Amino acid dipepetide frequency for Cryobacterium sp. MDB2-A-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.634AlaAla: 20.634 ± 0.21	0.704AlaCys: 0.704 ± 0.027	7.617AlaAsp: 7.617 ± 0.081	7.321AlaGlu: 7.321 ± 0.095	3.984AlaPhe: 3.984 ± 0.063	12.792AlaGly: 12.792 ± 0.136	2.4AlaHis: 2.4 ± 0.053	5.939AlaIle: 5.939 ± 0.085	2.656AlaLys: 2.656 ± 0.056	14.05AlaLeu: 14.05 ± 0.148	2.399AlaMet: 2.399 ± 0.05	2.596AlaAsn: 2.596 ± 0.051	6.245AlaPro: 6.245 ± 0.088	3.553AlaGln: 3.553 ± 0.063	8.617AlaArg: 8.617 ± 0.107	7.741AlaSer: 7.741 ± 0.094	8.149AlaThr: 8.149 ± 0.111	11.171AlaVal: 11.171 ± 0.113	1.868AlaTrp: 1.868 ± 0.039	2.25AlaTyr: 2.25 ± 0.042	0.002AlaXaa: 0.002 ± 0.001
Cys	0.692CysAla: 0.692 ± 0.031	0.059CysCys: 0.059 ± 0.007	0.326CysAsp: 0.326 ± 0.018	0.253CysGlu: 0.253 ± 0.013	0.168CysPhe: 0.168 ± 0.011	0.595CysGly: 0.595 ± 0.024	0.107CysHis: 0.107 ± 0.01	0.225CysIle: 0.225 ± 0.013	0.08CysLys: 0.08 ± 0.008	0.505CysLeu: 0.505 ± 0.022	0.086CysMet: 0.086 ± 0.01	0.124CysAsn: 0.124 ± 0.011	0.34CysPro: 0.34 ± 0.018	0.134CysGln: 0.134 ± 0.011	0.338CysArg: 0.338 ± 0.019	0.364CysSer: 0.364 ± 0.017	0.372CysThr: 0.372 ± 0.022	0.447CysVal: 0.447 ± 0.023	0.092CysTrp: 0.092 ± 0.009	0.122CysTyr: 0.122 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.965AspAla: 7.965 ± 0.084	0.29AspCys: 0.29 ± 0.015	3.327AspAsp: 3.327 ± 0.067	3.377AspGlu: 3.377 ± 0.05	1.899AspPhe: 1.899 ± 0.04	5.177AspGly: 5.177 ± 0.082	1.136AspHis: 1.136 ± 0.033	2.319AspIle: 2.319 ± 0.046	1.121AspLys: 1.121 ± 0.035	6.105AspLeu: 6.105 ± 0.077	0.795AspMet: 0.795 ± 0.027	1.097AspAsn: 1.097 ± 0.034	3.831AspPro: 3.831 ± 0.054	1.621AspGln: 1.621 ± 0.036	4.164AspArg: 4.164 ± 0.076	2.998AspSer: 2.998 ± 0.053	3.214AspThr: 3.214 ± 0.064	4.78AspVal: 4.78 ± 0.068	0.941AspTrp: 0.941 ± 0.028	1.322AspTyr: 1.322 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	6.152GluAla: 6.152 ± 0.088	0.238GluCys: 0.238 ± 0.016	2.329GluAsp: 2.329 ± 0.052	2.383GluGlu: 2.383 ± 0.057	1.728GluPhe: 1.728 ± 0.035	3.209GluGly: 3.209 ± 0.057	1.33GluHis: 1.33 ± 0.035	2.584GluIle: 2.584 ± 0.053	1.323GluLys: 1.323 ± 0.038	6.254GluLeu: 6.254 ± 0.077	0.835GluMet: 0.835 ± 0.03	1.242GluAsn: 1.242 ± 0.037	2.778GluPro: 2.778 ± 0.05	1.831GluGln: 1.831 ± 0.043	4.242GluArg: 4.242 ± 0.07	2.902GluSer: 2.902 ± 0.048	3.115GluThr: 3.115 ± 0.055	3.858GluVal: 3.858 ± 0.058	0.738GluTrp: 0.738 ± 0.026	1.05GluTyr: 1.05 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.256PheAla: 4.256 ± 0.062	0.194PheCys: 0.194 ± 0.014	2.265PheAsp: 2.265 ± 0.044	1.695PheGlu: 1.695 ± 0.039	1.19PhePhe: 1.19 ± 0.036	3.614PheGly: 3.614 ± 0.052	0.602PheHis: 0.602 ± 0.023	1.337PheIle: 1.337 ± 0.04	0.56PheLys: 0.56 ± 0.023	3.19PheLeu: 3.19 ± 0.056	0.509PheMet: 0.509 ± 0.021	0.777PheAsn: 0.777 ± 0.027	1.542PhePro: 1.542 ± 0.039	0.86PheGln: 0.86 ± 0.024	1.794PheArg: 1.794 ± 0.037	2.002PheSer: 2.002 ± 0.045	2.288PheThr: 2.288 ± 0.05	2.865PheVal: 2.865 ± 0.048	0.475PheTrp: 0.475 ± 0.02	0.729PheTyr: 0.729 ± 0.028	0.001PheXaa: 0.001 ± 0.001
Gly	10.501GlyAla: 10.501 ± 0.101	0.622GlyCys: 0.622 ± 0.024	4.504GlyAsp: 4.504 ± 0.072	4.124GlyGlu: 4.124 ± 0.066	3.387GlyPhe: 3.387 ± 0.054	7.359GlyGly: 7.359 ± 0.109	1.917GlyHis: 1.917 ± 0.048	5.128GlyIle: 5.128 ± 0.073	2.243GlyLys: 2.243 ± 0.047	9.713GlyLeu: 9.713 ± 0.105	1.791GlyMet: 1.791 ± 0.039	2.036GlyAsn: 2.036 ± 0.046	4.029GlyPro: 4.029 ± 0.056	2.578GlyGln: 2.578 ± 0.046	6.087GlyArg: 6.087 ± 0.083	6.023GlySer: 6.023 ± 0.082	6.455GlyThr: 6.455 ± 0.091	7.677GlyVal: 7.677 ± 0.08	1.566GlyTrp: 1.566 ± 0.042	2.264GlyTyr: 2.264 ± 0.043	0.001GlyXaa: 0.001 ± 0.001
His	2.303HisAla: 2.303 ± 0.045	0.121HisCys: 0.121 ± 0.011	1.224HisAsp: 1.224 ± 0.035	1.045HisGlu: 1.045 ± 0.037	0.569HisPhe: 0.569 ± 0.02	1.985HisGly: 1.985 ± 0.044	0.507HisHis: 0.507 ± 0.025	0.785HisIle: 0.785 ± 0.025	0.322HisLys: 0.322 ± 0.015	2.061HisLeu: 2.061 ± 0.052	0.277HisMet: 0.277 ± 0.016	0.447HisAsn: 0.447 ± 0.022	1.526HisPro: 1.526 ± 0.04	0.552HisGln: 0.552 ± 0.023	1.476HisArg: 1.476 ± 0.037	1.116HisSer: 1.116 ± 0.029	1.098HisThr: 1.098 ± 0.034	1.45HisVal: 1.45 ± 0.037	0.283HisTrp: 0.283 ± 0.015	0.426HisTyr: 0.426 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.479IleAla: 6.479 ± 0.092	0.282IleCys: 0.282 ± 0.015	3.443IleAsp: 3.443 ± 0.063	2.766IleGlu: 2.766 ± 0.051	1.319IlePhe: 1.319 ± 0.033	4.935IleGly: 4.935 ± 0.073	0.809IleHis: 0.809 ± 0.026	2.073IleIle: 2.073 ± 0.048	0.909IleLys: 0.909 ± 0.033	4.214IleLeu: 4.214 ± 0.07	0.705IleMet: 0.705 ± 0.027	1.127IleAsn: 1.127 ± 0.038	2.561IlePro: 2.561 ± 0.056	1.044IleGln: 1.044 ± 0.031	2.953IleArg: 2.953 ± 0.047	2.626IleSer: 2.626 ± 0.052	3.128IleThr: 3.128 ± 0.054	4.905IleVal: 4.905 ± 0.075	0.495IleTrp: 0.495 ± 0.022	0.826IleTyr: 0.826 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	2.616LysAla: 2.616 ± 0.063	0.081LysCys: 0.081 ± 0.01	1.158LysAsp: 1.158 ± 0.034	0.927LysGlu: 0.927 ± 0.032	0.594LysPhe: 0.594 ± 0.023	1.555LysGly: 1.555 ± 0.044	0.434LysHis: 0.434 ± 0.02	1.075LysIle: 1.075 ± 0.03	0.825LysLys: 0.825 ± 0.033	2.081LysLeu: 2.081 ± 0.042	0.437LysMet: 0.437 ± 0.018	0.657LysAsn: 0.657 ± 0.024	1.275LysPro: 1.275 ± 0.037	0.717LysGln: 0.717 ± 0.025	1.597LysArg: 1.597 ± 0.036	1.394LysSer: 1.394 ± 0.046	1.459LysThr: 1.459 ± 0.04	1.731LysVal: 1.731 ± 0.042	0.291LysTrp: 0.291 ± 0.014	0.496LysTyr: 0.496 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	15.313LeuAla: 15.313 ± 0.155	0.58LeuCys: 0.58 ± 0.021	6.524LeuAsp: 6.524 ± 0.086	4.959LeuGlu: 4.959 ± 0.082	3.185LeuPhe: 3.185 ± 0.06	9.857LeuGly: 9.857 ± 0.104	1.891LeuHis: 1.891 ± 0.047	4.755LeuIle: 4.755 ± 0.078	1.968LeuLys: 1.968 ± 0.045	11.029LeuLeu: 11.029 ± 0.115	1.696LeuMet: 1.696 ± 0.038	2.208LeuAsn: 2.208 ± 0.046	5.685LeuPro: 5.685 ± 0.074	2.409LeuGln: 2.409 ± 0.049	7.011LeuArg: 7.011 ± 0.093	6.371LeuSer: 6.371 ± 0.069	6.959LeuThr: 6.959 ± 0.087	9.751LeuVal: 9.751 ± 0.104	1.224LeuTrp: 1.224 ± 0.034	1.737LeuTyr: 1.737 ± 0.045	0.002LeuXaa: 0.002 ± 0.001
Met	2.022MetAla: 2.022 ± 0.046	0.103MetCys: 0.103 ± 0.01	0.761MetAsp: 0.761 ± 0.027	0.6MetGlu: 0.6 ± 0.024	0.54MetPhe: 0.54 ± 0.023	1.224MetGly: 1.224 ± 0.036	0.316MetHis: 0.316 ± 0.015	0.925MetIle: 0.925 ± 0.032	0.5MetLys: 0.5 ± 0.021	1.836MetLeu: 1.836 ± 0.042	0.349MetMet: 0.349 ± 0.02	0.527MetAsn: 0.527 ± 0.02	1.073MetPro: 1.073 ± 0.032	0.54MetGln: 0.54 ± 0.022	1.245MetArg: 1.245 ± 0.03	1.453MetSer: 1.453 ± 0.031	1.655MetThr: 1.655 ± 0.036	1.365MetVal: 1.365 ± 0.041	0.211MetTrp: 0.211 ± 0.015	0.272MetTyr: 0.272 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.723AsnAla: 2.723 ± 0.054	0.13AsnCys: 0.13 ± 0.01	1.267AsnAsp: 1.267 ± 0.032	1.054AsnGlu: 1.054 ± 0.03	0.714AsnPhe: 0.714 ± 0.025	2.187AsnGly: 2.187 ± 0.054	0.416AsnHis: 0.416 ± 0.02	0.972AsnIle: 0.972 ± 0.03	0.527AsnLys: 0.527 ± 0.024	2.268AsnLeu: 2.268 ± 0.05	0.368AsnMet: 0.368 ± 0.019	0.599AsnAsn: 0.599 ± 0.031	1.773AsnPro: 1.773 ± 0.045	0.702AsnGln: 0.702 ± 0.033	1.537AsnArg: 1.537 ± 0.041	1.223AsnSer: 1.223 ± 0.036	1.426AsnThr: 1.426 ± 0.039	1.803AsnVal: 1.803 ± 0.044	0.348AsnTrp: 0.348 ± 0.018	0.573AsnTyr: 0.573 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.622ProAla: 7.622 ± 0.109	0.205ProCys: 0.205 ± 0.013	3.625ProAsp: 3.625 ± 0.054	3.424ProGlu: 3.424 ± 0.054	1.736ProPhe: 1.736 ± 0.042	5.488ProGly: 5.488 ± 0.082	1.05ProHis: 1.05 ± 0.037	2.299ProIle: 2.299 ± 0.046	1.105ProLys: 1.105 ± 0.034	4.963ProLeu: 4.963 ± 0.066	0.943ProMet: 0.943 ± 0.031	1.216ProAsn: 1.216 ± 0.034	2.272ProPro: 2.272 ± 0.052	1.367ProGln: 1.367 ± 0.036	3.256ProArg: 3.256 ± 0.063	3.186ProSer: 3.186 ± 0.056	3.641ProThr: 3.641 ± 0.07	5.162ProVal: 5.162 ± 0.076	0.773ProTrp: 0.773 ± 0.026	1.011ProTyr: 1.011 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.658GlnAla: 3.658 ± 0.062	0.115GlnCys: 0.115 ± 0.01	1.299GlnAsp: 1.299 ± 0.033	1.073GlnGlu: 1.073 ± 0.032	0.9GlnPhe: 0.9 ± 0.027	2.108GlnGly: 2.108 ± 0.041	0.561GlnHis: 0.561 ± 0.025	1.411GlnIle: 1.411 ± 0.037	0.741GlnLys: 0.741 ± 0.026	3.142GlnLeu: 3.142 ± 0.057	0.501GlnMet: 0.501 ± 0.018	0.683GlnAsn: 0.683 ± 0.026	1.521GlnPro: 1.521 ± 0.037	1.078GlnGln: 1.078 ± 0.033	2.027GlnArg: 2.027 ± 0.043	1.591GlnSer: 1.591 ± 0.036	1.569GlnThr: 1.569 ± 0.037	2.403GlnVal: 2.403 ± 0.042	0.415GlnTrp: 0.415 ± 0.02	0.609GlnTyr: 0.609 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	8.163ArgAla: 8.163 ± 0.109	0.322ArgCys: 0.322 ± 0.018	3.819ArgAsp: 3.819 ± 0.061	3.626ArgGlu: 3.626 ± 0.065	2.428ArgPhe: 2.428 ± 0.051	5.003ArgGly: 5.003 ± 0.077	1.511ArgHis: 1.511 ± 0.037	3.52ArgIle: 3.52 ± 0.056	1.476ArgLys: 1.476 ± 0.035	7.339ArgLeu: 7.339 ± 0.106	1.596ArgMet: 1.596 ± 0.033	1.493ArgAsn: 1.493 ± 0.036	3.638ArgPro: 3.638 ± 0.06	1.957ArgGln: 1.957 ± 0.039	5.761ArgArg: 5.761 ± 0.107	4.153ArgSer: 4.153 ± 0.059	4.17ArgThr: 4.17 ± 0.062	5.485ArgVal: 5.485 ± 0.076	0.979ArgTrp: 0.979 ± 0.028	1.549ArgTyr: 1.549 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	7.81SerAla: 7.81 ± 0.083	0.329SerCys: 0.329 ± 0.017	3.055SerAsp: 3.055 ± 0.052	2.688SerGlu: 2.688 ± 0.044	2.121SerPhe: 2.121 ± 0.045	6.335SerGly: 6.335 ± 0.079	1.06SerHis: 1.06 ± 0.027	2.977SerIle: 2.977 ± 0.055	1.179SerLys: 1.179 ± 0.038	5.937SerLeu: 5.937 ± 0.074	1.221SerMet: 1.221 ± 0.032	1.341SerAsn: 1.341 ± 0.039	3.335SerPro: 3.335 ± 0.06	1.539SerGln: 1.539 ± 0.036	3.912SerArg: 3.912 ± 0.061	3.78SerSer: 3.78 ± 0.066	4.181SerThr: 4.181 ± 0.063	5.335SerVal: 5.335 ± 0.071	0.95SerTrp: 0.95 ± 0.028	1.346SerTyr: 1.346 ± 0.035	0.001SerXaa: 0.001 ± 0.001
Thr	8.661ThrAla: 8.661 ± 0.109	0.311ThrCys: 0.311 ± 0.017	3.878ThrAsp: 3.878 ± 0.065	3.233ThrGlu: 3.233 ± 0.058	1.929ThrPhe: 1.929 ± 0.048	6.753ThrGly: 6.753 ± 0.093	1.145ThrHis: 1.145 ± 0.032	3.288ThrIle: 3.288 ± 0.053	1.402ThrLys: 1.402 ± 0.043	6.524ThrLeu: 6.524 ± 0.082	0.982ThrMet: 0.982 ± 0.03	1.425ThrAsn: 1.425 ± 0.041	4.223ThrPro: 4.223 ± 0.071	1.508ThrGln: 1.508 ± 0.037	3.845ThrArg: 3.845 ± 0.067	3.807ThrSer: 3.807 ± 0.065	4.377ThrThr: 4.377 ± 0.087	6.449ThrVal: 6.449 ± 0.084	0.875ThrTrp: 0.875 ± 0.027	1.153ThrTyr: 1.153 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	11.254ValAla: 11.254 ± 0.117	0.508ValCys: 0.508 ± 0.021	5.172ValAsp: 5.172 ± 0.078	4.055ValGlu: 4.055 ± 0.063	3.017ValPhe: 3.017 ± 0.052	6.942ValGly: 6.942 ± 0.088	1.739ValHis: 1.739 ± 0.038	4.579ValIle: 4.579 ± 0.076	1.746ValLys: 1.746 ± 0.047	9.69ValLeu: 9.69 ± 0.113	1.439ValMet: 1.439 ± 0.04	2.039ValAsn: 2.039 ± 0.043	4.867ValPro: 4.867 ± 0.073	2.234ValGln: 2.234 ± 0.049	5.424ValArg: 5.424 ± 0.07	5.471ValSer: 5.471 ± 0.071	6.449ValThr: 6.449 ± 0.097	8.196ValVal: 8.196 ± 0.084	1.044ValTrp: 1.044 ± 0.037	1.611ValTyr: 1.611 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.525TrpAla: 1.525 ± 0.035	0.097TrpCys: 0.097 ± 0.009	0.71TrpAsp: 0.71 ± 0.026	0.525TrpGlu: 0.525 ± 0.021	0.545TrpPhe: 0.545 ± 0.024	0.989TrpGly: 0.989 ± 0.032	0.305TrpHis: 0.305 ± 0.014	0.691TrpIle: 0.691 ± 0.023	0.317TrpLys: 0.317 ± 0.016	1.871TrpLeu: 1.871 ± 0.044	0.306TrpMet: 0.306 ± 0.016	0.448TrpAsn: 0.448 ± 0.018	0.704TrpPro: 0.704 ± 0.026	0.577TrpGln: 0.577 ± 0.023	1.073TrpArg: 1.073 ± 0.033	0.928TrpSer: 0.928 ± 0.029	0.899TrpThr: 0.899 ± 0.026	1.075TrpVal: 1.075 ± 0.029	0.302TrpTrp: 0.302 ± 0.017	0.294TrpTyr: 0.294 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.338TyrAla: 2.338 ± 0.046	0.137TyrCys: 0.137 ± 0.012	1.175TyrAsp: 1.175 ± 0.032	0.994TyrGlu: 0.994 ± 0.032	0.792TyrPhe: 0.792 ± 0.028	1.898TyrGly: 1.898 ± 0.041	0.336TyrHis: 0.336 ± 0.02	0.716TyrIle: 0.716 ± 0.026	0.402TyrLys: 0.402 ± 0.019	2.342TyrLeu: 2.342 ± 0.05	0.278TyrMet: 0.278 ± 0.016	0.525TyrAsn: 0.525 ± 0.024	1.128TyrPro: 1.128 ± 0.03	0.606TyrGln: 0.606 ± 0.023	1.627TyrArg: 1.627 ± 0.042	1.303TyrSer: 1.303 ± 0.03	1.163TyrThr: 1.163 ± 0.03	1.568TyrVal: 1.568 ± 0.038	0.311TyrTrp: 0.311 ± 0.016	0.491TyrTyr: 0.491 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3588 proteins (1178553 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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