Synechococcus phage syn9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 226 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0QZJ7|Q0QZJ7_BPSYS Gp31 OS=Synechococcus phage syn9 OX=382359 PE=4 SV=1
MM1 pKa = 7.77AKK3 pKa = 9.54TGLLAQSKK11 pKa = 8.98PAAATNTVLYY21 pKa = 9.23KK22 pKa = 10.95APIGNSASAVLTVANDD38 pKa = 4.12GTGSAYY44 pKa = 10.88SLALKK49 pKa = 10.45DD50 pKa = 3.34WDD52 pKa = 4.21QKK54 pKa = 10.53LTLDD58 pKa = 3.64ANSYY62 pKa = 10.0LLHH65 pKa = 6.9PGDD68 pKa = 4.37VITAYY73 pKa = 10.57RR74 pKa = 11.84IDD76 pKa = 3.74VDD78 pKa = 3.14TDD80 pKa = 3.22MNANSGFTSGLALTSGDD97 pKa = 3.84EE98 pKa = 4.07EE99 pKa = 4.69KK100 pKa = 11.1VFQFEE105 pKa = 4.37SFYY108 pKa = 11.47VPAFTEE114 pKa = 3.99IFVKK118 pKa = 10.59DD119 pKa = 3.36VALRR123 pKa = 11.84QVTIEE128 pKa = 4.08SVTGNFEE135 pKa = 3.98VGQTLTTGTSPDD147 pKa = 3.53DD148 pKa = 3.56TEE150 pKa = 4.19ATVFAVDD157 pKa = 3.44DD158 pKa = 4.09TGANTILYY166 pKa = 9.28IGPSTINGTGAEE178 pKa = 4.23FTDD181 pKa = 3.92GDD183 pKa = 3.9IVATAFGGSATISAGGIAAAVNAFVFSTTTAGGVYY218 pKa = 10.02DD219 pKa = 3.79YY220 pKa = 11.0AQNGLEE226 pKa = 3.96IFADD230 pKa = 3.44RR231 pKa = 11.84AYY233 pKa = 10.78RR234 pKa = 11.84FDD236 pKa = 3.86VSDD239 pKa = 3.41SSMTGRR245 pKa = 11.84DD246 pKa = 3.47FKK248 pKa = 11.5LSITANGEE256 pKa = 3.74WGPDD260 pKa = 3.37GVIGGDD266 pKa = 3.51PSDD269 pKa = 5.39DD270 pKa = 3.35GTEE273 pKa = 3.94YY274 pKa = 8.06TTNKK278 pKa = 8.76TINGTAGSASAYY290 pKa = 8.74VQYY293 pKa = 11.2DD294 pKa = 3.45LAGTGITGSLYY305 pKa = 10.48FYY307 pKa = 11.02DD308 pKa = 4.9GGTGTAGNSIYY319 pKa = 10.87GGTSRR324 pKa = 11.84VLSTSNQYY332 pKa = 9.8TYY334 pKa = 11.03TGMYY338 pKa = 9.08VYY340 pKa = 10.58NVNGDD345 pKa = 3.7LSGGTVTFTHH355 pKa = 6.75NDD357 pKa = 2.73VTYY360 pKa = 9.41TIDD363 pKa = 3.4SVTVGAYY370 pKa = 10.05GYY372 pKa = 9.51VRR374 pKa = 11.84SYY376 pKa = 11.17VGTEE380 pKa = 3.56LRR382 pKa = 11.84VCLGAGSTDD391 pKa = 3.64FAGTNTFRR399 pKa = 11.84DD400 pKa = 3.69NPISQTATRR409 pKa = 11.84NTATVSSVDD418 pKa = 3.39AGSADD423 pKa = 3.47VDD425 pKa = 3.69NEE427 pKa = 4.32SYY429 pKa = 11.17LIISKK434 pKa = 9.2TNAANNIDD442 pKa = 3.94RR443 pKa = 11.84TTSLVVGPGEE453 pKa = 3.97KK454 pKa = 10.23LIVSSTTQNNVFNLVGFEE472 pKa = 4.24DD473 pKa = 4.19VSTSFTTRR481 pKa = 11.84VNVADD486 pKa = 3.9YY487 pKa = 10.76GGAGAGDD494 pKa = 3.92GGGGVAPP501 pKa = 4.93

Molecular weight: 51.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0QZE5|Q0QZE5_BPSYS Gp83 OS=Synechococcus phage syn9 OX=382359 PE=4 SV=1
MM1 pKa = 7.52PVIKK5 pKa = 10.6VSQRR9 pKa = 11.84PFQMPLDD16 pKa = 3.64RR17 pKa = 11.84VFYY20 pKa = 9.74RR21 pKa = 11.84SGRR24 pKa = 11.84EE25 pKa = 3.54AHH27 pKa = 6.16LTHH30 pKa = 6.94SKK32 pKa = 10.29PSLIASHH39 pKa = 6.77AA40 pKa = 3.76

Molecular weight: 4.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

226

0

226

57766

39

6076

255.6

28.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.854 ± 0.229

0.973 ± 0.095

6.734 ± 0.135

6.062 ± 0.252

4.364 ± 0.117

7.877 ± 0.323

1.648 ± 0.134

6.369 ± 0.221

5.749 ± 0.417

7.129 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.205

5.92 ± 0.19

4.072 ± 0.139

3.698 ± 0.087

4.075 ± 0.129

6.862 ± 0.185

7.311 ± 0.377

6.597 ± 0.19

1.245 ± 0.094

4.307 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski