Yersinia phage phiR8-01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Pienvirus; Yersinia virus R8-01

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7K2I4|I7K2I4_9CAUD Uncharacterized protein OS=Yersinia phage phiR8-01 OX=1206556 GN=g025 PE=4 SV=2
MM1 pKa = 7.45RR2 pKa = 11.84HH3 pKa = 5.9ALINTVNVEE12 pKa = 4.27DD13 pKa = 4.29LCNAVPCGTMDD24 pKa = 3.75CWQCPLEE31 pKa = 4.21STQALNEE38 pKa = 4.03FLRR41 pKa = 11.84EE42 pKa = 4.14NTDD45 pKa = 3.24EE46 pKa = 4.06

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7K2H8|I7K2H8_9CAUD Uncharacterized protein OS=Yersinia phage phiR8-01 OX=1206556 GN=g015 PE=4 SV=2
MM1 pKa = 7.64SEE3 pKa = 3.86YY4 pKa = 11.05NKK6 pKa = 10.55AINARR11 pKa = 11.84KK12 pKa = 9.07EE13 pKa = 3.68RR14 pKa = 11.84QKK16 pKa = 10.89AIKK19 pKa = 9.75RR20 pKa = 11.84ARR22 pKa = 11.84KK23 pKa = 8.11EE24 pKa = 4.05KK25 pKa = 10.5EE26 pKa = 3.53GMRR29 pKa = 11.84YY30 pKa = 5.51TTEE33 pKa = 3.87GDD35 pKa = 3.69FTHH38 pKa = 7.27LASNTMKK45 pKa = 10.24FRR47 pKa = 11.84RR48 pKa = 11.84GG49 pKa = 3.12

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

13104

36

1253

262.1

29.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.501 ± 0.501

1.007 ± 0.163

6.334 ± 0.228

6.227 ± 0.323

3.671 ± 0.219

7.578 ± 0.338

1.908 ± 0.138

4.51 ± 0.215

6.143 ± 0.246

8.15 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.365 ± 0.171

4.464 ± 0.228

4.075 ± 0.228

4.327 ± 0.328

5.693 ± 0.269

5.624 ± 0.339

5.678 ± 0.249

6.7 ± 0.302

1.633 ± 0.22

3.404 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski