Theobroma cacao (Cacao) (Cocoa)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Theobroma

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061FT68|A0A061FT68_THECC Glycosyltransferase OS=Theobroma cacao OX=3641 GN=TCM_045342 PE=3 SV=1
MM1 pKa = 7.69QPLLKK6 pKa = 10.42NATSLLPFANDD17 pKa = 2.68TMMVVSDD24 pKa = 5.37DD25 pKa = 3.9NAFDD29 pKa = 4.62QMDD32 pKa = 4.07YY33 pKa = 11.5DD34 pKa = 4.13CAKK37 pKa = 11.04DD38 pKa = 5.49DD39 pKa = 3.87TTDD42 pKa = 3.33WNDD45 pKa = 3.18DD46 pKa = 3.75NYY48 pKa = 10.96VGRR51 pKa = 11.84HH52 pKa = 4.69YY53 pKa = 11.45DD54 pKa = 3.91CLNEE58 pKa = 5.5DD59 pKa = 3.99KK60 pKa = 11.25GDD62 pKa = 4.1DD63 pKa = 4.15NSIPNCNHH71 pKa = 5.59VDD73 pKa = 3.49GSTKK77 pKa = 10.18HH78 pKa = 4.78ATIVVLEE85 pKa = 4.12DD86 pKa = 3.25VQYY89 pKa = 11.86DD90 pKa = 3.61DD91 pKa = 3.87ATTVGLEE98 pKa = 4.19DD99 pKa = 3.38VV100 pKa = 4.18

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061GAP0|A0A061GAP0_THECC Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_028269 PE=4 SV=1
MM1 pKa = 7.65PPRR4 pKa = 11.84RR5 pKa = 11.84GLPPIARR12 pKa = 11.84SIGRR16 pKa = 11.84GRR18 pKa = 11.84GRR20 pKa = 11.84SRR22 pKa = 11.84HH23 pKa = 4.89RR24 pKa = 11.84QPNLVRR30 pKa = 11.84GNRR33 pKa = 11.84LRR35 pKa = 11.84LLLRR39 pKa = 11.84QHH41 pKa = 6.92LLLNRR46 pKa = 11.84LRR48 pKa = 11.84LLHH51 pKa = 6.45ILHH54 pKa = 7.17LFHH57 pKa = 7.1HH58 pKa = 6.86LLVFLYY64 pKa = 9.96SCHH67 pKa = 6.22VSS69 pKa = 3.09

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29159

11455

40614

17403952

30

5406

428.5

47.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.708 ± 0.011

1.933 ± 0.007

5.224 ± 0.009

6.514 ± 0.014

4.258 ± 0.009

6.434 ± 0.013

2.37 ± 0.005

5.316 ± 0.01

6.191 ± 0.012

9.899 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.005

4.451 ± 0.008

4.864 ± 0.011

3.813 ± 0.01

5.293 ± 0.009

9.0 ± 0.016

4.772 ± 0.007

6.457 ± 0.008

1.306 ± 0.004

2.758 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski