Amino acid dipepetide frequency for Streptomycetaceae bacterium MP113-05

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.778AlaAla: 19.778 ± 0.182	1.137AlaCys: 1.137 ± 0.03	8.437AlaAsp: 8.437 ± 0.079	8.91AlaGlu: 8.91 ± 0.09	3.543AlaPhe: 3.543 ± 0.05	13.128AlaGly: 13.128 ± 0.11	3.01AlaHis: 3.01 ± 0.053	2.808AlaIle: 2.808 ± 0.05	2.491AlaLys: 2.491 ± 0.051	14.244AlaLeu: 14.244 ± 0.114	2.565AlaMet: 2.565 ± 0.046	1.72AlaAsn: 1.72 ± 0.038	6.709AlaPro: 6.709 ± 0.075	3.608AlaGln: 3.608 ± 0.05	10.376AlaArg: 10.376 ± 0.099	5.575AlaSer: 5.575 ± 0.06	6.718AlaThr: 6.718 ± 0.072	12.197AlaVal: 12.197 ± 0.105	1.76AlaTrp: 1.76 ± 0.03	2.652AlaTyr: 2.652 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	1.123CysAla: 1.123 ± 0.028	0.097CysCys: 0.097 ± 0.009	0.53CysAsp: 0.53 ± 0.02	0.417CysGlu: 0.417 ± 0.016	0.236CysPhe: 0.236 ± 0.013	1.057CysGly: 1.057 ± 0.026	0.208CysHis: 0.208 ± 0.011	0.186CysIle: 0.186 ± 0.011	0.117CysLys: 0.117 ± 0.008	0.775CysLeu: 0.775 ± 0.023	0.133CysMet: 0.133 ± 0.01	0.13CysAsn: 0.13 ± 0.01	0.506CysPro: 0.506 ± 0.021	0.153CysGln: 0.153 ± 0.011	0.7CysArg: 0.7 ± 0.022	0.476CysSer: 0.476 ± 0.02	0.592CysThr: 0.592 ± 0.02	0.695CysVal: 0.695 ± 0.023	0.131CysTrp: 0.131 ± 0.009	0.152CysTyr: 0.152 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.007AspAla: 8.007 ± 0.08	0.457AspCys: 0.457 ± 0.02	3.887AspAsp: 3.887 ± 0.051	4.163AspGlu: 4.163 ± 0.054	1.705AspPhe: 1.705 ± 0.034	6.985AspGly: 6.985 ± 0.079	1.5AspHis: 1.5 ± 0.031	1.668AspIle: 1.668 ± 0.034	0.976AspLys: 0.976 ± 0.03	6.279AspLeu: 6.279 ± 0.075	0.868AspMet: 0.868 ± 0.021	0.79AspAsn: 0.79 ± 0.024	4.507AspPro: 4.507 ± 0.061	1.404AspGln: 1.404 ± 0.033	5.257AspArg: 5.257 ± 0.071	2.55AspSer: 2.55 ± 0.043	3.198AspThr: 3.198 ± 0.05	5.162AspVal: 5.162 ± 0.06	0.967AspTrp: 0.967 ± 0.025	1.125AspTyr: 1.125 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	7.596GluAla: 7.596 ± 0.086	0.413GluCys: 0.413 ± 0.017	3.274GluAsp: 3.274 ± 0.056	3.94GluGlu: 3.94 ± 0.06	1.429GluPhe: 1.429 ± 0.032	4.807GluGly: 4.807 ± 0.056	1.662GluHis: 1.662 ± 0.035	2.202GluIle: 2.202 ± 0.039	1.577GluLys: 1.577 ± 0.036	6.912GluLeu: 6.912 ± 0.073	0.97GluMet: 0.97 ± 0.026	1.095GluAsn: 1.095 ± 0.025	3.587GluPro: 3.587 ± 0.049	2.4GluGln: 2.4 ± 0.047	5.856GluArg: 5.856 ± 0.063	2.786GluSer: 2.786 ± 0.042	3.201GluThr: 3.201 ± 0.045	4.93GluVal: 4.93 ± 0.068	0.804GluTrp: 0.804 ± 0.024	1.137GluTyr: 1.137 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.746PheAla: 3.746 ± 0.046	0.292PheCys: 0.292 ± 0.014	2.02PheAsp: 2.02 ± 0.038	1.481PheGlu: 1.481 ± 0.035	0.9PhePhe: 0.9 ± 0.027	2.998PheGly: 2.998 ± 0.048	0.661PheHis: 0.661 ± 0.021	0.633PheIle: 0.633 ± 0.022	0.434PheLys: 0.434 ± 0.017	2.649PheLeu: 2.649 ± 0.044	0.381PheMet: 0.381 ± 0.015	0.48PheAsn: 0.48 ± 0.02	1.365PhePro: 1.365 ± 0.03	0.651PheGln: 0.651 ± 0.019	1.921PheArg: 1.921 ± 0.035	1.47PheSer: 1.47 ± 0.03	1.979PheThr: 1.979 ± 0.041	2.264PheVal: 2.264 ± 0.038	0.389PheTrp: 0.389 ± 0.015	0.53PheTyr: 0.53 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	11.133GlyAla: 11.133 ± 0.094	0.888GlyCys: 0.888 ± 0.023	5.607GlyAsp: 5.607 ± 0.057	5.858GlyGlu: 5.858 ± 0.063	2.866GlyPhe: 2.866 ± 0.047	9.409GlyGly: 9.409 ± 0.095	2.473GlyHis: 2.473 ± 0.045	3.144GlyIle: 3.144 ± 0.051	2.184GlyLys: 2.184 ± 0.047	9.561GlyLeu: 9.561 ± 0.091	2.203GlyMet: 2.203 ± 0.039	1.671GlyAsn: 1.671 ± 0.035	5.345GlyPro: 5.345 ± 0.066	2.53GlyGln: 2.53 ± 0.044	8.267GlyArg: 8.267 ± 0.086	5.528GlySer: 5.528 ± 0.063	6.35GlyThr: 6.35 ± 0.069	7.91GlyVal: 7.91 ± 0.085	1.662GlyTrp: 1.662 ± 0.037	2.164GlyTyr: 2.164 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.855HisAla: 2.855 ± 0.044	0.232HisCys: 0.232 ± 0.014	1.525HisAsp: 1.525 ± 0.029	1.304HisGlu: 1.304 ± 0.028	0.674HisPhe: 0.674 ± 0.023	2.654HisGly: 2.654 ± 0.043	0.784HisHis: 0.784 ± 0.025	0.664HisIle: 0.664 ± 0.024	0.337HisLys: 0.337 ± 0.017	2.665HisLeu: 2.665 ± 0.046	0.33HisMet: 0.33 ± 0.012	0.378HisAsn: 0.378 ± 0.016	1.844HisPro: 1.844 ± 0.041	0.678HisGln: 0.678 ± 0.022	2.43HisArg: 2.43 ± 0.042	1.091HisSer: 1.091 ± 0.027	1.433HisThr: 1.433 ± 0.037	1.871HisVal: 1.871 ± 0.036	0.362HisTrp: 0.362 ± 0.014	0.504HisTyr: 0.504 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	4.303IleAla: 4.303 ± 0.06	0.256IleCys: 0.256 ± 0.014	2.114IleAsp: 2.114 ± 0.038	1.88IleGlu: 1.88 ± 0.038	0.644IlePhe: 0.644 ± 0.025	3.152IleGly: 3.152 ± 0.05	0.574IleHis: 0.574 ± 0.018	0.743IleIle: 0.743 ± 0.024	0.619IleLys: 0.619 ± 0.024	2.188IleLeu: 2.188 ± 0.04	0.411IleMet: 0.411 ± 0.017	0.606IleAsn: 0.606 ± 0.019	1.568IlePro: 1.568 ± 0.034	0.614IleGln: 0.614 ± 0.02	2.144IleArg: 2.144 ± 0.038	1.499IleSer: 1.499 ± 0.033	1.883IleThr: 1.883 ± 0.035	2.485IleVal: 2.485 ± 0.04	0.311IleTrp: 0.311 ± 0.014	0.441IleTyr: 0.441 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	2.472LysAla: 2.472 ± 0.052	0.129LysCys: 0.129 ± 0.01	1.158LysAsp: 1.158 ± 0.034	1.183LysGlu: 1.183 ± 0.031	0.393LysPhe: 0.393 ± 0.017	1.65LysGly: 1.65 ± 0.04	0.432LysHis: 0.432 ± 0.016	0.792LysIle: 0.792 ± 0.028	0.802LysLys: 0.802 ± 0.032	1.867LysLeu: 1.867 ± 0.039	0.344LysMet: 0.344 ± 0.015	0.489LysAsn: 0.489 ± 0.022	1.198LysPro: 1.198 ± 0.031	0.713LysGln: 0.713 ± 0.025	1.482LysArg: 1.482 ± 0.034	1.053LysSer: 1.053 ± 0.032	1.182LysThr: 1.182 ± 0.032	1.685LysVal: 1.685 ± 0.038	0.215LysTrp: 0.215 ± 0.012	0.395LysTyr: 0.395 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	14.756LeuAla: 14.756 ± 0.131	0.902LeuCys: 0.902 ± 0.025	6.754LeuAsp: 6.754 ± 0.064	5.219LeuGlu: 5.219 ± 0.058	2.635LeuPhe: 2.635 ± 0.048	9.236LeuGly: 9.236 ± 0.088	2.553LeuHis: 2.553 ± 0.049	3.03LeuIle: 3.03 ± 0.048	1.791LeuLys: 1.791 ± 0.038	11.527LeuLeu: 11.527 ± 0.112	1.643LeuMet: 1.643 ± 0.038	1.641LeuAsn: 1.641 ± 0.035	6.536LeuPro: 6.536 ± 0.08	2.392LeuGln: 2.392 ± 0.045	9.162LeuArg: 9.162 ± 0.093	5.414LeuSer: 5.414 ± 0.062	6.857LeuThr: 6.857 ± 0.073	8.855LeuVal: 8.855 ± 0.079	1.229LeuTrp: 1.229 ± 0.029	1.767LeuTyr: 1.767 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.247MetAla: 2.247 ± 0.042	0.134MetCys: 0.134 ± 0.01	0.894MetAsp: 0.894 ± 0.023	0.869MetGlu: 0.869 ± 0.023	0.475MetPhe: 0.475 ± 0.02	1.34MetGly: 1.34 ± 0.031	0.412MetHis: 0.412 ± 0.016	0.638MetIle: 0.638 ± 0.023	0.428MetLys: 0.428 ± 0.017	1.88MetLeu: 1.88 ± 0.036	0.312MetMet: 0.312 ± 0.015	0.418MetAsn: 0.418 ± 0.014	1.171MetPro: 1.171 ± 0.023	0.487MetGln: 0.487 ± 0.019	1.486MetArg: 1.486 ± 0.029	1.277MetSer: 1.277 ± 0.028	1.514MetThr: 1.514 ± 0.032	1.336MetVal: 1.336 ± 0.029	0.227MetTrp: 0.227 ± 0.011	0.32MetTyr: 0.32 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.073AsnAla: 2.073 ± 0.042	0.159AsnCys: 0.159 ± 0.011	0.946AsnAsp: 0.946 ± 0.025	0.853AsnGlu: 0.853 ± 0.025	0.453AsnPhe: 0.453 ± 0.02	1.794AsnGly: 1.794 ± 0.037	0.376AsnHis: 0.376 ± 0.016	0.578AsnIle: 0.578 ± 0.021	0.361AsnLys: 0.361 ± 0.017	1.616AsnLeu: 1.616 ± 0.036	0.286AsnMet: 0.286 ± 0.013	0.357AsnAsn: 0.357 ± 0.016	1.219AsnPro: 1.219 ± 0.027	0.459AsnGln: 0.459 ± 0.019	1.288AsnArg: 1.288 ± 0.028	0.843AsnSer: 0.843 ± 0.025	0.977AsnThr: 0.977 ± 0.03	1.373AsnVal: 1.373 ± 0.031	0.263AsnTrp: 0.263 ± 0.015	0.392AsnTyr: 0.392 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	8.107ProAla: 8.107 ± 0.085	0.377ProCys: 0.377 ± 0.014	4.553ProAsp: 4.553 ± 0.062	4.606ProGlu: 4.606 ± 0.06	1.525ProPhe: 1.525 ± 0.031	6.894ProGly: 6.894 ± 0.081	1.53ProHis: 1.53 ± 0.035	1.036ProIle: 1.036 ± 0.029	1.048ProLys: 1.048 ± 0.028	5.302ProLeu: 5.302 ± 0.06	0.998ProMet: 0.998 ± 0.027	0.822ProAsn: 0.822 ± 0.025	3.549ProPro: 3.549 ± 0.073	1.885ProGln: 1.885 ± 0.039	3.986ProArg: 3.986 ± 0.05	3.206ProSer: 3.206 ± 0.044	3.012ProThr: 3.012 ± 0.053	5.518ProVal: 5.518 ± 0.064	0.861ProTrp: 0.861 ± 0.029	1.324ProTyr: 1.324 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.6GlnAla: 3.6 ± 0.054	0.182GlnCys: 0.182 ± 0.013	1.417GlnAsp: 1.417 ± 0.03	1.552GlnGlu: 1.552 ± 0.032	0.627GlnPhe: 0.627 ± 0.018	2.22GlnGly: 2.22 ± 0.036	0.716GlnHis: 0.716 ± 0.023	1.036GlnIle: 1.036 ± 0.028	0.607GlnLys: 0.607 ± 0.025	2.888GlnLeu: 2.888 ± 0.051	0.51GlnMet: 0.51 ± 0.02	0.517GlnAsn: 0.517 ± 0.021	1.696GlnPro: 1.696 ± 0.036	1.281GlnGln: 1.281 ± 0.033	2.619GlnArg: 2.619 ± 0.045	1.244GlnSer: 1.244 ± 0.029	1.342GlnThr: 1.342 ± 0.027	2.488GlnVal: 2.488 ± 0.043	0.407GlnTrp: 0.407 ± 0.019	0.554GlnTyr: 0.554 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.822ArgAla: 9.822 ± 0.099	0.686ArgCys: 0.686 ± 0.023	4.691ArgAsp: 4.691 ± 0.057	5.286ArgGlu: 5.286 ± 0.064	2.432ArgPhe: 2.432 ± 0.042	6.225ArgGly: 6.225 ± 0.064	2.332ArgHis: 2.332 ± 0.048	3.094ArgIle: 3.094 ± 0.052	1.7ArgLys: 1.7 ± 0.042	9.135ArgLeu: 9.135 ± 0.079	1.915ArgMet: 1.915 ± 0.034	1.514ArgAsn: 1.514 ± 0.034	5.003ArgPro: 5.003 ± 0.065	2.376ArgGln: 2.376 ± 0.04	8.331ArgArg: 8.331 ± 0.09	4.388ArgSer: 4.388 ± 0.051	5.575ArgThr: 5.575 ± 0.064	6.29ArgVal: 6.29 ± 0.07	1.44ArgTrp: 1.44 ± 0.03	1.834ArgTyr: 1.834 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.593SerAla: 6.593 ± 0.068	0.409SerCys: 0.409 ± 0.017	2.741SerAsp: 2.741 ± 0.043	2.578SerGlu: 2.578 ± 0.039	1.544SerPhe: 1.544 ± 0.03	6.203SerGly: 6.203 ± 0.068	1.126SerHis: 1.126 ± 0.026	1.258SerIle: 1.258 ± 0.033	0.894SerLys: 0.894 ± 0.028	4.963SerLeu: 4.963 ± 0.06	1.012SerMet: 1.012 ± 0.026	0.806SerAsn: 0.806 ± 0.025	3.171SerPro: 3.171 ± 0.049	1.229SerGln: 1.229 ± 0.035	3.994SerArg: 3.994 ± 0.051	2.913SerSer: 2.913 ± 0.05	2.955SerThr: 2.955 ± 0.044	4.35SerVal: 4.35 ± 0.055	0.846SerTrp: 0.846 ± 0.023	1.128SerTyr: 1.128 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	8.456ThrAla: 8.456 ± 0.079	0.45ThrCys: 0.45 ± 0.016	3.643ThrAsp: 3.643 ± 0.052	3.448ThrGlu: 3.448 ± 0.04	1.609ThrPhe: 1.609 ± 0.035	6.879ThrGly: 6.879 ± 0.065	1.276ThrHis: 1.276 ± 0.034	1.42ThrIle: 1.42 ± 0.031	1.004ThrLys: 1.004 ± 0.027	5.742ThrLeu: 5.742 ± 0.065	1.003ThrMet: 1.003 ± 0.028	0.903ThrAsn: 0.903 ± 0.026	3.914ThrPro: 3.914 ± 0.054	1.251ThrGln: 1.251 ± 0.03	4.065ThrArg: 4.065 ± 0.059	3.152ThrSer: 3.152 ± 0.042	3.639ThrThr: 3.639 ± 0.058	6.141ThrVal: 6.141 ± 0.069	0.816ThrTrp: 0.816 ± 0.025	1.195ThrTyr: 1.195 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	10.647ValAla: 10.647 ± 0.11	0.877ValCys: 0.877 ± 0.022	5.06ValAsp: 5.06 ± 0.062	5.03ValGlu: 5.03 ± 0.052	2.412ValPhe: 2.412 ± 0.041	6.809ValGly: 6.809 ± 0.067	2.261ValHis: 2.261 ± 0.041	2.607ValIle: 2.607 ± 0.038	1.611ValLys: 1.611 ± 0.035	9.882ValLeu: 9.882 ± 0.097	1.466ValMet: 1.466 ± 0.032	1.615ValAsn: 1.615 ± 0.028	5.445ValPro: 5.445 ± 0.063	2.23ValGln: 2.23 ± 0.034	7.566ValArg: 7.566 ± 0.079	4.462ValSer: 4.462 ± 0.057	5.536ValThr: 5.536 ± 0.065	8.14ValVal: 8.14 ± 0.081	1.1ValTrp: 1.1 ± 0.027	1.539ValTyr: 1.539 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.505TrpAla: 1.505 ± 0.035	0.156TrpCys: 0.156 ± 0.009	0.81TrpAsp: 0.81 ± 0.025	0.788TrpGlu: 0.788 ± 0.021	0.512TrpPhe: 0.512 ± 0.018	1.048TrpGly: 1.048 ± 0.026	0.36TrpHis: 0.36 ± 0.016	0.473TrpIle: 0.473 ± 0.018	0.334TrpLys: 0.334 ± 0.015	1.677TrpLeu: 1.677 ± 0.037	0.279TrpMet: 0.279 ± 0.015	0.378TrpAsn: 0.378 ± 0.016	0.749TrpPro: 0.749 ± 0.027	0.589TrpGln: 0.589 ± 0.023	1.318TrpArg: 1.318 ± 0.03	0.886TrpSer: 0.886 ± 0.024	0.981TrpThr: 0.981 ± 0.023	0.947TrpVal: 0.947 ± 0.025	0.35TrpTrp: 0.35 ± 0.017	0.33TrpTyr: 0.33 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.549TyrAla: 2.549 ± 0.041	0.179TyrCys: 0.179 ± 0.011	1.397TyrAsp: 1.397 ± 0.036	1.215TyrGlu: 1.215 ± 0.033	0.63TyrPhe: 0.63 ± 0.018	2.262TyrGly: 2.262 ± 0.044	0.373TyrHis: 0.373 ± 0.016	0.426TyrIle: 0.426 ± 0.018	0.316TyrLys: 0.316 ± 0.015	2.146TyrLeu: 2.146 ± 0.039	0.233TyrMet: 0.233 ± 0.013	0.335TyrAsn: 0.335 ± 0.015	1.057TyrPro: 1.057 ± 0.025	0.574TyrGln: 0.574 ± 0.022	1.821TyrArg: 1.821 ± 0.037	0.897TyrSer: 0.897 ± 0.027	1.086TyrThr: 1.086 ± 0.024	1.658TyrVal: 1.658 ± 0.037	0.329TyrTrp: 0.329 ± 0.015	0.4TyrTyr: 0.4 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5132 proteins (1561553 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---