Rhizobium sp. LC145

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3BKE4|A0A0M3BKE4_9RHIZ Uncharacterized protein OS=Rhizobium sp. LC145 OX=1120688 GN=YH62_11370 PE=4 SV=1
MM1 pKa = 6.78TTMDD5 pKa = 5.32KK6 pKa = 9.23ITEE9 pKa = 4.59EE10 pKa = 3.69ISHH13 pKa = 7.0FIGLFHH19 pKa = 6.01TTIEE23 pKa = 4.13DD24 pKa = 3.23VRR26 pKa = 11.84LRR28 pKa = 11.84EE29 pKa = 4.13AYY31 pKa = 9.39TDD33 pKa = 3.75FTYY36 pKa = 10.45HH37 pKa = 6.06VPQHH41 pKa = 5.94HH42 pKa = 6.67LRR44 pKa = 11.84VEE46 pKa = 4.09PVFDD50 pKa = 4.64SNFEE54 pKa = 4.04APFEE58 pKa = 4.02FLGFDD63 pKa = 3.36PGVSYY68 pKa = 10.6KK69 pKa = 10.85APAYY73 pKa = 8.86VKK75 pKa = 10.23PITHH79 pKa = 7.29PAFLSHH85 pKa = 6.55IQHH88 pKa = 6.64PPIPVVDD95 pKa = 4.92GPSEE99 pKa = 4.2YY100 pKa = 10.14PGMDD104 pKa = 3.81LPRR107 pKa = 11.84DD108 pKa = 3.93LGPAGPRR115 pKa = 11.84SMSVTLEE122 pKa = 3.52IDD124 pKa = 3.5PPGSVANYY132 pKa = 7.14MVQAAGLSDD141 pKa = 4.52DD142 pKa = 5.59DD143 pKa = 4.18YY144 pKa = 11.78FNVGGSGLAFNPEE157 pKa = 4.24PVNDD161 pKa = 3.98TDD163 pKa = 4.49LLEE166 pKa = 4.27AAEE169 pKa = 5.59DD170 pKa = 3.73ILSLSPIGDD179 pKa = 3.52PEE181 pKa = 4.26MPGSGDD187 pKa = 3.46EE188 pKa = 4.39LIEE191 pKa = 4.66LIEE194 pKa = 4.59TIASQLKK201 pKa = 9.02NWTASAEE208 pKa = 4.25SPMEE212 pKa = 4.14VFVHH216 pKa = 5.67QAATIEE222 pKa = 4.58GIYY225 pKa = 10.76VNGEE229 pKa = 4.16LVTEE233 pKa = 4.26APKK236 pKa = 10.94LSDD239 pKa = 3.16YY240 pKa = 11.31HH241 pKa = 8.58SFDD244 pKa = 4.99DD245 pKa = 5.21EE246 pKa = 4.85EE247 pKa = 5.44EE248 pKa = 5.13GDD250 pKa = 5.05DD251 pKa = 4.89DD252 pKa = 4.87TDD254 pKa = 5.24DD255 pKa = 4.41GPAPNVVVHH264 pKa = 7.07ADD266 pKa = 3.41GTVTIDD272 pKa = 3.24VSVEE276 pKa = 3.89VVAGGNTVVNNAVMQNLWTAATVTAVLGDD305 pKa = 3.7HH306 pKa = 6.69FEE308 pKa = 4.32INAIIQTNAIWDD320 pKa = 3.72TDD322 pKa = 4.34AITSAIEE329 pKa = 3.7SWTNSAGANEE339 pKa = 4.54IFNIATFEE347 pKa = 4.1RR348 pKa = 11.84MDD350 pKa = 3.47SSEE353 pKa = 4.23DD354 pKa = 3.17TGAQPVTGGFPSAWAITEE372 pKa = 3.94IQGDD376 pKa = 3.95LMIANWLEE384 pKa = 3.7QYY386 pKa = 10.76IFVSDD391 pKa = 3.69NDD393 pKa = 3.48IGILSSSGVTTSVIAGDD410 pKa = 3.72NTSTNHH416 pKa = 5.22VSLYY420 pKa = 10.55EE421 pKa = 4.22LGLSYY426 pKa = 11.29DD427 pKa = 4.49LIIVGGSVYY436 pKa = 10.41DD437 pKa = 3.82ANIIHH442 pKa = 6.0QFNLLFDD449 pKa = 4.39NDD451 pKa = 3.59VVGAVSGFEE460 pKa = 4.23TTGEE464 pKa = 4.33GAFSGAGNLLWNQAYY479 pKa = 9.54IYY481 pKa = 10.2NIGGADD487 pKa = 3.72RR488 pKa = 11.84FDD490 pKa = 4.84ALPSAYY496 pKa = 10.22LDD498 pKa = 3.7AANGIAAGRR507 pKa = 11.84NSLPNGVLTDD517 pKa = 3.74PAFGGMEE524 pKa = 4.35GLRR527 pKa = 11.84VLYY530 pKa = 10.36ISGDD534 pKa = 3.74LLNIQYY540 pKa = 9.76IRR542 pKa = 11.84QTTIVGDD549 pKa = 3.95DD550 pKa = 3.77DD551 pKa = 4.94QIALAMNAVNPHH563 pKa = 7.1PDD565 pKa = 3.24ASWSVSTGDD574 pKa = 3.25NTLVNTAAILDD585 pKa = 4.36LDD587 pKa = 4.41SMGKK591 pKa = 8.03TYY593 pKa = 11.0VGGEE597 pKa = 4.09QYY599 pKa = 10.88SQEE602 pKa = 4.08TLIQAEE608 pKa = 5.19LISSDD613 pKa = 3.88PEE615 pKa = 3.84LAARR619 pKa = 11.84DD620 pKa = 3.75PNEE623 pKa = 4.12LVNEE627 pKa = 4.1AVVFLDD633 pKa = 5.08DD634 pKa = 4.84SMLTAEE640 pKa = 4.83TDD642 pKa = 3.68AQGLPAPEE650 pKa = 5.02HH651 pKa = 6.78DD652 pKa = 3.64SHH654 pKa = 6.41YY655 pKa = 11.15QNDD658 pKa = 4.36GLQHH662 pKa = 5.34VLGG665 pKa = 4.52

Molecular weight: 71.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3BRG3|A0A0M3BRG3_9RHIZ GntR family transcriptional regulator OS=Rhizobium sp. LC145 OX=1120688 GN=YH62_07460 PE=3 SV=1
MM1 pKa = 7.02MMAMLTRR8 pKa = 11.84MRR10 pKa = 11.84MPTQTQTLMRR20 pKa = 11.84MLTPMQTPMRR30 pKa = 11.84MRR32 pKa = 11.84TLMPMLMPTRR42 pKa = 11.84TQMLMPMPMRR52 pKa = 11.84MPTLMQMLMPMPMRR66 pKa = 11.84MPTLMQMQMLTLMQMLTPTLMRR88 pKa = 11.84MPTQTLTPMLMRR100 pKa = 11.84MLTLMQMRR108 pKa = 11.84MRR110 pKa = 11.84MPTLMRR116 pKa = 11.84MRR118 pKa = 11.84MLTPMRR124 pKa = 11.84MPTPTQMLMRR134 pKa = 11.84MPTQTQTLTPTLTRR148 pKa = 11.84MQTPTQMLMRR158 pKa = 11.84MPTLMPMRR166 pKa = 11.84TQMLMQMLTPTLMRR180 pKa = 11.84MPTQTLTPMLMRR192 pKa = 11.84TLMQMQMLTRR202 pKa = 11.84MQTPMQMLMRR212 pKa = 11.84MPTLMRR218 pKa = 11.84MPMLMPMPMPTRR230 pKa = 11.84TPMRR234 pKa = 11.84MRR236 pKa = 11.84TQMPTQTLMPMPMLMRR252 pKa = 11.84TQMQMLTPMRR262 pKa = 11.84MQTPTLMPMLMLMQMRR278 pKa = 11.84MPTLMPMLTLTPMRR292 pKa = 11.84MPTLTPMRR300 pKa = 11.84MPTLTPMRR308 pKa = 11.84MPTRR312 pKa = 11.84TLTPMRR318 pKa = 11.84TPMQTPMRR326 pKa = 11.84TQMPMPMPTLMQTLTPMRR344 pKa = 11.84MRR346 pKa = 11.84MLTLTPMRR354 pKa = 11.84MPTLTPMRR362 pKa = 11.84MPTRR366 pKa = 11.84TPMRR370 pKa = 11.84TLMRR374 pKa = 11.84MRR376 pKa = 11.84MLLMILLPRR385 pKa = 11.84RR386 pKa = 11.84LSSAPSPARR395 pKa = 11.84EE396 pKa = 4.27QISPPTPHH404 pKa = 6.53SS405 pKa = 3.46

Molecular weight: 48.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5603

0

5603

1692521

28

2835

302.1

32.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.851 ± 0.044

0.747 ± 0.011

5.455 ± 0.025

6.194 ± 0.035

3.972 ± 0.023

8.396 ± 0.03

1.981 ± 0.016

5.739 ± 0.023

3.619 ± 0.029

9.983 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.017

2.754 ± 0.018

4.985 ± 0.023

3.028 ± 0.019

6.856 ± 0.037

5.672 ± 0.019

5.207 ± 0.024

7.301 ± 0.028

1.303 ± 0.013

2.313 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski