Streptococcus satellite phage Javan595

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZS96|A0A4D5ZS96_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan595 OX=2558765 GN=JavanS595_0012 PE=4 SV=1
MM1 pKa = 7.39LVTYY5 pKa = 8.27PALFYY10 pKa = 11.35YY11 pKa = 10.56DD12 pKa = 3.96DD13 pKa = 4.12TDD15 pKa = 3.96GASAPYY21 pKa = 9.78FVTFPDD27 pKa = 4.61FEE29 pKa = 5.3HH30 pKa = 7.07SATQGEE36 pKa = 4.54DD37 pKa = 3.02MANAMAMASDD47 pKa = 3.31WLGIHH52 pKa = 6.56LADD55 pKa = 4.36YY56 pKa = 10.61IEE58 pKa = 4.46NGRR61 pKa = 11.84DD62 pKa = 3.14IPTPTPINTLSLADD76 pKa = 4.14NNPFHH81 pKa = 7.66DD82 pKa = 5.34DD83 pKa = 3.28EE84 pKa = 7.48DD85 pKa = 4.59IEE87 pKa = 5.7LIYY90 pKa = 10.9DD91 pKa = 3.72PSKK94 pKa = 11.33SFISMVMVDD103 pKa = 3.25VAEE106 pKa = 4.14YY107 pKa = 10.54LGSQEE112 pKa = 4.64PIKK115 pKa = 10.26KK116 pKa = 8.15TLTIPRR122 pKa = 11.84WADD125 pKa = 3.13TLGRR129 pKa = 11.84EE130 pKa = 4.01LGLNFSQTLTDD141 pKa = 5.45AIADD145 pKa = 4.03KK146 pKa = 10.56KK147 pKa = 10.31IHH149 pKa = 5.92AA150 pKa = 5.0

Molecular weight: 16.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZRF1|A0A4D5ZRF1_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan595 OX=2558765 GN=JavanS595_0019 PE=4 SV=1
MM1 pKa = 7.77LSIAKK6 pKa = 9.94QNIKK10 pKa = 10.44HH11 pKa = 6.42RR12 pKa = 11.84GLGIKK17 pKa = 10.06KK18 pKa = 10.29LIEE21 pKa = 4.28KK22 pKa = 10.25LSNSYY27 pKa = 10.42KK28 pKa = 10.26QGNKK32 pKa = 9.76KK33 pKa = 10.13GSKK36 pKa = 9.27LAWYY40 pKa = 9.27LLKK43 pKa = 10.93SQQ45 pKa = 4.33

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

2415

45

282

127.1

14.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.969 ± 0.646

0.331 ± 0.134

6.17 ± 0.58

8.199 ± 0.84

3.685 ± 0.338

5.052 ± 0.338

1.615 ± 0.244

8.199 ± 0.623

9.689 ± 0.695

9.275 ± 0.654

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.307

6.046 ± 0.534

3.313 ± 0.589

4.306 ± 0.485

4.058 ± 0.338

5.88 ± 0.526

6.542 ± 0.447

4.969 ± 0.645

1.118 ± 0.239

3.892 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski