Sphingomonas sp. YR710

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4037 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6V8A7|A0A1G6V8A7_9SPHN Uncharacterized protein OS=Sphingomonas sp. YR710 OX=1882773 GN=SAMN05444678_11463 PE=4 SV=1
MM1 pKa = 7.12ATSAPVIYY9 pKa = 10.72DD10 pKa = 3.32GDD12 pKa = 3.89LSDD15 pKa = 3.68WSDD18 pKa = 3.71SEE20 pKa = 5.63RR21 pKa = 11.84IDD23 pKa = 3.99NAIEE27 pKa = 4.19SPPGYY32 pKa = 10.53ALYY35 pKa = 10.59ARR37 pKa = 11.84PDD39 pKa = 3.54GSSLAFAFHH48 pKa = 6.33SAVPIGANTTFWLNTDD64 pKa = 3.41RR65 pKa = 11.84DD66 pKa = 3.94ATTGYY71 pKa = 10.59QIFGFAGGAEE81 pKa = 4.27YY82 pKa = 10.78NVNIAADD89 pKa = 4.29GTASLYY95 pKa = 10.34TGAAGQTLVLANLQIAYY112 pKa = 10.04NADD115 pKa = 3.12HH116 pKa = 6.37TVAEE120 pKa = 4.75FSLPKK125 pKa = 10.17AAIGNPPAIDD135 pKa = 3.27VLYY138 pKa = 10.51DD139 pKa = 3.29VNDD142 pKa = 3.63AVFGPTSYY150 pKa = 11.0SDD152 pKa = 3.75KK153 pKa = 10.95PFTLFNDD160 pKa = 3.55TGIAPNLSTKK170 pKa = 10.57VGIVYY175 pKa = 10.34SDD177 pKa = 3.55TTAHH181 pKa = 6.18QYY183 pKa = 10.55FSEE186 pKa = 4.17TAYY189 pKa = 10.77AQLFMNAQAQAMQAGVSFDD208 pKa = 4.31LLSEE212 pKa = 4.29SDD214 pKa = 3.76LTDD217 pKa = 3.69LSKK220 pKa = 10.66ISQYY224 pKa = 10.03DD225 pKa = 3.46TLIFPSFRR233 pKa = 11.84NVQASQANAIANTLQQAEE251 pKa = 4.43VQFGVGLIVGGEE263 pKa = 4.0FMTDD267 pKa = 3.4DD268 pKa = 4.37EE269 pKa = 5.22NNNPLPGDD277 pKa = 3.6SYY279 pKa = 12.15SRR281 pKa = 11.84MKK283 pKa = 10.92LLLDD287 pKa = 3.48ATRR290 pKa = 11.84VTGGTGDD297 pKa = 3.65VSLQATDD304 pKa = 3.44PGGLVLQNYY313 pKa = 9.28ASNEE317 pKa = 3.96VIRR320 pKa = 11.84NYY322 pKa = 10.53TGVGWNAFTNVSGTGTTVATEE343 pKa = 4.44TIGGQTYY350 pKa = 9.43AAAMATQTGGRR361 pKa = 11.84NVVFSSEE368 pKa = 3.48ATMADD373 pKa = 5.18SNLLWQAIDD382 pKa = 3.78YY383 pKa = 8.47SAQGTGVSVGLQLTRR398 pKa = 11.84QTGIVAARR406 pKa = 11.84VDD408 pKa = 4.91LDD410 pKa = 3.34SSMYY414 pKa = 11.07AEE416 pKa = 5.3DD417 pKa = 4.19VNPLDD422 pKa = 4.54GSPGVYY428 pKa = 10.13DD429 pKa = 3.56KK430 pKa = 11.23LVPILAQWKK439 pKa = 9.21DD440 pKa = 2.98AYY442 pKa = 11.22NFVGSLYY449 pKa = 11.15ANIGNNPAQGIYY461 pKa = 10.12TDD463 pKa = 3.59WTVSLPYY470 pKa = 9.43YY471 pKa = 10.46KK472 pKa = 11.21AMIDD476 pKa = 3.57LGNEE480 pKa = 3.99IGTHH484 pKa = 6.22SYY486 pKa = 7.96THH488 pKa = 7.45PDD490 pKa = 3.09NTNLLSAAQIQTEE503 pKa = 4.45FADD506 pKa = 3.4STTILNQQLSAYY518 pKa = 9.73LGHH521 pKa = 7.74PYY523 pKa = 10.34NIVGAAVPGAPEE535 pKa = 3.88TVATSTEE542 pKa = 3.38IMKK545 pKa = 10.09YY546 pKa = 7.58VQQYY550 pKa = 7.98LTGGYY555 pKa = 9.14SGVGAGYY562 pKa = 9.45PDD564 pKa = 3.57AFGFLSPTQEE574 pKa = 3.86NKK576 pKa = 10.22VYY578 pKa = 10.45LAPNTSFDD586 pKa = 3.47FTLVEE591 pKa = 4.45FQGKK595 pKa = 8.13TIAQAEE601 pKa = 4.32AAWAQEE607 pKa = 3.98WTDD610 pKa = 3.9LTSKK614 pKa = 10.42GQTPIVVWPFHH625 pKa = 7.41DD626 pKa = 3.82YY627 pKa = 10.6AAAAFDD633 pKa = 4.02VNGTGTSPYY642 pKa = 6.39TTQMFTDD649 pKa = 4.86WIARR653 pKa = 11.84ASASGSEE660 pKa = 4.27FVTLADD666 pKa = 3.82LADD669 pKa = 5.6RR670 pKa = 11.84IQAFDD675 pKa = 3.51QASVTTSVVGNTITATVGSSDD696 pKa = 3.4AGRR699 pKa = 11.84FALDD703 pKa = 3.6LQRR706 pKa = 11.84QGSSVIQNVDD716 pKa = 1.94NWYY719 pKa = 10.47AYY721 pKa = 8.58DD722 pKa = 3.65TDD724 pKa = 5.01SIFMPASGGTFTIHH738 pKa = 7.03LGAAQDD744 pKa = 4.12DD745 pKa = 4.44VTHH748 pKa = 6.8IAALPMRR755 pKa = 11.84ASLISVAGDD764 pKa = 3.25GRR766 pKa = 11.84NLAFSVEE773 pKa = 3.97GDD775 pKa = 3.63GLVTVDD781 pKa = 4.42LQNPGTDD788 pKa = 4.38LINVTGATIASQSGEE803 pKa = 4.03ILTLNLGAAGHH814 pKa = 6.69HH815 pKa = 6.94DD816 pKa = 3.65VTIGLGADD824 pKa = 3.49SAPVITSNGGGDD836 pKa = 3.51TAALSIPEE844 pKa = 4.73HH845 pKa = 6.07IAAVATVVATDD856 pKa = 4.08ANAGDD861 pKa = 3.29ITTYY865 pKa = 10.93GIASGVDD872 pKa = 3.08ASLFAIDD879 pKa = 4.38SVTGVLSFKK888 pKa = 10.35VAPDD892 pKa = 3.22YY893 pKa = 9.52TLPQDD898 pKa = 3.63SDD900 pKa = 3.97HH901 pKa = 6.55NNSYY905 pKa = 10.51LVNVSATDD913 pKa = 3.38SHH915 pKa = 6.93GLADD919 pKa = 4.45LQQLTVNVTNANVAPIITSNGAGATATLGIAEE951 pKa = 4.46NTTAVTMVVATDD963 pKa = 3.62ANRR966 pKa = 11.84ATGDD970 pKa = 3.29TTTYY974 pKa = 10.62GIVSGADD981 pKa = 2.98AALFAIDD988 pKa = 4.47PVTGVLTFKK997 pKa = 10.7AAPDD1001 pKa = 3.59YY1002 pKa = 9.98EE1003 pKa = 4.44LPQDD1007 pKa = 3.66SDD1009 pKa = 3.96HH1010 pKa = 6.57NNSYY1014 pKa = 10.6LVNISATDD1022 pKa = 3.47SRR1024 pKa = 11.84GLADD1028 pKa = 4.37LQNLTVNVTNVLGIVQTGTSGNDD1051 pKa = 2.5IMTGTIEE1058 pKa = 4.83PDD1060 pKa = 3.44TLSGAGGADD1069 pKa = 3.77TISGLGGNDD1078 pKa = 2.89ILNGNAGKK1086 pKa = 10.21DD1087 pKa = 3.5IINGGVGNDD1096 pKa = 4.2RR1097 pKa = 11.84ITGGAGADD1105 pKa = 3.75TLTGGPGNDD1114 pKa = 2.93LFVYY1118 pKa = 10.28TDD1120 pKa = 3.44VADD1123 pKa = 4.05SGTTATTRR1131 pKa = 11.84DD1132 pKa = 3.55VITDD1136 pKa = 3.96FEE1138 pKa = 4.38HH1139 pKa = 7.45AIDD1142 pKa = 5.68RR1143 pKa = 11.84IDD1145 pKa = 4.98LSAIDD1150 pKa = 4.15ANAGRR1155 pKa = 11.84GSLRR1159 pKa = 11.84GDD1161 pKa = 3.22QAFTFLPVAGTAFTGAAQLRR1181 pKa = 11.84YY1182 pKa = 8.29TYY1184 pKa = 9.26VTVGGVEE1191 pKa = 3.91HH1192 pKa = 6.91TIVEE1196 pKa = 4.09GDD1198 pKa = 3.66VNVNHH1203 pKa = 7.07IGADD1207 pKa = 3.67FSIDD1211 pKa = 3.68LVGHH1215 pKa = 6.27QILTASDD1222 pKa = 4.95FILL1225 pKa = 4.96

Molecular weight: 127.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6I3Z6|A0A1G6I3Z6_9SPHN Uncharacterized conserved protein DUF885 familyt OS=Sphingomonas sp. YR710 OX=1882773 GN=SAMN05444678_101111 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.53GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.45LSAA44 pKa = 3.94

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4037

0

4037

1304086

28

5710

323.0

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.313 ± 0.06

0.791 ± 0.011

6.026 ± 0.035

4.936 ± 0.042

3.539 ± 0.026

8.819 ± 0.049

2.087 ± 0.021

5.696 ± 0.025

2.913 ± 0.03

9.884 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.022

2.554 ± 0.03

5.251 ± 0.034

2.994 ± 0.022

7.278 ± 0.046

5.499 ± 0.052

5.341 ± 0.053

7.007 ± 0.029

1.333 ± 0.02

2.208 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski