Ailuropoda melanoleuca (Giant panda)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1MB88|G1MB88_AILME Isoform of A0A7N5KHU5 Retinol binding protein 1 OS=Ailuropoda melanoleuca OX=9646 GN=RBP1 PE=3 SV=1
MM1 pKa = 7.29EE2 pKa = 4.36VVVLIPYY9 pKa = 10.0VLGLLLLPLLAVALCVRR26 pKa = 11.84CRR28 pKa = 11.84EE29 pKa = 4.18MPGSYY34 pKa = 8.87DD35 pKa = 3.14TGASDD40 pKa = 4.39SLTPSIVLKK49 pKa = 10.57RR50 pKa = 11.84PPTVVPWPSATSYY63 pKa = 11.6APVTSYY69 pKa = 11.21PPLSQPDD76 pKa = 4.23LLPIPRR82 pKa = 11.84SPQPHH87 pKa = 6.67RR88 pKa = 11.84MPSSRR93 pKa = 11.84QDD95 pKa = 2.92SDD97 pKa = 3.47GANSVASYY105 pKa = 10.76EE106 pKa = 4.13NEE108 pKa = 3.97EE109 pKa = 4.44PVCEE113 pKa = 5.48DD114 pKa = 3.98DD115 pKa = 6.7DD116 pKa = 4.74EE117 pKa = 7.03DD118 pKa = 4.69EE119 pKa = 4.31EE120 pKa = 5.84DD121 pKa = 3.83YY122 pKa = 11.55HH123 pKa = 8.47NEE125 pKa = 4.01GYY127 pKa = 11.01LEE129 pKa = 4.22VLPDD133 pKa = 3.73STPTTSTAVAPAPVPSNPGLRR154 pKa = 11.84DD155 pKa = 3.14SAFSMEE161 pKa = 4.23SGEE164 pKa = 4.92DD165 pKa = 3.63YY166 pKa = 11.71VNVAEE171 pKa = 4.7SEE173 pKa = 4.17EE174 pKa = 4.28SANVSLDD181 pKa = 3.14GSRR184 pKa = 11.84EE185 pKa = 3.96YY186 pKa = 11.72VNVSQEE192 pKa = 4.44LPPMARR198 pKa = 11.84TQPAILNSQKK208 pKa = 10.21VEE210 pKa = 4.2DD211 pKa = 4.43EE212 pKa = 4.21DD213 pKa = 5.59DD214 pKa = 3.99EE215 pKa = 4.77EE216 pKa = 5.11EE217 pKa = 4.32EE218 pKa = 4.72GAPDD222 pKa = 3.77YY223 pKa = 11.52EE224 pKa = 4.36NLQGLHH230 pKa = 6.14

Molecular weight: 24.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7N5KIS3|A0A7N5KIS3_AILME RB binding protein 8 endonuclease OS=Ailuropoda melanoleuca OX=9646 GN=RBBP8 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20764

17764

38528

22681218

21

8798

588.7

65.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.026 ± 0.013

2.174 ± 0.011

4.825 ± 0.009

7.136 ± 0.018

3.595 ± 0.009

6.577 ± 0.017

2.556 ± 0.006

4.289 ± 0.011

5.756 ± 0.016

9.872 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.005

3.579 ± 0.009

6.348 ± 0.021

4.779 ± 0.013

5.763 ± 0.011

8.369 ± 0.017

5.259 ± 0.009

6.031 ± 0.01

1.196 ± 0.004

2.608 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski