Lactobacillus saerimneri 30a

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Ligilactobacillus; Ligilactobacillus saerimneri

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1519 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M5J4F0|M5J4F0_9LACO Polar amino acid ABC transporter permease protein OS=Lactobacillus saerimneri 30a OX=1227363 GN=D271_03210 PE=3 SV=1
MM1 pKa = 8.18RR2 pKa = 11.84EE3 pKa = 3.92RR4 pKa = 11.84NDD6 pKa = 3.44EE7 pKa = 4.47LFNCTNFALYY17 pKa = 10.52CSFYY21 pKa = 11.05SVDD24 pKa = 3.31EE25 pKa = 4.16ATIKK29 pKa = 10.39EE30 pKa = 4.3DD31 pKa = 3.72PVVADD36 pKa = 3.3YY37 pKa = 11.13VAAHH41 pKa = 6.71RR42 pKa = 11.84EE43 pKa = 3.99ALEE46 pKa = 3.9ALIAGYY52 pKa = 11.15GEE54 pKa = 4.14MGNLNQEE61 pKa = 3.39ICAEE65 pKa = 4.07YY66 pKa = 10.67LGIDD70 pKa = 3.75CPGIGDD76 pKa = 4.83LSDD79 pKa = 3.3KK80 pKa = 11.22

Molecular weight: 8.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M5J4L8|M5J4L8_9LACO Uncharacterized protein OS=Lactobacillus saerimneri 30a OX=1227363 GN=D271_02819 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.13RR4 pKa = 11.84TFQPKK9 pKa = 8.63KK10 pKa = 7.79RR11 pKa = 11.84HH12 pKa = 4.83RR13 pKa = 11.84QRR15 pKa = 11.84VHH17 pKa = 6.07GFRR20 pKa = 11.84KK21 pKa = 10.03RR22 pKa = 11.84MSTSNGRR29 pKa = 11.84KK30 pKa = 8.13VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1519

0

1519

471763

37

2750

310.6

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.681 ± 0.075

0.562 ± 0.017

5.703 ± 0.064

5.575 ± 0.058

4.054 ± 0.05

6.74 ± 0.06

2.208 ± 0.03

6.646 ± 0.064

6.079 ± 0.07

9.702 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.032

4.452 ± 0.049

3.751 ± 0.037

5.104 ± 0.071

4.487 ± 0.056

5.106 ± 0.057

6.347 ± 0.073

7.378 ± 0.057

1.056 ± 0.024

3.713 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski