Brachybacterium faecium (strain ATCC 43885 / DSM 4810 / JCM 11609 / LMG 19847 / NBRC 14762 / NCIMB 9860 / 6-10)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermabacteraceae; Brachybacterium; Brachybacterium faecium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3062 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7MHZ1|C7MHZ1_BRAFD Phosphoglycerate dehydrogenase-like oxidoreductase OS=Brachybacterium faecium (strain ATCC 43885 / DSM 4810 / JCM 11609 / LMG 19847 / NBRC 14762 / NCIMB 9860 / 6-10) OX=446465 GN=Bfae_28970 PE=3 SV=1
MM1 pKa = 7.53SSSGWGGPGNEE12 pKa = 4.15PQGGPGQQGSGQPGGPYY29 pKa = 8.24GTPSPGWQSPPPRR42 pKa = 11.84RR43 pKa = 11.84GRR45 pKa = 11.84SWALLAVAFSCVLALLLVVGGGITYY70 pKa = 10.36LALRR74 pKa = 11.84QNADD78 pKa = 3.3EE79 pKa = 4.68SSVATGSPSTSTTEE93 pKa = 4.04DD94 pKa = 3.27VSPSDD99 pKa = 3.79DD100 pKa = 3.48ASPDD104 pKa = 3.43EE105 pKa = 4.76EE106 pKa = 4.74VPPTPEE112 pKa = 3.67KK113 pKa = 11.09SEE115 pKa = 3.98TSSFEE120 pKa = 3.88VVVPYY125 pKa = 10.3DD126 pKa = 3.93PPTGTVDD133 pKa = 3.73EE134 pKa = 5.15LWDD137 pKa = 3.72VMADD141 pKa = 3.5NPLSEE146 pKa = 4.62GSLPALSTCEE156 pKa = 4.41LPATPTEE163 pKa = 4.26PDD165 pKa = 3.56DD166 pKa = 5.85AEE168 pKa = 4.23LQAVLDD174 pKa = 4.21AASTCLNQVWATASSDD190 pKa = 3.69RR191 pKa = 11.84GLPWTSPEE199 pKa = 3.47IVVYY203 pKa = 8.87HH204 pKa = 6.56HH205 pKa = 7.49PDD207 pKa = 3.13VPKK210 pKa = 10.53EE211 pKa = 4.12ATCSDD216 pKa = 3.43GFAADD221 pKa = 4.78FPRR224 pKa = 11.84VCNLDD229 pKa = 3.46STIYY233 pKa = 10.09WPDD236 pKa = 3.22GYY238 pKa = 10.64GTGRR242 pKa = 11.84DD243 pKa = 3.83LEE245 pKa = 4.59DD246 pKa = 3.89AADD249 pKa = 3.83VPAAYY254 pKa = 9.91LWDD257 pKa = 3.97LSYY260 pKa = 11.41LYY262 pKa = 10.41MNTVTWNSSLAVYY275 pKa = 9.61YY276 pKa = 9.5GTMSTQLEE284 pKa = 4.28EE285 pKa = 4.35TDD287 pKa = 5.04DD288 pKa = 3.95EE289 pKa = 4.68RR290 pKa = 11.84HH291 pKa = 5.84DD292 pKa = 4.14EE293 pKa = 3.57AWRR296 pKa = 11.84RR297 pKa = 11.84YY298 pKa = 8.77NLQNQCLASAASMQVPSAAEE318 pKa = 3.64PSKK321 pKa = 10.24QLRR324 pKa = 11.84DD325 pKa = 3.33ILTSPDD331 pKa = 2.78TWEE334 pKa = 4.5AGQPPKK340 pKa = 10.59TITPEE345 pKa = 3.83NRR347 pKa = 11.84ALWIQRR353 pKa = 11.84GFEE356 pKa = 4.03ADD358 pKa = 3.86GDD360 pKa = 4.09LSACNTWTADD370 pKa = 3.64LEE372 pKa = 4.56QVTT375 pKa = 4.3

Molecular weight: 40.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7MBM7|C7MBM7_BRAFD Predicted aminoglycoside phosphotransferase OS=Brachybacterium faecium (strain ATCC 43885 / DSM 4810 / JCM 11609 / LMG 19847 / NBRC 14762 / NCIMB 9860 / 6-10) OX=446465 GN=Bfae_11380 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.99RR4 pKa = 11.84TFQPNLRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.98KK16 pKa = 9.08HH17 pKa = 4.39GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILNARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.29GRR40 pKa = 11.84SEE42 pKa = 4.21LSAA45 pKa = 4.73

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3062

0

3062

1070770

33

2084

349.7

37.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.407 ± 0.068

0.532 ± 0.008

6.055 ± 0.041

6.583 ± 0.047

2.757 ± 0.028

9.146 ± 0.038

2.305 ± 0.02

3.932 ± 0.031

1.556 ± 0.029

10.758 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.945 ± 0.016

1.573 ± 0.022

5.89 ± 0.037

2.958 ± 0.021

7.64 ± 0.048

5.522 ± 0.031

6.004 ± 0.028

8.224 ± 0.041

1.432 ± 0.017

1.784 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski