Yersinia phage phiR1-RT

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tegunavirus; Yersinia virus R1RT

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7J3Y7|I7J3Y7_9CAUD Phage tail sheath monomer OS=Yersinia phage phiR1-RT OX=1206558 GN=g169 PE=3 SV=1
MM1 pKa = 7.29SVAIFLKK8 pKa = 10.67SEE10 pKa = 3.83SGDD13 pKa = 3.51NYY15 pKa = 10.64LYY17 pKa = 10.87SYY19 pKa = 10.95DD20 pKa = 4.02ADD22 pKa = 4.0KK23 pKa = 10.62STSDD27 pKa = 4.59IEE29 pKa = 4.21YY30 pKa = 10.47ALQNEE35 pKa = 4.01ISYY38 pKa = 10.05IGKK41 pKa = 9.23IADD44 pKa = 3.59FEE46 pKa = 4.58TATGDD51 pKa = 3.31NCSEE55 pKa = 3.9EE56 pKa = 4.9RR57 pKa = 11.84EE58 pKa = 4.29TAIHH62 pKa = 7.24DD63 pKa = 3.8IVSKK67 pKa = 10.88LRR69 pKa = 11.84DD70 pKa = 3.57QSWEE74 pKa = 3.66SDD76 pKa = 3.63YY77 pKa = 11.88EE78 pKa = 3.98EE79 pKa = 5.99DD80 pKa = 4.06YY81 pKa = 11.36EE82 pKa = 5.5SSEE85 pKa = 4.82DD86 pKa = 3.76YY87 pKa = 11.32

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7LEN5|I7LEN5_9CAUD Capsid vertex protein OS=Yersinia phage phiR1-RT OX=1206558 GN=g178 PE=3 SV=1
MM1 pKa = 7.64LIISDD6 pKa = 3.89DD7 pKa = 3.83HH8 pKa = 6.17EE9 pKa = 4.14LVYY12 pKa = 11.16EE13 pKa = 4.15NVQALIPEE21 pKa = 4.27AQNRR25 pKa = 11.84INFLATFSKK34 pKa = 11.32DD35 pKa = 2.97EE36 pKa = 3.86ITAITEE42 pKa = 4.07NMVDD46 pKa = 3.73TTPDD50 pKa = 3.2LAIAIASLNEE60 pKa = 3.72EE61 pKa = 4.05MEE63 pKa = 4.35LNEE66 pKa = 5.06FIVKK70 pKa = 9.74HH71 pKa = 4.74VSSRR75 pKa = 11.84GEE77 pKa = 3.72VTKK80 pKa = 9.71THH82 pKa = 6.27DD83 pKa = 3.06RR84 pKa = 11.84KK85 pKa = 8.5TRR87 pKa = 11.84SRR89 pKa = 11.84RR90 pKa = 11.84AFQTTGLSKK99 pKa = 10.77AKK101 pKa = 9.95RR102 pKa = 11.84RR103 pKa = 11.84AIARR107 pKa = 11.84KK108 pKa = 7.78VVRR111 pKa = 11.84SKK113 pKa = 11.03KK114 pKa = 9.41SNPSATVRR122 pKa = 11.84GSRR125 pKa = 11.84KK126 pKa = 9.22RR127 pKa = 11.84KK128 pKa = 8.63RR129 pKa = 11.84AMKK132 pKa = 9.66RR133 pKa = 11.84RR134 pKa = 11.84KK135 pKa = 9.81IMGLSS140 pKa = 3.21

Molecular weight: 15.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

262

0

262

51568

38

1245

196.8

22.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.553 ± 0.178

1.117 ± 0.068

6.362 ± 0.109

6.762 ± 0.162

4.315 ± 0.097

5.726 ± 0.177

1.821 ± 0.08

7.755 ± 0.133

7.541 ± 0.217

7.827 ± 0.133

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.676 ± 0.086

5.827 ± 0.125

3.318 ± 0.101

3.403 ± 0.107

4.127 ± 0.089

6.964 ± 0.125

6.054 ± 0.216

6.264 ± 0.126

1.321 ± 0.061

4.268 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski