Amino acid dipepetide frequency for Mycobacterium phage KyMonks1A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.602AlaAla: 12.602 ± 1.442
0.553AlaCys: 0.553 ± 0.201
6.947AlaAsp: 6.947 ± 0.71
6.824AlaGlu: 6.824 ± 0.929
3.135AlaPhe: 3.135 ± 0.551
7.561AlaGly: 7.561 ± 0.765
1.598AlaHis: 1.598 ± 0.339
4.365AlaIle: 4.365 ± 0.5
3.873AlaLys: 3.873 ± 0.721
8.914AlaLeu: 8.914 ± 0.885
2.52AlaMet: 2.52 ± 0.347
2.336AlaAsn: 2.336 ± 0.352
5.102AlaPro: 5.102 ± 0.704
3.197AlaGln: 3.197 ± 0.491
6.147AlaArg: 6.147 ± 0.741
4.734AlaSer: 4.734 ± 0.535
5.902AlaThr: 5.902 ± 0.502
9.16AlaVal: 9.16 ± 0.887
1.721AlaTrp: 1.721 ± 0.384
2.643AlaTyr: 2.643 ± 0.369
0.0AlaXaa: 0.0 ± 0.0
Cys
0.676CysAla: 0.676 ± 0.209
0.0CysCys: 0.0 ± 0.0
0.43CysAsp: 0.43 ± 0.142
0.553CysGlu: 0.553 ± 0.177
0.123CysPhe: 0.123 ± 0.093
0.553CysGly: 0.553 ± 0.184
0.123CysHis: 0.123 ± 0.084
0.307CysIle: 0.307 ± 0.157
0.369CysLys: 0.369 ± 0.178
0.553CysLeu: 0.553 ± 0.219
0.123CysMet: 0.123 ± 0.078
0.184CysAsn: 0.184 ± 0.1
0.307CysPro: 0.307 ± 0.137
0.184CysGln: 0.184 ± 0.112
0.369CysArg: 0.369 ± 0.144
0.307CysSer: 0.307 ± 0.124
0.307CysThr: 0.307 ± 0.142
0.307CysVal: 0.307 ± 0.145
0.246CysTrp: 0.246 ± 0.107
0.061CysTyr: 0.061 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
6.147AspAla: 6.147 ± 0.638
0.553AspCys: 0.553 ± 0.176
4.918AspAsp: 4.918 ± 0.506
3.75AspGlu: 3.75 ± 0.53
2.52AspPhe: 2.52 ± 0.32
5.779AspGly: 5.779 ± 0.626
1.107AspHis: 1.107 ± 0.289
2.643AspIle: 2.643 ± 0.448
2.766AspLys: 2.766 ± 0.493
6.947AspLeu: 6.947 ± 0.592
1.229AspMet: 1.229 ± 0.258
1.475AspAsn: 1.475 ± 0.308
5.041AspPro: 5.041 ± 0.584
1.598AspGln: 1.598 ± 0.327
3.873AspArg: 3.873 ± 0.468
3.258AspSer: 3.258 ± 0.505
3.627AspThr: 3.627 ± 0.401
4.918AspVal: 4.918 ± 0.577
1.967AspTrp: 1.967 ± 0.341
2.09AspTyr: 2.09 ± 0.375
0.0AspXaa: 0.0 ± 0.0
Glu
6.024GluAla: 6.024 ± 0.786
0.246GluCys: 0.246 ± 0.149
5.102GluAsp: 5.102 ± 0.585
5.041GluGlu: 5.041 ± 0.596
2.213GluPhe: 2.213 ± 0.382
4.119GluGly: 4.119 ± 0.509
1.107GluHis: 1.107 ± 0.29
3.688GluIle: 3.688 ± 0.431
2.705GluLys: 2.705 ± 0.426
6.701GluLeu: 6.701 ± 0.589
1.475GluMet: 1.475 ± 0.314
1.598GluAsn: 1.598 ± 0.376
2.582GluPro: 2.582 ± 0.408
3.012GluGln: 3.012 ± 0.399
3.934GluArg: 3.934 ± 0.659
3.504GluSer: 3.504 ± 0.412
3.996GluThr: 3.996 ± 0.535
5.656GluVal: 5.656 ± 0.618
1.537GluTrp: 1.537 ± 0.33
2.275GluTyr: 2.275 ± 0.49
0.0GluXaa: 0.0 ± 0.0
Phe
2.582PheAla: 2.582 ± 0.453
0.307PheCys: 0.307 ± 0.177
2.459PheAsp: 2.459 ± 0.346
2.213PheGlu: 2.213 ± 0.307
0.676PhePhe: 0.676 ± 0.213
3.381PheGly: 3.381 ± 0.465
0.615PheHis: 0.615 ± 0.262
1.168PheIle: 1.168 ± 0.299
1.352PheLys: 1.352 ± 0.273
2.52PheLeu: 2.52 ± 0.483
0.553PheMet: 0.553 ± 0.198
1.414PheAsn: 1.414 ± 0.354
1.598PhePro: 1.598 ± 0.299
0.984PheGln: 0.984 ± 0.22
2.09PheArg: 2.09 ± 0.371
1.598PheSer: 1.598 ± 0.271
2.336PheThr: 2.336 ± 0.436
1.967PheVal: 1.967 ± 0.391
0.615PheTrp: 0.615 ± 0.175
0.984PheTyr: 0.984 ± 0.232
0.0PheXaa: 0.0 ± 0.0
Gly
7.377GlyAla: 7.377 ± 1.04
0.615GlyCys: 0.615 ± 0.194
5.594GlyAsp: 5.594 ± 0.589
4.488GlyGlu: 4.488 ± 0.544
2.766GlyPhe: 2.766 ± 0.457
8.238GlyGly: 8.238 ± 1.513
1.721GlyHis: 1.721 ± 0.339
4.488GlyIle: 4.488 ± 0.795
3.135GlyLys: 3.135 ± 0.517
7.807GlyLeu: 7.807 ± 0.839
1.66GlyMet: 1.66 ± 0.359
3.135GlyAsn: 3.135 ± 0.411
4.426GlyPro: 4.426 ± 0.592
2.213GlyGln: 2.213 ± 0.416
4.795GlyArg: 4.795 ± 0.555
5.902GlySer: 5.902 ± 0.645
4.672GlyThr: 4.672 ± 0.643
5.41GlyVal: 5.41 ± 0.572
2.52GlyTrp: 2.52 ± 0.356
2.766GlyTyr: 2.766 ± 0.393
0.0GlyXaa: 0.0 ± 0.0
His
1.721HisAla: 1.721 ± 0.339
0.061HisCys: 0.061 ± 0.061
1.229HisAsp: 1.229 ± 0.242
1.721HisGlu: 1.721 ± 0.312
0.799HisPhe: 0.799 ± 0.266
1.475HisGly: 1.475 ± 0.324
0.738HisHis: 0.738 ± 0.19
0.861HisIle: 0.861 ± 0.203
1.045HisLys: 1.045 ± 0.298
1.291HisLeu: 1.291 ± 0.338
0.184HisMet: 0.184 ± 0.108
0.307HisAsn: 0.307 ± 0.154
1.352HisPro: 1.352 ± 0.268
0.799HisGln: 0.799 ± 0.184
1.107HisArg: 1.107 ± 0.26
0.676HisSer: 0.676 ± 0.204
1.045HisThr: 1.045 ± 0.279
1.414HisVal: 1.414 ± 0.282
0.553HisTrp: 0.553 ± 0.18
0.615HisTyr: 0.615 ± 0.203
0.0HisXaa: 0.0 ± 0.0
Ile
6.209IleAla: 6.209 ± 0.753
0.246IleCys: 0.246 ± 0.118
3.197IleAsp: 3.197 ± 0.402
3.75IleGlu: 3.75 ± 0.499
0.861IlePhe: 0.861 ± 0.276
3.934IleGly: 3.934 ± 0.532
0.861IleHis: 0.861 ± 0.207
2.029IleIle: 2.029 ± 0.33
2.029IleLys: 2.029 ± 0.373
3.688IleLeu: 3.688 ± 0.56
0.738IleMet: 0.738 ± 0.217
1.967IleAsn: 1.967 ± 0.371
2.889IlePro: 2.889 ± 0.395
1.537IleGln: 1.537 ± 0.36
3.75IleArg: 3.75 ± 0.554
3.074IleSer: 3.074 ± 0.581
3.627IleThr: 3.627 ± 0.489
2.705IleVal: 2.705 ± 0.56
0.553IleTrp: 0.553 ± 0.167
1.598IleTyr: 1.598 ± 0.286
0.0IleXaa: 0.0 ± 0.0
Lys
3.32LysAla: 3.32 ± 0.618
0.307LysCys: 0.307 ± 0.15
2.52LysAsp: 2.52 ± 0.47
1.967LysGlu: 1.967 ± 0.359
1.414LysPhe: 1.414 ± 0.316
2.275LysGly: 2.275 ± 0.391
1.229LysHis: 1.229 ± 0.282
2.582LysIle: 2.582 ± 0.493
1.844LysLys: 1.844 ± 0.43
3.443LysLeu: 3.443 ± 0.458
1.045LysMet: 1.045 ± 0.221
1.475LysAsn: 1.475 ± 0.291
2.275LysPro: 2.275 ± 0.428
1.66LysGln: 1.66 ± 0.436
2.889LysArg: 2.889 ± 0.466
2.828LysSer: 2.828 ± 0.372
2.582LysThr: 2.582 ± 0.466
3.258LysVal: 3.258 ± 0.528
0.799LysTrp: 0.799 ± 0.252
0.799LysTyr: 0.799 ± 0.22
0.0LysXaa: 0.0 ± 0.0
Leu
9.528LeuAla: 9.528 ± 0.889
0.43LeuCys: 0.43 ± 0.147
5.902LeuAsp: 5.902 ± 0.561
5.287LeuGlu: 5.287 ± 0.596
2.029LeuPhe: 2.029 ± 0.338
7.561LeuGly: 7.561 ± 0.879
1.414LeuHis: 1.414 ± 0.318
4.795LeuIle: 4.795 ± 0.471
4.119LeuLys: 4.119 ± 0.459
6.27LeuLeu: 6.27 ± 0.604
1.721LeuMet: 1.721 ± 0.312
3.074LeuAsn: 3.074 ± 0.385
5.717LeuPro: 5.717 ± 0.579
2.766LeuGln: 2.766 ± 0.548
5.963LeuArg: 5.963 ± 0.601
6.578LeuSer: 6.578 ± 0.534
6.455LeuThr: 6.455 ± 0.468
4.795LeuVal: 4.795 ± 0.668
1.352LeuTrp: 1.352 ± 0.355
2.336LeuTyr: 2.336 ± 0.463
0.0LeuXaa: 0.0 ± 0.0
Met
2.582MetAla: 2.582 ± 0.303
0.0MetCys: 0.0 ± 0.0
1.168MetAsp: 1.168 ± 0.306
1.66MetGlu: 1.66 ± 0.375
0.553MetPhe: 0.553 ± 0.174
1.291MetGly: 1.291 ± 0.277
0.246MetHis: 0.246 ± 0.111
0.861MetIle: 0.861 ± 0.261
0.922MetLys: 0.922 ± 0.251
1.291MetLeu: 1.291 ± 0.288
0.123MetMet: 0.123 ± 0.096
1.107MetAsn: 1.107 ± 0.203
0.984MetPro: 0.984 ± 0.264
0.492MetGln: 0.492 ± 0.163
0.984MetArg: 0.984 ± 0.23
2.152MetSer: 2.152 ± 0.44
2.336MetThr: 2.336 ± 0.341
1.045MetVal: 1.045 ± 0.291
0.246MetTrp: 0.246 ± 0.106
0.369MetTyr: 0.369 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
3.197AsnAla: 3.197 ± 0.496
0.184AsnCys: 0.184 ± 0.103
2.152AsnAsp: 2.152 ± 0.364
1.967AsnGlu: 1.967 ± 0.384
0.922AsnPhe: 0.922 ± 0.287
3.688AsnGly: 3.688 ± 0.51
0.799AsnHis: 0.799 ± 0.213
1.844AsnIle: 1.844 ± 0.345
0.553AsnLys: 0.553 ± 0.253
2.09AsnLeu: 2.09 ± 0.345
0.615AsnMet: 0.615 ± 0.16
0.861AsnAsn: 0.861 ± 0.269
2.397AsnPro: 2.397 ± 0.387
1.107AsnGln: 1.107 ± 0.241
1.229AsnArg: 1.229 ± 0.27
1.598AsnSer: 1.598 ± 0.428
2.09AsnThr: 2.09 ± 0.347
2.52AsnVal: 2.52 ± 0.414
0.861AsnTrp: 0.861 ± 0.215
1.107AsnTyr: 1.107 ± 0.317
0.0AsnXaa: 0.0 ± 0.0
Pro
5.041ProAla: 5.041 ± 0.515
0.43ProCys: 0.43 ± 0.141
4.119ProAsp: 4.119 ± 0.516
3.996ProGlu: 3.996 ± 0.471
2.397ProPhe: 2.397 ± 0.403
5.287ProGly: 5.287 ± 0.631
0.861ProHis: 0.861 ± 0.267
2.459ProIle: 2.459 ± 0.428
2.029ProLys: 2.029 ± 0.293
4.672ProLeu: 4.672 ± 0.538
0.984ProMet: 0.984 ± 0.315
1.414ProAsn: 1.414 ± 0.305
2.705ProPro: 2.705 ± 0.412
1.537ProGln: 1.537 ± 0.334
2.828ProArg: 2.828 ± 0.489
3.75ProSer: 3.75 ± 0.515
3.75ProThr: 3.75 ± 0.587
4.119ProVal: 4.119 ± 0.469
0.861ProTrp: 0.861 ± 0.268
1.66ProTyr: 1.66 ± 0.371
0.0ProXaa: 0.0 ± 0.0
Gln
3.012GlnAla: 3.012 ± 0.512
0.061GlnCys: 0.061 ± 0.059
1.66GlnAsp: 1.66 ± 0.373
1.783GlnGlu: 1.783 ± 0.315
1.352GlnPhe: 1.352 ± 0.255
2.397GlnGly: 2.397 ± 0.301
0.615GlnHis: 0.615 ± 0.156
2.889GlnIle: 2.889 ± 0.549
1.229GlnLys: 1.229 ± 0.322
3.934GlnLeu: 3.934 ± 0.469
0.738GlnMet: 0.738 ± 0.223
0.615GlnAsn: 0.615 ± 0.208
1.844GlnPro: 1.844 ± 0.355
1.783GlnGln: 1.783 ± 0.344
1.844GlnArg: 1.844 ± 0.35
1.844GlnSer: 1.844 ± 0.363
1.783GlnThr: 1.783 ± 0.338
2.643GlnVal: 2.643 ± 0.39
0.553GlnTrp: 0.553 ± 0.153
0.553GlnTyr: 0.553 ± 0.163
0.0GlnXaa: 0.0 ± 0.0
Arg
5.717ArgAla: 5.717 ± 0.721
0.676ArgCys: 0.676 ± 0.224
3.135ArgAsp: 3.135 ± 0.385
4.488ArgGlu: 4.488 ± 0.654
1.783ArgPhe: 1.783 ± 0.354
4.734ArgGly: 4.734 ± 0.744
1.107ArgHis: 1.107 ± 0.248
2.951ArgIle: 2.951 ± 0.388
3.197ArgLys: 3.197 ± 0.471
6.209ArgLeu: 6.209 ± 0.791
2.09ArgMet: 2.09 ± 0.399
2.397ArgAsn: 2.397 ± 0.417
2.397ArgPro: 2.397 ± 0.449
2.029ArgGln: 2.029 ± 0.301
5.102ArgArg: 5.102 ± 0.653
3.688ArgSer: 3.688 ± 0.478
2.705ArgThr: 2.705 ± 0.508
5.287ArgVal: 5.287 ± 0.571
1.229ArgTrp: 1.229 ± 0.28
1.598ArgTyr: 1.598 ± 0.293
0.0ArgXaa: 0.0 ± 0.0
Ser
6.455SerAla: 6.455 ± 0.723
0.492SerCys: 0.492 ± 0.201
3.873SerAsp: 3.873 ± 0.52
4.488SerGlu: 4.488 ± 0.396
1.783SerPhe: 1.783 ± 0.441
5.779SerGly: 5.779 ± 0.703
1.352SerHis: 1.352 ± 0.264
2.52SerIle: 2.52 ± 0.437
2.275SerLys: 2.275 ± 0.325
5.471SerLeu: 5.471 ± 0.543
1.475SerMet: 1.475 ± 0.275
2.213SerAsn: 2.213 ± 0.447
2.951SerPro: 2.951 ± 0.48
2.152SerGln: 2.152 ± 0.288
2.766SerArg: 2.766 ± 0.388
3.566SerSer: 3.566 ± 0.644
3.258SerThr: 3.258 ± 0.449
4.119SerVal: 4.119 ± 0.541
1.352SerTrp: 1.352 ± 0.281
1.414SerTyr: 1.414 ± 0.349
0.0SerXaa: 0.0 ± 0.0
Thr
6.147ThrAla: 6.147 ± 0.878
0.184ThrCys: 0.184 ± 0.12
4.057ThrAsp: 4.057 ± 0.525
4.057ThrGlu: 4.057 ± 0.508
2.336ThrPhe: 2.336 ± 0.375
6.762ThrGly: 6.762 ± 0.562
1.168ThrHis: 1.168 ± 0.267
2.52ThrIle: 2.52 ± 0.551
2.705ThrLys: 2.705 ± 0.338
5.84ThrLeu: 5.84 ± 0.723
0.861ThrMet: 0.861 ± 0.198
1.783ThrAsn: 1.783 ± 0.368
3.996ThrPro: 3.996 ± 0.522
1.906ThrGln: 1.906 ± 0.307
3.811ThrArg: 3.811 ± 0.612
3.75ThrSer: 3.75 ± 0.589
4.119ThrThr: 4.119 ± 0.484
5.348ThrVal: 5.348 ± 0.66
1.045ThrTrp: 1.045 ± 0.287
1.844ThrTyr: 1.844 ± 0.311
0.0ThrXaa: 0.0 ± 0.0
Val
7.131ValAla: 7.131 ± 0.759
0.307ValCys: 0.307 ± 0.134
4.979ValAsp: 4.979 ± 0.559
5.164ValGlu: 5.164 ± 0.56
2.275ValPhe: 2.275 ± 0.359
5.102ValGly: 5.102 ± 0.713
1.414ValHis: 1.414 ± 0.25
3.75ValIle: 3.75 ± 0.597
3.012ValLys: 3.012 ± 0.42
5.902ValLeu: 5.902 ± 0.521
1.168ValMet: 1.168 ± 0.359
2.705ValAsn: 2.705 ± 0.399
4.365ValPro: 4.365 ± 0.561
2.152ValGln: 2.152 ± 0.398
5.164ValArg: 5.164 ± 0.615
4.365ValSer: 4.365 ± 0.402
6.27ValThr: 6.27 ± 0.739
5.225ValVal: 5.225 ± 0.682
1.475ValTrp: 1.475 ± 0.311
2.275ValTyr: 2.275 ± 0.4
0.0ValXaa: 0.0 ± 0.0
Trp
1.66TrpAla: 1.66 ± 0.338
0.184TrpCys: 0.184 ± 0.109
1.475TrpAsp: 1.475 ± 0.323
1.045TrpGlu: 1.045 ± 0.265
0.984TrpPhe: 0.984 ± 0.242
1.598TrpGly: 1.598 ± 0.259
0.369TrpHis: 0.369 ± 0.144
0.984TrpIle: 0.984 ± 0.215
0.307TrpLys: 0.307 ± 0.177
2.09TrpLeu: 2.09 ± 0.332
0.369TrpMet: 0.369 ± 0.188
0.553TrpAsn: 0.553 ± 0.208
0.738TrpPro: 0.738 ± 0.215
0.799TrpGln: 0.799 ± 0.239
1.352TrpArg: 1.352 ± 0.345
1.045TrpSer: 1.045 ± 0.228
1.721TrpThr: 1.721 ± 0.422
2.275TrpVal: 2.275 ± 0.322
0.615TrpTrp: 0.615 ± 0.232
0.43TrpTyr: 0.43 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.705TyrAla: 2.705 ± 0.437
0.246TyrCys: 0.246 ± 0.133
1.291TyrAsp: 1.291 ± 0.311
2.275TyrGlu: 2.275 ± 0.369
0.492TyrPhe: 0.492 ± 0.16
2.275TyrGly: 2.275 ± 0.395
0.553TyrHis: 0.553 ± 0.191
1.537TyrIle: 1.537 ± 0.322
1.045TyrLys: 1.045 ± 0.205
2.459TyrLeu: 2.459 ± 0.425
0.676TyrMet: 0.676 ± 0.177
1.168TyrAsn: 1.168 ± 0.357
1.229TyrPro: 1.229 ± 0.267
1.229TyrGln: 1.229 ± 0.32
2.52TyrArg: 2.52 ± 0.461
1.537TyrSer: 1.537 ± 0.34
1.66TyrThr: 1.66 ± 0.398
2.029TyrVal: 2.029 ± 0.338
0.492TyrTrp: 0.492 ± 0.172
0.43TyrTyr: 0.43 ± 0.141
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (16268 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski