Gordonia phage Bunnybear

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nymbaxtervirinae; Nymphadoravirus; unclassified Nymphadoravirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LLJ6|A0A7G8LLJ6_9CAUD Uncharacterized protein OS=Gordonia phage Bunnybear OX=2762398 GN=62 PE=4 SV=1
MM1 pKa = 7.52ACEE4 pKa = 4.82QEE6 pKa = 5.64LCANWDD12 pKa = 4.18GIGCPCAYY20 pKa = 10.58LDD22 pKa = 3.58TDD24 pKa = 3.39EE25 pKa = 5.74DD26 pKa = 3.94EE27 pKa = 5.25RR28 pKa = 11.84PVVCGYY34 pKa = 10.25CQTLLTVCAEE44 pKa = 4.17RR45 pKa = 11.84LCCPNCTHH53 pKa = 7.93PYY55 pKa = 9.34PGGPNGTGGDD65 pKa = 3.48RR66 pKa = 3.65

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LLE4|A0A7G8LLE4_9CAUD Minor capsid protein OS=Gordonia phage Bunnybear OX=2762398 GN=10 PE=4 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84APLTRR7 pKa = 11.84SRR9 pKa = 11.84LSMADD14 pKa = 3.17LLARR18 pKa = 11.84FFTQPFTVRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 8.31TGDD32 pKa = 3.39GAVGPVYY39 pKa = 10.95AEE41 pKa = 3.91LVTLHH46 pKa = 6.18GRR48 pKa = 11.84INSTNRR54 pKa = 11.84LVIDD58 pKa = 3.7DD59 pKa = 4.91RR60 pKa = 11.84GNQVLSAAKK69 pKa = 9.23ISMSITEE76 pKa = 4.1GRR78 pKa = 11.84IPTGSQVRR86 pKa = 11.84IDD88 pKa = 3.97DD89 pKa = 4.47GPWRR93 pKa = 11.84TVIAEE98 pKa = 4.04SRR100 pKa = 11.84HH101 pKa = 4.74VGGFRR106 pKa = 11.84KK107 pKa = 10.35SPDD110 pKa = 3.47YY111 pKa = 11.31YY112 pKa = 11.38SIDD115 pKa = 3.74LNN117 pKa = 3.96

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

16767

24

2142

215.0

23.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.535 ± 0.591

0.883 ± 0.162

7.115 ± 0.403

5.648 ± 0.219

2.642 ± 0.154

8.558 ± 0.392

2.117 ± 0.228

4.503 ± 0.184

3.197 ± 0.232

7.658 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.129 ± 0.137

2.833 ± 0.195

5.964 ± 0.263

3.531 ± 0.326

7.819 ± 0.458

5.231 ± 0.202

7.145 ± 0.262

7.336 ± 0.214

1.891 ± 0.16

2.266 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski