Kushneria phosphatilytica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Kushneria

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S1NXX3|A0A1S1NXX3_9GAMM Ribose-phosphate pyrophosphokinase OS=Kushneria phosphatilytica OX=657387 GN=prs PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 9.06KK3 pKa = 8.27TLLATAIAGVLGTLATGAQAATVYY27 pKa = 10.61NQDD30 pKa = 2.98GSKK33 pKa = 10.71LDD35 pKa = 3.46VYY37 pKa = 11.56GNVQLAFRR45 pKa = 11.84SIEE48 pKa = 4.37GYY50 pKa = 10.55DD51 pKa = 4.11DD52 pKa = 5.19SDD54 pKa = 3.75DD55 pKa = 4.08TSDD58 pKa = 4.49HH59 pKa = 5.97GTDD62 pKa = 3.32SYY64 pKa = 12.15SDD66 pKa = 3.86LFDD69 pKa = 3.98NGSTIGFRR77 pKa = 11.84GEE79 pKa = 4.66HH80 pKa = 6.77IINPGLTGYY89 pKa = 9.99FRR91 pKa = 11.84AEE93 pKa = 3.87FEE95 pKa = 4.8FNADD99 pKa = 3.51QQKK102 pKa = 10.91GYY104 pKa = 9.75YY105 pKa = 9.05AAYY108 pKa = 10.0DD109 pKa = 3.77NPDD112 pKa = 3.56DD113 pKa = 4.58QSFTEE118 pKa = 4.37SGLSRR123 pKa = 11.84GDD125 pKa = 3.32QAYY128 pKa = 10.86LGLTGNFGDD137 pKa = 4.98LRR139 pKa = 11.84IGSWDD144 pKa = 3.58SLFDD148 pKa = 3.88DD149 pKa = 5.66WIADD153 pKa = 3.99PISNNEE159 pKa = 3.88YY160 pKa = 10.66FDD162 pKa = 3.75NTDD165 pKa = 3.04AQSDD169 pKa = 4.13VGGTAYY175 pKa = 10.08EE176 pKa = 4.19SDD178 pKa = 3.87KK179 pKa = 10.45ITYY182 pKa = 7.55TSPIFSGLQFVVGTQYY198 pKa = 11.34KK199 pKa = 10.85GDD201 pKa = 3.77GEE203 pKa = 5.44DD204 pKa = 3.64YY205 pKa = 11.02NQYY208 pKa = 11.2DD209 pKa = 3.69YY210 pKa = 11.82DD211 pKa = 3.9AATGAGEE218 pKa = 5.08FVLDD222 pKa = 4.72DD223 pKa = 4.93DD224 pKa = 4.99NSASFFGGVRR234 pKa = 11.84YY235 pKa = 8.21TMAGWTLAAVYY246 pKa = 10.7DD247 pKa = 4.0DD248 pKa = 5.08LNNFEE253 pKa = 5.0YY254 pKa = 10.41ISAAGNEE261 pKa = 4.02HH262 pKa = 7.62DD263 pKa = 4.35FGKK266 pKa = 10.84LYY268 pKa = 10.66GANLSYY274 pKa = 10.72QWDD277 pKa = 4.14TLRR280 pKa = 11.84VAAKK284 pKa = 9.46WGHH287 pKa = 6.0QDD289 pKa = 4.7AKK291 pKa = 10.29WSDD294 pKa = 3.6DD295 pKa = 3.58ADD297 pKa = 3.32VDD299 pKa = 4.57RR300 pKa = 11.84YY301 pKa = 11.3GLGARR306 pKa = 11.84WGYY309 pKa = 11.51GNGDD313 pKa = 3.69LYY315 pKa = 11.5GSYY318 pKa = 10.39QYY320 pKa = 11.69VDD322 pKa = 3.69AEE324 pKa = 4.19GDD326 pKa = 3.35AAALIDD332 pKa = 3.78PRR334 pKa = 11.84RR335 pKa = 11.84FNDD338 pKa = 3.82DD339 pKa = 3.33NGNTSDD345 pKa = 3.13QHH347 pKa = 7.15YY348 pKa = 11.21NEE350 pKa = 5.58FILGATYY357 pKa = 10.91NLSSQMYY364 pKa = 9.59VFLEE368 pKa = 4.16GAKK371 pKa = 10.06YY372 pKa = 10.09DD373 pKa = 3.77RR374 pKa = 11.84KK375 pKa = 11.18DD376 pKa = 3.54NIGDD380 pKa = 3.89GVSTGMVYY388 pKa = 10.61SFF390 pKa = 4.61

Molecular weight: 42.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S1NNV1|A0A1S1NNV1_9GAMM MFS domain-containing protein OS=Kushneria phosphatilytica OX=657387 GN=BH688_13075 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.5RR12 pKa = 11.84KK13 pKa = 9.44RR14 pKa = 11.84NHH16 pKa = 5.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.32AGRR28 pKa = 11.84QILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3055

0

3055

948163

26

1612

310.4

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.503 ± 0.053

0.993 ± 0.014

5.731 ± 0.04

6.263 ± 0.05

3.418 ± 0.03

8.088 ± 0.041

2.767 ± 0.027

5.064 ± 0.041

2.239 ± 0.03

11.166 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.633 ± 0.022

2.704 ± 0.026

4.822 ± 0.031

4.18 ± 0.039

7.735 ± 0.046

5.769 ± 0.032

5.242 ± 0.028

6.741 ± 0.038

1.567 ± 0.021

2.377 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski