Parvibaculum sedimenti

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Parvibaculaceae; Parvibaculum

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3429 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N6VMB7|A0A6N6VMB7_9RHIZ EamA family transporter OS=Parvibaculum sedimenti OX=2608632 GN=F2P47_09135 PE=4 SV=1
MM1 pKa = 7.9PYY3 pKa = 10.44SPVHH7 pKa = 4.89VRR9 pKa = 11.84RR10 pKa = 11.84SQRR13 pKa = 11.84RR14 pKa = 11.84ATKK17 pKa = 10.15LCALILPLSLWSGLAGADD35 pKa = 3.56TTISSSTTAQTTSADD50 pKa = 3.06GDD52 pKa = 3.74ITVTGTLSVGADD64 pKa = 3.23TAVTIDD70 pKa = 3.88SDD72 pKa = 4.14NSFTNEE78 pKa = 3.4GSTLAASAGGIGVSVTTATTGSIANTGTLRR108 pKa = 11.84TGTAASSDD116 pKa = 3.71TADD119 pKa = 4.33DD120 pKa = 4.27GVIGGPAIEE129 pKa = 5.0VYY131 pKa = 10.36QSLGGGISNAAGYY144 pKa = 8.09TITTYY149 pKa = 11.35GNNTIFVGTDD159 pKa = 2.76ATSPANITIGNVGTDD174 pKa = 3.17AEE176 pKa = 4.69AYY178 pKa = 9.74GIYY181 pKa = 9.79NAGYY185 pKa = 9.14IYY187 pKa = 10.08TYY189 pKa = 10.74GSNSGEE195 pKa = 3.8ATTAIRR201 pKa = 11.84VEE203 pKa = 4.11ADD205 pKa = 2.69SGYY208 pKa = 9.6TSDD211 pKa = 3.65LTGGISNAGTSSIILSRR228 pKa = 11.84AIDD231 pKa = 3.72ADD233 pKa = 3.43ATGISIGSGGIVDD246 pKa = 4.86EE247 pKa = 4.57IVNEE251 pKa = 4.26GTLEE255 pKa = 4.09ALTLGTTGGKK265 pKa = 9.58AVAISVEE272 pKa = 4.09AGGTLNGITNSGTIEE287 pKa = 3.98ASASTVGDD295 pKa = 3.24AVAIVDD301 pKa = 3.97ASGTLSSIVNSGTIEE316 pKa = 4.62AIANDD321 pKa = 4.26GAATAIDD328 pKa = 3.95VSTSASAFTLTNSGTITGAIYY349 pKa = 10.45LGSGASTITSDD360 pKa = 5.01DD361 pKa = 3.33GTITGNIDD369 pKa = 3.02IASGGTLDD377 pKa = 3.78MSLSGGAEE385 pKa = 3.8FTGGTNTGATGTLDD399 pKa = 3.48VQDD402 pKa = 3.51ATFRR406 pKa = 11.84SAGSGYY412 pKa = 10.61YY413 pKa = 9.12ATTAAFASDD422 pKa = 3.93SIFAVTYY429 pKa = 10.25DD430 pKa = 3.45AVANTSALLTTTGTTSFAAGATVDD454 pKa = 3.79VTFSSYY460 pKa = 11.17LSTSEE465 pKa = 4.28TINLVDD471 pKa = 5.05AGTLSLSAGGVDD483 pKa = 4.2DD484 pKa = 5.22LLIGGVSAGYY494 pKa = 9.84NAVLSTSGNEE504 pKa = 4.22LYY506 pKa = 9.08ITLARR511 pKa = 11.84KK512 pKa = 7.1TAAEE516 pKa = 4.1LGITGNAATIYY527 pKa = 10.31NVAPTAFALDD537 pKa = 4.18DD538 pKa = 3.92EE539 pKa = 5.27FGAAVGNLSTVAAIQKK555 pKa = 9.76LYY557 pKa = 11.02KK558 pKa = 10.41DD559 pKa = 4.67LLPDD563 pKa = 3.72LSGARR568 pKa = 11.84EE569 pKa = 3.9EE570 pKa = 4.19QALRR574 pKa = 11.84VQDD577 pKa = 3.44ISSGIVSSRR586 pKa = 11.84LDD588 pKa = 3.61LLRR591 pKa = 11.84SDD593 pKa = 5.03DD594 pKa = 4.4QDD596 pKa = 3.68GQTTGYY602 pKa = 9.66RR603 pKa = 11.84RR604 pKa = 11.84RR605 pKa = 11.84RR606 pKa = 11.84NTGLWAQEE614 pKa = 3.83AVSAEE619 pKa = 4.11SGAGGEE625 pKa = 4.54KK626 pKa = 10.02SASYY630 pKa = 11.02DD631 pKa = 3.22GTLYY635 pKa = 11.26ALAVGYY641 pKa = 7.48DD642 pKa = 3.38TRR644 pKa = 11.84DD645 pKa = 2.95GDD647 pKa = 4.11GDD649 pKa = 3.67VSGASFTYY657 pKa = 10.75AAMQYY662 pKa = 10.02GASDD666 pKa = 3.73PAHH669 pKa = 7.13DD670 pKa = 4.85NVNQTYY676 pKa = 10.93LLQLYY681 pKa = 9.18HH682 pKa = 6.88ALNRR686 pKa = 11.84GAFYY690 pKa = 10.15WDD692 pKa = 3.16AMGSLGLDD700 pKa = 3.11SYY702 pKa = 11.26KK703 pKa = 10.74GYY705 pKa = 10.37RR706 pKa = 11.84QVTAGDD712 pKa = 3.69VTRR715 pKa = 11.84NPYY718 pKa = 9.64ANWMGYY724 pKa = 7.31QAGLSTQVGYY734 pKa = 10.9DD735 pKa = 3.55LALGPISIRR744 pKa = 11.84PALGASYY751 pKa = 9.5TYY753 pKa = 10.69LRR755 pKa = 11.84QASYY759 pKa = 10.45TEE761 pKa = 4.06EE762 pKa = 4.93GGGDD766 pKa = 3.81GVDD769 pKa = 3.68LAINANSFQSFRR781 pKa = 11.84TTAEE785 pKa = 3.67LRR787 pKa = 11.84VSGKK791 pKa = 8.72TGGNPDD797 pKa = 3.27IDD799 pKa = 4.05PYY801 pKa = 11.29LRR803 pKa = 11.84GGITHH808 pKa = 7.22EE809 pKa = 4.63FLDD812 pKa = 4.31ATPVADD818 pKa = 3.49GHH820 pKa = 5.89FVAGGAFSVEE830 pKa = 3.68GDD832 pKa = 4.33ALDD835 pKa = 4.34KK836 pKa = 10.84DD837 pKa = 3.83IPFAGLGLGIGSGYY851 pKa = 10.93ARR853 pKa = 11.84LNFDD857 pKa = 3.23YY858 pKa = 10.53TGQFGDD864 pKa = 4.43KK865 pKa = 9.1LTSHH869 pKa = 6.46QATATFVMKK878 pKa = 10.16FF879 pKa = 3.22

Molecular weight: 88.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N6VGA5|A0A6N6VGA5_9RHIZ Putative sulfate exporter family transporter OS=Parvibaculum sedimenti OX=2608632 GN=F2P47_12745 PE=3 SV=1
MM1 pKa = 7.49AFVRR5 pKa = 11.84FAGRR9 pKa = 11.84KK10 pKa = 4.7VTVPGSPWMRR20 pKa = 11.84KK21 pKa = 5.64TAGAALIGGGALGFLPVLGFWMIPLGVVVLSVDD54 pKa = 3.09SHH56 pKa = 5.34RR57 pKa = 11.84VRR59 pKa = 11.84RR60 pKa = 11.84VRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84TEE66 pKa = 3.65VWWGRR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84SS75 pKa = 3.07

Molecular weight: 8.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3429

0

3429

1103409

25

2854

321.8

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.149 ± 0.063

0.844 ± 0.013

5.644 ± 0.028

6.183 ± 0.037

3.81 ± 0.027

8.503 ± 0.04

2.162 ± 0.016

5.333 ± 0.027

3.896 ± 0.039

9.909 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.018

2.714 ± 0.024

5.075 ± 0.026

2.838 ± 0.023

6.956 ± 0.049

5.308 ± 0.031

5.186 ± 0.029

7.27 ± 0.035

1.315 ± 0.015

2.414 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski