Camel associated drosmacovirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Drosmacovirus

Average proteome isoelectric point is 5.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1ENW6|A0A0A1ENW6_9VIRU Putative replicase protein OS=Camel associated drosmacovirus 2 OX=2169877 GN=rep PE=4 SV=1
MM1 pKa = 7.23HH2 pKa = 7.58AEE4 pKa = 4.14DD5 pKa = 4.11TSVPMDD11 pKa = 4.74AGDD14 pKa = 3.47TDD16 pKa = 3.93TAGGIDD22 pKa = 4.26MVTVRR27 pKa = 11.84YY28 pKa = 8.33TEE30 pKa = 4.31TYY32 pKa = 10.54DD33 pKa = 3.25LCTEE37 pKa = 4.02VGKK40 pKa = 10.88LGIIGIHH47 pKa = 5.94TPTLSNLEE55 pKa = 3.92YY56 pKa = 10.44LYY58 pKa = 10.74RR59 pKa = 11.84GLMMNHH65 pKa = 6.98RR66 pKa = 11.84YY67 pKa = 8.63VHH69 pKa = 7.09LDD71 pKa = 3.0GCDD74 pKa = 3.41VVIACASTLPADD86 pKa = 4.05PLQVGTEE93 pKa = 4.04AGDD96 pKa = 3.58VAPQDD101 pKa = 3.55MFNPILYY108 pKa = 9.77KK109 pKa = 10.6AVSNDD114 pKa = 2.93SFNTVINRR122 pKa = 11.84VTVGSSVLDD131 pKa = 3.89DD132 pKa = 5.2EE133 pKa = 4.67ITGGSVRR140 pKa = 11.84KK141 pKa = 10.43VGDD144 pKa = 3.41AFADD148 pKa = 4.29LPQLANAPEE157 pKa = 3.79RR158 pKa = 11.84VYY160 pKa = 11.19YY161 pKa = 10.66GLLADD166 pKa = 4.1PDD168 pKa = 3.85GWKK171 pKa = 10.41KK172 pKa = 11.05AMPQAGLEE180 pKa = 4.02MRR182 pKa = 11.84GLYY185 pKa = 9.9PIVHH189 pKa = 7.19TIYY192 pKa = 9.65STYY195 pKa = 10.36GQQTSPGSNPDD206 pKa = 3.58AQSLPYY212 pKa = 10.61YY213 pKa = 10.42SDD215 pKa = 3.13AGQRR219 pKa = 11.84GSANRR224 pKa = 11.84EE225 pKa = 3.64YY226 pKa = 10.59YY227 pKa = 10.75LRR229 pKa = 11.84GNSVRR234 pKa = 11.84LPRR237 pKa = 11.84LPLCAGTQSSGASGDD252 pKa = 3.54QLPVVTIPTTYY263 pKa = 9.97VACLVMPPAKK273 pKa = 9.91LHH275 pKa = 5.29KK276 pKa = 9.36FYY278 pKa = 11.35YY279 pKa = 9.75RR280 pKa = 11.84MRR282 pKa = 11.84VTWTITFSEE291 pKa = 4.76VVPLTEE297 pKa = 4.1WEE299 pKa = 4.25NPTRR303 pKa = 11.84LWDD306 pKa = 3.36VGTYY310 pKa = 9.38VYY312 pKa = 10.84KK313 pKa = 10.42SDD315 pKa = 4.04YY316 pKa = 10.93DD317 pKa = 3.96EE318 pKa = 3.87QSKK321 pKa = 10.94RR322 pKa = 11.84MDD324 pKa = 3.58TTEE327 pKa = 3.95STVDD331 pKa = 3.34AKK333 pKa = 10.7DD334 pKa = 3.39TTLEE338 pKa = 4.28RR339 pKa = 11.84IMTAGKK345 pKa = 10.16

Molecular weight: 37.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1ENW6|A0A0A1ENW6_9VIRU Putative replicase protein OS=Camel associated drosmacovirus 2 OX=2169877 GN=rep PE=4 SV=1
MM1 pKa = 7.59TNPTWFCGTIWKK13 pKa = 10.06DD14 pKa = 3.07KK15 pKa = 10.28YY16 pKa = 11.03SGSKK20 pKa = 9.26EE21 pKa = 3.76QLAEE25 pKa = 3.88WFRR28 pKa = 11.84LHH30 pKa = 6.38HH31 pKa = 6.4AKK33 pKa = 10.5EE34 pKa = 4.21GVIGEE39 pKa = 4.59EE40 pKa = 3.92ISPTSGKK47 pKa = 9.11IHH49 pKa = 5.16YY50 pKa = 7.91QFRR53 pKa = 11.84IHH55 pKa = 7.37LDD57 pKa = 2.9RR58 pKa = 11.84GEE60 pKa = 4.22TLKK63 pKa = 10.77GWQEE67 pKa = 3.89LIKK70 pKa = 10.75AVGHH74 pKa = 5.86VEE76 pKa = 3.91VCQEE80 pKa = 4.19KK81 pKa = 10.73KK82 pKa = 9.46FTDD85 pKa = 3.82YY86 pKa = 11.12EE87 pKa = 4.37KK88 pKa = 11.18KK89 pKa = 10.35DD90 pKa = 3.77GNWIEE95 pKa = 4.09WPEE98 pKa = 4.07SYY100 pKa = 10.38LGKK103 pKa = 10.26FKK105 pKa = 10.82EE106 pKa = 4.19MPLLFWQEE114 pKa = 3.7QLLEE118 pKa = 4.06QWNEE122 pKa = 3.5QDD124 pKa = 3.66DD125 pKa = 4.12RR126 pKa = 11.84QILFVKK132 pKa = 10.0DD133 pKa = 3.5VKK135 pKa = 11.04GGNGKK140 pKa = 8.19STFGKK145 pKa = 10.05IMEE148 pKa = 4.33ARR150 pKa = 11.84GLEE153 pKa = 4.4EE154 pKa = 4.01VCPVCSDD161 pKa = 4.36DD162 pKa = 4.09YY163 pKa = 11.9NDD165 pKa = 3.55YY166 pKa = 11.02TSFCLEE172 pKa = 4.28YY173 pKa = 9.46PAKK176 pKa = 10.54GYY178 pKa = 10.51IFDD181 pKa = 3.94IPRR184 pKa = 11.84ASSIKK189 pKa = 10.11RR190 pKa = 11.84RR191 pKa = 11.84TALWSGIEE199 pKa = 3.95QIKK202 pKa = 10.96NGLLYY207 pKa = 10.24EE208 pKa = 4.18KK209 pKa = 9.82RR210 pKa = 11.84YY211 pKa = 10.05KK212 pKa = 9.1PRR214 pKa = 11.84KK215 pKa = 8.82VWIEE219 pKa = 3.72PPKK222 pKa = 10.98VIVFTNEE229 pKa = 4.67DD230 pKa = 3.82EE231 pKa = 4.73PPWDD235 pKa = 4.42LLSLDD240 pKa = 3.21RR241 pKa = 11.84WRR243 pKa = 11.84VFDD246 pKa = 3.48VEE248 pKa = 4.73QYY250 pKa = 11.23VNPITYY256 pKa = 10.24NIRR259 pKa = 11.84SHH261 pKa = 6.29WEE263 pKa = 3.2VDD265 pKa = 3.08

Molecular weight: 31.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

739

129

345

246.3

27.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.413 ± 1.279

1.353 ± 0.347

7.307 ± 0.548

6.225 ± 1.709

2.842 ± 0.879

8.525 ± 1.185

1.894 ± 0.159

5.007 ± 0.841

5.277 ± 1.656

7.848 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.665

3.383 ± 0.147

5.142 ± 0.883

3.518 ± 0.275

5.142 ± 0.269

6.089 ± 0.543

7.037 ± 1.57

7.037 ± 0.832

2.706 ± 1.043

5.819 ± 0.606
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski