Sewage-associated circular DNA virus-14

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075J175|A0A075J175_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-14 OX=1519390 PE=3 SV=1
MM1 pKa = 7.49EE2 pKa = 5.0VPPPAYY8 pKa = 10.0SPPPAAVSAEE18 pKa = 4.1VVPRR22 pKa = 11.84PFRR25 pKa = 11.84LAGKK29 pKa = 10.05AIFLTWPQNDD39 pKa = 3.32VSKK42 pKa = 10.66EE43 pKa = 3.71DD44 pKa = 3.61LMAKK48 pKa = 9.77IVALWEE54 pKa = 4.65AKK56 pKa = 10.51LSWAVVAEE64 pKa = 4.39EE65 pKa = 4.18SHH67 pKa = 7.01KK68 pKa = 11.02SGEE71 pKa = 4.23PHH73 pKa = 4.94VHH75 pKa = 7.05AIVQFAEE82 pKa = 4.2RR83 pKa = 11.84LDD85 pKa = 3.88LKK87 pKa = 10.69NANPVLDD94 pKa = 4.42ALTGKK99 pKa = 9.52HH100 pKa = 6.22GNYY103 pKa = 9.84QSVKK107 pKa = 9.21SAKK110 pKa = 9.23KK111 pKa = 9.2VLRR114 pKa = 11.84YY115 pKa = 9.18VCKK118 pKa = 9.43DD119 pKa = 3.09GQYY122 pKa = 8.29ITHH125 pKa = 6.79GEE127 pKa = 4.09VPDD130 pKa = 4.7FSEE133 pKa = 5.17KK134 pKa = 10.44PKK136 pKa = 10.32LQDD139 pKa = 2.75WAAKK143 pKa = 10.21LIIEE147 pKa = 4.2EE148 pKa = 4.1QKK150 pKa = 9.75TYY152 pKa = 10.82RR153 pKa = 11.84DD154 pKa = 3.65LVRR157 pKa = 11.84EE158 pKa = 3.98QPGFSMMQKK167 pKa = 9.99RR168 pKa = 11.84KK169 pKa = 10.15LEE171 pKa = 4.0EE172 pKa = 4.18FIGWNKK178 pKa = 9.05RR179 pKa = 11.84QKK181 pKa = 10.68LADD184 pKa = 3.68SLLPWRR190 pKa = 11.84VLTPKK195 pKa = 10.28PSASPVHH202 pKa = 5.21VALWTFLRR210 pKa = 11.84EE211 pKa = 3.99NVLVPRR217 pKa = 11.84TPRR220 pKa = 11.84QAQLWLCGLPGVGKK234 pKa = 8.44TRR236 pKa = 11.84FLAYY240 pKa = 10.08LRR242 pKa = 11.84ARR244 pKa = 11.84LRR246 pKa = 11.84VYY248 pKa = 11.12DD249 pKa = 3.88MPRR252 pKa = 11.84SEE254 pKa = 5.39DD255 pKa = 4.03FYY257 pKa = 11.69DD258 pKa = 4.1EE259 pKa = 5.17YY260 pKa = 11.45EE261 pKa = 4.63DD262 pKa = 4.01GCFDD266 pKa = 4.95LVVLDD271 pKa = 4.69EE272 pKa = 4.5YY273 pKa = 10.97KK274 pKa = 10.68AHH276 pKa = 7.45KK277 pKa = 9.94KK278 pKa = 9.65IQFLNAWADD287 pKa = 3.87GQPLPLRR294 pKa = 11.84QKK296 pKa = 10.86GSQLTKK302 pKa = 10.27TDD304 pKa = 3.96NLPLIIVSNYY314 pKa = 10.16SIEE317 pKa = 4.12EE318 pKa = 4.13VYY320 pKa = 10.66RR321 pKa = 11.84PGVGRR326 pKa = 11.84DD327 pKa = 3.3ALVDD331 pKa = 3.44RR332 pKa = 11.84FTQVFVDD339 pKa = 3.65TAFVIDD345 pKa = 4.41DD346 pKa = 4.26PEE348 pKa = 4.55HH349 pKa = 7.54VEE351 pKa = 3.9

Molecular weight: 40.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075J175|A0A075J175_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-14 OX=1519390 PE=3 SV=1
MM1 pKa = 7.38SMKK4 pKa = 10.56YY5 pKa = 9.96LGKK8 pKa = 10.4RR9 pKa = 11.84KK10 pKa = 7.95PTTWKK15 pKa = 9.77PRR17 pKa = 11.84SNPLTMMRR25 pKa = 11.84AKK27 pKa = 10.6ARR29 pKa = 11.84TSAFRR34 pKa = 11.84MTPRR38 pKa = 11.84APLSTRR44 pKa = 11.84GWRR47 pKa = 11.84PFISTNHH54 pKa = 5.24EE55 pKa = 4.23LKK57 pKa = 11.05VQDD60 pKa = 4.57LASATYY66 pKa = 9.84QVNTTGSITLLAVPITGADD85 pKa = 3.3YY86 pKa = 10.65NARR89 pKa = 11.84IGRR92 pKa = 11.84KK93 pKa = 7.46VTLKK97 pKa = 9.88SCFIRR102 pKa = 11.84GYY104 pKa = 11.01LRR106 pKa = 11.84IEE108 pKa = 4.23PGAATVGVAQAQQCRR123 pKa = 11.84FMLVLDD129 pKa = 4.53LQPNGAVFAITDD141 pKa = 4.51LLNTADD147 pKa = 4.18PTSQLNLNNRR157 pKa = 11.84DD158 pKa = 3.51RR159 pKa = 11.84FRR161 pKa = 11.84VLQDD165 pKa = 2.93QEE167 pKa = 3.85IAFDD171 pKa = 3.38HH172 pKa = 6.22FNYY175 pKa = 8.8VTTATQTSEE184 pKa = 3.86AFGRR188 pKa = 11.84TIQSFKK194 pKa = 10.52KK195 pKa = 10.21YY196 pKa = 10.17KK197 pKa = 10.23RR198 pKa = 11.84LNCEE202 pKa = 3.83MIFNAVNGGTIADD215 pKa = 3.76IASGALYY222 pKa = 8.82MVWIGSNAAGANTDD236 pKa = 3.42ANAVLSTRR244 pKa = 11.84VRR246 pKa = 11.84YY247 pKa = 9.79SDD249 pKa = 3.32SS250 pKa = 3.22

Molecular weight: 27.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

601

250

351

300.5

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.484 ± 1.046

0.998 ± 0.123

5.491 ± 0.665

4.825 ± 1.722

4.16 ± 0.146

5.158 ± 0.513

1.664 ± 0.527

4.16 ± 0.634

6.156 ± 1.071

9.318 ± 0.803

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.632

4.16 ± 1.366

6.156 ± 1.315

4.493 ± 0.056

6.822 ± 0.718

5.491 ± 0.554

6.156 ± 2.343

7.82 ± 1.354

1.997 ± 0.486

3.328 ± 0.078
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski