Helianthus annuus (Common sunflower)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae; Asteroideae; Heliantheae alliance; Heliantheae; Helianthus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A251TI58|A0A251TI58_HELAN Fatty acyl-CoA reductase OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr10g0291761 PE=3 SV=1
MM1 pKa = 7.62DD2 pKa = 4.15TSDD5 pKa = 4.22TGDD8 pKa = 3.3SDD10 pKa = 3.94TTGPLPMVSDD20 pKa = 5.27DD21 pKa = 3.61IVSSEE26 pKa = 4.59HH27 pKa = 6.11EE28 pKa = 4.49VHH30 pKa = 6.09TSDD33 pKa = 4.6YY34 pKa = 10.96TSTDD38 pKa = 3.38DD39 pKa = 6.61DD40 pKa = 4.43DD41 pKa = 4.69FQPFALPDD49 pKa = 3.92GADD52 pKa = 3.58EE53 pKa = 5.43PIVGDD58 pKa = 3.76PAEE61 pKa = 4.78DD62 pKa = 3.69LPLVVIPAPIPLASYY77 pKa = 8.76PAYY80 pKa = 10.12EE81 pKa = 4.38LMPGAEE87 pKa = 4.38ADD89 pKa = 3.49GDD91 pKa = 3.51IDD93 pKa = 4.81LFEE96 pKa = 5.54DD97 pKa = 4.15EE98 pKa = 4.77PLEE101 pKa = 5.43DD102 pKa = 4.29EE103 pKa = 4.87VPDD106 pKa = 4.43PALLPAGGLLMIAGAPVGDD125 pKa = 4.49SPVHH129 pKa = 6.02SPVPDD134 pKa = 3.35SCEE137 pKa = 4.14SVASAPSHH145 pKa = 5.38EE146 pKa = 4.47VSIQHH151 pKa = 6.07FVHH154 pKa = 7.63DD155 pKa = 4.98SDD157 pKa = 4.74PDD159 pKa = 3.67QASSAAPIPSLVFEE173 pKa = 4.97HH174 pKa = 7.42DD175 pKa = 4.07VIEE178 pKa = 5.08DD179 pKa = 3.7SDD181 pKa = 4.25PVFPPGFDD189 pKa = 3.49PDD191 pKa = 3.91RR192 pKa = 11.84DD193 pKa = 3.48IEE195 pKa = 4.54YY196 pKa = 10.37IPMDD200 pKa = 3.59HH201 pKa = 7.42HH202 pKa = 6.67MEE204 pKa = 4.64DD205 pKa = 3.71PVDD208 pKa = 3.92PVDD211 pKa = 5.27PIDD214 pKa = 5.58PIDD217 pKa = 4.22PDD219 pKa = 4.25FDD221 pKa = 4.85FDD223 pKa = 4.14MAFDD227 pKa = 4.31DD228 pKa = 4.94PEE230 pKa = 4.22PAVAPEE236 pKa = 3.55QAAAFDD242 pKa = 4.42PVHH245 pKa = 6.1EE246 pKa = 5.36HH247 pKa = 7.17DD248 pKa = 5.21LVHH251 pKa = 7.17ADD253 pKa = 3.63VPVDD257 pKa = 3.75PVLADD262 pKa = 3.88PPVGDD267 pKa = 4.84LPVDD271 pKa = 4.06DD272 pKa = 5.29VPLLADD278 pKa = 3.89DD279 pKa = 4.71HH280 pKa = 7.29VVDD283 pKa = 5.1PFVDD287 pKa = 4.05PPLIADD293 pKa = 3.73VPVDD297 pKa = 3.15HH298 pKa = 7.13HH299 pKa = 7.22VDD301 pKa = 3.21HH302 pKa = 7.23VDD304 pKa = 3.41PVIAPFDD311 pKa = 3.84LVPIEE316 pKa = 4.54PEE318 pKa = 3.48HH319 pKa = 7.75ALFAEE324 pKa = 4.81HH325 pKa = 7.04MDD327 pKa = 4.15PPDD330 pKa = 3.72EE331 pKa = 4.27EE332 pKa = 5.14AQHH335 pKa = 5.62GWIPADD341 pKa = 3.65EE342 pKa = 5.0DD343 pKa = 4.16VPPLPPHH350 pKa = 5.9HH351 pKa = 7.0TEE353 pKa = 3.48AHH355 pKa = 5.63HH356 pKa = 6.69TDD358 pKa = 3.86FSFQIPPFVPPAGPGEE374 pKa = 4.43GSSAHH379 pKa = 6.41PFGHH383 pKa = 6.65IPMPMPFMPQMTPVPSSVHH402 pKa = 5.12VAPFDD407 pKa = 3.78PTSTPLFWSSSSPMPPTDD425 pKa = 3.98PYY427 pKa = 11.15HH428 pKa = 6.48PFHH431 pKa = 6.83MGHH434 pKa = 6.67TIEE437 pKa = 4.87DD438 pKa = 3.55VLMSFVHH445 pKa = 5.27QHH447 pKa = 6.73DD448 pKa = 3.88SHH450 pKa = 6.0SQRR453 pKa = 11.84LQEE456 pKa = 4.53LEE458 pKa = 3.86RR459 pKa = 11.84AQLSFGPYY467 pKa = 10.1LGQTSSSFQPFRR479 pKa = 11.84SLPPDD484 pKa = 3.8FAARR488 pKa = 11.84LTTMEE493 pKa = 3.94QQIASVICTQQAMEE507 pKa = 4.69EE508 pKa = 4.25DD509 pKa = 3.87WLHH512 pKa = 6.42LRR514 pKa = 11.84RR515 pKa = 11.84LLYY518 pKa = 9.72THH520 pKa = 7.28FPPPPPPSAA529 pKa = 3.84

Molecular weight:
57.52 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A251SQ45|A0A251SQ45_HELAN Putative UDP-glucuronosyl/UDP-glucosyltransferase OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr13g0393321 PE=3 SV=1
MM1 pKa = 7.16QLRR4 pKa = 11.84GKK6 pKa = 9.97LVLVGRR12 pKa = 11.84RR13 pKa = 11.84VPGARR18 pKa = 11.84VLATLKK24 pKa = 10.45LARR27 pKa = 11.84LRR29 pKa = 11.84LSQRR33 pKa = 11.84MKK35 pKa = 8.71RR36 pKa = 11.84WKK38 pKa = 10.32RR39 pKa = 11.84KK40 pKa = 8.54MPLPNLLGGRR50 pKa = 11.84GVGARR55 pKa = 11.84LQLLSLPRR63 pKa = 11.84LHH65 pKa = 6.93LWW67 pKa = 3.33

Molecular weight:
7.7 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51240

0

51240

17848194

50

5399

348.3

39.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.115 ± 0.011

1.919 ± 0.006

5.358 ± 0.009

6.076 ± 0.011

4.397 ± 0.008

6.385 ± 0.011

2.513 ± 0.006

5.579 ± 0.01

6.258 ± 0.011

9.664 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.005

4.716 ± 0.008

4.785 ± 0.012

3.546 ± 0.007

5.115 ± 0.008

8.513 ± 0.013

5.319 ± 0.007

6.797 ± 0.01

1.338 ± 0.005

3.032 ± 0.006

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski