Macrostomum lignano

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Platyhelminthes; Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43466 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A267ETZ5|A0A267ETZ5_9PLAT Sulfotransfer_1 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003539g1 PE=4 SV=1
MM1 pKa = 7.62APNGVSDD8 pKa = 4.2RR9 pKa = 11.84SARR12 pKa = 11.84LPRR15 pKa = 11.84LIMIRR20 pKa = 11.84ILLLVAMQATLFPGTAFAQLAWDD43 pKa = 4.14TFSPAQQTYY52 pKa = 7.7PTATVTIDD60 pKa = 3.04EE61 pKa = 4.44NMAATTTVLTITTTGATGTVTYY83 pKa = 10.26SIASSTTAAFTIGASTGVITVNDD106 pKa = 3.66ASLLDD111 pKa = 3.71RR112 pKa = 11.84EE113 pKa = 4.88AISFIDD119 pKa = 4.29LVLQAVDD126 pKa = 3.62TSPATVSTTVRR137 pKa = 11.84IDD139 pKa = 3.46LKK141 pKa = 10.77DD142 pKa = 3.65VNEE145 pKa = 4.08IAPTFSSTSTYY156 pKa = 10.81SVTISEE162 pKa = 4.55SATVGTTIQALTVTDD177 pKa = 3.69TDD179 pKa = 3.87SAATNVACTISSGDD193 pKa = 3.72PDD195 pKa = 3.64GKK197 pKa = 10.71FQFRR201 pKa = 11.84SDD203 pKa = 3.34NQTIEE208 pKa = 4.14LKK210 pKa = 10.85AAIEE214 pKa = 4.18PDD216 pKa = 3.55SPTNNSLTYY225 pKa = 9.79TLTIVCYY232 pKa = 10.79DD233 pKa = 3.69NFNGVYY239 pKa = 10.14SGGSLASTGTVSVSVTPVNDD259 pKa = 3.98NSPTWGTFTPALATPITTIDD279 pKa = 3.5EE280 pKa = 4.24NLASGTIFTAAASDD294 pKa = 3.48VDD296 pKa = 4.09YY297 pKa = 8.96GTDD300 pKa = 3.47GVVTFSFSTVKK311 pKa = 10.6FNTGADD317 pKa = 3.29ASALFTIGSSSGVVSRR333 pKa = 11.84TAATTPALDD342 pKa = 3.46RR343 pKa = 11.84EE344 pKa = 4.89TYY346 pKa = 9.25SHH348 pKa = 6.83VEE350 pKa = 4.04FVVLAMDD357 pKa = 4.43SPTVTADD364 pKa = 3.5RR365 pKa = 11.84LTITQTVQVNLKK377 pKa = 9.81DD378 pKa = 3.91VNEE381 pKa = 4.15FTPVFDD387 pKa = 5.16PSTGLYY393 pKa = 9.52NVSFSEE399 pKa = 4.21TAPAGTLVLNVSVSDD414 pKa = 3.9PDD416 pKa = 3.99FSRR419 pKa = 11.84TAISCTITSGNTLDD433 pKa = 3.9RR434 pKa = 11.84FQMRR438 pKa = 11.84ADD440 pKa = 3.71GTSLEE445 pKa = 4.92LKK447 pKa = 8.65TQIEE451 pKa = 4.42PDD453 pKa = 3.93SPTSDD458 pKa = 4.1PINYY462 pKa = 9.36VLTLSCADD470 pKa = 3.66NTAGTYY476 pKa = 10.31SGGQKK481 pKa = 10.3SSTGTVTVWVEE492 pKa = 3.66YY493 pKa = 11.02NNDD496 pKa = 3.57YY497 pKa = 11.24SPVWSTFSPAWTSATTPISTILEE520 pKa = 4.01NLAVNQTVFTASATDD535 pKa = 4.34DD536 pKa = 3.9DD537 pKa = 5.62AGDD540 pKa = 4.65DD541 pKa = 3.93GLVVYY546 pKa = 10.13SISGAVAEE554 pKa = 4.69NGSSVMEE561 pKa = 4.03AFAINGSSGAVSIANSTVLDD581 pKa = 3.81RR582 pKa = 11.84EE583 pKa = 4.82GYY585 pKa = 9.46SYY587 pKa = 12.0VDD589 pKa = 3.35VTVEE593 pKa = 4.26ARR595 pKa = 11.84DD596 pKa = 3.9SPASGTTYY604 pKa = 10.46TITQTVRR611 pKa = 11.84IGISDD616 pKa = 3.83YY617 pKa = 11.79NEE619 pKa = 3.95FPPAFTPSTGLYY631 pKa = 8.94TPAIPEE637 pKa = 4.06TTTAGTAVQTIVATDD652 pKa = 3.64PDD654 pKa = 4.28YY655 pKa = 11.85SNTAVVCAIAAGNSLNRR672 pKa = 11.84FAINTADD679 pKa = 3.58KK680 pKa = 10.83KK681 pKa = 10.75LVEE684 pKa = 4.42TASVIQPDD692 pKa = 4.8KK693 pKa = 9.98PTLDD697 pKa = 3.3PTIYY701 pKa = 10.86LLTVHH706 pKa = 6.63CADD709 pKa = 4.41MNNGTLGTPVLTSTAYY725 pKa = 8.23ITVNVTLVNDD735 pKa = 3.79YY736 pKa = 11.47DD737 pKa = 3.76PTITLNSTTMVIPEE751 pKa = 4.35DD752 pKa = 4.05TPVSSFIVSVTADD765 pKa = 3.62DD766 pKa = 4.94LDD768 pKa = 4.08YY769 pKa = 11.75GNDD772 pKa = 3.55GTFTISLSPTTPFALTGDD790 pKa = 3.83RR791 pKa = 11.84TKK793 pKa = 11.31LLLTEE798 pKa = 4.29ALDD801 pKa = 4.37RR802 pKa = 11.84EE803 pKa = 4.73TKK805 pKa = 10.12DD806 pKa = 3.04SYY808 pKa = 10.22VIEE811 pKa = 4.26MRR813 pKa = 11.84ALDD816 pKa = 3.63QGSPARR822 pKa = 11.84SSTTTLTVSITDD834 pKa = 3.62VNDD837 pKa = 3.28NTPVCSPNTYY847 pKa = 9.75NVSVTADD854 pKa = 3.18SAATLVASIPCTDD867 pKa = 4.48ADD869 pKa = 4.88LGDD872 pKa = 4.12NQLLAYY878 pKa = 10.13SFLSGDD884 pKa = 3.55TRR886 pKa = 11.84NLTLSSTTGALTTTFLTYY904 pKa = 10.88DD905 pKa = 3.13SAAPSKK911 pKa = 9.64TLVITVRR918 pKa = 11.84DD919 pKa = 3.69SATPASAQRR928 pKa = 11.84TATVTVTVHH937 pKa = 5.35VLPRR941 pKa = 11.84NLGPPVWGTFNPPWISATTAINSVSEE967 pKa = 4.06NADD970 pKa = 2.94EE971 pKa = 4.6GFAVFTAVAIDD982 pKa = 4.18YY983 pKa = 10.81DD984 pKa = 4.28SGPDD988 pKa = 3.54GQVIYY993 pKa = 10.93SLASVTGTDD1002 pKa = 3.25GTTSVDD1008 pKa = 3.27LSSVFNISSSSGQVQVKK1025 pKa = 8.89TKK1027 pKa = 10.87GILDD1031 pKa = 3.97RR1032 pKa = 11.84EE1033 pKa = 4.3ATPAYY1038 pKa = 9.14TYY1040 pKa = 11.01INFVVQARR1048 pKa = 11.84DD1049 pKa = 3.34NPTTGTPNSIIQTVRR1064 pKa = 11.84VDD1066 pKa = 3.32VTDD1069 pKa = 4.15FNEE1072 pKa = 4.44LDD1074 pKa = 3.52PTFTGDD1080 pKa = 3.48PFSATLVEE1088 pKa = 5.13DD1089 pKa = 3.87SAAGTLVKK1097 pKa = 10.7QMVVSDD1103 pKa = 4.42PDD1105 pKa = 3.46SSNTIITCQITSGNDD1120 pKa = 2.96LSLFRR1125 pKa = 11.84TNSANSTIIEE1135 pKa = 4.82LNTAIDD1141 pKa = 3.56PDD1143 pKa = 3.76RR1144 pKa = 11.84PKK1146 pKa = 10.92NHH1148 pKa = 7.07PLSYY1152 pKa = 10.88LLIVTCLDD1160 pKa = 3.34NNGAGSYY1167 pKa = 7.87RR1168 pKa = 11.84TATANLSVTITTTNDD1183 pKa = 2.93HH1184 pKa = 6.35TPVVSIPIGSSITLPEE1200 pKa = 3.84TQAIGDD1206 pKa = 3.77VADD1209 pKa = 3.98ISVVDD1214 pKa = 3.63QDD1216 pKa = 3.98YY1217 pKa = 8.57GTDD1220 pKa = 3.48GTVACSIAPTGTFGLTGCTKK1240 pKa = 9.47LTLVSGLDD1248 pKa = 3.58YY1249 pKa = 8.76PTASSYY1255 pKa = 11.69SLTITAVDD1263 pKa = 3.2SGATPRR1269 pKa = 11.84TGSVVVTVTVTDD1281 pKa = 3.6VNRR1284 pKa = 11.84YY1285 pKa = 6.85TPSCSPATYY1294 pKa = 9.74SASVLEE1300 pKa = 4.56TAASFPTALTPATTITCTDD1319 pKa = 3.05GDD1321 pKa = 4.52GNGVYY1326 pKa = 9.89YY1327 pKa = 10.26QLVDD1331 pKa = 3.39SSVPFSVNNLTGVLSLNGPVDD1352 pKa = 3.67YY1353 pKa = 11.04DD1354 pKa = 3.84SAVQTYY1360 pKa = 8.29TILIRR1365 pKa = 11.84AVDD1368 pKa = 3.84PADD1371 pKa = 3.58SSKK1374 pKa = 11.33SGLATATVAVTAANEE1389 pKa = 4.04FTPTFPTATVTLSVYY1404 pKa = 8.32EE1405 pKa = 4.29TVASSTTITTFAATDD1420 pKa = 3.45ADD1422 pKa = 4.25YY1423 pKa = 11.13SPHH1426 pKa = 7.41AITSYY1431 pKa = 11.17AIASGNTGGMFAISNAGVITVASGMTIDD1459 pKa = 4.03YY1460 pKa = 8.33EE1461 pKa = 4.38AVSGSSPYY1469 pKa = 10.56VLQIVATDD1477 pKa = 3.6GGGLTGTGTISIVILDD1493 pKa = 4.4SNDD1496 pKa = 3.41LPPVFSPANYY1506 pKa = 9.14SASVPEE1512 pKa = 4.64GSAAGTTVTTVTATDD1527 pKa = 3.81GDD1529 pKa = 4.04TALNNNNKK1537 pKa = 9.27ILYY1540 pKa = 9.95SKK1542 pKa = 9.93LTGDD1546 pKa = 3.28SAGYY1550 pKa = 9.96FSVNSTNGLILTTGNILNYY1569 pKa = 9.34DD1570 pKa = 3.69TVTQVVFTVTAVDD1583 pKa = 3.82GNGAGPHH1590 pKa = 5.67TATTTVTVAVTDD1602 pKa = 4.2VNLHH1606 pKa = 6.5APSCSPSTVTVNNIRR1621 pKa = 11.84EE1622 pKa = 4.22DD1623 pKa = 3.87VTVGTALTSLTSPVVICTDD1642 pKa = 3.34GDD1644 pKa = 3.84AKK1646 pKa = 10.62DD1647 pKa = 3.48TLTYY1651 pKa = 10.42TMSASASDD1659 pKa = 3.02SHH1661 pKa = 6.92FAVNPTSGAITVSTALDD1678 pKa = 3.55FEE1680 pKa = 4.84TARR1683 pKa = 11.84IHH1685 pKa = 5.09QFEE1688 pKa = 4.54VVVTDD1693 pKa = 3.26SATPAKK1699 pKa = 9.27SVSMAVTINVLDD1711 pKa = 3.9VNEE1714 pKa = 4.81FSPTCTVPTTASMNEE1729 pKa = 3.73AQAVGTAAYY1738 pKa = 9.91SATSCSDD1745 pKa = 3.07SDD1747 pKa = 3.89GTATTLQFALVPGTPFQVSSSGAVTASSALDD1778 pKa = 3.73YY1779 pKa = 11.17DD1780 pKa = 4.16PASATTSYY1788 pKa = 10.77SVRR1791 pKa = 11.84LIVSDD1796 pKa = 3.82NAPTISSLRR1805 pKa = 11.84SSTYY1809 pKa = 6.93TTVVSVTPVNEE1820 pKa = 4.02FAPVLTYY1827 pKa = 9.55TALTASLAEE1836 pKa = 4.35DD1837 pKa = 3.82SGTGTALALKK1847 pKa = 8.8TIGGTSVSMAATDD1860 pKa = 3.67ADD1862 pKa = 3.96RR1863 pKa = 11.84GLNHH1867 pKa = 7.11ATLTWSIVGGNDD1879 pKa = 3.04AGKK1882 pKa = 10.48FSINSASGALQTAAALDD1899 pKa = 3.96RR1900 pKa = 11.84EE1901 pKa = 4.64TAASYY1906 pKa = 9.73TLLVSVTDD1914 pKa = 3.93GGGLSSTANCTVAVSVTDD1932 pKa = 3.68INDD1935 pKa = 3.32NTPVCNPASQTITLYY1950 pKa = 10.56EE1951 pKa = 4.23GEE1953 pKa = 4.19AAKK1956 pKa = 10.64QLVDD1960 pKa = 4.63FGASGRR1966 pKa = 11.84VTDD1969 pKa = 4.87ADD1971 pKa = 3.75SGVNAQLTFSLNNAAALSPDD1991 pKa = 3.46FQLSGTRR1998 pKa = 11.84LSSVRR2003 pKa = 11.84SFSSSDD2009 pKa = 3.33TLSNTLLVVATDD2021 pKa = 4.2GGSSARR2027 pKa = 11.84SATCSVTVNIAVTARR2042 pKa = 11.84PPTFTVANSAVTVSEE2057 pKa = 4.26SSSVGTQVFQFVPNLGTGGTGPLTMSLVDD2086 pKa = 4.94FSPSANSNLFAFDD2099 pKa = 3.58ASTGRR2104 pKa = 11.84VSVNQALDD2112 pKa = 3.65YY2113 pKa = 10.81DD2114 pKa = 4.36SSSTRR2119 pKa = 11.84VPLSS2123 pKa = 3.13

Molecular weight: 218.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A267DPT1|A0A267DPT1_9PLAT Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033127g1 PE=4 SV=1
MM1 pKa = 7.24MKK3 pKa = 10.47AFSNRR8 pKa = 11.84NRR10 pKa = 11.84TNRR13 pKa = 11.84NRR15 pKa = 11.84TNRR18 pKa = 11.84NRR20 pKa = 11.84TNRR23 pKa = 11.84NRR25 pKa = 11.84TNRR28 pKa = 11.84NRR30 pKa = 11.84TNRR33 pKa = 11.84NRR35 pKa = 11.84TNRR38 pKa = 11.84NRR40 pKa = 11.84TNRR43 pKa = 11.84NRR45 pKa = 11.84TNRR48 pKa = 11.84NRR50 pKa = 11.84TNRR53 pKa = 11.84NRR55 pKa = 11.84TNRR58 pKa = 11.84KK59 pKa = 7.81RR60 pKa = 11.84KK61 pKa = 8.56NRR63 pKa = 11.84NRR65 pKa = 11.84INWNRR70 pKa = 11.84TNILIEE76 pKa = 4.28SSLLPP81 pKa = 4.17

Molecular weight: 10.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43466

0

43466

23504603

49

7972

540.8

59.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.943 ± 0.021

2.268 ± 0.009

5.476 ± 0.01

5.805 ± 0.018

3.443 ± 0.009

6.527 ± 0.015

2.209 ± 0.006

3.636 ± 0.009

4.204 ± 0.015

10.01 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.874 ± 0.005

3.383 ± 0.008

5.533 ± 0.015

5.3 ± 0.017

7.071 ± 0.019

8.837 ± 0.014

4.989 ± 0.02

6.005 ± 0.009

1.099 ± 0.004

2.389 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski