Eggplant mottled dwarf nucleorhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140KP54|A0A140KP54_9RHAB Phosphoprotein OS=Eggplant mottled dwarf nucleorhabdovirus OX=488317 GN=P PE=4 SV=1
MM1 pKa = 7.88SEE3 pKa = 4.0TTTNRR8 pKa = 11.84PITPDD13 pKa = 3.26EE14 pKa = 4.59ANDD17 pKa = 3.73DD18 pKa = 4.19VFVEE22 pKa = 4.39MCRR25 pKa = 11.84MVDD28 pKa = 3.33NLLSQSEE35 pKa = 4.7TNKK38 pKa = 10.13HH39 pKa = 6.38DD40 pKa = 4.22ANQDD44 pKa = 3.83SIPITIEE51 pKa = 3.8EE52 pKa = 4.31EE53 pKa = 4.01TSDD56 pKa = 4.96AEE58 pKa = 5.28SSDD61 pKa = 2.58SWTYY65 pKa = 11.63YY66 pKa = 11.08DD67 pKa = 3.74FTDD70 pKa = 4.22GRR72 pKa = 11.84DD73 pKa = 3.36YY74 pKa = 11.47YY75 pKa = 11.67YY76 pKa = 10.92DD77 pKa = 5.27DD78 pKa = 4.68NDD80 pKa = 3.88SDD82 pKa = 3.83IYY84 pKa = 11.43EE85 pKa = 4.53LMAEE89 pKa = 4.52CNHH92 pKa = 6.34GEE94 pKa = 3.94WGNN97 pKa = 3.42

Molecular weight: 11.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140KP58|A0A140KP58_9RHAB Large structural protein OS=Eggplant mottled dwarf nucleorhabdovirus OX=488317 GN=G PE=4 SV=1
MM1 pKa = 7.45INVIGRR7 pKa = 11.84SSASVQGIYY16 pKa = 10.28DD17 pKa = 3.56VEE19 pKa = 4.17KK20 pKa = 11.07DD21 pKa = 3.63KK22 pKa = 11.73VSLLSTVVGQPISLNYY38 pKa = 9.84IITIHH43 pKa = 5.97MYY45 pKa = 8.57EE46 pKa = 4.6AEE48 pKa = 4.09MKK50 pKa = 10.41KK51 pKa = 10.43AFEE54 pKa = 4.3EE55 pKa = 5.19GEE57 pKa = 4.18LHH59 pKa = 5.76YY60 pKa = 10.86HH61 pKa = 7.52DD62 pKa = 4.98IFDD65 pKa = 4.5MIEE68 pKa = 4.03SALEE72 pKa = 4.07LPSTRR77 pKa = 11.84PDD79 pKa = 3.16AVKK82 pKa = 10.37RR83 pKa = 11.84SPPNMTARR91 pKa = 11.84VAKK94 pKa = 10.43NILQICRR101 pKa = 11.84IHH103 pKa = 7.53AIHH106 pKa = 6.74TNAKK110 pKa = 7.93MVFMNTSTDD119 pKa = 3.22HH120 pKa = 6.91SNEE123 pKa = 3.96TPTLVIKK130 pKa = 10.62EE131 pKa = 4.27GEE133 pKa = 4.32TLVSQNYY140 pKa = 8.97DD141 pKa = 3.24SHH143 pKa = 7.93VVEE146 pKa = 5.28IVNQAGDD153 pKa = 3.66NPLSGSYY160 pKa = 10.41HH161 pKa = 5.65LTLEE165 pKa = 4.19KK166 pKa = 10.35PYY168 pKa = 10.09ISSDD172 pKa = 3.16SRR174 pKa = 11.84IVGHH178 pKa = 6.93LSFAAYY184 pKa = 9.66VRR186 pKa = 11.84APPRR190 pKa = 11.84GQAATRR196 pKa = 11.84TRR198 pKa = 11.84LNIRR202 pKa = 11.84VLASKK207 pKa = 8.29YY208 pKa = 8.15QRR210 pKa = 11.84NASQKK215 pKa = 9.4VNSLFRR221 pKa = 11.84KK222 pKa = 8.87SIKK225 pKa = 10.27RR226 pKa = 11.84KK227 pKa = 10.19GDD229 pKa = 4.24DD230 pKa = 3.14ISKK233 pKa = 9.89KK234 pKa = 8.3PHH236 pKa = 6.23SSGIMDD242 pKa = 4.57AVAKK246 pKa = 10.03ILKK249 pKa = 10.29SSS251 pKa = 3.27

Molecular weight: 27.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

3966

97

1946

566.6

63.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.018 ± 1.022

1.664 ± 0.45

6.43 ± 0.365

5.522 ± 0.465

3.404 ± 0.355

5.673 ± 0.297

2.37 ± 0.436

7.892 ± 0.286

5.547 ± 0.626

8.447 ± 0.856

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.833 ± 0.352

4.639 ± 0.417

4.791 ± 0.6

3.127 ± 0.283

5.169 ± 0.811

9.178 ± 0.898

6.051 ± 0.467

6.203 ± 0.286

1.16 ± 0.302

3.883 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski