Microbacterium sp. ABRD_28

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3036 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G6ZJK0|A0A3G6ZJK0_9MICO Rho termination factor OS=Microbacterium sp. ABRD_28 OX=2268461 GN=DT073_03550 PE=4 SV=1
MM1 pKa = 7.59SNTPEE6 pKa = 5.02PDD8 pKa = 4.18DD9 pKa = 4.5SPQGPTYY16 pKa = 10.06EE17 pKa = 4.29GRR19 pKa = 11.84VLPRR23 pKa = 11.84PEE25 pKa = 5.21DD26 pKa = 3.58EE27 pKa = 5.52VVDD30 pKa = 4.0QGAGFDD36 pKa = 3.71VATMVSRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84VLTLVGLGVGAATLAACAATTDD67 pKa = 4.1SPTSATSTATTDD79 pKa = 3.28STATEE84 pKa = 4.3ATDD87 pKa = 3.42STTTTTAAGEE97 pKa = 4.24IPEE100 pKa = 4.53EE101 pKa = 4.35TNGPYY106 pKa = 9.97PADD109 pKa = 3.63GSNGVNVLEE118 pKa = 4.14EE119 pKa = 4.19SGIVRR124 pKa = 11.84SDD126 pKa = 2.76IRR128 pKa = 11.84SSLDD132 pKa = 2.92GGTTAEE138 pKa = 4.3GVPLTFTFSVTDD150 pKa = 3.44IAGGDD155 pKa = 3.69VPFEE159 pKa = 4.1GAAVYY164 pKa = 9.72VWHH167 pKa = 7.64CDD169 pKa = 3.38AQGLYY174 pKa = 10.99SMYY177 pKa = 10.74SEE179 pKa = 4.2GVEE182 pKa = 4.53DD183 pKa = 3.69EE184 pKa = 4.36TFLRR188 pKa = 11.84GIQVVDD194 pKa = 3.58ARR196 pKa = 11.84GEE198 pKa = 4.14ATFQTIVPGCYY209 pKa = 9.22AGRR212 pKa = 11.84WTHH215 pKa = 5.54IHH217 pKa = 6.19FEE219 pKa = 4.36VYY221 pKa = 10.25PDD223 pKa = 3.18IASATDD229 pKa = 3.4VANVIATSQVAFPEE243 pKa = 4.11EE244 pKa = 4.11MLNAVYY250 pKa = 10.42QLDD253 pKa = 4.35AYY255 pKa = 10.82SGSAQNLAQIGGLANDD271 pKa = 4.08NVFSDD276 pKa = 3.84GTEE279 pKa = 3.84LQMGTFSGDD288 pKa = 2.98TSSGYY293 pKa = 10.96VGTLAVGVDD302 pKa = 3.67TDD304 pKa = 3.84TAPGVSAGAGGAGGGQPPSGGGPGNN329 pKa = 3.49

Molecular weight: 33.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G6ZQR2|A0A3G6ZQR2_9MICO Neutral metalloproteinase OS=Microbacterium sp. ABRD_28 OX=2268461 GN=DT073_07260 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3036

0

3036

994539

29

2004

327.6

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.778 ± 0.067

0.481 ± 0.01

6.464 ± 0.038

5.685 ± 0.041

3.156 ± 0.028

9.042 ± 0.042

1.933 ± 0.023

4.5 ± 0.031

1.577 ± 0.029

10.08 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.708 ± 0.016

1.733 ± 0.023

5.61 ± 0.031

2.592 ± 0.022

7.821 ± 0.051

5.324 ± 0.028

5.96 ± 0.033

9.126 ± 0.04

1.538 ± 0.023

1.891 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski