Amino acid dipepetide frequency for Mycobacterium phage Pipefish

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.786AlaAla: 15.786 ± 1.271
1.049AlaCys: 1.049 ± 0.239
7.574AlaAsp: 7.574 ± 0.606
8.441AlaGlu: 8.441 ± 0.65
2.464AlaPhe: 2.464 ± 0.347
9.49AlaGly: 9.49 ± 1.1
1.688AlaHis: 1.688 ± 0.319
5.521AlaIle: 5.521 ± 0.426
3.239AlaLys: 3.239 ± 0.353
9.855AlaLeu: 9.855 ± 0.92
2.601AlaMet: 2.601 ± 0.356
3.513AlaAsn: 3.513 ± 0.466
5.749AlaPro: 5.749 ± 0.638
2.737AlaGln: 2.737 ± 0.421
7.847AlaArg: 7.847 ± 0.681
5.384AlaSer: 5.384 ± 0.505
6.387AlaThr: 6.387 ± 0.574
9.216AlaVal: 9.216 ± 0.566
2.509AlaTrp: 2.509 ± 0.327
2.418AlaTyr: 2.418 ± 0.357
0.0AlaXaa: 0.0 ± 0.0
Cys
1.049CysAla: 1.049 ± 0.222
0.091CysCys: 0.091 ± 0.058
0.867CysAsp: 0.867 ± 0.215
0.593CysGlu: 0.593 ± 0.181
0.274CysPhe: 0.274 ± 0.135
1.414CysGly: 1.414 ± 0.358
0.502CysHis: 0.502 ± 0.171
0.319CysIle: 0.319 ± 0.126
0.228CysLys: 0.228 ± 0.095
0.593CysLeu: 0.593 ± 0.181
0.228CysMet: 0.228 ± 0.102
0.411CysAsn: 0.411 ± 0.141
1.232CysPro: 1.232 ± 0.268
0.319CysGln: 0.319 ± 0.124
0.958CysArg: 0.958 ± 0.235
0.73CysSer: 0.73 ± 0.201
0.411CysThr: 0.411 ± 0.159
0.639CysVal: 0.639 ± 0.175
0.365CysTrp: 0.365 ± 0.144
0.137CysTyr: 0.137 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
7.619AspAla: 7.619 ± 0.407
0.547AspCys: 0.547 ± 0.157
5.794AspAsp: 5.794 ± 0.724
5.201AspGlu: 5.201 ± 0.536
1.962AspPhe: 1.962 ± 0.294
6.296AspGly: 6.296 ± 0.533
1.779AspHis: 1.779 ± 0.307
2.829AspIle: 2.829 ± 0.312
1.779AspLys: 1.779 ± 0.214
5.612AspLeu: 5.612 ± 0.437
1.277AspMet: 1.277 ± 0.205
2.053AspAsn: 2.053 ± 0.253
5.019AspPro: 5.019 ± 0.522
2.646AspGln: 2.646 ± 0.382
4.836AspArg: 4.836 ± 0.578
3.559AspSer: 3.559 ± 0.455
3.65AspThr: 3.65 ± 0.321
4.243AspVal: 4.243 ± 0.485
1.004AspTrp: 1.004 ± 0.206
0.958AspTyr: 0.958 ± 0.183
0.0AspXaa: 0.0 ± 0.0
Glu
6.798GluAla: 6.798 ± 0.562
1.049GluCys: 1.049 ± 0.246
4.243GluAsp: 4.243 ± 0.582
2.327GluGlu: 2.327 ± 0.424
1.962GluPhe: 1.962 ± 0.259
4.334GluGly: 4.334 ± 0.446
1.232GluHis: 1.232 ± 0.213
3.832GluIle: 3.832 ± 0.402
2.281GluLys: 2.281 ± 0.298
4.197GluLeu: 4.197 ± 0.468
1.734GluMet: 1.734 ± 0.288
1.46GluAsn: 1.46 ± 0.247
3.741GluPro: 3.741 ± 0.435
2.053GluGln: 2.053 ± 0.295
4.699GluArg: 4.699 ± 0.473
2.874GluSer: 2.874 ± 0.376
3.376GluThr: 3.376 ± 0.45
4.334GluVal: 4.334 ± 0.494
1.277GluTrp: 1.277 ± 0.238
1.414GluTyr: 1.414 ± 0.279
0.0GluXaa: 0.0 ± 0.0
Phe
2.966PheAla: 2.966 ± 0.317
0.182PheCys: 0.182 ± 0.085
2.692PheAsp: 2.692 ± 0.371
1.551PheGlu: 1.551 ± 0.316
0.456PhePhe: 0.456 ± 0.125
2.874PheGly: 2.874 ± 0.438
0.411PheHis: 0.411 ± 0.15
0.821PheIle: 0.821 ± 0.184
0.684PheLys: 0.684 ± 0.154
2.053PheLeu: 2.053 ± 0.227
0.456PheMet: 0.456 ± 0.132
0.684PheAsn: 0.684 ± 0.128
1.597PhePro: 1.597 ± 0.279
0.776PheGln: 0.776 ± 0.182
1.779PheArg: 1.779 ± 0.319
1.141PheSer: 1.141 ± 0.235
1.916PheThr: 1.916 ± 0.287
2.327PheVal: 2.327 ± 0.353
0.639PheTrp: 0.639 ± 0.171
0.547PheTyr: 0.547 ± 0.15
0.0PheXaa: 0.0 ± 0.0
Gly
7.893GlyAla: 7.893 ± 1.324
1.095GlyCys: 1.095 ± 0.242
5.11GlyAsp: 5.11 ± 0.452
5.11GlyGlu: 5.11 ± 0.482
2.555GlyPhe: 2.555 ± 0.34
12.912GlyGly: 12.912 ± 2.695
1.551GlyHis: 1.551 ± 0.261
4.973GlyIle: 4.973 ± 0.594
4.152GlyLys: 4.152 ± 0.464
7.893GlyLeu: 7.893 ± 0.712
2.372GlyMet: 2.372 ± 0.395
3.239GlyAsn: 3.239 ± 0.416
4.015GlyPro: 4.015 ± 0.509
2.601GlyGln: 2.601 ± 0.457
5.475GlyArg: 5.475 ± 0.612
5.84GlySer: 5.84 ± 0.727
6.387GlyThr: 6.387 ± 0.684
7.254GlyVal: 7.254 ± 0.668
1.916GlyTrp: 1.916 ± 0.306
2.007GlyTyr: 2.007 ± 0.255
0.0GlyXaa: 0.0 ± 0.0
His
2.099HisAla: 2.099 ± 0.32
0.274HisCys: 0.274 ± 0.113
1.186HisAsp: 1.186 ± 0.25
0.776HisGlu: 0.776 ± 0.241
0.639HisPhe: 0.639 ± 0.16
1.506HisGly: 1.506 ± 0.265
0.456HisHis: 0.456 ± 0.152
0.593HisIle: 0.593 ± 0.196
0.456HisLys: 0.456 ± 0.157
1.277HisLeu: 1.277 ± 0.251
0.502HisMet: 0.502 ± 0.133
0.502HisAsn: 0.502 ± 0.151
1.095HisPro: 1.095 ± 0.244
0.456HisGln: 0.456 ± 0.173
1.779HisArg: 1.779 ± 0.298
0.912HisSer: 0.912 ± 0.218
1.232HisThr: 1.232 ± 0.241
1.186HisVal: 1.186 ± 0.252
0.456HisTrp: 0.456 ± 0.136
0.639HisTyr: 0.639 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
5.703IleAla: 5.703 ± 0.476
0.319IleCys: 0.319 ± 0.126
4.289IleAsp: 4.289 ± 0.413
3.604IleGlu: 3.604 ± 0.355
1.004IlePhe: 1.004 ± 0.18
3.696IleGly: 3.696 ± 0.494
0.821IleHis: 0.821 ± 0.155
1.825IleIle: 1.825 ± 0.289
1.506IleLys: 1.506 ± 0.324
3.285IleLeu: 3.285 ± 0.402
0.684IleMet: 0.684 ± 0.177
1.551IleAsn: 1.551 ± 0.307
2.829IlePro: 2.829 ± 0.313
1.779IleGln: 1.779 ± 0.295
3.513IleArg: 3.513 ± 0.416
2.783IleSer: 2.783 ± 0.323
3.239IleThr: 3.239 ± 0.458
4.243IleVal: 4.243 ± 0.421
0.411IleTrp: 0.411 ± 0.146
0.776IleTyr: 0.776 ± 0.173
0.0IleXaa: 0.0 ± 0.0
Lys
3.741LysAla: 3.741 ± 0.518
0.319LysCys: 0.319 ± 0.123
1.779LysAsp: 1.779 ± 0.33
0.958LysGlu: 0.958 ± 0.197
1.141LysPhe: 1.141 ± 0.18
2.874LysGly: 2.874 ± 0.296
0.411LysHis: 0.411 ± 0.148
1.962LysIle: 1.962 ± 0.348
0.912LysLys: 0.912 ± 0.206
2.966LysLeu: 2.966 ± 0.319
0.639LysMet: 0.639 ± 0.186
1.186LysAsn: 1.186 ± 0.215
1.779LysPro: 1.779 ± 0.266
1.049LysGln: 1.049 ± 0.263
2.281LysArg: 2.281 ± 0.433
2.007LysSer: 2.007 ± 0.342
1.369LysThr: 1.369 ± 0.258
3.194LysVal: 3.194 ± 0.362
0.684LysTrp: 0.684 ± 0.144
0.912LysTyr: 0.912 ± 0.212
0.0LysXaa: 0.0 ± 0.0
Leu
8.942LeuAla: 8.942 ± 0.861
0.867LeuCys: 0.867 ± 0.228
5.338LeuAsp: 5.338 ± 0.527
3.376LeuGlu: 3.376 ± 0.496
2.007LeuPhe: 2.007 ± 0.339
7.619LeuGly: 7.619 ± 0.807
1.551LeuHis: 1.551 ± 0.293
3.924LeuIle: 3.924 ± 0.478
2.464LeuLys: 2.464 ± 0.397
5.612LeuLeu: 5.612 ± 0.628
1.506LeuMet: 1.506 ± 0.224
2.099LeuAsn: 2.099 ± 0.273
4.745LeuPro: 4.745 ± 0.873
1.916LeuGln: 1.916 ± 0.307
5.886LeuArg: 5.886 ± 0.658
4.562LeuSer: 4.562 ± 0.572
6.433LeuThr: 6.433 ± 0.703
5.703LeuVal: 5.703 ± 0.517
1.323LeuTrp: 1.323 ± 0.223
2.099LeuTyr: 2.099 ± 0.342
0.0LeuXaa: 0.0 ± 0.0
Met
3.102MetAla: 3.102 ± 0.432
0.182MetCys: 0.182 ± 0.089
0.821MetAsp: 0.821 ± 0.189
1.049MetGlu: 1.049 ± 0.201
0.456MetPhe: 0.456 ± 0.136
1.414MetGly: 1.414 ± 0.249
0.274MetHis: 0.274 ± 0.105
1.232MetIle: 1.232 ± 0.21
0.73MetLys: 0.73 ± 0.166
1.506MetLeu: 1.506 ± 0.279
0.456MetMet: 0.456 ± 0.128
0.502MetAsn: 0.502 ± 0.146
1.871MetPro: 1.871 ± 0.32
0.639MetGln: 0.639 ± 0.163
0.73MetArg: 0.73 ± 0.162
1.597MetSer: 1.597 ± 0.295
2.737MetThr: 2.737 ± 0.416
1.369MetVal: 1.369 ± 0.222
0.365MetTrp: 0.365 ± 0.139
0.319MetTyr: 0.319 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
3.924AsnAla: 3.924 ± 0.48
0.274AsnCys: 0.274 ± 0.11
2.007AsnAsp: 2.007 ± 0.298
1.186AsnGlu: 1.186 ± 0.222
0.593AsnPhe: 0.593 ± 0.168
3.011AsnGly: 3.011 ± 0.405
0.639AsnHis: 0.639 ± 0.176
1.141AsnIle: 1.141 ± 0.31
0.912AsnLys: 0.912 ± 0.2
2.19AsnLeu: 2.19 ± 0.32
0.456AsnMet: 0.456 ± 0.147
0.639AsnAsn: 0.639 ± 0.177
3.011AsnPro: 3.011 ± 0.399
0.411AsnGln: 0.411 ± 0.116
1.597AsnArg: 1.597 ± 0.231
1.141AsnSer: 1.141 ± 0.27
2.372AsnThr: 2.372 ± 0.301
2.281AsnVal: 2.281 ± 0.287
0.958AsnTrp: 0.958 ± 0.199
0.547AsnTyr: 0.547 ± 0.163
0.0AsnXaa: 0.0 ± 0.0
Pro
6.479ProAla: 6.479 ± 0.607
0.684ProCys: 0.684 ± 0.22
5.338ProAsp: 5.338 ± 0.584
5.612ProGlu: 5.612 ± 0.517
1.688ProPhe: 1.688 ± 0.257
5.794ProGly: 5.794 ± 0.538
0.502ProHis: 0.502 ± 0.127
3.102ProIle: 3.102 ± 0.347
1.46ProLys: 1.46 ± 0.249
3.376ProLeu: 3.376 ± 0.278
1.369ProMet: 1.369 ± 0.276
1.551ProAsn: 1.551 ± 0.358
4.517ProPro: 4.517 ± 0.552
1.186ProGln: 1.186 ± 0.219
3.057ProArg: 3.057 ± 0.329
3.467ProSer: 3.467 ± 0.428
3.832ProThr: 3.832 ± 0.392
5.566ProVal: 5.566 ± 0.555
1.232ProTrp: 1.232 ± 0.241
0.912ProTyr: 0.912 ± 0.227
0.0ProXaa: 0.0 ± 0.0
Gln
3.924GlnAla: 3.924 ± 0.385
0.456GlnCys: 0.456 ± 0.148
0.912GlnAsp: 0.912 ± 0.211
1.551GlnGlu: 1.551 ± 0.303
1.141GlnPhe: 1.141 ± 0.251
2.372GlnGly: 2.372 ± 0.371
0.593GlnHis: 0.593 ± 0.154
1.825GlnIle: 1.825 ± 0.331
0.912GlnLys: 0.912 ± 0.205
2.281GlnLeu: 2.281 ± 0.399
1.049GlnMet: 1.049 ± 0.2
0.547GlnAsn: 0.547 ± 0.155
1.688GlnPro: 1.688 ± 0.25
1.871GlnGln: 1.871 ± 0.261
2.327GlnArg: 2.327 ± 0.308
1.414GlnSer: 1.414 ± 0.259
1.734GlnThr: 1.734 ± 0.228
2.053GlnVal: 2.053 ± 0.321
0.639GlnTrp: 0.639 ± 0.153
1.095GlnTyr: 1.095 ± 0.196
0.0GlnXaa: 0.0 ± 0.0
Arg
6.661ArgAla: 6.661 ± 0.653
1.004ArgCys: 1.004 ± 0.234
4.836ArgAsp: 4.836 ± 0.502
4.106ArgGlu: 4.106 ± 0.533
1.962ArgPhe: 1.962 ± 0.302
4.927ArgGly: 4.927 ± 0.509
1.688ArgHis: 1.688 ± 0.313
2.966ArgIle: 2.966 ± 0.405
2.053ArgLys: 2.053 ± 0.302
5.521ArgLeu: 5.521 ± 0.521
1.688ArgMet: 1.688 ± 0.327
2.099ArgAsn: 2.099 ± 0.301
3.65ArgPro: 3.65 ± 0.436
2.646ArgGln: 2.646 ± 0.302
6.159ArgArg: 6.159 ± 0.848
3.878ArgSer: 3.878 ± 0.403
3.787ArgThr: 3.787 ± 0.417
5.019ArgVal: 5.019 ± 0.448
1.688ArgTrp: 1.688 ± 0.312
2.053ArgTyr: 2.053 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
6.251SerAla: 6.251 ± 0.433
0.502SerCys: 0.502 ± 0.162
3.376SerAsp: 3.376 ± 0.382
2.692SerGlu: 2.692 ± 0.375
1.46SerPhe: 1.46 ± 0.262
6.661SerGly: 6.661 ± 0.663
1.095SerHis: 1.095 ± 0.219
2.92SerIle: 2.92 ± 0.398
2.099SerLys: 2.099 ± 0.379
4.106SerLeu: 4.106 ± 0.48
0.867SerMet: 0.867 ± 0.226
1.642SerAsn: 1.642 ± 0.334
3.331SerPro: 3.331 ± 0.378
1.825SerGln: 1.825 ± 0.336
3.65SerArg: 3.65 ± 0.464
2.966SerSer: 2.966 ± 0.436
3.969SerThr: 3.969 ± 0.386
3.787SerVal: 3.787 ± 0.433
1.141SerTrp: 1.141 ± 0.294
0.867SerTyr: 0.867 ± 0.186
0.0SerXaa: 0.0 ± 0.0
Thr
7.984ThrAla: 7.984 ± 0.54
0.821ThrCys: 0.821 ± 0.251
3.832ThrAsp: 3.832 ± 0.378
3.376ThrGlu: 3.376 ± 0.373
1.871ThrPhe: 1.871 ± 0.213
6.935ThrGly: 6.935 ± 0.643
0.639ThrHis: 0.639 ± 0.209
3.239ThrIle: 3.239 ± 0.471
2.418ThrLys: 2.418 ± 0.27
5.475ThrLeu: 5.475 ± 0.452
1.277ThrMet: 1.277 ± 0.259
1.46ThrAsn: 1.46 ± 0.257
4.562ThrPro: 4.562 ± 0.47
1.825ThrGln: 1.825 ± 0.287
4.334ThrArg: 4.334 ± 0.482
3.331ThrSer: 3.331 ± 0.369
3.969ThrThr: 3.969 ± 0.425
5.84ThrVal: 5.84 ± 0.507
0.958ThrTrp: 0.958 ± 0.188
1.323ThrTyr: 1.323 ± 0.266
0.0ThrXaa: 0.0 ± 0.0
Val
8.623ValAla: 8.623 ± 0.614
0.73ValCys: 0.73 ± 0.217
5.612ValAsp: 5.612 ± 0.638
5.201ValGlu: 5.201 ± 0.435
1.642ValPhe: 1.642 ± 0.24
7.209ValGly: 7.209 ± 0.539
1.46ValHis: 1.46 ± 0.301
3.148ValIle: 3.148 ± 0.374
2.601ValLys: 2.601 ± 0.317
6.251ValLeu: 6.251 ± 0.466
1.414ValMet: 1.414 ± 0.26
3.011ValAsn: 3.011 ± 0.306
4.289ValPro: 4.289 ± 0.448
2.144ValGln: 2.144 ± 0.267
4.289ValArg: 4.289 ± 0.576
5.11ValSer: 5.11 ± 0.491
5.475ValThr: 5.475 ± 0.572
6.798ValVal: 6.798 ± 0.589
1.369ValTrp: 1.369 ± 0.263
1.962ValTyr: 1.962 ± 0.329
0.0ValXaa: 0.0 ± 0.0
Trp
1.734TrpAla: 1.734 ± 0.295
0.456TrpCys: 0.456 ± 0.162
1.369TrpAsp: 1.369 ± 0.248
1.369TrpGlu: 1.369 ± 0.21
0.547TrpPhe: 0.547 ± 0.189
0.958TrpGly: 0.958 ± 0.205
0.411TrpHis: 0.411 ± 0.15
0.912TrpIle: 0.912 ± 0.201
0.958TrpLys: 0.958 ± 0.214
1.506TrpLeu: 1.506 ± 0.288
0.365TrpMet: 0.365 ± 0.134
0.593TrpAsn: 0.593 ± 0.163
1.186TrpPro: 1.186 ± 0.251
0.502TrpGln: 0.502 ± 0.169
1.46TrpArg: 1.46 ± 0.275
1.506TrpSer: 1.506 ± 0.285
1.642TrpThr: 1.642 ± 0.301
1.095TrpVal: 1.095 ± 0.259
0.182TrpTrp: 0.182 ± 0.088
0.593TrpTyr: 0.593 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.19TyrAla: 2.19 ± 0.311
0.456TyrCys: 0.456 ± 0.15
1.688TyrAsp: 1.688 ± 0.287
1.141TyrGlu: 1.141 ± 0.166
0.73TyrPhe: 0.73 ± 0.177
2.053TyrGly: 2.053 ± 0.286
0.319TyrHis: 0.319 ± 0.124
0.776TyrIle: 0.776 ± 0.226
0.319TyrLys: 0.319 ± 0.108
2.418TyrLeu: 2.418 ± 0.406
0.365TyrMet: 0.365 ± 0.137
0.547TyrAsn: 0.547 ± 0.163
0.821TyrPro: 0.821 ± 0.192
1.004TyrGln: 1.004 ± 0.192
1.734TyrArg: 1.734 ± 0.331
1.049TyrSer: 1.049 ± 0.216
1.551TyrThr: 1.551 ± 0.289
2.19TyrVal: 2.19 ± 0.349
0.228TyrTrp: 0.228 ± 0.115
0.274TyrTyr: 0.274 ± 0.089
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (21919 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski