Amino acid dipepetide frequency for Pseudomonas phage Dolphis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.53AlaAla: 16.53 ± 1.699
1.163AlaCys: 1.163 ± 0.265
6.661AlaAsp: 6.661 ± 0.489
8.071AlaGlu: 8.071 ± 0.527
3.102AlaPhe: 3.102 ± 0.341
9.27AlaGly: 9.27 ± 0.812
1.48AlaHis: 1.48 ± 0.236
5.78AlaIle: 5.78 ± 0.405
6.344AlaLys: 6.344 ± 0.611
8.318AlaLeu: 8.318 ± 0.548
3.278AlaMet: 3.278 ± 0.357
3.736AlaAsn: 3.736 ± 0.33
5.075AlaPro: 5.075 ± 0.626
5.498AlaGln: 5.498 ± 0.475
5.146AlaArg: 5.146 ± 0.431
6.802AlaSer: 6.802 ± 0.749
5.075AlaThr: 5.075 ± 0.415
7.366AlaVal: 7.366 ± 0.638
1.939AlaTrp: 1.939 ± 0.3
2.82AlaTyr: 2.82 ± 0.312
0.0AlaXaa: 0.0 ± 0.0
Cys
0.846CysAla: 0.846 ± 0.163
0.176CysCys: 0.176 ± 0.078
0.423CysAsp: 0.423 ± 0.14
0.423CysGlu: 0.423 ± 0.139
0.317CysPhe: 0.317 ± 0.129
1.375CysGly: 1.375 ± 0.234
0.317CysHis: 0.317 ± 0.094
0.423CysIle: 0.423 ± 0.129
0.705CysLys: 0.705 ± 0.173
0.811CysLeu: 0.811 ± 0.177
0.317CysMet: 0.317 ± 0.126
0.493CysAsn: 0.493 ± 0.137
0.529CysPro: 0.529 ± 0.165
0.529CysGln: 0.529 ± 0.126
0.987CysArg: 0.987 ± 0.16
0.775CysSer: 0.775 ± 0.171
0.564CysThr: 0.564 ± 0.145
1.022CysVal: 1.022 ± 0.155
0.388CysTrp: 0.388 ± 0.126
0.317CysTyr: 0.317 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
7.648AspAla: 7.648 ± 0.559
0.74AspCys: 0.74 ± 0.166
3.666AspAsp: 3.666 ± 0.378
4.652AspGlu: 4.652 ± 0.471
1.903AspPhe: 1.903 ± 0.266
5.78AspGly: 5.78 ± 0.454
1.234AspHis: 1.234 ± 0.254
2.573AspIle: 2.573 ± 0.353
2.749AspLys: 2.749 ± 0.359
5.04AspLeu: 5.04 ± 0.457
1.445AspMet: 1.445 ± 0.183
2.009AspAsn: 2.009 ± 0.253
2.608AspPro: 2.608 ± 0.387
2.22AspGln: 2.22 ± 0.244
3.525AspArg: 3.525 ± 0.39
2.714AspSer: 2.714 ± 0.341
2.679AspThr: 2.679 ± 0.259
3.525AspVal: 3.525 ± 0.33
0.916AspTrp: 0.916 ± 0.164
1.833AspTyr: 1.833 ± 0.226
0.0AspXaa: 0.0 ± 0.0
Glu
7.719GluAla: 7.719 ± 0.515
0.634GluCys: 0.634 ± 0.152
2.89GluAsp: 2.89 ± 0.382
3.842GluGlu: 3.842 ± 0.468
2.115GluPhe: 2.115 ± 0.301
4.864GluGly: 4.864 ± 0.428
1.586GluHis: 1.586 ± 0.296
3.066GluIle: 3.066 ± 0.312
3.207GluLys: 3.207 ± 0.382
6.732GluLeu: 6.732 ± 0.567
1.41GluMet: 1.41 ± 0.228
1.198GluAsn: 1.198 ± 0.192
3.313GluPro: 3.313 ± 0.377
3.983GluGln: 3.983 ± 0.493
4.829GluArg: 4.829 ± 0.425
2.855GluSer: 2.855 ± 0.307
2.573GluThr: 2.573 ± 0.307
3.701GluVal: 3.701 ± 0.287
1.41GluTrp: 1.41 ± 0.231
1.657GluTyr: 1.657 ± 0.209
0.0GluXaa: 0.0 ± 0.0
Phe
3.243PheAla: 3.243 ± 0.334
0.458PheCys: 0.458 ± 0.122
2.397PheAsp: 2.397 ± 0.33
2.256PheGlu: 2.256 ± 0.269
0.423PhePhe: 0.423 ± 0.132
2.961PheGly: 2.961 ± 0.319
0.705PheHis: 0.705 ± 0.17
0.987PheIle: 0.987 ± 0.211
1.269PheLys: 1.269 ± 0.185
2.044PheLeu: 2.044 ± 0.289
1.022PheMet: 1.022 ± 0.171
1.41PheAsn: 1.41 ± 0.173
1.445PhePro: 1.445 ± 0.243
0.952PheGln: 0.952 ± 0.202
2.115PheArg: 2.115 ± 0.283
1.939PheSer: 1.939 ± 0.249
2.361PheThr: 2.361 ± 0.316
2.08PheVal: 2.08 ± 0.303
0.317PheTrp: 0.317 ± 0.112
0.846PheTyr: 0.846 ± 0.188
0.0PheXaa: 0.0 ± 0.0
Gly
8.248GlyAla: 8.248 ± 0.599
0.987GlyCys: 0.987 ± 0.198
4.582GlyAsp: 4.582 ± 0.516
4.864GlyGlu: 4.864 ± 0.271
2.573GlyPhe: 2.573 ± 0.299
7.296GlyGly: 7.296 ± 0.975
1.657GlyHis: 1.657 ± 0.24
3.948GlyIle: 3.948 ± 0.407
4.265GlyLys: 4.265 ± 0.768
6.168GlyLeu: 6.168 ± 0.521
2.22GlyMet: 2.22 ± 0.313
3.56GlyAsn: 3.56 ± 0.415
2.467GlyPro: 2.467 ± 0.344
3.525GlyGln: 3.525 ± 0.342
5.287GlyArg: 5.287 ± 0.419
5.04GlySer: 5.04 ± 0.497
4.899GlyThr: 4.899 ± 0.482
5.816GlyVal: 5.816 ± 0.51
1.868GlyTrp: 1.868 ± 0.325
2.714GlyTyr: 2.714 ± 0.307
0.0GlyXaa: 0.0 ± 0.0
His
2.538HisAla: 2.538 ± 0.443
0.247HisCys: 0.247 ± 0.086
1.234HisAsp: 1.234 ± 0.208
1.445HisGlu: 1.445 ± 0.284
0.846HisPhe: 0.846 ± 0.201
1.939HisGly: 1.939 ± 0.229
0.881HisHis: 0.881 ± 0.215
0.458HisIle: 0.458 ± 0.141
0.881HisLys: 0.881 ± 0.168
1.868HisLeu: 1.868 ± 0.284
0.599HisMet: 0.599 ± 0.141
0.634HisAsn: 0.634 ± 0.135
1.727HisPro: 1.727 ± 0.297
1.128HisGln: 1.128 ± 0.198
1.198HisArg: 1.198 ± 0.243
0.952HisSer: 0.952 ± 0.215
0.846HisThr: 0.846 ± 0.16
1.516HisVal: 1.516 ± 0.216
0.141HisTrp: 0.141 ± 0.077
0.564HisTyr: 0.564 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
5.146IleAla: 5.146 ± 0.378
0.634IleCys: 0.634 ± 0.153
3.736IleAsp: 3.736 ± 0.414
3.489IleGlu: 3.489 ± 0.321
0.634IlePhe: 0.634 ± 0.169
3.313IleGly: 3.313 ± 0.369
1.128IleHis: 1.128 ± 0.227
1.939IleIle: 1.939 ± 0.267
3.207IleLys: 3.207 ± 0.336
2.679IleLeu: 2.679 ± 0.321
0.846IleMet: 0.846 ± 0.156
2.115IleAsn: 2.115 ± 0.242
2.538IlePro: 2.538 ± 0.269
1.833IleGln: 1.833 ± 0.261
2.925IleArg: 2.925 ± 0.301
2.608IleSer: 2.608 ± 0.337
2.538IleThr: 2.538 ± 0.282
3.137IleVal: 3.137 ± 0.383
0.634IleTrp: 0.634 ± 0.153
1.304IleTyr: 1.304 ± 0.173
0.0IleXaa: 0.0 ± 0.0
Lys
7.507LysAla: 7.507 ± 0.676
0.423LysCys: 0.423 ± 0.118
2.432LysAsp: 2.432 ± 0.267
2.185LysGlu: 2.185 ± 0.264
1.727LysPhe: 1.727 ± 0.234
3.63LysGly: 3.63 ± 0.359
1.339LysHis: 1.339 ± 0.246
2.08LysIle: 2.08 ± 0.316
2.573LysLys: 2.573 ± 0.399
3.912LysLeu: 3.912 ± 0.399
0.952LysMet: 0.952 ± 0.205
1.621LysAsn: 1.621 ± 0.239
3.278LysPro: 3.278 ± 0.38
2.22LysGln: 2.22 ± 0.368
3.278LysArg: 3.278 ± 0.399
2.291LysSer: 2.291 ± 0.309
3.137LysThr: 3.137 ± 0.341
3.243LysVal: 3.243 ± 0.409
0.846LysTrp: 0.846 ± 0.145
0.881LysTyr: 0.881 ± 0.153
0.0LysXaa: 0.0 ± 0.0
Leu
8.671LeuAla: 8.671 ± 0.512
0.952LeuCys: 0.952 ± 0.18
3.666LeuAsp: 3.666 ± 0.374
5.498LeuGlu: 5.498 ± 0.564
1.762LeuPhe: 1.762 ± 0.234
5.287LeuGly: 5.287 ± 0.435
1.903LeuHis: 1.903 ± 0.312
3.701LeuIle: 3.701 ± 0.325
4.053LeuLys: 4.053 ± 0.36
5.534LeuLeu: 5.534 ± 0.549
1.868LeuMet: 1.868 ± 0.275
3.313LeuAsn: 3.313 ± 0.391
4.758LeuPro: 4.758 ± 0.441
2.643LeuGln: 2.643 ± 0.256
5.322LeuArg: 5.322 ± 0.414
4.758LeuSer: 4.758 ± 0.382
4.511LeuThr: 4.511 ± 0.364
6.168LeuVal: 6.168 ± 0.475
0.881LeuTrp: 0.881 ± 0.195
1.375LeuTyr: 1.375 ± 0.214
0.0LeuXaa: 0.0 ± 0.0
Met
3.066MetAla: 3.066 ± 0.31
0.282MetCys: 0.282 ± 0.097
0.987MetAsp: 0.987 ± 0.197
1.234MetGlu: 1.234 ± 0.243
0.634MetPhe: 0.634 ± 0.173
1.621MetGly: 1.621 ± 0.307
0.564MetHis: 0.564 ± 0.149
1.093MetIle: 1.093 ± 0.185
1.234MetLys: 1.234 ± 0.188
1.621MetLeu: 1.621 ± 0.27
0.423MetMet: 0.423 ± 0.153
0.811MetAsn: 0.811 ± 0.192
1.903MetPro: 1.903 ± 0.311
1.022MetGln: 1.022 ± 0.177
1.974MetArg: 1.974 ± 0.343
2.502MetSer: 2.502 ± 0.302
1.445MetThr: 1.445 ± 0.287
1.551MetVal: 1.551 ± 0.278
0.493MetTrp: 0.493 ± 0.147
0.564MetTyr: 0.564 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
3.701AsnAla: 3.701 ± 0.424
0.599AsnCys: 0.599 ± 0.187
1.657AsnAsp: 1.657 ± 0.21
2.256AsnGlu: 2.256 ± 0.247
1.198AsnPhe: 1.198 ± 0.242
3.771AsnGly: 3.771 ± 0.357
0.67AsnHis: 0.67 ± 0.161
1.269AsnIle: 1.269 ± 0.258
1.445AsnLys: 1.445 ± 0.212
2.115AsnLeu: 2.115 ± 0.277
0.881AsnMet: 0.881 ± 0.21
1.41AsnAsn: 1.41 ± 0.2
2.256AsnPro: 2.256 ± 0.315
1.727AsnGln: 1.727 ± 0.241
2.256AsnArg: 2.256 ± 0.328
2.115AsnSer: 2.115 ± 0.338
2.432AsnThr: 2.432 ± 0.415
2.538AsnVal: 2.538 ± 0.328
0.811AsnTrp: 0.811 ± 0.145
0.775AsnTyr: 0.775 ± 0.19
0.0AsnXaa: 0.0 ± 0.0
Pro
6.802ProAla: 6.802 ± 0.746
0.423ProCys: 0.423 ± 0.135
3.525ProAsp: 3.525 ± 0.453
3.983ProGlu: 3.983 ± 0.344
1.868ProPhe: 1.868 ± 0.226
4.441ProGly: 4.441 ± 0.502
1.198ProHis: 1.198 ± 0.181
2.397ProIle: 2.397 ± 0.312
2.749ProLys: 2.749 ± 0.423
2.82ProLeu: 2.82 ± 0.277
1.551ProMet: 1.551 ± 0.249
1.621ProAsn: 1.621 ± 0.261
2.855ProPro: 2.855 ± 0.369
1.833ProGln: 1.833 ± 0.237
2.08ProArg: 2.08 ± 0.302
3.666ProSer: 3.666 ± 0.353
2.749ProThr: 2.749 ± 0.294
3.842ProVal: 3.842 ± 0.447
0.388ProTrp: 0.388 ± 0.135
1.304ProTyr: 1.304 ± 0.205
0.0ProXaa: 0.0 ± 0.0
Gln
5.181GlnAla: 5.181 ± 0.43
0.388GlnCys: 0.388 ± 0.12
1.939GlnAsp: 1.939 ± 0.263
2.573GlnGlu: 2.573 ± 0.36
1.868GlnPhe: 1.868 ± 0.245
3.525GlnGly: 3.525 ± 0.352
1.022GlnHis: 1.022 ± 0.217
2.291GlnIle: 2.291 ± 0.329
1.551GlnLys: 1.551 ± 0.238
3.525GlnLeu: 3.525 ± 0.349
0.952GlnMet: 0.952 ± 0.176
1.234GlnAsn: 1.234 ± 0.214
1.868GlnPro: 1.868 ± 0.272
3.066GlnGln: 3.066 ± 0.626
3.525GlnArg: 3.525 ± 0.41
2.185GlnSer: 2.185 ± 0.221
2.256GlnThr: 2.256 ± 0.307
4.018GlnVal: 4.018 ± 0.404
0.634GlnTrp: 0.634 ± 0.12
1.269GlnTyr: 1.269 ± 0.264
0.0GlnXaa: 0.0 ± 0.0
Arg
5.287ArgAla: 5.287 ± 0.49
0.634ArgCys: 0.634 ± 0.158
4.124ArgAsp: 4.124 ± 0.41
3.842ArgGlu: 3.842 ± 0.398
2.749ArgPhe: 2.749 ± 0.339
4.053ArgGly: 4.053 ± 0.411
1.48ArgHis: 1.48 ± 0.255
2.996ArgIle: 2.996 ± 0.305
3.207ArgLys: 3.207 ± 0.351
5.463ArgLeu: 5.463 ± 0.555
1.974ArgMet: 1.974 ± 0.3
2.185ArgAsn: 2.185 ± 0.283
3.348ArgPro: 3.348 ± 0.446
3.172ArgGln: 3.172 ± 0.382
4.652ArgArg: 4.652 ± 0.53
3.031ArgSer: 3.031 ± 0.335
3.137ArgThr: 3.137 ± 0.362
4.3ArgVal: 4.3 ± 0.413
1.657ArgTrp: 1.657 ± 0.243
1.621ArgTyr: 1.621 ± 0.201
0.0ArgXaa: 0.0 ± 0.0
Ser
6.133SerAla: 6.133 ± 0.574
0.987SerCys: 0.987 ± 0.21
3.807SerAsp: 3.807 ± 0.377
3.102SerGlu: 3.102 ± 0.301
1.48SerPhe: 1.48 ± 0.231
5.498SerGly: 5.498 ± 0.427
0.952SerHis: 0.952 ± 0.217
2.855SerIle: 2.855 ± 0.26
2.608SerLys: 2.608 ± 0.336
4.582SerLeu: 4.582 ± 0.48
1.48SerMet: 1.48 ± 0.241
2.573SerAsn: 2.573 ± 0.289
2.961SerPro: 2.961 ± 0.348
2.432SerGln: 2.432 ± 0.271
2.855SerArg: 2.855 ± 0.421
3.348SerSer: 3.348 ± 0.333
2.996SerThr: 2.996 ± 0.297
4.124SerVal: 4.124 ± 0.375
1.163SerTrp: 1.163 ± 0.202
1.375SerTyr: 1.375 ± 0.252
0.0SerXaa: 0.0 ± 0.0
Thr
4.652ThrAla: 4.652 ± 0.365
0.564ThrCys: 0.564 ± 0.145
3.348ThrAsp: 3.348 ± 0.329
2.608ThrGlu: 2.608 ± 0.306
2.044ThrPhe: 2.044 ± 0.254
5.111ThrGly: 5.111 ± 0.376
0.705ThrHis: 0.705 ± 0.153
2.961ThrIle: 2.961 ± 0.348
2.361ThrLys: 2.361 ± 0.31
3.983ThrLeu: 3.983 ± 0.412
1.516ThrMet: 1.516 ± 0.237
1.939ThrAsn: 1.939 ± 0.215
3.454ThrPro: 3.454 ± 0.357
2.256ThrGln: 2.256 ± 0.292
2.996ThrArg: 2.996 ± 0.339
3.701ThrSer: 3.701 ± 0.371
2.608ThrThr: 2.608 ± 0.317
4.018ThrVal: 4.018 ± 0.454
1.057ThrTrp: 1.057 ± 0.23
1.974ThrTyr: 1.974 ± 0.259
0.0ThrXaa: 0.0 ± 0.0
Val
6.626ValAla: 6.626 ± 0.425
0.811ValCys: 0.811 ± 0.176
5.146ValAsp: 5.146 ± 0.417
5.252ValGlu: 5.252 ± 0.442
2.538ValPhe: 2.538 ± 0.307
4.441ValGly: 4.441 ± 0.44
1.586ValHis: 1.586 ± 0.229
3.56ValIle: 3.56 ± 0.381
3.454ValLys: 3.454 ± 0.41
5.357ValLeu: 5.357 ± 0.483
1.727ValMet: 1.727 ± 0.28
2.608ValAsn: 2.608 ± 0.361
3.877ValPro: 3.877 ± 0.505
2.89ValGln: 2.89 ± 0.394
4.582ValArg: 4.582 ± 0.45
3.525ValSer: 3.525 ± 0.408
4.582ValThr: 4.582 ± 0.468
5.04ValVal: 5.04 ± 0.492
0.74ValTrp: 0.74 ± 0.159
1.48ValTyr: 1.48 ± 0.247
0.0ValXaa: 0.0 ± 0.0
Trp
1.269TrpAla: 1.269 ± 0.242
0.176TrpCys: 0.176 ± 0.074
1.48TrpAsp: 1.48 ± 0.2
0.916TrpGlu: 0.916 ± 0.202
0.705TrpPhe: 0.705 ± 0.213
1.128TrpGly: 1.128 ± 0.174
0.423TrpHis: 0.423 ± 0.128
0.599TrpIle: 0.599 ± 0.143
0.775TrpLys: 0.775 ± 0.167
1.727TrpLeu: 1.727 ± 0.226
0.176TrpMet: 0.176 ± 0.073
0.67TrpAsn: 0.67 ± 0.152
0.881TrpPro: 0.881 ± 0.161
0.705TrpGln: 0.705 ± 0.148
1.48TrpArg: 1.48 ± 0.255
1.269TrpSer: 1.269 ± 0.167
0.916TrpThr: 0.916 ± 0.192
0.916TrpVal: 0.916 ± 0.169
0.176TrpTrp: 0.176 ± 0.075
0.388TrpTyr: 0.388 ± 0.1
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.643TyrAla: 2.643 ± 0.283
0.458TyrCys: 0.458 ± 0.149
1.762TyrAsp: 1.762 ± 0.19
1.093TyrGlu: 1.093 ± 0.221
0.74TyrPhe: 0.74 ± 0.16
2.679TyrGly: 2.679 ± 0.33
0.811TyrHis: 0.811 ± 0.191
1.41TyrIle: 1.41 ± 0.204
0.916TyrLys: 0.916 ± 0.177
2.326TyrLeu: 2.326 ± 0.261
0.352TyrMet: 0.352 ± 0.117
0.74TyrAsn: 0.74 ± 0.155
0.987TyrPro: 0.987 ± 0.182
1.234TyrGln: 1.234 ± 0.19
1.903TyrArg: 1.903 ± 0.324
1.234TyrSer: 1.234 ± 0.248
1.551TyrThr: 1.551 ± 0.211
1.903TyrVal: 1.903 ± 0.303
0.317TyrTrp: 0.317 ± 0.091
0.74TyrTyr: 0.74 ± 0.129
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 124 proteins (28373 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski