Streptococcus satellite phage Javan309

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJA6|A0A4D5ZJA6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan309 OX=2558627 GN=JavanS309_0011 PE=4 SV=1
MM1 pKa = 7.74IYY3 pKa = 10.14SKK5 pKa = 10.8EE6 pKa = 3.99IVRR9 pKa = 11.84EE10 pKa = 3.93WLDD13 pKa = 3.73EE14 pKa = 4.04VAEE17 pKa = 4.16RR18 pKa = 11.84AKK20 pKa = 10.79DD21 pKa = 3.42HH22 pKa = 6.92PEE24 pKa = 3.57WVDD27 pKa = 3.29VFEE30 pKa = 4.78RR31 pKa = 11.84CYY33 pKa = 10.93TDD35 pKa = 3.42TLDD38 pKa = 3.54NTVEE42 pKa = 3.94ILEE45 pKa = 4.88DD46 pKa = 3.64GSTFVLTGDD55 pKa = 4.14IPAMWLRR62 pKa = 11.84DD63 pKa = 3.32STAQLRR69 pKa = 11.84PYY71 pKa = 10.38LHH73 pKa = 5.85VAKK76 pKa = 10.12RR77 pKa = 11.84DD78 pKa = 3.42PLLRR82 pKa = 11.84QTIAGLVKK90 pKa = 10.4RR91 pKa = 11.84QMTLILKK98 pKa = 10.09DD99 pKa = 3.66PYY101 pKa = 11.29ANSFNIEE108 pKa = 4.17EE109 pKa = 4.14NWKK112 pKa = 9.1GHH114 pKa = 6.42HH115 pKa = 5.69EE116 pKa = 4.16TDD118 pKa = 3.49HH119 pKa = 6.69TDD121 pKa = 3.16LNGWIWEE128 pKa = 4.15RR129 pKa = 11.84KK130 pKa = 9.0YY131 pKa = 10.94EE132 pKa = 4.16VDD134 pKa = 3.59SLCYY138 pKa = 9.51PLQLAYY144 pKa = 10.73LLWKK148 pKa = 8.5EE149 pKa = 4.32TGEE152 pKa = 4.0TSQFDD157 pKa = 3.91EE158 pKa = 4.66TFVAATKK165 pKa = 10.31EE166 pKa = 4.09ILHH169 pKa = 6.71LWTVEE174 pKa = 3.83QDD176 pKa = 3.86HH177 pKa = 6.81KK178 pKa = 10.74NSPYY182 pKa = 10.66RR183 pKa = 11.84FVRR186 pKa = 11.84DD187 pKa = 3.34TDD189 pKa = 3.58RR190 pKa = 11.84KK191 pKa = 10.6EE192 pKa = 3.82DD193 pKa = 3.61TLVNDD198 pKa = 4.14GFGPNFAVTGMTWSAFRR215 pKa = 11.84PSDD218 pKa = 3.51DD219 pKa = 3.59CCQYY223 pKa = 11.51SYY225 pKa = 11.01LIPSNMFAVVVLGYY239 pKa = 9.29VQEE242 pKa = 4.52IFAEE246 pKa = 4.29LDD248 pKa = 3.46LADD251 pKa = 4.14SQSVTTDD258 pKa = 2.75AKK260 pKa = 10.58RR261 pKa = 11.84LQSEE265 pKa = 3.97IQEE268 pKa = 4.64GIEE271 pKa = 3.9NYY273 pKa = 10.71AYY275 pKa = 9.5TSNSKK280 pKa = 10.75GEE282 pKa = 4.04KK283 pKa = 9.27IYY285 pKa = 11.05AFEE288 pKa = 4.42VDD290 pKa = 3.53GLGNASIMDD299 pKa = 4.24DD300 pKa = 3.79PNVPSLLAAPYY311 pKa = 10.21LGYY314 pKa = 10.7CSVDD318 pKa = 3.41DD319 pKa = 4.34EE320 pKa = 5.33VYY322 pKa = 9.84QATRR326 pKa = 11.84RR327 pKa = 11.84TILSPEE333 pKa = 3.71NPYY336 pKa = 10.25FYY338 pKa = 10.23QGEE341 pKa = 4.37YY342 pKa = 10.97ASGLGSSHH350 pKa = 5.8TFYY353 pKa = 10.98RR354 pKa = 11.84YY355 pKa = 8.88IWPIALSIQGLTTRR369 pKa = 11.84DD370 pKa = 3.3KK371 pKa = 11.55AEE373 pKa = 4.21KK374 pKa = 10.44KK375 pKa = 10.4FLLDD379 pKa = 3.92QLVACDD385 pKa = 3.54GGTGVMHH392 pKa = 7.19EE393 pKa = 4.79SFHH396 pKa = 8.56VDD398 pKa = 3.77DD399 pKa = 4.03PTLYY403 pKa = 10.06SRR405 pKa = 11.84EE406 pKa = 4.11WFSWANMMFCEE417 pKa = 5.34LVLDD421 pKa = 4.09YY422 pKa = 11.56LDD424 pKa = 3.63IRR426 pKa = 4.46

Molecular weight: 49.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZJ29|A0A4D5ZJ29_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan309 OX=2558627 GN=JavanS309_0013 PE=4 SV=1
MM1 pKa = 7.56FDD3 pKa = 4.24SFPEE7 pKa = 3.89RR8 pKa = 11.84PKK10 pKa = 10.37YY11 pKa = 9.41MKK13 pKa = 9.51RR14 pKa = 11.84DD15 pKa = 3.72RR16 pKa = 11.84YY17 pKa = 9.01HH18 pKa = 4.93KK19 pKa = 10.08HH20 pKa = 4.07YY21 pKa = 10.89RR22 pKa = 11.84KK23 pKa = 9.39FLKK26 pKa = 9.0YY27 pKa = 8.31TKK29 pKa = 10.35KK30 pKa = 10.73GDD32 pKa = 3.79RR33 pKa = 11.84FWLNGSRR40 pKa = 11.84LL41 pKa = 3.57

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

3441

41

498

191.2

22.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.754 ± 0.248

0.639 ± 0.185

5.987 ± 0.494

8.253 ± 0.609

4.33 ± 0.222

5.202 ± 0.391

2.209 ± 0.255

6.51 ± 0.575

8.457 ± 0.659

9.59 ± 0.494

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.291

4.999 ± 0.525

3.371 ± 0.463

3.981 ± 0.384

5.144 ± 0.341

5.812 ± 0.274

6.074 ± 0.452

5.086 ± 0.356

1.162 ± 0.228

4.795 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski