Amino acid dipepetide frequency for Mycobacterium phage Trixie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.781AlaAla: 10.781 ± 0.991
0.545AlaCys: 0.545 ± 0.18
5.27AlaAsp: 5.27 ± 0.619
8.056AlaGlu: 8.056 ± 0.876
3.513AlaPhe: 3.513 ± 0.506
8.964AlaGly: 8.964 ± 0.842
1.211AlaHis: 1.211 ± 0.278
4.422AlaIle: 4.422 ± 0.475
4.846AlaLys: 4.846 ± 0.616
8.722AlaLeu: 8.722 ± 0.899
2.604AlaMet: 2.604 ± 0.444
3.331AlaAsn: 3.331 ± 0.463
4.906AlaPro: 4.906 ± 0.739
4.24AlaGln: 4.24 ± 0.625
6.602AlaArg: 6.602 ± 0.743
4.179AlaSer: 4.179 ± 0.575
6.057AlaThr: 6.057 ± 0.697
7.087AlaVal: 7.087 ± 0.674
1.817AlaTrp: 1.817 ± 0.358
2.847AlaTyr: 2.847 ± 0.527
0.0AlaXaa: 0.0 ± 0.0
Cys
0.606CysAla: 0.606 ± 0.187
0.0CysCys: 0.0 ± 0.0
0.545CysAsp: 0.545 ± 0.19
0.545CysGlu: 0.545 ± 0.203
0.424CysPhe: 0.424 ± 0.157
0.848CysGly: 0.848 ± 0.261
0.242CysHis: 0.242 ± 0.134
0.303CysIle: 0.303 ± 0.144
0.303CysLys: 0.303 ± 0.141
0.485CysLeu: 0.485 ± 0.168
0.242CysMet: 0.242 ± 0.128
0.363CysAsn: 0.363 ± 0.134
0.485CysPro: 0.485 ± 0.208
0.182CysGln: 0.182 ± 0.1
0.545CysArg: 0.545 ± 0.207
0.485CysSer: 0.485 ± 0.173
0.666CysThr: 0.666 ± 0.186
0.666CysVal: 0.666 ± 0.178
0.363CysTrp: 0.363 ± 0.17
0.485CysTyr: 0.485 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
6.239AspAla: 6.239 ± 0.591
0.666AspCys: 0.666 ± 0.252
4.482AspAsp: 4.482 ± 0.63
4.3AspGlu: 4.3 ± 0.626
2.483AspPhe: 2.483 ± 0.498
5.391AspGly: 5.391 ± 0.559
1.454AspHis: 1.454 ± 0.318
3.574AspIle: 3.574 ± 0.414
2.544AspLys: 2.544 ± 0.432
5.088AspLeu: 5.088 ± 0.613
1.878AspMet: 1.878 ± 0.343
1.151AspAsn: 1.151 ± 0.226
4.422AspPro: 4.422 ± 0.677
1.938AspGln: 1.938 ± 0.296
3.089AspArg: 3.089 ± 0.519
2.423AspSer: 2.423 ± 0.359
3.331AspThr: 3.331 ± 0.557
4.482AspVal: 4.482 ± 0.551
1.272AspTrp: 1.272 ± 0.339
2.423AspTyr: 2.423 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
7.813GluAla: 7.813 ± 0.974
0.242GluCys: 0.242 ± 0.168
4.846GluAsp: 4.846 ± 0.628
4.967GluGlu: 4.967 ± 0.656
3.21GluPhe: 3.21 ± 0.456
5.391GluGly: 5.391 ± 0.568
1.514GluHis: 1.514 ± 0.288
3.028GluIle: 3.028 ± 0.42
2.726GluLys: 2.726 ± 0.41
7.692GluLeu: 7.692 ± 0.864
2.12GluMet: 2.12 ± 0.351
2.059GluAsn: 2.059 ± 0.32
2.968GluPro: 2.968 ± 0.544
2.059GluGln: 2.059 ± 0.273
4.422GluArg: 4.422 ± 0.456
2.726GluSer: 2.726 ± 0.419
3.392GluThr: 3.392 ± 0.377
4.664GluVal: 4.664 ± 0.51
1.393GluTrp: 1.393 ± 0.234
1.514GluTyr: 1.514 ± 0.266
0.0GluXaa: 0.0 ± 0.0
Phe
3.089PheAla: 3.089 ± 0.377
0.242PheCys: 0.242 ± 0.117
2.786PheAsp: 2.786 ± 0.502
3.21PheGlu: 3.21 ± 0.44
1.03PhePhe: 1.03 ± 0.249
3.452PheGly: 3.452 ± 0.512
0.909PheHis: 0.909 ± 0.23
1.575PheIle: 1.575 ± 0.247
1.757PheLys: 1.757 ± 0.331
2.726PheLeu: 2.726 ± 0.503
0.424PheMet: 0.424 ± 0.147
1.757PheAsn: 1.757 ± 0.337
1.999PhePro: 1.999 ± 0.335
1.151PheGln: 1.151 ± 0.274
1.878PheArg: 1.878 ± 0.276
2.12PheSer: 2.12 ± 0.329
2.059PheThr: 2.059 ± 0.278
2.483PheVal: 2.483 ± 0.343
0.485PheTrp: 0.485 ± 0.163
0.787PheTyr: 0.787 ± 0.193
0.0PheXaa: 0.0 ± 0.0
Gly
7.389GlyAla: 7.389 ± 0.821
0.909GlyCys: 0.909 ± 0.223
6.481GlyAsp: 6.481 ± 0.742
5.209GlyGlu: 5.209 ± 0.524
3.21GlyPhe: 3.21 ± 0.425
8.056GlyGly: 8.056 ± 1.452
1.635GlyHis: 1.635 ± 0.304
4.361GlyIle: 4.361 ± 0.606
3.876GlyLys: 3.876 ± 0.543
6.965GlyLeu: 6.965 ± 0.747
1.757GlyMet: 1.757 ± 0.275
3.331GlyAsn: 3.331 ± 0.481
4.603GlyPro: 4.603 ± 1.215
3.998GlyGln: 3.998 ± 0.604
3.695GlyArg: 3.695 ± 0.407
4.119GlySer: 4.119 ± 0.551
5.875GlyThr: 5.875 ± 0.571
6.481GlyVal: 6.481 ± 0.719
1.211GlyTrp: 1.211 ± 0.248
2.423GlyTyr: 2.423 ± 0.365
0.0GlyXaa: 0.0 ± 0.0
His
1.575HisAla: 1.575 ± 0.292
0.424HisCys: 0.424 ± 0.151
1.211HisAsp: 1.211 ± 0.284
1.211HisGlu: 1.211 ± 0.272
0.606HisPhe: 0.606 ± 0.199
1.757HisGly: 1.757 ± 0.38
0.485HisHis: 0.485 ± 0.164
0.969HisIle: 0.969 ± 0.257
0.848HisLys: 0.848 ± 0.223
1.514HisLeu: 1.514 ± 0.303
0.485HisMet: 0.485 ± 0.198
0.545HisAsn: 0.545 ± 0.189
1.272HisPro: 1.272 ± 0.267
0.909HisGln: 0.909 ± 0.194
1.272HisArg: 1.272 ± 0.318
0.909HisSer: 0.909 ± 0.238
0.969HisThr: 0.969 ± 0.201
1.151HisVal: 1.151 ± 0.201
0.424HisTrp: 0.424 ± 0.14
0.545HisTyr: 0.545 ± 0.251
0.0HisXaa: 0.0 ± 0.0
Ile
6.057IleAla: 6.057 ± 0.721
0.606IleCys: 0.606 ± 0.177
3.816IleAsp: 3.816 ± 0.423
4.422IleGlu: 4.422 ± 0.41
1.514IlePhe: 1.514 ± 0.307
4.482IleGly: 4.482 ± 0.636
0.787IleHis: 0.787 ± 0.194
1.938IleIle: 1.938 ± 0.305
2.483IleLys: 2.483 ± 0.43
3.695IleLeu: 3.695 ± 0.461
0.424IleMet: 0.424 ± 0.14
1.999IleAsn: 1.999 ± 0.382
3.21IlePro: 3.21 ± 0.416
1.272IleGln: 1.272 ± 0.307
2.847IleArg: 2.847 ± 0.491
2.544IleSer: 2.544 ± 0.37
2.968IleThr: 2.968 ± 0.355
2.362IleVal: 2.362 ± 0.354
0.545IleTrp: 0.545 ± 0.151
0.787IleTyr: 0.787 ± 0.168
0.0IleXaa: 0.0 ± 0.0
Lys
5.391LysAla: 5.391 ± 0.661
0.303LysCys: 0.303 ± 0.141
2.241LysAsp: 2.241 ± 0.414
2.362LysGlu: 2.362 ± 0.446
1.393LysPhe: 1.393 ± 0.288
4.058LysGly: 4.058 ± 0.84
0.666LysHis: 0.666 ± 0.202
2.12LysIle: 2.12 ± 0.373
3.089LysLys: 3.089 ± 0.549
4.361LysLeu: 4.361 ± 0.444
0.909LysMet: 0.909 ± 0.21
1.575LysAsn: 1.575 ± 0.313
2.786LysPro: 2.786 ± 0.53
1.272LysGln: 1.272 ± 0.275
3.452LysArg: 3.452 ± 0.527
1.938LysSer: 1.938 ± 0.372
2.968LysThr: 2.968 ± 0.508
3.876LysVal: 3.876 ± 0.436
0.787LysTrp: 0.787 ± 0.233
1.333LysTyr: 1.333 ± 0.229
0.0LysXaa: 0.0 ± 0.0
Leu
9.449LeuAla: 9.449 ± 0.753
0.727LeuCys: 0.727 ± 0.221
4.785LeuAsp: 4.785 ± 0.577
5.027LeuGlu: 5.027 ± 0.523
2.907LeuPhe: 2.907 ± 0.334
5.815LeuGly: 5.815 ± 0.692
1.696LeuHis: 1.696 ± 0.303
4.3LeuIle: 4.3 ± 0.551
3.452LeuLys: 3.452 ± 0.428
5.875LeuLeu: 5.875 ± 0.632
2.483LeuMet: 2.483 ± 0.41
2.302LeuAsn: 2.302 ± 0.429
4.664LeuPro: 4.664 ± 0.475
2.362LeuGln: 2.362 ± 0.486
6.239LeuArg: 6.239 ± 0.676
5.996LeuSer: 5.996 ± 0.789
5.027LeuThr: 5.027 ± 0.615
4.603LeuVal: 4.603 ± 0.71
1.817LeuTrp: 1.817 ± 0.276
2.483LeuTyr: 2.483 ± 0.478
0.0LeuXaa: 0.0 ± 0.0
Met
3.271MetAla: 3.271 ± 0.401
0.061MetCys: 0.061 ± 0.058
0.969MetAsp: 0.969 ± 0.244
1.211MetGlu: 1.211 ± 0.213
0.848MetPhe: 0.848 ± 0.199
1.514MetGly: 1.514 ± 0.313
0.424MetHis: 0.424 ± 0.164
1.03MetIle: 1.03 ± 0.253
1.514MetLys: 1.514 ± 0.288
1.635MetLeu: 1.635 ± 0.383
0.606MetMet: 0.606 ± 0.171
0.606MetAsn: 0.606 ± 0.185
1.272MetPro: 1.272 ± 0.307
1.211MetGln: 1.211 ± 0.234
1.575MetArg: 1.575 ± 0.247
1.878MetSer: 1.878 ± 0.269
2.18MetThr: 2.18 ± 0.334
1.272MetVal: 1.272 ± 0.326
0.182MetTrp: 0.182 ± 0.089
0.848MetTyr: 0.848 ± 0.222
0.0MetXaa: 0.0 ± 0.0
Asn
2.968AsnAla: 2.968 ± 0.458
0.485AsnCys: 0.485 ± 0.187
1.514AsnAsp: 1.514 ± 0.25
2.302AsnGlu: 2.302 ± 0.428
0.606AsnPhe: 0.606 ± 0.254
4.422AsnGly: 4.422 ± 0.601
1.211AsnHis: 1.211 ± 0.247
1.272AsnIle: 1.272 ± 0.258
1.09AsnLys: 1.09 ± 0.215
2.665AsnLeu: 2.665 ± 0.42
0.727AsnMet: 0.727 ± 0.184
0.727AsnAsn: 0.727 ± 0.178
2.665AsnPro: 2.665 ± 0.359
1.03AsnGln: 1.03 ± 0.288
2.059AsnArg: 2.059 ± 0.352
1.393AsnSer: 1.393 ± 0.276
1.514AsnThr: 1.514 ± 0.322
2.423AsnVal: 2.423 ± 0.365
0.787AsnTrp: 0.787 ± 0.218
1.151AsnTyr: 1.151 ± 0.252
0.0AsnXaa: 0.0 ± 0.0
Pro
5.33ProAla: 5.33 ± 0.74
0.303ProCys: 0.303 ± 0.137
3.695ProAsp: 3.695 ± 0.526
4.785ProGlu: 4.785 ± 0.501
1.817ProPhe: 1.817 ± 0.393
4.846ProGly: 4.846 ± 0.562
1.09ProHis: 1.09 ± 0.268
2.786ProIle: 2.786 ± 0.323
2.483ProLys: 2.483 ± 0.584
3.21ProLeu: 3.21 ± 0.433
1.272ProMet: 1.272 ± 0.272
2.302ProAsn: 2.302 ± 0.425
2.786ProPro: 2.786 ± 0.467
2.423ProGln: 2.423 ± 0.543
3.695ProArg: 3.695 ± 0.521
2.968ProSer: 2.968 ± 0.372
3.574ProThr: 3.574 ± 0.548
4.24ProVal: 4.24 ± 0.527
0.969ProTrp: 0.969 ± 0.318
1.514ProTyr: 1.514 ± 0.315
0.0ProXaa: 0.0 ± 0.0
Gln
3.998GlnAla: 3.998 ± 0.56
0.303GlnCys: 0.303 ± 0.121
1.272GlnAsp: 1.272 ± 0.26
1.696GlnGlu: 1.696 ± 0.288
1.575GlnPhe: 1.575 ± 0.267
3.755GlnGly: 3.755 ± 0.828
0.727GlnHis: 0.727 ± 0.209
2.423GlnIle: 2.423 ± 0.319
1.575GlnLys: 1.575 ± 0.346
3.271GlnLeu: 3.271 ± 0.646
0.969GlnMet: 0.969 ± 0.202
0.787GlnAsn: 0.787 ± 0.191
1.393GlnPro: 1.393 ± 0.27
2.12GlnGln: 2.12 ± 0.341
2.302GlnArg: 2.302 ± 0.409
1.938GlnSer: 1.938 ± 0.352
1.757GlnThr: 1.757 ± 0.35
2.544GlnVal: 2.544 ± 0.416
0.969GlnTrp: 0.969 ± 0.262
1.151GlnTyr: 1.151 ± 0.231
0.0GlnXaa: 0.0 ± 0.0
Arg
4.967ArgAla: 4.967 ± 0.798
0.848ArgCys: 0.848 ± 0.244
4.3ArgAsp: 4.3 ± 0.59
5.027ArgGlu: 5.027 ± 0.732
2.544ArgPhe: 2.544 ± 0.413
4.179ArgGly: 4.179 ± 0.645
1.211ArgHis: 1.211 ± 0.28
3.634ArgIle: 3.634 ± 0.47
3.755ArgLys: 3.755 ± 0.573
5.451ArgLeu: 5.451 ± 0.604
2.12ArgMet: 2.12 ± 0.329
2.059ArgAsn: 2.059 ± 0.33
2.604ArgPro: 2.604 ± 0.374
2.18ArgGln: 2.18 ± 0.379
4.785ArgArg: 4.785 ± 0.513
2.423ArgSer: 2.423 ± 0.381
2.726ArgThr: 2.726 ± 0.424
4.846ArgVal: 4.846 ± 0.591
1.272ArgTrp: 1.272 ± 0.316
1.878ArgTyr: 1.878 ± 0.304
0.0ArgXaa: 0.0 ± 0.0
Ser
4.543SerAla: 4.543 ± 0.502
0.485SerCys: 0.485 ± 0.154
2.968SerAsp: 2.968 ± 0.442
3.392SerGlu: 3.392 ± 0.415
2.059SerPhe: 2.059 ± 0.421
5.027SerGly: 5.027 ± 0.553
0.727SerHis: 0.727 ± 0.22
2.302SerIle: 2.302 ± 0.389
2.665SerLys: 2.665 ± 0.473
4.119SerLeu: 4.119 ± 0.561
1.211SerMet: 1.211 ± 0.261
1.575SerAsn: 1.575 ± 0.288
3.331SerPro: 3.331 ± 0.427
1.817SerGln: 1.817 ± 0.316
3.331SerArg: 3.331 ± 0.391
2.726SerSer: 2.726 ± 0.496
2.786SerThr: 2.786 ± 0.389
3.634SerVal: 3.634 ± 0.484
1.151SerTrp: 1.151 ± 0.291
1.211SerTyr: 1.211 ± 0.263
0.0SerXaa: 0.0 ± 0.0
Thr
5.875ThrAla: 5.875 ± 0.509
0.424ThrCys: 0.424 ± 0.139
3.15ThrAsp: 3.15 ± 0.57
3.452ThrGlu: 3.452 ± 0.386
2.423ThrPhe: 2.423 ± 0.415
5.33ThrGly: 5.33 ± 0.933
0.787ThrHis: 0.787 ± 0.194
2.544ThrIle: 2.544 ± 0.401
3.331ThrLys: 3.331 ± 0.481
4.603ThrLeu: 4.603 ± 0.71
1.211ThrMet: 1.211 ± 0.262
1.878ThrAsn: 1.878 ± 0.375
4.724ThrPro: 4.724 ± 0.576
2.362ThrGln: 2.362 ± 0.437
3.028ThrArg: 3.028 ± 0.461
3.089ThrSer: 3.089 ± 0.447
3.695ThrThr: 3.695 ± 0.481
4.724ThrVal: 4.724 ± 0.575
1.211ThrTrp: 1.211 ± 0.265
1.696ThrTyr: 1.696 ± 0.329
0.0ThrXaa: 0.0 ± 0.0
Val
6.299ValAla: 6.299 ± 0.805
0.666ValCys: 0.666 ± 0.21
5.209ValAsp: 5.209 ± 0.497
4.543ValGlu: 4.543 ± 0.558
2.362ValPhe: 2.362 ± 0.452
5.027ValGly: 5.027 ± 0.495
1.333ValHis: 1.333 ± 0.249
3.452ValIle: 3.452 ± 0.413
3.513ValLys: 3.513 ± 0.344
5.572ValLeu: 5.572 ± 0.556
1.514ValMet: 1.514 ± 0.31
2.907ValAsn: 2.907 ± 0.455
3.695ValPro: 3.695 ± 0.585
2.18ValGln: 2.18 ± 0.473
4.119ValArg: 4.119 ± 0.604
4.422ValSer: 4.422 ± 0.714
5.27ValThr: 5.27 ± 0.536
5.33ValVal: 5.33 ± 0.497
1.393ValTrp: 1.393 ± 0.274
2.059ValTyr: 2.059 ± 0.381
0.0ValXaa: 0.0 ± 0.0
Trp
1.757TrpAla: 1.757 ± 0.385
0.242TrpCys: 0.242 ± 0.136
1.03TrpAsp: 1.03 ± 0.205
1.09TrpGlu: 1.09 ± 0.247
0.666TrpPhe: 0.666 ± 0.201
1.333TrpGly: 1.333 ± 0.265
0.666TrpHis: 0.666 ± 0.186
1.09TrpIle: 1.09 ± 0.273
0.242TrpLys: 0.242 ± 0.136
1.817TrpLeu: 1.817 ± 0.331
0.424TrpMet: 0.424 ± 0.154
0.848TrpAsn: 0.848 ± 0.238
0.969TrpPro: 0.969 ± 0.22
0.909TrpGln: 0.909 ± 0.227
1.211TrpArg: 1.211 ± 0.249
0.909TrpSer: 0.909 ± 0.224
1.272TrpThr: 1.272 ± 0.236
1.575TrpVal: 1.575 ± 0.344
0.485TrpTrp: 0.485 ± 0.212
0.666TrpTyr: 0.666 ± 0.23
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.604TyrAla: 2.604 ± 0.333
0.303TyrCys: 0.303 ± 0.133
2.059TyrAsp: 2.059 ± 0.319
1.938TyrGlu: 1.938 ± 0.286
0.727TyrPhe: 0.727 ± 0.181
1.938TyrGly: 1.938 ± 0.384
0.303TyrHis: 0.303 ± 0.138
1.635TyrIle: 1.635 ± 0.326
0.848TyrLys: 0.848 ± 0.244
2.544TyrLeu: 2.544 ± 0.418
0.666TyrMet: 0.666 ± 0.214
0.909TyrAsn: 0.909 ± 0.204
1.333TyrPro: 1.333 ± 0.286
0.909TyrGln: 0.909 ± 0.207
2.665TyrArg: 2.665 ± 0.491
1.878TyrSer: 1.878 ± 0.316
1.454TyrThr: 1.454 ± 0.295
2.423TyrVal: 2.423 ± 0.441
0.666TyrTrp: 0.666 ± 0.238
0.606TyrTyr: 0.606 ± 0.185
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (16511 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski