Escherichia phage FFH2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Vequintavirus; Escherichia virus FFH2

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023MHU1|A0A023MHU1_9CAUD Uncharacterized protein OS=Escherichia phage FFH2 OX=1446490 PE=4 SV=1
MM1 pKa = 7.0NTFTLGFTDD10 pKa = 4.93RR11 pKa = 11.84EE12 pKa = 4.27DD13 pKa = 4.13FNGFMDD19 pKa = 5.38EE20 pKa = 3.78IGFYY24 pKa = 10.73EE25 pKa = 4.97NEE27 pKa = 4.05VLQNTLLVEE36 pKa = 4.34VVGFIYY42 pKa = 10.51TEE44 pKa = 3.9TGDD47 pKa = 3.45TYY49 pKa = 10.84EE50 pKa = 5.9AEE52 pKa = 4.5DD53 pKa = 3.73EE54 pKa = 4.42FGNMVVYY61 pKa = 7.91QVCEE65 pKa = 3.96KK66 pKa = 11.17SEE68 pKa = 3.94LFYY71 pKa = 11.72VNVLALDD78 pKa = 3.82EE79 pKa = 4.48DD80 pKa = 4.27AEE82 pKa = 4.62NIVSKK87 pKa = 10.43SGKK90 pKa = 9.38VVDD93 pKa = 5.17PIRR96 pKa = 11.84LNMFDD101 pKa = 4.3PSVSTLEE108 pKa = 4.06ALL110 pKa = 4.08

Molecular weight: 12.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023MHW2|A0A023MHW2_9CAUD Uncharacterized protein OS=Escherichia phage FFH2 OX=1446490 PE=4 SV=1
MM1 pKa = 7.16NAYY4 pKa = 9.55GIKK7 pKa = 9.73VRR9 pKa = 11.84RR10 pKa = 11.84NARR13 pKa = 11.84QVVTDD18 pKa = 3.48QMVCQVDD25 pKa = 3.64VTINNGQGLYY35 pKa = 10.37KK36 pKa = 10.48FRR38 pKa = 11.84RR39 pKa = 11.84GQSYY43 pKa = 8.3TRR45 pKa = 11.84HH46 pKa = 4.98QLNRR50 pKa = 11.84CALTKK55 pKa = 10.63GQINHH60 pKa = 6.33WFNKK64 pKa = 9.06KK65 pKa = 9.61

Molecular weight: 7.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

218

0

218

41817

37

1875

191.8

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.983 ± 0.213

1.648 ± 0.088

6.78 ± 0.149

7.086 ± 0.29

4.266 ± 0.095

7.059 ± 0.22

1.968 ± 0.085

5.955 ± 0.149

6.842 ± 0.232

7.688 ± 0.199

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.86 ± 0.102

4.998 ± 0.17

3.812 ± 0.119

3.192 ± 0.115

4.579 ± 0.12

5.488 ± 0.143

5.684 ± 0.223

7.055 ± 0.149

1.817 ± 0.088

4.24 ± 0.12
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski