Amino acid dipepetide frequency for Stx2-converting phage 86

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.71AlaAla: 7.71 ± 0.635
1.212AlaCys: 1.212 ± 0.313
4.846AlaAsp: 4.846 ± 0.685
7.434AlaGlu: 7.434 ± 0.691
3.524AlaPhe: 3.524 ± 0.493
7.71AlaGly: 7.71 ± 1.183
1.707AlaHis: 1.707 ± 0.301
4.516AlaIle: 4.516 ± 0.518
4.461AlaLys: 4.461 ± 0.368
7.049AlaLeu: 7.049 ± 0.581
3.304AlaMet: 3.304 ± 0.54
2.698AlaAsn: 2.698 ± 0.389
3.249AlaPro: 3.249 ± 0.323
5.176AlaGln: 5.176 ± 0.661
6.168AlaArg: 6.168 ± 0.614
5.397AlaSer: 5.397 ± 0.571
4.791AlaThr: 4.791 ± 0.538
6.388AlaVal: 6.388 ± 0.669
1.542AlaTrp: 1.542 ± 0.224
2.313AlaTyr: 2.313 ± 0.318
0.0AlaXaa: 0.0 ± 0.0
Cys
1.322CysAla: 1.322 ± 0.304
0.33CysCys: 0.33 ± 0.141
0.496CysAsp: 0.496 ± 0.165
0.661CysGlu: 0.661 ± 0.244
0.441CysPhe: 0.441 ± 0.151
0.881CysGly: 0.881 ± 0.296
0.385CysHis: 0.385 ± 0.155
0.551CysIle: 0.551 ± 0.225
0.661CysLys: 0.661 ± 0.229
0.936CysLeu: 0.936 ± 0.236
0.11CysMet: 0.11 ± 0.076
0.275CysAsn: 0.275 ± 0.134
0.275CysPro: 0.275 ± 0.123
0.33CysGln: 0.33 ± 0.138
1.322CysArg: 1.322 ± 0.403
1.212CysSer: 1.212 ± 0.356
0.496CysThr: 0.496 ± 0.172
0.881CysVal: 0.881 ± 0.25
0.22CysTrp: 0.22 ± 0.124
0.275CysTyr: 0.275 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
5.782AspAla: 5.782 ± 0.493
0.771AspCys: 0.771 ± 0.229
3.965AspAsp: 3.965 ± 0.419
4.075AspGlu: 4.075 ± 0.499
1.432AspPhe: 1.432 ± 0.284
4.406AspGly: 4.406 ± 0.518
1.212AspHis: 1.212 ± 0.273
3.304AspIle: 3.304 ± 0.392
3.414AspLys: 3.414 ± 0.492
4.075AspLeu: 4.075 ± 0.399
1.707AspMet: 1.707 ± 0.28
2.864AspAsn: 2.864 ± 0.399
2.423AspPro: 2.423 ± 0.376
1.432AspGln: 1.432 ± 0.365
3.249AspArg: 3.249 ± 0.379
2.809AspSer: 2.809 ± 0.448
2.809AspThr: 2.809 ± 0.47
4.35AspVal: 4.35 ± 0.46
1.156AspTrp: 1.156 ± 0.26
1.652AspTyr: 1.652 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
6.718GluAla: 6.718 ± 0.648
0.991GluCys: 0.991 ± 0.265
1.982GluAsp: 1.982 ± 0.276
4.075GluGlu: 4.075 ± 0.445
2.313GluPhe: 2.313 ± 0.387
3.745GluGly: 3.745 ± 0.524
1.377GluHis: 1.377 ± 0.268
3.91GluIle: 3.91 ± 0.428
4.461GluLys: 4.461 ± 0.649
5.507GluLeu: 5.507 ± 0.516
2.423GluMet: 2.423 ± 0.403
2.753GluAsn: 2.753 ± 0.477
1.982GluPro: 1.982 ± 0.383
4.35GluGln: 4.35 ± 0.499
6.113GluArg: 6.113 ± 0.621
3.8GluSer: 3.8 ± 0.362
3.635GluThr: 3.635 ± 0.495
4.075GluVal: 4.075 ± 0.529
1.101GluTrp: 1.101 ± 0.24
1.982GluTyr: 1.982 ± 0.326
0.0GluXaa: 0.0 ± 0.0
Phe
2.533PheAla: 2.533 ± 0.409
0.496PheCys: 0.496 ± 0.215
2.038PheAsp: 2.038 ± 0.291
1.762PheGlu: 1.762 ± 0.372
0.771PhePhe: 0.771 ± 0.23
2.148PheGly: 2.148 ± 0.28
0.661PheHis: 0.661 ± 0.157
1.652PheIle: 1.652 ± 0.247
1.652PheLys: 1.652 ± 0.292
1.817PheLeu: 1.817 ± 0.277
0.991PheMet: 0.991 ± 0.194
1.542PheAsn: 1.542 ± 0.27
1.377PhePro: 1.377 ± 0.321
0.716PheGln: 0.716 ± 0.195
1.982PheArg: 1.982 ± 0.326
2.974PheSer: 2.974 ± 0.437
2.588PheThr: 2.588 ± 0.302
2.368PheVal: 2.368 ± 0.381
0.661PheTrp: 0.661 ± 0.208
1.432PheTyr: 1.432 ± 0.263
0.0PheXaa: 0.0 ± 0.0
Gly
5.452GlyAla: 5.452 ± 0.843
0.936GlyCys: 0.936 ± 0.334
4.516GlyAsp: 4.516 ± 0.625
6.333GlyGlu: 6.333 ± 1.266
3.029GlyPhe: 3.029 ± 0.448
4.516GlyGly: 4.516 ± 0.754
1.046GlyHis: 1.046 ± 0.215
3.91GlyIle: 3.91 ± 0.55
5.287GlyLys: 5.287 ± 0.896
5.562GlyLeu: 5.562 ± 0.42
2.809GlyMet: 2.809 ± 0.338
2.698GlyAsn: 2.698 ± 0.329
4.35GlyPro: 4.35 ± 2.458
2.533GlyGln: 2.533 ± 0.388
4.075GlyArg: 4.075 ± 0.561
4.35GlySer: 4.35 ± 0.538
2.974GlyThr: 2.974 ± 0.327
5.452GlyVal: 5.452 ± 0.615
1.046GlyTrp: 1.046 ± 0.244
2.588GlyTyr: 2.588 ± 0.385
0.0GlyXaa: 0.0 ± 0.0
His
1.487HisAla: 1.487 ± 0.257
0.165HisCys: 0.165 ± 0.103
1.046HisAsp: 1.046 ± 0.272
0.936HisGlu: 0.936 ± 0.199
0.716HisPhe: 0.716 ± 0.202
1.817HisGly: 1.817 ± 0.33
0.496HisHis: 0.496 ± 0.163
0.826HisIle: 0.826 ± 0.214
1.267HisLys: 1.267 ± 0.275
1.652HisLeu: 1.652 ± 0.331
0.275HisMet: 0.275 ± 0.105
0.826HisAsn: 0.826 ± 0.229
0.771HisPro: 0.771 ± 0.202
0.991HisGln: 0.991 ± 0.282
0.936HisArg: 0.936 ± 0.21
1.046HisSer: 1.046 ± 0.251
0.826HisThr: 0.826 ± 0.215
0.881HisVal: 0.881 ± 0.227
0.385HisTrp: 0.385 ± 0.154
0.771HisTyr: 0.771 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
4.075IleAla: 4.075 ± 0.41
0.771IleCys: 0.771 ± 0.24
3.414IleAsp: 3.414 ± 0.387
3.194IleGlu: 3.194 ± 0.481
1.046IlePhe: 1.046 ± 0.225
2.643IleGly: 2.643 ± 0.472
0.936IleHis: 0.936 ± 0.26
2.093IleIle: 2.093 ± 0.325
3.304IleLys: 3.304 ± 0.527
3.359IleLeu: 3.359 ± 0.54
1.046IleMet: 1.046 ± 0.199
3.579IleAsn: 3.579 ± 0.362
2.203IlePro: 2.203 ± 0.375
2.258IleGln: 2.258 ± 0.348
4.13IleArg: 4.13 ± 0.392
4.571IleSer: 4.571 ± 0.613
3.745IleThr: 3.745 ± 0.575
2.423IleVal: 2.423 ± 0.367
0.441IleTrp: 0.441 ± 0.188
1.652IleTyr: 1.652 ± 0.343
0.0IleXaa: 0.0 ± 0.0
Lys
5.672LysAla: 5.672 ± 0.494
0.716LysCys: 0.716 ± 0.252
2.643LysAsp: 2.643 ± 0.39
3.69LysGlu: 3.69 ± 0.43
1.267LysPhe: 1.267 ± 0.26
5.452LysGly: 5.452 ± 0.865
0.826LysHis: 0.826 ± 0.19
3.69LysIle: 3.69 ± 0.402
4.571LysLys: 4.571 ± 0.609
5.121LysLeu: 5.121 ± 0.505
1.707LysMet: 1.707 ± 0.295
3.579LysAsn: 3.579 ± 0.423
3.084LysPro: 3.084 ± 0.426
3.084LysGln: 3.084 ± 0.496
2.753LysArg: 2.753 ± 0.423
3.249LysSer: 3.249 ± 0.38
3.139LysThr: 3.139 ± 0.466
3.029LysVal: 3.029 ± 0.434
0.551LysTrp: 0.551 ± 0.171
2.148LysTyr: 2.148 ± 0.372
0.0LysXaa: 0.0 ± 0.0
Leu
8.371LeuAla: 8.371 ± 0.686
0.991LeuCys: 0.991 ± 0.264
3.469LeuAsp: 3.469 ± 0.477
4.571LeuGlu: 4.571 ± 0.454
2.533LeuPhe: 2.533 ± 0.367
4.516LeuGly: 4.516 ± 0.511
1.101LeuHis: 1.101 ± 0.215
3.249LeuIle: 3.249 ± 0.474
5.121LeuLys: 5.121 ± 0.492
6.884LeuLeu: 6.884 ± 0.674
2.148LeuMet: 2.148 ± 0.367
4.13LeuAsn: 4.13 ± 0.536
3.965LeuPro: 3.965 ± 0.498
2.919LeuGln: 2.919 ± 0.601
4.956LeuArg: 4.956 ± 0.569
6.058LeuSer: 6.058 ± 0.664
5.011LeuThr: 5.011 ± 0.554
5.011LeuVal: 5.011 ± 0.367
0.661LeuTrp: 0.661 ± 0.23
2.643LeuTyr: 2.643 ± 0.443
0.0LeuXaa: 0.0 ± 0.0
Met
3.194MetAla: 3.194 ± 0.478
0.055MetCys: 0.055 ± 0.053
1.542MetAsp: 1.542 ± 0.315
1.377MetGlu: 1.377 ± 0.238
0.606MetPhe: 0.606 ± 0.15
1.322MetGly: 1.322 ± 0.309
0.606MetHis: 0.606 ± 0.162
0.991MetIle: 0.991 ± 0.215
2.368MetLys: 2.368 ± 0.391
1.982MetLeu: 1.982 ± 0.28
1.046MetMet: 1.046 ± 0.236
1.267MetAsn: 1.267 ± 0.292
1.432MetPro: 1.432 ± 0.262
1.707MetGln: 1.707 ± 0.261
1.542MetArg: 1.542 ± 0.248
1.927MetSer: 1.927 ± 0.311
2.698MetThr: 2.698 ± 0.318
1.487MetVal: 1.487 ± 0.294
0.33MetTrp: 0.33 ± 0.131
0.716MetTyr: 0.716 ± 0.207
0.0MetXaa: 0.0 ± 0.0
Asn
4.681AsnAla: 4.681 ± 0.693
0.716AsnCys: 0.716 ± 0.214
2.313AsnAsp: 2.313 ± 0.384
2.753AsnGlu: 2.753 ± 0.481
1.377AsnPhe: 1.377 ± 0.265
3.91AsnGly: 3.91 ± 0.384
1.156AsnHis: 1.156 ± 0.298
2.864AsnIle: 2.864 ± 0.435
2.258AsnLys: 2.258 ± 0.322
3.304AsnLeu: 3.304 ± 0.36
1.267AsnMet: 1.267 ± 0.242
2.368AsnAsn: 2.368 ± 0.438
1.927AsnPro: 1.927 ± 0.296
2.093AsnGln: 2.093 ± 0.338
2.919AsnArg: 2.919 ± 0.502
2.423AsnSer: 2.423 ± 0.419
2.533AsnThr: 2.533 ± 0.388
1.597AsnVal: 1.597 ± 0.315
0.661AsnTrp: 0.661 ± 0.149
1.597AsnTyr: 1.597 ± 0.288
0.0AsnXaa: 0.0 ± 0.0
Pro
4.295ProAla: 4.295 ± 1.008
0.33ProCys: 0.33 ± 0.141
4.406ProAsp: 4.406 ± 0.553
4.791ProGlu: 4.791 ± 0.784
1.377ProPhe: 1.377 ± 0.22
3.469ProGly: 3.469 ± 0.718
0.496ProHis: 0.496 ± 0.158
1.432ProIle: 1.432 ± 0.274
1.982ProLys: 1.982 ± 0.297
2.423ProLeu: 2.423 ± 0.308
0.606ProMet: 0.606 ± 0.157
1.267ProAsn: 1.267 ± 0.314
1.762ProPro: 1.762 ± 0.347
2.368ProGln: 2.368 ± 0.7
1.927ProArg: 1.927 ± 0.34
2.753ProSer: 2.753 ± 0.517
2.203ProThr: 2.203 ± 0.383
4.681ProVal: 4.681 ± 0.407
0.661ProTrp: 0.661 ± 0.193
1.762ProTyr: 1.762 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
4.571GlnAla: 4.571 ± 0.513
0.551GlnCys: 0.551 ± 0.174
2.533GlnAsp: 2.533 ± 0.385
3.029GlnGlu: 3.029 ± 0.386
1.542GlnPhe: 1.542 ± 0.299
3.579GlnGly: 3.579 ± 0.983
0.771GlnHis: 0.771 ± 0.167
2.478GlnIle: 2.478 ± 0.411
3.194GlnLys: 3.194 ± 0.499
3.359GlnLeu: 3.359 ± 0.416
1.156GlnMet: 1.156 ± 0.348
1.927GlnAsn: 1.927 ± 0.369
2.203GlnPro: 2.203 ± 0.388
4.02GlnGln: 4.02 ± 0.874
3.084GlnArg: 3.084 ± 0.585
2.368GlnSer: 2.368 ± 0.314
2.533GlnThr: 2.533 ± 0.419
2.423GlnVal: 2.423 ± 0.471
0.771GlnTrp: 0.771 ± 0.172
1.432GlnTyr: 1.432 ± 0.283
0.0GlnXaa: 0.0 ± 0.0
Arg
4.791ArgAla: 4.791 ± 0.4
0.22ArgCys: 0.22 ± 0.119
3.8ArgAsp: 3.8 ± 0.658
5.121ArgGlu: 5.121 ± 0.58
2.313ArgPhe: 2.313 ± 0.355
4.571ArgGly: 4.571 ± 0.865
1.652ArgHis: 1.652 ± 0.257
3.91ArgIle: 3.91 ± 0.509
3.635ArgLys: 3.635 ± 0.413
4.791ArgLeu: 4.791 ± 0.446
1.817ArgMet: 1.817 ± 0.304
3.194ArgAsn: 3.194 ± 0.38
2.368ArgPro: 2.368 ± 0.398
2.974ArgGln: 2.974 ± 0.364
5.121ArgArg: 5.121 ± 0.587
4.075ArgSer: 4.075 ± 0.478
3.304ArgThr: 3.304 ± 0.523
3.469ArgVal: 3.469 ± 0.558
1.212ArgTrp: 1.212 ± 0.234
2.148ArgTyr: 2.148 ± 0.395
0.0ArgXaa: 0.0 ± 0.0
Ser
5.452SerAla: 5.452 ± 0.514
0.881SerCys: 0.881 ± 0.268
3.8SerAsp: 3.8 ± 0.436
4.35SerGlu: 4.35 ± 0.424
2.148SerPhe: 2.148 ± 0.293
6.498SerGly: 6.498 ± 0.602
0.826SerHis: 0.826 ± 0.258
2.809SerIle: 2.809 ± 0.369
3.359SerLys: 3.359 ± 0.464
6.278SerLeu: 6.278 ± 0.635
1.707SerMet: 1.707 ± 0.317
2.478SerAsn: 2.478 ± 0.415
3.029SerPro: 3.029 ± 0.397
2.753SerGln: 2.753 ± 0.395
3.965SerArg: 3.965 ± 0.499
3.194SerSer: 3.194 ± 0.5
3.855SerThr: 3.855 ± 0.406
4.24SerVal: 4.24 ± 0.571
0.551SerTrp: 0.551 ± 0.172
1.597SerTyr: 1.597 ± 0.36
0.0SerXaa: 0.0 ± 0.0
Thr
5.121ThrAla: 5.121 ± 0.421
0.606ThrCys: 0.606 ± 0.207
3.635ThrAsp: 3.635 ± 0.409
3.635ThrGlu: 3.635 ± 0.388
1.762ThrPhe: 1.762 ± 0.331
5.507ThrGly: 5.507 ± 0.844
1.101ThrHis: 1.101 ± 0.204
3.249ThrIle: 3.249 ± 0.291
2.974ThrLys: 2.974 ± 0.505
5.066ThrLeu: 5.066 ± 0.4
0.991ThrMet: 0.991 ± 0.23
2.093ThrAsn: 2.093 ± 0.367
3.965ThrPro: 3.965 ± 0.365
2.038ThrGln: 2.038 ± 0.438
2.368ThrArg: 2.368 ± 0.324
3.91ThrSer: 3.91 ± 0.388
3.139ThrThr: 3.139 ± 0.437
3.579ThrVal: 3.579 ± 0.447
0.771ThrTrp: 0.771 ± 0.188
1.046ThrTyr: 1.046 ± 0.211
0.0ThrXaa: 0.0 ± 0.0
Val
6.388ValAla: 6.388 ± 0.588
0.771ValCys: 0.771 ± 0.242
4.02ValAsp: 4.02 ± 0.421
3.8ValGlu: 3.8 ± 0.373
2.093ValPhe: 2.093 ± 0.34
3.745ValGly: 3.745 ± 0.513
0.661ValHis: 0.661 ± 0.159
3.139ValIle: 3.139 ± 0.452
3.69ValLys: 3.69 ± 0.434
5.617ValLeu: 5.617 ± 0.614
1.432ValMet: 1.432 ± 0.273
3.194ValAsn: 3.194 ± 0.369
3.084ValPro: 3.084 ± 0.389
2.698ValGln: 2.698 ± 0.412
3.855ValArg: 3.855 ± 0.705
5.011ValSer: 5.011 ± 0.591
3.855ValThr: 3.855 ± 0.443
4.35ValVal: 4.35 ± 0.393
0.716ValTrp: 0.716 ± 0.189
1.872ValTyr: 1.872 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
0.551TrpAla: 0.551 ± 0.198
0.165TrpCys: 0.165 ± 0.094
0.606TrpAsp: 0.606 ± 0.201
0.441TrpGlu: 0.441 ± 0.135
0.551TrpPhe: 0.551 ± 0.135
0.716TrpGly: 0.716 ± 0.249
0.385TrpHis: 0.385 ± 0.132
0.771TrpIle: 0.771 ± 0.181
1.046TrpLys: 1.046 ± 0.221
1.542TrpLeu: 1.542 ± 0.436
0.661TrpMet: 0.661 ± 0.185
0.551TrpAsn: 0.551 ± 0.171
0.385TrpPro: 0.385 ± 0.172
1.377TrpGln: 1.377 ± 0.206
1.542TrpArg: 1.542 ± 0.303
0.661TrpSer: 0.661 ± 0.179
0.606TrpThr: 0.606 ± 0.189
1.377TrpVal: 1.377 ± 0.268
0.33TrpTrp: 0.33 ± 0.147
0.22TrpTyr: 0.22 ± 0.092
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.029TyrAla: 3.029 ± 0.383
0.385TyrCys: 0.385 ± 0.165
1.817TyrAsp: 1.817 ± 0.303
1.267TyrGlu: 1.267 ± 0.258
1.322TyrPhe: 1.322 ± 0.222
2.313TyrGly: 2.313 ± 0.407
0.716TyrHis: 0.716 ± 0.203
1.542TyrIle: 1.542 ± 0.274
1.267TyrLys: 1.267 ± 0.242
1.982TyrLeu: 1.982 ± 0.322
0.936TyrMet: 0.936 ± 0.216
1.432TyrAsn: 1.432 ± 0.257
1.156TyrPro: 1.156 ± 0.235
1.707TyrGln: 1.707 ± 0.326
2.478TyrArg: 2.478 ± 0.451
2.038TyrSer: 2.038 ± 0.316
1.817TyrThr: 1.817 ± 0.392
1.927TyrVal: 1.927 ± 0.352
0.716TyrTrp: 0.716 ± 0.189
1.101TyrTyr: 1.101 ± 0.248
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (18160 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski