Mycobacterium sp. 852013-51886_SCH5428379

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4393 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A0TCC2|A0A1A0TCC2_9MYCO Glycosyl transferase OS=Mycobacterium sp. 852013-51886_SCH5428379 OX=1834111 GN=A5757_03170 PE=4 SV=1
MM1 pKa = 7.73LLTLTACGSDD11 pKa = 4.38DD12 pKa = 3.49EE13 pKa = 4.67TAAAGPNSTVTPVASLAAAAVDD35 pKa = 4.14DD36 pKa = 4.43LQAYY40 pKa = 8.81WSEE43 pKa = 4.17QFPALYY49 pKa = 10.1GSEE52 pKa = 4.09YY53 pKa = 10.83VPLKK57 pKa = 10.2GGVYY61 pKa = 10.62ALTASSTKK69 pKa = 9.66WPEE72 pKa = 3.85CATEE76 pKa = 4.01YY77 pKa = 11.47ADD79 pKa = 4.03VEE81 pKa = 4.63GNAFFCQLDD90 pKa = 4.2DD91 pKa = 4.01SVSWDD96 pKa = 3.55AEE98 pKa = 4.0GLLPEE103 pKa = 4.59LQANFGDD110 pKa = 4.19FVIPVVLAHH119 pKa = 6.31EE120 pKa = 4.52WGHH123 pKa = 5.82AVQHH127 pKa = 6.33RR128 pKa = 11.84SGFFDD133 pKa = 3.62EE134 pKa = 4.77NALTVSSEE142 pKa = 3.96LQADD146 pKa = 4.58CFAGAWSRR154 pKa = 11.84HH155 pKa = 4.59ARR157 pKa = 11.84DD158 pKa = 2.96SGLFQVSDD166 pKa = 4.06DD167 pKa = 4.06QLDD170 pKa = 3.75DD171 pKa = 3.97ALAGVLDD178 pKa = 4.46LRR180 pKa = 11.84DD181 pKa = 3.74APGTSAEE188 pKa = 4.69DD189 pKa = 3.95PSAHH193 pKa = 6.18GSGFDD198 pKa = 3.73RR199 pKa = 11.84VAAFQDD205 pKa = 4.44GYY207 pKa = 11.41DD208 pKa = 3.73NAAGRR213 pKa = 11.84CKK215 pKa = 10.04EE216 pKa = 3.98YY217 pKa = 10.56RR218 pKa = 11.84DD219 pKa = 4.0GEE221 pKa = 4.36PTVLEE226 pKa = 4.69LPFEE230 pKa = 4.61DD231 pKa = 4.41AEE233 pKa = 4.36EE234 pKa = 4.13AASEE238 pKa = 4.34GNAPYY243 pKa = 10.74DD244 pKa = 3.79SIVNGVPYY252 pKa = 10.51DD253 pKa = 3.99LEE255 pKa = 5.44DD256 pKa = 3.69YY257 pKa = 7.47WTHH260 pKa = 6.06VYY262 pKa = 10.62PEE264 pKa = 4.07ITGGKK269 pKa = 7.89PWVPLRR275 pKa = 11.84GLEE278 pKa = 4.26PFDD281 pKa = 3.99PANPPMCDD289 pKa = 3.11GEE291 pKa = 6.07ADD293 pKa = 3.57DD294 pKa = 6.47DD295 pKa = 4.22YY296 pKa = 11.73TLFYY300 pKa = 10.5CVPDD304 pKa = 5.72DD305 pKa = 4.62FVAWDD310 pKa = 3.83NVEE313 pKa = 4.96DD314 pKa = 3.77MPEE317 pKa = 3.97VYY319 pKa = 10.04RR320 pKa = 11.84EE321 pKa = 4.17GGDD324 pKa = 3.33WAVGTLLATQYY335 pKa = 11.46GLAALTRR342 pKa = 11.84MGDD345 pKa = 3.29QSDD348 pKa = 4.23DD349 pKa = 3.47RR350 pKa = 11.84TSTLRR355 pKa = 11.84SDD357 pKa = 4.42CLAGSYY363 pKa = 7.66TASVMLFNRR372 pKa = 11.84ADD374 pKa = 3.39TSSFRR379 pKa = 11.84ISPGDD384 pKa = 3.54LDD386 pKa = 4.05EE387 pKa = 6.4GITALLVFRR396 pKa = 11.84GDD398 pKa = 3.21GDD400 pKa = 3.78VEE402 pKa = 4.28RR403 pKa = 11.84QGHH406 pKa = 5.1GFSRR410 pKa = 11.84VRR412 pKa = 11.84AYY414 pKa = 10.76RR415 pKa = 11.84EE416 pKa = 3.96GVLNGAEE423 pKa = 4.33GCLTYY428 pKa = 10.7QPP430 pKa = 4.68

Molecular weight: 46.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A0TBW8|A0A1A0TBW8_9MYCO Xaa-Pro aminopeptidase OS=Mycobacterium sp. 852013-51886_SCH5428379 OX=1834111 GN=A5757_02230 PE=4 SV=1
MM1 pKa = 7.81ASTPNMSTPPVMIAIDD17 pKa = 3.4PHH19 pKa = 6.46KK20 pKa = 10.61RR21 pKa = 11.84SWTAAAVDD29 pKa = 4.01VRR31 pKa = 11.84LQPLATIRR39 pKa = 11.84VPVSRR44 pKa = 11.84DD45 pKa = 3.0GYY47 pKa = 8.86RR48 pKa = 11.84TLLRR52 pKa = 11.84FARR55 pKa = 11.84RR56 pKa = 11.84WPDD59 pKa = 3.25TAWAIEE65 pKa = 4.17GASGLGAPLTVRR77 pKa = 11.84LRR79 pKa = 11.84QDD81 pKa = 4.14GITVLDD87 pKa = 4.21VPAKK91 pKa = 9.35LAARR95 pKa = 11.84VRR97 pKa = 11.84LLSTGHH103 pKa = 5.72GRR105 pKa = 11.84KK106 pKa = 9.21SDD108 pKa = 3.47EE109 pKa = 3.97ADD111 pKa = 3.19AVSVGVAAHH120 pKa = 6.41TSSSLNTAEE129 pKa = 4.66IDD131 pKa = 3.74SATTALRR138 pKa = 11.84ALVEE142 pKa = 4.15HH143 pKa = 7.25RR144 pKa = 11.84DD145 pKa = 3.86DD146 pKa = 3.86LVKK149 pKa = 10.26TRR151 pKa = 11.84TQTINRR157 pKa = 11.84LHH159 pKa = 6.11TVLTHH164 pKa = 6.8LIPGGASRR172 pKa = 11.84DD173 pKa = 3.81LNAEE177 pKa = 3.78RR178 pKa = 11.84AAEE181 pKa = 4.03LLRR184 pKa = 11.84TVRR187 pKa = 11.84PRR189 pKa = 11.84DD190 pKa = 3.46TAGKK194 pKa = 7.11TLRR197 pKa = 11.84ALAADD202 pKa = 5.57LISEE206 pKa = 4.21LRR208 pKa = 11.84GLDD211 pKa = 3.28RR212 pKa = 11.84RR213 pKa = 11.84IDD215 pKa = 3.7KK216 pKa = 10.39AVHH219 pKa = 6.62DD220 pKa = 4.23IEE222 pKa = 5.55IAVKK226 pKa = 10.65DD227 pKa = 3.87CGTTLTEE234 pKa = 3.77LHH236 pKa = 6.89GIGALTAAKK245 pKa = 9.44IVSRR249 pKa = 11.84VGSIQRR255 pKa = 11.84FDD257 pKa = 3.52SPAAFASYY265 pKa = 9.87TGTAPIEE272 pKa = 4.21VSSGDD277 pKa = 3.71VVRR280 pKa = 11.84HH281 pKa = 5.25RR282 pKa = 11.84LSRR285 pKa = 11.84AGDD288 pKa = 3.46RR289 pKa = 11.84QLNYY293 pKa = 9.25CLHH296 pKa = 6.66VMAITQIRR304 pKa = 11.84RR305 pKa = 11.84DD306 pKa = 3.82TPGRR310 pKa = 11.84TYY312 pKa = 11.11YY313 pKa = 10.33LRR315 pKa = 11.84KK316 pKa = 9.02RR317 pKa = 11.84ANGKK321 pKa = 8.19SHH323 pKa = 6.54SEE325 pKa = 3.53AMRR328 pKa = 11.84CLKK331 pKa = 10.35RR332 pKa = 11.84RR333 pKa = 11.84LSDD336 pKa = 3.16TVYY339 pKa = 10.67RR340 pKa = 11.84QLLRR344 pKa = 11.84DD345 pKa = 3.73ANTTTT350 pKa = 3.72

Molecular weight: 38.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4393

0

4393

1448496

48

4179

329.7

35.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.28 ± 0.051

0.766 ± 0.009

6.529 ± 0.036

5.285 ± 0.034

3.026 ± 0.02

9.088 ± 0.094

2.139 ± 0.018

4.135 ± 0.024

1.962 ± 0.029

9.834 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.036 ± 0.016

2.082 ± 0.022

5.834 ± 0.037

2.852 ± 0.02

7.415 ± 0.041

5.159 ± 0.025

6.115 ± 0.03

8.894 ± 0.032

1.482 ± 0.016

2.086 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski