Nique virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Nique phlebovirus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2W3S7|F2W3S7_9VIRU Nonstructural protein OS=Nique virus OX=629739 PE=4 SV=1
MM1 pKa = 7.64SNMMRR6 pKa = 11.84YY7 pKa = 9.97AFDD10 pKa = 4.08LPHH13 pKa = 6.27ITRR16 pKa = 11.84NATDD20 pKa = 3.42YY21 pKa = 10.7NRR23 pKa = 11.84VYY25 pKa = 11.07VEE27 pKa = 3.98YY28 pKa = 10.81SAFNNYY34 pKa = 8.81HH35 pKa = 5.98SAPVCRR41 pKa = 11.84YY42 pKa = 9.17KK43 pKa = 10.9GAEE46 pKa = 4.21FPCTSFKK53 pKa = 10.88VSSKK57 pKa = 9.28VRR59 pKa = 11.84YY60 pKa = 9.24RR61 pKa = 11.84LSTFIMRR68 pKa = 11.84DD69 pKa = 3.68EE70 pKa = 5.27LPLSWGQAHH79 pKa = 5.27QQVKK83 pKa = 9.67GASPSFFDD91 pKa = 3.29TTMEE95 pKa = 4.49KK96 pKa = 9.73ISYY99 pKa = 10.24LNLDD103 pKa = 3.93RR104 pKa = 11.84QLKK107 pKa = 8.73WSEE110 pKa = 3.85PNLRR114 pKa = 11.84EE115 pKa = 4.12ALSWPLGKK123 pKa = 8.61PTFTFFKK130 pKa = 10.37LSQIHH135 pKa = 6.02SYY137 pKa = 9.81EE138 pKa = 3.97YY139 pKa = 10.24HH140 pKa = 5.99WEE142 pKa = 4.18EE143 pKa = 3.79KK144 pKa = 10.42CAFATLVMRR153 pKa = 11.84CGKK156 pKa = 10.45GEE158 pKa = 4.07QIDD161 pKa = 4.0DD162 pKa = 4.14SLVNLYY168 pKa = 10.74KK169 pKa = 10.91SIDD172 pKa = 3.47VEE174 pKa = 3.97LAEE177 pKa = 5.23RR178 pKa = 11.84EE179 pKa = 4.16IFSGNFTGQNIEE191 pKa = 4.0KK192 pKa = 9.86EE193 pKa = 3.87IAYY196 pKa = 9.59VQLLRR201 pKa = 11.84MMTALPYY208 pKa = 10.08DD209 pKa = 3.58YY210 pKa = 10.54HH211 pKa = 8.12EE212 pKa = 5.13SEE214 pKa = 4.32FHH216 pKa = 7.14SSLFQYY222 pKa = 11.29VMDD225 pKa = 4.94LYY227 pKa = 11.26KK228 pKa = 10.83VISPSLLGNKK238 pKa = 8.35KK239 pKa = 9.13WNPQSTWDD247 pKa = 4.07DD248 pKa = 3.52SLRR251 pKa = 11.84DD252 pKa = 3.59LTYY255 pKa = 10.09DD256 pKa = 3.26TDD258 pKa = 4.14SDD260 pKa = 4.22FEE262 pKa = 4.69SGGCCSCC269 pKa = 5.01

Molecular weight: 31.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2W3S7|F2W3S7_9VIRU Nonstructural protein OS=Nique virus OX=629739 PE=4 SV=1
MM1 pKa = 7.82SFEE4 pKa = 4.59DD5 pKa = 4.8LAIEE9 pKa = 4.14LAGHH13 pKa = 5.95EE14 pKa = 4.33VDD16 pKa = 3.99EE17 pKa = 4.22QTIASWVQAFAYY29 pKa = 10.12QGFDD33 pKa = 2.84ARR35 pKa = 11.84RR36 pKa = 11.84VIEE39 pKa = 3.99LLKK42 pKa = 11.06SRR44 pKa = 11.84GGDD47 pKa = 2.66AWMEE51 pKa = 4.13DD52 pKa = 3.72AKK54 pKa = 11.46QMIILCLTRR63 pKa = 11.84GNKK66 pKa = 6.14PTKK69 pKa = 10.12MMTKK73 pKa = 9.85MSDD76 pKa = 2.88KK77 pKa = 10.58GKK79 pKa = 10.65KK80 pKa = 8.79IVQGLIKK87 pKa = 10.29KK88 pKa = 10.16YY89 pKa = 9.19NLKK92 pKa = 10.27EE93 pKa = 4.19GNPSRR98 pKa = 11.84DD99 pKa = 3.68DD100 pKa = 3.49LTLSRR105 pKa = 11.84ITAALAGYY113 pKa = 6.71TCQATEE119 pKa = 4.09VVEE122 pKa = 4.27EE123 pKa = 4.0FLPVTGRR130 pKa = 11.84HH131 pKa = 5.12MDD133 pKa = 3.81GLSKK137 pKa = 10.75NYY139 pKa = 8.63PRR141 pKa = 11.84AMMHH145 pKa = 6.7PSFAGLIDD153 pKa = 4.28PGLPAEE159 pKa = 4.34VLATITDD166 pKa = 3.81AFSLFMVQFSRR177 pKa = 11.84TINPRR182 pKa = 11.84NRR184 pKa = 11.84GLSTSEE190 pKa = 3.77VLSTFDD196 pKa = 3.76RR197 pKa = 11.84PMNAAINSSFLTRR210 pKa = 11.84DD211 pKa = 3.01QRR213 pKa = 11.84KK214 pKa = 9.28AFLKK218 pKa = 10.8NLGILDD224 pKa = 4.33GNLQPSEE231 pKa = 4.39SVKK234 pKa = 10.64AAAKK238 pKa = 10.3AFRR241 pKa = 11.84TAKK244 pKa = 10.47

Molecular weight: 27.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3936

244

2088

984.0

111.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.268 ± 0.897

2.795 ± 0.7

6.047 ± 0.858

6.174 ± 0.111

4.649 ± 0.498

6.072 ± 0.604

2.261 ± 0.127

6.657 ± 0.733

7.088 ± 0.319

9.578 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.176 ± 0.244

4.294 ± 0.394

3.532 ± 0.217

3.303 ± 0.324

4.649 ± 0.231

9.782 ± 0.349

5.412 ± 0.673

5.666 ± 0.36

1.296 ± 0.161

3.303 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski