Beet curly top Iran virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Becurtovirus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7XB51|L7XB51_9GEMI Ss-ds DNA regulator OS=Beet curly top Iran virus OX=391228 GN=V3 PE=4 SV=1
MM1 pKa = 8.0SGMVLDD7 pKa = 4.27WSLYY11 pKa = 10.53DD12 pKa = 3.81IDD14 pKa = 3.83HH15 pKa = 5.88TTYY18 pKa = 10.67HH19 pKa = 6.85IIDD22 pKa = 5.02DD23 pKa = 3.38IRR25 pKa = 11.84YY26 pKa = 9.29QKK28 pKa = 9.96IQQEE32 pKa = 4.35LFKK35 pKa = 11.14SIIGCNEE42 pKa = 4.3DD43 pKa = 3.19YY44 pKa = 11.11SVWIKK49 pKa = 10.7HH50 pKa = 5.77KK51 pKa = 9.99PNLVIPGGRR60 pKa = 11.84PCIAITNPDD69 pKa = 4.09MDD71 pKa = 5.47WIPCMSEE78 pKa = 3.98SMKK81 pKa = 10.99DD82 pKa = 2.94WFYY85 pKa = 11.88ANCDD89 pKa = 3.2VYY91 pKa = 11.63YY92 pKa = 10.6LANDD96 pKa = 3.86EE97 pKa = 4.12VWYY100 pKa = 8.89TT101 pKa = 3.52

Molecular weight: 11.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7XDF9|L7XDF9_9GEMI Capsid protein OS=Beet curly top Iran virus OX=391228 GN=V1 PE=3 SV=1
MM1 pKa = 7.59AVQSQKK7 pKa = 10.4RR8 pKa = 11.84KK9 pKa = 10.22YY10 pKa = 9.69MSATNWAKK18 pKa = 10.31KK19 pKa = 10.17RR20 pKa = 11.84KK21 pKa = 5.06TTTGRR26 pKa = 11.84TVSKK30 pKa = 10.05KK31 pKa = 8.12YY32 pKa = 7.44QWKK35 pKa = 10.13RR36 pKa = 11.84PVRR39 pKa = 11.84SNRR42 pKa = 11.84SVKK45 pKa = 10.6LKK47 pKa = 10.23MYY49 pKa = 10.74DD50 pKa = 3.68DD51 pKa = 4.07MLGASGVGSTISNDD65 pKa = 3.59GMITMLNNYY74 pKa = 7.83VQGIGDD80 pKa = 3.83SQRR83 pKa = 11.84SNNLTVTRR91 pKa = 11.84HH92 pKa = 5.78LKK94 pKa = 10.43FDD96 pKa = 3.68MALMASSMFWEE107 pKa = 4.88RR108 pKa = 11.84PNYY111 pKa = 5.2MTQYY115 pKa = 9.32HH116 pKa = 5.44WVIVDD121 pKa = 3.71RR122 pKa = 11.84DD123 pKa = 3.63VGATFPTQLSTIFDD137 pKa = 3.94IPTGGQAMPSTYY149 pKa = 10.12RR150 pKa = 11.84IRR152 pKa = 11.84RR153 pKa = 11.84DD154 pKa = 3.09ANEE157 pKa = 3.67RR158 pKa = 11.84FIVKK162 pKa = 10.08KK163 pKa = 10.17KK164 pKa = 8.49WQTHH168 pKa = 4.72LMSSGTDD175 pKa = 3.19YY176 pKa = 11.37GGKK179 pKa = 7.59QTYY182 pKa = 8.75KK183 pKa = 10.53APSMPNYY190 pKa = 9.15KK191 pKa = 10.05KK192 pKa = 10.97AMNVNIRR199 pKa = 11.84NINVKK204 pKa = 7.29TQWKK208 pKa = 7.81DD209 pKa = 3.12TGGGKK214 pKa = 10.11YY215 pKa = 10.33EE216 pKa = 4.14DD217 pKa = 3.64VKK219 pKa = 11.28EE220 pKa = 3.89NAILYY225 pKa = 9.01VVVNDD230 pKa = 3.71NTDD233 pKa = 3.47NTNMYY238 pKa = 8.08ATLFGNCRR246 pKa = 11.84AYY248 pKa = 10.51FYY250 pKa = 11.49

Molecular weight: 28.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

863

89

302

172.6

20.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.171 ± 0.806

1.97 ± 0.665

5.678 ± 0.837

6.373 ± 2.348

4.751 ± 0.933

5.214 ± 0.589

1.854 ± 0.516

5.678 ± 1.053

6.257 ± 1.409

7.648 ± 1.56

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.013 ± 1.237

5.446 ± 1.209

4.983 ± 0.898

4.403 ± 0.311

5.794 ± 1.094

7.764 ± 0.836

6.257 ± 1.315

5.562 ± 0.802

2.086 ± 0.527

5.098 ± 0.71
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski