571 ± 0.028	0.644AsnGln: 0.644 ± 0.016	1.532AsnArg: 1.532 ± 0.026	1.025AsnSer: 1.025 ± 0.021	1.215AsnThr: 1.215 ± 0.027	1.581AsnVal: 1.581 ± 0.025	0.347AsnTrp: 0.347 ± 0.01	0.449AsnTyr: 0.449 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	9.636ProAla: 9.636 ± 0.08	0.276ProCys: 0.276 ± 0.011	4.344ProAsp: 4.344 ± 0.043	3.745ProGlu: 3.745 ± 0.04	1.551ProPhe: 1.551 ± 0.023	6.76ProGly: 6.76 ± 0.074	1.172ProHis: 1.172 ± 0.026	1.834ProIle: 1.834 ± 0.024	0.985ProLys: 0.985 ± 0.024	5.116ProLeu: 5.116 ± 0.052	1.012ProMet: 1.012 ± 0.018	1.032ProAsn: 1.032 ± 0.022	4.05ProPro: 4.05 ± 0.067	1.61ProGln: 1.61 ± 0.029	3.906ProArg: 3.906 ± 0.05	3.494ProSer: 3.494 ± 0.045	3.353ProThr: 3.353 ± 0.053	5.553ProVal: 5.553 ± 0.047	0.913ProTrp: 0.913 ± 0.02	1.294ProTyr: 1.294 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.94GlnAla: 3.94 ± 0.056	0.139GlnCys: 0.139 ± 0.008	1.187GlnAsp: 1.187 ± 0.021	1.227GlnGlu: 1.227 ± 0.024	0.822GlnPhe: 0.822 ± 0.019	1.948GlnGly: 1.948 ± 0.033	0.657GlnHis: 0.657 ± 0.015	1.243GlnIle: 1.243 ± 0.022	0.531GlnLys: 0.531 ± 0.016	3.108GlnLeu: 3.108 ± 0.034	0.503GlnMet: 0.503 ± 0.013	0.579GlnAsn: 0.579 ± 0.015	1.917GlnPro: 1.917 ± 0.036	1.23GlnGln: 1.23 ± 0.026	2.582GlnArg: 2.582 ± 0.036	1.28GlnSer: 1.28 ± 0.024	1.426GlnThr: 1.426 ± 0.027	2.638GlnVal: 2.638 ± 0.036	0.528GlnTrp: 0.528 ± 0.015	0.558GlnTyr: 0.558 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	9.608ArgAla: 9.608 ± 0.075	0.543ArgCys: 0.543 ± 0.016	4.606ArgAsp: 4.606 ± 0.048	4.333ArgGlu: 4.333 ± 0.047	2.402ArgPhe: 2.402 ± 0.031	5.557ArgGly: 5.557 ± 0.059	2.079ArgHis: 2.079 ± 0.03	3.153ArgIle: 3.153 ± 0.041	1.544ArgLys: 1.544 ± 0.032	9.179ArgLeu: 9.179 ± 0.081	1.796ArgMet: 1.796 ± 0.026	1.583ArgAsn: 1.583 ± 0.025	5.079ArgPro: 5.079 ± 0.052	2.43ArgGln: 2.43 ± 0.028	8.08ArgArg: 8.08 ± 0.079	4.196ArgSer: 4.196 ± 0.041	4.829ArgThr: 4.829 ± 0.054	6.164ArgVal: 6.164 ± 0.052	1.457ArgTrp: 1.457 ± 0.025	1.77ArgTyr: 1.77 ± 0.024	0.0ArgXaa: 0.0 ± 0.0
Ser	6.985SerAla: 6.985 ± 0.065	0.367SerCys: 0.367 ± 0.012	2.759SerAsp: 2.759 ± 0.033	2.376SerGlu: 2.376 ± 0.036	1.55SerPhe: 1.55 ± 0.025	5.841SerGly: 5.841 ± 0.06	0.966SerHis: 0.966 ± 0.018	1.812SerIle: 1.812 ± 0.028	0.93SerLys: 0.93 ± 0.023	4.824SerLeu: 4.824 ± 0.04	1.098SerMet: 1.098 ± 0.02	0.994SerAsn: 0.994 ± 0.024	3.355SerPro: 3.355 ± 0.043	1.293SerGln: 1.293 ± 0.028	3.954SerArg: 3.954 ± 0.039	3.104SerSer: 3.104 ± 0.047	3.18SerThr: 3.18 ± 0.039	4.189SerVal: 4.189 ± 0.041	0.979SerTrp: 0.979 ± 0.02	1.185SerTyr: 1.185 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	8.627ThrAla: 8.627 ± 0.074	0.39ThrCys: 0.39 ± 0.014	3.392ThrAsp: 3.392 ± 0.042	2.904ThrGlu: 2.904 ± 0.035	1.742ThrPhe: 1.742 ± 0.029	6.588ThrGly: 6.588 ± 0.061	1.066ThrHis: 1.066 ± 0.02	2.2ThrIle: 2.2 ± 0.036	1.083ThrLys: 1.083 ± 0.024	5.821ThrLeu: 5.821 ± 0.051	1.006ThrMet: 1.006 ± 0.018	1.197ThrAsn: 1.197 ± 0.025	4.201ThrPro: 4.201 ± 0.047	1.345ThrGln: 1.345 ± 0.025	4.007ThrArg: 4.007 ± 0.042	3.231ThrSer: 3.231 ± 0.037	4.08ThrThr: 4.08 ± 0.058	6.107ThrVal: 6.107 ± 0.053	0.927ThrTrp: 0.927 ± 0.022	1.278ThrTyr: 1.278 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	12.036ValAla: 12.036 ± 0.079	0.619ValCys: 0.619 ± 0.016	5.129ValAsp: 5.129 ± 0.052	4.604ValGlu: 4.604 ± 0.044	2.417ValPhe: 2.417 ± 0.031	6.627ValGly: 6.627 ± 0.051	1.739ValHis: 1.739 ± 0.022	3.312ValIle: 3.312 ± 0.031	1.626ValLys: 1.626 ± 0.03	9.933ValLeu: 9.933 ± 0.077	1.329ValMet: 1.329 ± 0.019	1.863ValAsn: 1.863 ± 0.03	5.184ValPro: 5.184 ± 0.056	2.077ValGln: 2.077 ± 0.023	7.01ValArg: 7.01 ± 0.046	4.578ValSer: 4.578 ± 0.043	5.981ValThr: 5.981 ± 0.055	8.573ValVal: 8.573 ± 0.066	1.146ValTrp: 1.146 ± 0.025	1.456ValTyr: 1.456 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.718TrpAla: 1.718 ± 0.027	0.123TrpCys: 0.123 ± 0.008	0.77TrpAsp: 0.77 ± 0.021	0.654TrpGlu: 0.654 ± 0.017	0.506TrpPhe: 0.506 ± 0.013	1.022TrpGly: 1.022 ± 0.025	0.402TrpHis: 0.402 ± 0.014	0.595TrpIle: 0.595 ± 0.016	0.313TrpLys: 0.313 ± 0.011	1.869TrpLeu: 1.869 ± 0.03	0.291TrpMet: 0.291 ± 0.009	0.454TrpAsn: 0.454 ± 0.014	0.899TrpPro: 0.899 ± 0.018	0.672TrpGln: 0.672 ± 0.017	1.415TrpArg: 1.415 ± 0.024	1.036TrpSer: 1.036 ± 0.025	1.059TrpThr: 1.059 ± 0.02	1.068TrpVal: 1.068 ± 0.018	0.369TrpTrp: 0.369 ± 0.013	0.321TrpTyr: 0.321 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.721TyrAla: 2.721 ± 0.032	0.158TyrCys: 0.158 ± 0.008	1.347TyrAsp: 1.347 ± 0.026	1.104TyrGlu: 1.104 ± 0.021	0.611TyrPhe: 0.611 ± 0.016	2.146TyrGly: 2.146 ± 0.031	0.39TyrHis: 0.39 ± 0.013	0.459TyrIle: 0.459 ± 0.014	0.346TyrLys: 0.346 ± 0.012	2.137TyrLeu: 2.137 ± 0.031	0.221TyrMet: 0.221 ± 0.009	0.415TyrAsn: 0.415 ± 0.013	1.095TyrPro: 1.095 ± 0.022	0.635TyrGln: 0.635 ± 0.016	1.824TyrArg: 1.824 ± 0.031	0.957TyrSer: 0.957 ± 0.018	1.141TyrThr: 1.141 ± 0.023	1.641TyrVal: 1.641 ± 0.026	0.343TyrTrp: 0.343 ± 0.014	0.424TyrTyr: 0.424 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8372 proteins (2891309 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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