Ohlsdorf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ohlsrhavirus; Ohlsdorf ohlsrhavirus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291I346|A0A291I346_9RHAB Nucleocapsid protein OS=Ohlsdorf virus OX=2040592 GN=N PE=4 SV=1
MM1 pKa = 7.69CIVDD5 pKa = 3.67QGCHH9 pKa = 4.76HH10 pKa = 7.15SIYY13 pKa = 10.55RR14 pKa = 11.84FLSTNLISVNMVVKK28 pKa = 10.52RR29 pKa = 11.84VSTDD33 pKa = 2.83PTKK36 pKa = 10.62RR37 pKa = 11.84VTVTHH42 pKa = 5.95MNIATDD48 pKa = 3.76RR49 pKa = 11.84PIAYY53 pKa = 9.14PSEE56 pKa = 4.15WFDD59 pKa = 4.18NPLNDD64 pKa = 4.47KK65 pKa = 10.69PKK67 pKa = 10.51ILIAAEE73 pKa = 3.99GDD75 pKa = 3.08ITRR78 pKa = 11.84AQLHH82 pKa = 5.85ATVQTNFPEE91 pKa = 4.81GDD93 pKa = 3.46LDD95 pKa = 3.64HH96 pKa = 6.95RR97 pKa = 11.84VAMMFLRR104 pKa = 11.84HH105 pKa = 4.56EE106 pKa = 4.49MMRR109 pKa = 11.84KK110 pKa = 8.48SHH112 pKa = 6.29MLGEE116 pKa = 4.7DD117 pKa = 2.78WTSFGRR123 pKa = 11.84AIGVRR128 pKa = 11.84GGDD131 pKa = 3.11ITIGNLVIFDD141 pKa = 3.82QTEE144 pKa = 3.9TRR146 pKa = 11.84PDD148 pKa = 3.59VIDD151 pKa = 5.64GEE153 pKa = 4.93DD154 pKa = 3.64CMTDD158 pKa = 3.51SEE160 pKa = 4.55VLKK163 pKa = 10.48YY164 pKa = 10.51IILICSMYY172 pKa = 10.67RR173 pKa = 11.84LAQISRR179 pKa = 11.84DD180 pKa = 3.63DD181 pKa = 3.5YY182 pKa = 10.44RR183 pKa = 11.84TNVVNNVTALIEE195 pKa = 4.41GLDD198 pKa = 3.81GEE200 pKa = 4.57AVEE203 pKa = 6.12FSTQILSYY211 pKa = 9.98RR212 pKa = 11.84KK213 pKa = 7.61WLAYY217 pKa = 9.86HH218 pKa = 7.12PYY220 pKa = 9.09IKK222 pKa = 10.23MMAAIDD228 pKa = 3.72MYY230 pKa = 11.28LNEE233 pKa = 4.66FPYY236 pKa = 10.19HH237 pKa = 5.93QYY239 pKa = 10.96ASARR243 pKa = 11.84IGTIVTRR250 pKa = 11.84FKK252 pKa = 11.02DD253 pKa = 3.62CSVLVATKK261 pKa = 9.93TITEE265 pKa = 4.07ALGLTFDD272 pKa = 5.25TFAEE276 pKa = 4.79WIWTARR282 pKa = 11.84CANQFVNVIKK292 pKa = 10.73GGEE295 pKa = 4.0EE296 pKa = 3.59MDD298 pKa = 3.66NQRR301 pKa = 11.84SYY303 pKa = 12.2SMYY306 pKa = 10.64FMDD309 pKa = 6.09FGLSPKK315 pKa = 10.01SPYY318 pKa = 10.13SATVNADD325 pKa = 2.96LHH327 pKa = 6.59LFYY330 pKa = 9.89HH331 pKa = 6.8TIGVSSGLEE340 pKa = 3.48RR341 pKa = 11.84SRR343 pKa = 11.84NARR346 pKa = 11.84YY347 pKa = 9.63IGNPEE352 pKa = 3.92HH353 pKa = 7.12NNIINNAMVIHH364 pKa = 5.84YY365 pKa = 10.03VLGTFSTFDD374 pKa = 3.33AQFSADD380 pKa = 3.5GVPIEE385 pKa = 5.38LEE387 pKa = 4.15ADD389 pKa = 3.72DD390 pKa = 6.15LVDD393 pKa = 6.19DD394 pKa = 5.57DD395 pKa = 6.02LPGDD399 pKa = 3.87NSPEE403 pKa = 3.65LWLGYY408 pKa = 9.94IISKK412 pKa = 9.99NGRR415 pKa = 11.84IPPIVARR422 pKa = 11.84QSILEE427 pKa = 4.09WNQFPGCRR435 pKa = 11.84EE436 pKa = 3.93SSIGLYY442 pKa = 9.92LYY444 pKa = 10.73KK445 pKa = 10.4RR446 pKa = 11.84AQTLMVSLVAPAQQ459 pKa = 3.42

Molecular weight: 51.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291I327|A0A291I327_9RHAB GDP polyribonucleotidyltransferase OS=Ohlsdorf virus OX=2040592 GN=L PE=4 SV=1
MM1 pKa = 7.49SSNSALLVAYY11 pKa = 9.05NLRR14 pKa = 11.84VNASLDD20 pKa = 3.35VKK22 pKa = 10.54FRR24 pKa = 11.84HH25 pKa = 6.26PPKK28 pKa = 10.28AYY30 pKa = 9.33KK31 pKa = 10.48DD32 pKa = 3.49LMRR35 pKa = 11.84ALEE38 pKa = 4.7LIKK41 pKa = 10.76DD42 pKa = 3.69NYY44 pKa = 11.03KK45 pKa = 10.87GFTRR49 pKa = 11.84DD50 pKa = 2.91KK51 pKa = 9.74EE52 pKa = 4.54LIFFFYY58 pKa = 10.94VMSGFFSKK66 pKa = 10.9YY67 pKa = 8.59INKK70 pKa = 9.71IGNFYY75 pKa = 10.69NYY77 pKa = 9.75QGTLSGVFNLMCKK90 pKa = 10.37NITPRR95 pKa = 11.84FPINYY100 pKa = 7.68EE101 pKa = 3.91FNGDD105 pKa = 3.97DD106 pKa = 5.08GGIHH110 pKa = 6.92PNFLITFHH118 pKa = 6.92LSVRR122 pKa = 11.84EE123 pKa = 3.75SDD125 pKa = 3.26MSYY128 pKa = 11.0QSLYY132 pKa = 10.98DD133 pKa = 3.48YY134 pKa = 10.13MKK136 pKa = 9.25EE137 pKa = 3.96HH138 pKa = 6.79SPSKK142 pKa = 8.92YY143 pKa = 9.47RR144 pKa = 11.84KK145 pKa = 7.97RR146 pKa = 11.84NKK148 pKa = 9.74VKK150 pKa = 10.16MSIFDD155 pKa = 4.61DD156 pKa = 4.33YY157 pKa = 11.18PVSITVKK164 pKa = 9.63QKK166 pKa = 10.5VFILTSIKK174 pKa = 10.67SSATKK179 pKa = 10.6NSFSPEE185 pKa = 3.65LKK187 pKa = 10.61

Molecular weight: 21.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3708

187

2160

741.6

84.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.585 ± 0.859

1.375 ± 0.257

5.556 ± 0.393

5.367 ± 0.424

4.396 ± 0.493

4.962 ± 0.375

2.778 ± 0.23

7.551 ± 0.621

6.823 ± 0.758

9.628 ± 1.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.388

5.663 ± 0.544

4.746 ± 0.453

3.182 ± 0.371

4.773 ± 0.318

8.522 ± 0.445

6.095 ± 0.553

5.663 ± 0.444

1.591 ± 0.332

4.045 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski