Ageratum yellow vein virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9DXI1|Q9DXI1_9GEMI Transcriptional activator protein OS=Ageratum yellow vein virus OX=44560 GN=C2 PE=3 SV=1
MM1 pKa = 7.43PRR3 pKa = 11.84SSSFQVKK10 pKa = 9.88AKK12 pKa = 10.67NIFLTYY18 pKa = 8.78PKK20 pKa = 10.43CPIPKK25 pKa = 9.95EE26 pKa = 3.91EE27 pKa = 4.07ALEE30 pKa = 4.45LIKK33 pKa = 10.8NIQCPSDD40 pKa = 3.23KK41 pKa = 10.97LFIRR45 pKa = 11.84VAQEE49 pKa = 3.53KK50 pKa = 10.53HH51 pKa = 6.26SDD53 pKa = 3.63GTLHH57 pKa = 6.79LHH59 pKa = 7.02ALIQFKK65 pKa = 10.86GKK67 pKa = 10.02AQFRR71 pKa = 11.84NSRR74 pKa = 11.84HH75 pKa = 5.67FDD77 pKa = 2.8ITHH80 pKa = 7.38PNTSTQFHH88 pKa = 7.33PNFQGAKK95 pKa = 9.08SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.8IEE105 pKa = 4.11KK106 pKa = 10.91DD107 pKa = 2.87GDD109 pKa = 3.93YY110 pKa = 11.1VDD112 pKa = 3.8WGVFQIDD119 pKa = 3.23GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSKK143 pKa = 8.46EE144 pKa = 3.51AAMAIIRR151 pKa = 11.84EE152 pKa = 4.31KK153 pKa = 10.9LPKK156 pKa = 9.83EE157 pKa = 4.29YY158 pKa = 10.15IFQFHH163 pKa = 6.73NLNTNLDD170 pKa = 4.21RR171 pKa = 11.84IFAPPLEE178 pKa = 4.42VFVCPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.95EE195 pKa = 4.04LEE197 pKa = 3.96EE198 pKa = 4.17WVSEE202 pKa = 4.26NVSGAAARR210 pKa = 11.84PWRR213 pKa = 11.84PKK215 pKa = 10.37SIVIEE220 pKa = 4.41GEE222 pKa = 3.95SRR224 pKa = 11.84TGKK227 pKa = 8.58TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 11.03KK312 pKa = 10.55EE313 pKa = 3.94YY314 pKa = 10.94LDD316 pKa = 3.87EE317 pKa = 4.82EE318 pKa = 4.68KK319 pKa = 11.13NSALKK324 pKa = 10.19EE325 pKa = 3.96WAIKK329 pKa = 9.9NAEE332 pKa = 4.05FVTLNNPLYY341 pKa = 10.97SGTNQSAAQNSEE353 pKa = 4.29EE354 pKa = 4.32EE355 pKa = 4.42ASSQAA360 pKa = 3.47

Molecular weight: 40.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65315|Q65315_9GEMI Protein V2 OS=Ageratum yellow vein virus OX=44560 GN=V1 PE=3 SV=1
MM1 pKa = 7.47GLCISTRR8 pKa = 11.84SYY10 pKa = 10.95NSKK13 pKa = 10.13VKK15 pKa = 9.84PNSGIVDD22 pKa = 4.28TSTLHH27 pKa = 6.47TPTPPHH33 pKa = 6.57NSIQTSRR40 pKa = 11.84EE41 pKa = 3.81LSPVPMSSPTSRR53 pKa = 11.84RR54 pKa = 11.84TVITSTGVYY63 pKa = 9.66FRR65 pKa = 11.84STEE68 pKa = 3.95DD69 pKa = 3.25LLEE72 pKa = 4.27EE73 pKa = 4.23VNRR76 pKa = 11.84RR77 pKa = 11.84LTMQLQKK84 pKa = 10.87RR85 pKa = 3.94

Molecular weight: 9.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1087

85

360

181.2

20.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.336 ± 1.143

2.392 ± 0.596

4.876 ± 0.395

4.876 ± 0.973

4.232 ± 0.547

4.232 ± 0.517

3.956 ± 0.767

5.612 ± 0.879

5.704 ± 1.061

6.716 ± 0.474

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.547

6.164 ± 0.512

5.888 ± 0.629

5.152 ± 0.853

7.084 ± 1.288

7.452 ± 1.028

6.532 ± 0.998

6.348 ± 1.051

1.38 ± 0.314

3.864 ± 0.681
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski