Amino acid dipepetide frequency for Lachnospiraceae bacterium AM48-27BH

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.036AlaAla: 8.036 ± 0.143	1.043AlaCys: 1.043 ± 0.03	4.653AlaAsp: 4.653 ± 0.071	5.633AlaGlu: 5.633 ± 0.09	2.95AlaPhe: 2.95 ± 0.058	6.261AlaGly: 6.261 ± 0.099	1.166AlaHis: 1.166 ± 0.031	4.814AlaIle: 4.814 ± 0.085	4.685AlaLys: 4.685 ± 0.079	6.8AlaLeu: 6.8 ± 0.086	2.558AlaMet: 2.558 ± 0.048	2.501AlaAsn: 2.501 ± 0.06	2.18AlaPro: 2.18 ± 0.052	2.371AlaGln: 2.371 ± 0.047	3.072AlaArg: 3.072 ± 0.063	4.075AlaSer: 4.075 ± 0.077	3.297AlaThr: 3.297 ± 0.073	6.28AlaVal: 6.28 ± 0.096	0.785AlaTrp: 0.785 ± 0.031	2.898AlaTyr: 2.898 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.955CysAla: 0.955 ± 0.033	0.308CysCys: 0.308 ± 0.018	0.842CysAsp: 0.842 ± 0.03	0.91CysGlu: 0.91 ± 0.031	0.648CysPhe: 0.648 ± 0.027	1.546CysGly: 1.546 ± 0.043	0.322CysHis: 0.322 ± 0.019	1.04CysIle: 1.04 ± 0.034	0.807CysLys: 0.807 ± 0.03	1.207CysLeu: 1.207 ± 0.035	0.451CysMet: 0.451 ± 0.02	0.549CysAsn: 0.549 ± 0.021	0.636CysPro: 0.636 ± 0.025	0.571CysGln: 0.571 ± 0.024	0.748CysArg: 0.748 ± 0.028	0.946CysSer: 0.946 ± 0.031	0.707CysThr: 0.707 ± 0.023	1.03CysVal: 1.03 ± 0.028	0.152CysTrp: 0.152 ± 0.012	0.609CysTyr: 0.609 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.037AspAla: 4.037 ± 0.073	0.874AspCys: 0.874 ± 0.031	2.956AspAsp: 2.956 ± 0.051	4.505AspGlu: 4.505 ± 0.059	2.592AspPhe: 2.592 ± 0.045	4.968AspGly: 4.968 ± 0.09	1.218AspHis: 1.218 ± 0.035	3.78AspIle: 3.78 ± 0.067	3.072AspLys: 3.072 ± 0.062	4.894AspLeu: 4.894 ± 0.065	1.775AspMet: 1.775 ± 0.043	1.927AspAsn: 1.927 ± 0.046	2.008AspPro: 2.008 ± 0.043	2.13AspGln: 2.13 ± 0.052	2.624AspArg: 2.624 ± 0.05	2.979AspSer: 2.979 ± 0.06	3.026AspThr: 3.026 ± 0.056	3.595AspVal: 3.595 ± 0.06	0.67AspTrp: 0.67 ± 0.026	2.729AspTyr: 2.729 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	5.706GluAla: 5.706 ± 0.078	0.782GluCys: 0.782 ± 0.029	4.27GluAsp: 4.27 ± 0.063	6.889GluGlu: 6.889 ± 0.117	2.509GluPhe: 2.509 ± 0.054	4.123GluGly: 4.123 ± 0.065	1.524GluHis: 1.524 ± 0.043	5.284GluIle: 5.284 ± 0.075	6.539GluLys: 6.539 ± 0.095	6.743GluLeu: 6.743 ± 0.096	2.414GluMet: 2.414 ± 0.043	3.893GluAsn: 3.893 ± 0.058	1.971GluPro: 1.971 ± 0.051	3.286GluGln: 3.286 ± 0.063	3.675GluArg: 3.675 ± 0.064	3.222GluSer: 3.222 ± 0.059	4.191GluThr: 4.191 ± 0.074	4.295GluVal: 4.295 ± 0.065	0.824GluTrp: 0.824 ± 0.029	3.054GluTyr: 3.054 ± 0.056	0.0GluXaa: 0.0 ± 0.0
Phe	2.911PheAla: 2.911 ± 0.054	0.78PheCys: 0.78 ± 0.027	2.448PheAsp: 2.448 ± 0.045	2.49PheGlu: 2.49 ± 0.055	1.838PhePhe: 1.838 ± 0.053	2.921PheGly: 2.921 ± 0.053	0.935PheHis: 0.935 ± 0.032	2.462PheIle: 2.462 ± 0.053	1.946PheLys: 1.946 ± 0.048	4.028PheLeu: 4.028 ± 0.076	1.203PheMet: 1.203 ± 0.035	1.426PheAsn: 1.426 ± 0.038	1.493PhePro: 1.493 ± 0.039	1.597PheGln: 1.597 ± 0.04	1.756PheArg: 1.756 ± 0.037	2.721PheSer: 2.721 ± 0.058	2.304PheThr: 2.304 ± 0.047	2.656PheVal: 2.656 ± 0.055	0.487PheTrp: 0.487 ± 0.024	1.747PheTyr: 1.747 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	5.098GlyAla: 5.098 ± 0.091	1.278GlyCys: 1.278 ± 0.04	3.481GlyAsp: 3.481 ± 0.064	4.696GlyGlu: 4.696 ± 0.063	2.994GlyPhe: 2.994 ± 0.051	4.883GlyGly: 4.883 ± 0.105	1.325GlyHis: 1.325 ± 0.037	5.892GlyIle: 5.892 ± 0.08	5.408GlyLys: 5.408 ± 0.08	5.724GlyLeu: 5.724 ± 0.085	2.403GlyMet: 2.403 ± 0.051	3.139GlyAsn: 3.139 ± 0.076	1.311GlyPro: 1.311 ± 0.035	2.384GlyGln: 2.384 ± 0.049	3.228GlyArg: 3.228 ± 0.065	4.408GlySer: 4.408 ± 0.097	4.552GlyThr: 4.552 ± 0.104	4.862GlyVal: 4.862 ± 0.078	0.897GlyTrp: 0.897 ± 0.034	3.298GlyTyr: 3.298 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.16HisAla: 1.16 ± 0.035	0.335HisCys: 0.335 ± 0.02	0.985HisAsp: 0.985 ± 0.029	1.172HisGlu: 1.172 ± 0.036	0.958HisPhe: 0.958 ± 0.031	1.467HisGly: 1.467 ± 0.042	0.485HisHis: 0.485 ± 0.029	1.356HisIle: 1.356 ± 0.037	0.976HisLys: 0.976 ± 0.029	1.687HisLeu: 1.687 ± 0.04	0.607HisMet: 0.607 ± 0.024	0.734HisAsn: 0.734 ± 0.029	0.948HisPro: 0.948 ± 0.035	0.648HisGln: 0.648 ± 0.028	0.923HisArg: 0.923 ± 0.029	1.074HisSer: 1.074 ± 0.032	1.019HisThr: 1.019 ± 0.031	1.233HisVal: 1.233 ± 0.033	0.211HisTrp: 0.211 ± 0.013	0.805HisTyr: 0.805 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.217IleAla: 5.217 ± 0.085	1.316IleCys: 1.316 ± 0.04	3.764IleAsp: 3.764 ± 0.063	4.258IleGlu: 4.258 ± 0.07	2.77IlePhe: 2.77 ± 0.063	4.747IleGly: 4.747 ± 0.075	1.469IleHis: 1.469 ± 0.042	4.146IleIle: 4.146 ± 0.076	3.613IleLys: 3.613 ± 0.059	6.539IleLeu: 6.539 ± 0.094	1.811IleMet: 1.811 ± 0.044	2.638IleAsn: 2.638 ± 0.054	3.212IlePro: 3.212 ± 0.052	2.683IleGln: 2.683 ± 0.047	3.835IleArg: 3.835 ± 0.071	4.674IleSer: 4.674 ± 0.071	3.985IleThr: 3.985 ± 0.078	4.32IleVal: 4.32 ± 0.07	0.772IleTrp: 0.772 ± 0.027	2.684IleTyr: 2.684 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	5.11LysAla: 5.11 ± 0.084	0.711LysCys: 0.711 ± 0.028	3.713LysAsp: 3.713 ± 0.058	6.268LysGlu: 6.268 ± 0.081	1.759LysPhe: 1.759 ± 0.039	4.07LysGly: 4.07 ± 0.065	1.107LysHis: 1.107 ± 0.03	4.292LysIle: 4.292 ± 0.068	5.766LysLys: 5.766 ± 0.09	5.361LysLeu: 5.361 ± 0.071	2.005LysMet: 2.005 ± 0.045	3.344LysAsn: 3.344 ± 0.063	2.159LysPro: 2.159 ± 0.048	2.68LysGln: 2.68 ± 0.057	3.289LysArg: 3.289 ± 0.062	3.16LysSer: 3.16 ± 0.06	3.879LysThr: 3.879 ± 0.061	3.985LysVal: 3.985 ± 0.07	0.681LysTrp: 0.681 ± 0.026	2.617LysTyr: 2.617 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	6.817LeuAla: 6.817 ± 0.088	1.568LeuCys: 1.568 ± 0.044	5.129LeuAsp: 5.129 ± 0.076	6.403LeuGlu: 6.403 ± 0.093	3.706LeuPhe: 3.706 ± 0.067	5.578LeuGly: 5.578 ± 0.078	1.577LeuHis: 1.577 ± 0.041	5.694LeuIle: 5.694 ± 0.085	6.179LeuLys: 6.179 ± 0.083	8.329LeuLeu: 8.329 ± 0.124	2.732LeuMet: 2.732 ± 0.053	3.672LeuAsn: 3.672 ± 0.061	3.657LeuPro: 3.657 ± 0.064	3.011LeuGln: 3.011 ± 0.05	3.743LeuArg: 3.743 ± 0.074	5.985LeuSer: 5.985 ± 0.083	5.277LeuThr: 5.277 ± 0.076	5.568LeuVal: 5.568 ± 0.081	0.871LeuTrp: 0.871 ± 0.03	3.562LeuTyr: 3.562 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.654MetAla: 2.654 ± 0.054	0.38MetCys: 0.38 ± 0.019	1.924MetAsp: 1.924 ± 0.049	2.685MetGlu: 2.685 ± 0.052	1.025MetPhe: 1.025 ± 0.029	2.097MetGly: 2.097 ± 0.046	0.418MetHis: 0.418 ± 0.019	2.245MetIle: 2.245 ± 0.056	2.657MetLys: 2.657 ± 0.049	2.575MetLeu: 2.575 ± 0.049	0.985MetMet: 0.985 ± 0.03	1.503MetAsn: 1.503 ± 0.039	1.077MetPro: 1.077 ± 0.036	1.044MetGln: 1.044 ± 0.026	1.309MetArg: 1.309 ± 0.032	1.738MetSer: 1.738 ± 0.042	1.854MetThr: 1.854 ± 0.047	2.047MetVal: 2.047 ± 0.045	0.245MetTrp: 0.245 ± 0.016	0.902MetTyr: 0.902 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.065AsnAla: 3.065 ± 0.06	0.575AsnCys: 0.575 ± 0.027	2.019AsnAsp: 2.019 ± 0.048	2.556AsnGlu: 2.556 ± 0.06	1.527AsnPhe: 1.527 ± 0.038	3.513AsnGly: 3.513 ± 0.074	0.897AsnHis: 0.897 ± 0.029	2.788AsnIle: 2.788 ± 0.059	2.099AsnLys: 2.099 ± 0.051	3.709AsnLeu: 3.709 ± 0.065	1.305AsnMet: 1.305 ± 0.035	1.594AsnAsn: 1.594 ± 0.041	2.047AsnPro: 2.047 ± 0.045	1.665AsnGln: 1.665 ± 0.037	2.244AsnArg: 2.244 ± 0.046	2.315AsnSer: 2.315 ± 0.046	2.315AsnThr: 2.315 ± 0.058	2.607AsnVal: 2.607 ± 0.063	0.499AsnTrp: 0.499 ± 0.02	1.844AsnTyr: 1.844 ± 0.05	0.0AsnXaa: 0.0 ± 0.0
Pro	2.611ProAla: 2.611 ± 0.057	0.463ProCys: 0.463 ± 0.022	2.466ProAsp: 2.466 ± 0.047	3.716ProGlu: 3.716 ± 0.07	1.582ProPhe: 1.582 ± 0.045	2.445ProGly: 2.445 ± 0.056	0.638ProHis: 0.638 ± 0.023	2.133ProIle: 2.133 ± 0.045	2.044ProLys: 2.044 ± 0.046	2.762ProLeu: 2.762 ± 0.052	0.951ProMet: 0.951 ± 0.035	1.19ProAsn: 1.19 ± 0.037	0.863ProPro: 0.863 ± 0.034	1.165ProGln: 1.165 ± 0.038	1.058ProArg: 1.058 ± 0.034	1.86ProSer: 1.86 ± 0.044	1.595ProThr: 1.595 ± 0.039	3.177ProVal: 3.177 ± 0.053	0.367ProTrp: 0.367 ± 0.021	1.585ProTyr: 1.585 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.687GlnAla: 2.687 ± 0.058	0.454GlnCys: 0.454 ± 0.019	1.742GlnAsp: 1.742 ± 0.036	2.997GlnGlu: 2.997 ± 0.054	1.329GlnPhe: 1.329 ± 0.041	2.122GlnGly: 2.122 ± 0.047	0.511GlnHis: 0.511 ± 0.022	2.974GlnIle: 2.974 ± 0.051	3.116GlnLys: 3.116 ± 0.059	3.153GlnLeu: 3.153 ± 0.059	1.388GlnMet: 1.388 ± 0.039	1.771GlnAsn: 1.771 ± 0.046	1.087GlnPro: 1.087 ± 0.031	1.365GlnGln: 1.365 ± 0.045	1.593GlnArg: 1.593 ± 0.038	1.83GlnSer: 1.83 ± 0.046	2.008GlnThr: 2.008 ± 0.045	2.497GlnVal: 2.497 ± 0.05	0.437GlnTrp: 0.437 ± 0.017	1.638GlnTyr: 1.638 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.738ArgAla: 2.738 ± 0.055	0.683ArgCys: 0.683 ± 0.026	2.427ArgAsp: 2.427 ± 0.05	3.786ArgGlu: 3.786 ± 0.074	1.95ArgPhe: 1.95 ± 0.045	2.477ArgGly: 2.477 ± 0.052	0.873ArgHis: 0.873 ± 0.027	3.512ArgIle: 3.512 ± 0.064	3.679ArgLys: 3.679 ± 0.071	4.118ArgLeu: 4.118 ± 0.069	1.701ArgMet: 1.701 ± 0.038	2.097ArgAsn: 2.097 ± 0.049	1.462ArgPro: 1.462 ± 0.045	2.156ArgGln: 2.156 ± 0.049	2.494ArgArg: 2.494 ± 0.051	2.437ArgSer: 2.437 ± 0.05	2.511ArgThr: 2.511 ± 0.055	2.685ArgVal: 2.685 ± 0.05	0.515ArgTrp: 0.515 ± 0.021	2.111ArgTyr: 2.111 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	4.247SerAla: 4.247 ± 0.091	0.793SerCys: 0.793 ± 0.027	3.31SerAsp: 3.31 ± 0.054	3.737SerGlu: 3.737 ± 0.066	2.503SerPhe: 2.503 ± 0.05	5.131SerGly: 5.131 ± 0.093	1.064SerHis: 1.064 ± 0.032	3.928SerIle: 3.928 ± 0.076	3.113SerLys: 3.113 ± 0.06	5.181SerLeu: 5.181 ± 0.069	1.928SerMet: 1.928 ± 0.044	2.179SerAsn: 2.179 ± 0.053	1.852SerPro: 1.852 ± 0.045	2.101SerGln: 2.101 ± 0.052	2.782SerArg: 2.782 ± 0.052	3.701SerSer: 3.701 ± 0.095	2.794SerThr: 2.794 ± 0.06	4.05SerVal: 4.05 ± 0.062	0.686SerTrp: 0.686 ± 0.028	2.582SerTyr: 2.582 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.85ThrAla: 4.85 ± 0.106	0.637ThrCys: 0.637 ± 0.022	3.284ThrAsp: 3.284 ± 0.063	4.011ThrGlu: 4.011 ± 0.067	2.143ThrPhe: 2.143 ± 0.046	4.778ThrGly: 4.778 ± 0.084	0.921ThrHis: 0.921 ± 0.029	3.926ThrIle: 3.926 ± 0.077	2.817ThrLys: 2.817 ± 0.06	4.714ThrLeu: 4.714 ± 0.076	1.587ThrMet: 1.587 ± 0.04	1.997ThrAsn: 1.997 ± 0.052	2.305ThrPro: 2.305 ± 0.047	1.513ThrGln: 1.513 ± 0.041	2.338ThrArg: 2.338 ± 0.045	3.092ThrSer: 3.092 ± 0.064	3.084ThrThr: 3.084 ± 0.081	4.546ThrVal: 4.546 ± 0.094	0.667ThrTrp: 0.667 ± 0.027	2.159ThrTyr: 2.159 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	4.676ValAla: 4.676 ± 0.08	1.131ValCys: 1.131 ± 0.034	3.705ValAsp: 3.705 ± 0.062	4.579ValGlu: 4.579 ± 0.072	2.954ValPhe: 2.954 ± 0.06	4.159ValGly: 4.159 ± 0.073	1.171ValHis: 1.171 ± 0.033	4.936ValIle: 4.936 ± 0.072	4.361ValLys: 4.361 ± 0.069	6.457ValLeu: 6.457 ± 0.091	2.058ValMet: 2.058 ± 0.042	2.706ValAsn: 2.706 ± 0.061	2.591ValPro: 2.591 ± 0.046	2.059ValGln: 2.059 ± 0.045	3.005ValArg: 3.005 ± 0.058	4.474ValSer: 4.474 ± 0.077	4.219ValThr: 4.219 ± 0.095	4.701ValVal: 4.701 ± 0.083	0.749ValTrp: 0.749 ± 0.028	2.646ValTyr: 2.646 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.609TrpAla: 0.609 ± 0.025	0.165TrpCys: 0.165 ± 0.012	0.642TrpAsp: 0.642 ± 0.024	0.743TrpGlu: 0.743 ± 0.023	0.523TrpPhe: 0.523 ± 0.022	0.714TrpGly: 0.714 ± 0.028	0.218TrpHis: 0.218 ± 0.014	0.795TrpIle: 0.795 ± 0.03	1.0TrpLys: 1.0 ± 0.031	1.008TrpLeu: 1.008 ± 0.032	0.42TrpMet: 0.42 ± 0.019	0.67TrpAsn: 0.67 ± 0.026	0.247TrpPro: 0.247 ± 0.018	0.468TrpGln: 0.468 ± 0.024	0.481TrpArg: 0.481 ± 0.023	0.602TrpSer: 0.602 ± 0.025	0.504TrpThr: 0.504 ± 0.022	0.608TrpVal: 0.608 ± 0.024	0.171TrpTrp: 0.171 ± 0.014	0.533TrpTyr: 0.533 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.874TyrAla: 2.874 ± 0.045	0.71TyrCys: 0.71 ± 0.026	2.599TyrAsp: 2.599 ± 0.049	3.159TyrGlu: 3.159 ± 0.054	1.888TyrPhe: 1.888 ± 0.045	3.261TyrGly: 3.261 ± 0.054	0.947TyrHis: 0.947 ± 0.028	2.452TyrIle: 2.452 ± 0.049	2.079TyrLys: 2.079 ± 0.039	4.071TyrLeu: 4.071 ± 0.064	1.082TyrMet: 1.082 ± 0.031	1.651TyrAsn: 1.651 ± 0.041	1.566TyrPro: 1.566 ± 0.045	1.804TyrGln: 1.804 ± 0.042	2.217TyrArg: 2.217 ± 0.049	2.372TyrSer: 2.372 ± 0.044	2.245TyrThr: 2.245 ± 0.052	2.595TyrVal: 2.595 ± 0.052	0.433TyrTrp: 0.433 ± 0.021	1.927TyrTyr: 1.927 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3679 proteins (1102997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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