Pseudooceanicola sp. GBMRC 2024

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudooceanicola; unclassified Pseudooceanicola

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L7FYJ2|A0A6L7FYJ2_9RHOB Metal-dependent carboxypeptidase OS=Pseudooceanicola sp. GBMRC 2024 OX=2692189 GN=GR170_01005 PE=3 SV=1
MM1 pKa = 7.11KK2 pKa = 10.02TIFLTSAFALAAGAAAAGSYY22 pKa = 10.47GPAKK26 pKa = 9.81MEE28 pKa = 4.37PAPMVATPVMAAPTADD44 pKa = 2.74WTGPYY49 pKa = 9.86LGVAAGVGKK58 pKa = 10.77VEE60 pKa = 4.22ADD62 pKa = 3.49YY63 pKa = 10.98MGTTDD68 pKa = 6.36DD69 pKa = 4.61EE70 pKa = 4.6DD71 pKa = 4.33LNAYY75 pKa = 9.44GVFGGYY81 pKa = 10.28DD82 pKa = 3.45YY83 pKa = 12.01DD84 pKa = 3.5MGQWVLGGEE93 pKa = 4.35LDD95 pKa = 4.0YY96 pKa = 11.9NSIDD100 pKa = 4.62IDD102 pKa = 3.82NTDD105 pKa = 4.58DD106 pKa = 5.84DD107 pKa = 4.9GDD109 pKa = 3.97LYY111 pKa = 11.08RR112 pKa = 11.84LRR114 pKa = 11.84GRR116 pKa = 11.84IGYY119 pKa = 8.85DD120 pKa = 2.54AGAFMPYY127 pKa = 8.5VTLGAAWLKK136 pKa = 11.0VDD138 pKa = 5.52DD139 pKa = 4.29EE140 pKa = 4.62TDD142 pKa = 2.93NGFTYY147 pKa = 10.55GAGVDD152 pKa = 3.62YY153 pKa = 9.69MINEE157 pKa = 4.15NFSIGAEE164 pKa = 3.47ITMNSFRR171 pKa = 11.84DD172 pKa = 4.0FQDD175 pKa = 3.04TSGLDD180 pKa = 2.95VDD182 pKa = 4.91ARR184 pKa = 11.84MIQLRR189 pKa = 11.84GSFRR193 pKa = 11.84FF194 pKa = 3.59

Molecular weight: 20.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L7G3R5|A0A6L7G3R5_9RHOB Exodeoxyribonuclease 7 large subunit OS=Pseudooceanicola sp. GBMRC 2024 OX=2692189 GN=xseA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5050

0

5050

1626961

22

10194

322.2

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.037 ± 0.05

0.907 ± 0.011

5.61 ± 0.039

5.675 ± 0.031

3.566 ± 0.021

8.929 ± 0.039

2.038 ± 0.016

4.837 ± 0.023

2.768 ± 0.03

10.823 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.021

2.255 ± 0.021

5.535 ± 0.038

3.209 ± 0.019

7.091 ± 0.045

5.088 ± 0.029

5.358 ± 0.034

7.029 ± 0.03

1.36 ± 0.016

2.133 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski