Clostridium sp. CAG:242

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2433 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6CXA1|R6CXA1_9CLOT UPF0272 protein Mahau_1643 OS=Clostridium sp. CAG:242 OX=1262783 GN=BN558_01493 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.48RR3 pKa = 11.84MFILIFVAVLLFSLAACGNNEE24 pKa = 4.13GTFSDD29 pKa = 3.52QTINNNLSQSEE40 pKa = 4.4GVDD43 pKa = 3.22NSQVQQIHH51 pKa = 4.96TNILIAYY58 pKa = 8.99DD59 pKa = 3.97PEE61 pKa = 4.25QEE63 pKa = 4.44TITRR67 pKa = 11.84AAKK70 pKa = 10.48LLADD74 pKa = 4.35TLNADD79 pKa = 5.2LIEE82 pKa = 4.33ITNDD86 pKa = 2.78SNLQVDD92 pKa = 4.2TYY94 pKa = 11.32EE95 pKa = 4.17FVLLGFDD102 pKa = 3.91GHH104 pKa = 5.83NQQLSPQVQNFLKK117 pKa = 10.25RR118 pKa = 11.84YY119 pKa = 8.32NFGARR124 pKa = 11.84TIYY127 pKa = 10.39PFVLGDD133 pKa = 4.59GIDD136 pKa = 3.82LSAVSSAISEE146 pKa = 4.41LEE148 pKa = 3.87PGALMGNGEE157 pKa = 4.32FLIEE161 pKa = 4.17EE162 pKa = 4.25NTTDD166 pKa = 3.46EE167 pKa = 5.49EE168 pKa = 4.07IRR170 pKa = 11.84EE171 pKa = 4.23WGAEE175 pKa = 4.08LEE177 pKa = 4.21LSDD180 pKa = 4.89IKK182 pKa = 10.35TAVPTAEE189 pKa = 4.43EE190 pKa = 4.83GNTVATATVTPDD202 pKa = 3.3QQQVLYY208 pKa = 10.54LWEE211 pKa = 4.43EE212 pKa = 4.45DD213 pKa = 3.39NAPAQTEE220 pKa = 4.12YY221 pKa = 11.09TMNDD225 pKa = 2.48GRR227 pKa = 11.84YY228 pKa = 9.63SDD230 pKa = 4.95NPDD233 pKa = 3.07FRR235 pKa = 11.84PYY237 pKa = 8.94LTSYY241 pKa = 9.47PVPEE245 pKa = 4.47GMDD248 pKa = 2.8IKK250 pKa = 10.84GAVLICPGGAFQFRR264 pKa = 11.84SDD266 pKa = 3.47QPEE269 pKa = 4.16GVDD272 pKa = 3.35VAEE275 pKa = 4.7ALNILGYY282 pKa = 9.82QCFVVDD288 pKa = 5.1YY289 pKa = 9.91RR290 pKa = 11.84LQPYY294 pKa = 7.25TQQEE298 pKa = 4.39GALDD302 pKa = 3.87LARR305 pKa = 11.84AVRR308 pKa = 11.84FVRR311 pKa = 11.84SHH313 pKa = 6.85AEE315 pKa = 3.75EE316 pKa = 4.41YY317 pKa = 10.8GIEE320 pKa = 4.15PQDD323 pKa = 3.33IAVMGFSAGGILSGEE338 pKa = 3.95MLINFDD344 pKa = 4.19GDD346 pKa = 3.88VLPTILDD353 pKa = 3.38PEE355 pKa = 4.35YY356 pKa = 10.85QQDD359 pKa = 3.78EE360 pKa = 4.65LDD362 pKa = 3.91SVSADD367 pKa = 3.17VAACGMIYY375 pKa = 10.34SFYY378 pKa = 10.69GQLSLGTTDD387 pKa = 3.56VDD389 pKa = 4.22LLRR392 pKa = 11.84SGDD395 pKa = 4.0LPPTFYY401 pKa = 10.84CYY403 pKa = 9.14GTRR406 pKa = 11.84DD407 pKa = 3.44PFYY410 pKa = 11.23GQFLANADD418 pKa = 3.62AAEE421 pKa = 4.05QAGVLVEE428 pKa = 4.78QLQLDD433 pKa = 4.07NMPHH437 pKa = 6.36GFGAGGDD444 pKa = 3.89WIPAYY449 pKa = 10.22DD450 pKa = 3.36KK451 pKa = 10.32WLTEE455 pKa = 4.06IFSQQ459 pKa = 4.81

Molecular weight: 50.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6CVA1|R6CVA1_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:242 OX=1262783 GN=BN558_00653 PE=3 SV=1
MM1 pKa = 7.34EE2 pKa = 5.09KK3 pKa = 10.53EE4 pKa = 3.55RR5 pKa = 11.84TEE7 pKa = 4.12RR8 pKa = 11.84NDD10 pKa = 2.85RR11 pKa = 11.84RR12 pKa = 11.84NRR14 pKa = 11.84KK15 pKa = 8.07SRR17 pKa = 11.84KK18 pKa = 8.8KK19 pKa = 10.01VCAFCMDD26 pKa = 4.14KK27 pKa = 10.7IEE29 pKa = 4.74NIDD32 pKa = 3.68YY33 pKa = 10.93KK34 pKa = 10.88DD35 pKa = 3.52VPRR38 pKa = 11.84LKK40 pKa = 10.42RR41 pKa = 11.84YY42 pKa = 10.01LSDD45 pKa = 3.23RR46 pKa = 11.84AKK48 pKa = 10.33IIPRR52 pKa = 11.84RR53 pKa = 11.84VTGTCARR60 pKa = 11.84HH61 pKa = 4.44QRR63 pKa = 11.84QLTVAVKK70 pKa = 10.26RR71 pKa = 11.84ARR73 pKa = 11.84HH74 pKa = 4.88VAFLPYY80 pKa = 10.57VGDD83 pKa = 3.67

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2433

0

2433

709936

30

1967

291.8

32.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.526 ± 0.054

1.762 ± 0.023

5.411 ± 0.036

7.26 ± 0.057

4.296 ± 0.04

6.89 ± 0.04

1.838 ± 0.024

6.967 ± 0.046

6.097 ± 0.043

9.556 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.051 ± 0.028

4.05 ± 0.033

3.648 ± 0.026

4.158 ± 0.037

4.313 ± 0.041

6.358 ± 0.05

5.356 ± 0.048

6.884 ± 0.036

0.907 ± 0.016

3.665 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski