Mycobacterium phage MyraDee

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222YZE8|A0A222YZE8_9CAUD Uncharacterized protein OS=Mycobacterium phage MyraDee OX=2024303 GN=SEA_MYRADEE_63 PE=4 SV=1
MM1 pKa = 7.29PRR3 pKa = 11.84IYY5 pKa = 10.41VASLSDD11 pKa = 3.82YY12 pKa = 11.6NNGDD16 pKa = 3.43LHH18 pKa = 6.85GKK20 pKa = 8.51WFDD23 pKa = 3.79PEE25 pKa = 5.68DD26 pKa = 3.75FDD28 pKa = 6.16FDD30 pKa = 4.83ADD32 pKa = 3.67HH33 pKa = 7.1LYY35 pKa = 10.85EE36 pKa = 4.77AVQAMILDD44 pKa = 4.38GPAAKK49 pKa = 9.78RR50 pKa = 11.84GEE52 pKa = 4.38VAEE55 pKa = 4.3EE56 pKa = 4.01WAIHH60 pKa = 7.07DD61 pKa = 3.8YY62 pKa = 11.72DD63 pKa = 4.47DD64 pKa = 4.95FEE66 pKa = 4.43EE67 pKa = 4.38LRR69 pKa = 11.84ISEE72 pKa = 4.39YY73 pKa = 10.94EE74 pKa = 4.12SFEE77 pKa = 4.51TVCDD81 pKa = 3.66LAKK84 pKa = 10.75LLNEE88 pKa = 5.11LDD90 pKa = 3.52TEE92 pKa = 4.25ARR94 pKa = 11.84PFSLWIDD101 pKa = 4.33AIVGDD106 pKa = 3.77ITYY109 pKa = 10.05FADD112 pKa = 3.01ITEE115 pKa = 4.35AEE117 pKa = 4.0QRR119 pKa = 11.84FRR121 pKa = 11.84DD122 pKa = 4.14AYY124 pKa = 9.97IGEE127 pKa = 4.08QDD129 pKa = 3.45IEE131 pKa = 4.44DD132 pKa = 3.81YY133 pKa = 10.62AYY135 pKa = 10.09EE136 pKa = 4.07YY137 pKa = 11.32AEE139 pKa = 4.12EE140 pKa = 4.14VLGLEE145 pKa = 4.89GVALDD150 pKa = 4.26YY151 pKa = 11.07FDD153 pKa = 4.56SEE155 pKa = 4.17KK156 pKa = 10.43FARR159 pKa = 11.84DD160 pKa = 3.4LEE162 pKa = 4.59LGGDD166 pKa = 3.3ISEE169 pKa = 4.33VEE171 pKa = 4.54GYY173 pKa = 10.41LFHH176 pKa = 7.65ANWW179 pKa = 3.44

Molecular weight: 20.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222YZ09|A0A222YZ09_9CAUD Uncharacterized protein OS=Mycobacterium phage MyraDee OX=2024303 GN=SEA_MYRADEE_45 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.5KK3 pKa = 10.21LLQWLRR9 pKa = 11.84TGHH12 pKa = 5.12WPKK15 pKa = 9.83RR16 pKa = 11.84TRR18 pKa = 11.84PPEE21 pKa = 4.19NVHH24 pKa = 6.26VAADD28 pKa = 3.9PLWRR32 pKa = 11.84RR33 pKa = 11.84DD34 pKa = 3.03GDD36 pKa = 4.12TVTLLFIDD44 pKa = 4.95GGSITYY50 pKa = 8.58TSNRR54 pKa = 11.84RR55 pKa = 11.84EE56 pKa = 4.09HH57 pKa = 6.4GG58 pKa = 3.42

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

15778

23

871

167.9

18.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.386 ± 0.322

0.868 ± 0.106

7.029 ± 0.216

7.029 ± 0.348

3.606 ± 0.155

8.822 ± 0.529

2.034 ± 0.171

4.95 ± 0.151

4.354 ± 0.326

8.265 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.135

3.461 ± 0.166

5.07 ± 0.29

3.594 ± 0.256

6.192 ± 0.288

5.292 ± 0.189

5.641 ± 0.216

6.775 ± 0.21

2.193 ± 0.161

2.922 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski